High Species Richness Research Articles

Bacterial Diversity in Deep-Sea Sediment of West Pacific Nodule Province

Dense polymetallic nodule fields are found in different areas of the Pacific and Indian Oceans. However, limited knowledge exists about microbial diversity, processes and functions in deep-sea polymetallic nodule sediments. This study investigated microbial diversity, composition and function in sediments from various locations and depths in a western Pacific polymetallic nodule province. Sediment cores were collected, DNA extracted, and the V3–V4 regions of the 16S rRNA gene were sequenced using Illumina MiSeq. The test results show that the abundance and diversity of microbial communities in sediments from different sites vary significantly. The dominant microbial communities at the family level in the three sediment samples were all mainly Marinobacteraceae and Alcanivoracaceae. Sediment samples from core 1 had similar microbial structures and microbial community functions. Surface sediment had higher species richness, diversity and evenness than the middle layer. The dominant phylum at different depths was consistent. There was significant spatial heterogeneity in the microbial community within sediments from polymetallic nodule regions. This study expands on our knowledge of the spatial and vertical distribution of microbial community diversity beneath polymetallic nodules in deep-sea settings.

An annotated checklist of the bees of Washington state

Bees (Hymenoptera: Apoidea) are vital components of global ecosystems, yet knowledge of their distribution is limited in many regions. Washington state is located in an ecologically diverse part of North America and encompasses habitat types and plant communities known for high bee species richness. To establish a baseline for future studies on bee communities in the state, we used published and unpublished datasets to develop a preliminary annotated checklist of bees occurring in Washington state. We document, with high confidence, 565 species of bees in Washington and identify an additional 102 species likely to occur in the state. We anticipate future research survey efforts, such as the newly initiated Washington Bee Atlas, will discover several species that have the potential to occur in Washington and provide new data for 84 species which have not been recorded in more than 50 years.

Two centuries of ornithological exploration of the Rio Doce Basin, southeastern Brazil. Part II—A catalogue of its birds and a gazetteer

The Rio Doce Hydrographic Basin (RDB) lies almost completely in the Brazilian Atlantic Forest, a highly threatened tropical rainforest. The RDB has suffered dramatic anthropogenic impacts during the last two centuries and is currently one of the most degraded regions in southeastern Brazil. In this paper, we gathered 140,742 bird records collected since the early 19th by more than two thousand naturalists, professional scientists, and citizen scientists. This vast database was compiled from several sources, including published literature, unpublished technical reports, natural history museums, and citizen science platforms. After checking and standardizing the database, we found high species richness in the RDB, with 689 species recorded to date, from which 675 (98%) were documented by specimens or digital vouchers. This database is presented as a detailed catalogue, including maps containing all localities in which each species has been recorded. We present text accounts for 40 noteworthy species recorded in the RDB, and a complete gazetteer for the 636 localities sampled. Preliminary analyses reveal that the RDB avifauna suffered a dramatic change in its species composition during the last two centuries, mostly due to human activity. Highly sensitive species associated to pristine forests vanished, while species associated to dry and open habitat expanded their ranges in the RDB. Finally, in addition to thoroughly characterization of the RDB avifauna, this paper provides the basis for a series of studies about the avifauna of one of the hottest hotspots of biodiversity in the entire Neotropical region.

Exploring riverine aquatic animal diversity and establishing aquatic ecological monitoring approaches tailored to the Qinling region via eDNA technology.

This study thoroughly examines biodiversity and aquatic ecosystems across 14 sampled sites within the Shitou River basin by utilizing environmental DNA technology. Through integrated analysis and high-throughput sequencing, the study elucidates a diverse array of biodiversity, encompassing 27 fish species and 341 freshwater benthic macroinvertebrates (FBM) species. Using various biodiversity indices, we found significant differences in diversity and stability across different environments. Regions with more complex habitats had higher species richness and evenness. Further analyses showed complex relationships between diversity metrics for FBM and fish, indicating potential interactions between these groups. The standardized mean score (SMS) was developed to aid in the assessment of water quality. Specifically, SMS scoring revealed that sites STH3, STH4, and STH14 excelled across multiple dimensions, earning an "Excellent" rating, while site STH12 was rated as "Poor" due to subpar performance across several metrics. This project not only enhances current understanding regarding aquatic ecological dynamics but also establishes a strong foundation for detailed environmental evaluation and monitoring, aligned with the priorities of contemporary ecological management and caution.

Polyploidy linked with species richness but not diversification rates or niche breadth in Australian Pomaderreae (Rhamnaceae).

Polyploidy is an important evolutionary driver for plants and has been linked with higher species richness and increases in diversification rate. These correlations of ploidy with plant radiations could be the result of polyploid lineages exploiting greater niche space and novel niches due to their enhanced adaptability. Ploidy evolution and how it links with diversification of plants across the Australian continent is not well understood. Here, we focused on the ploidy evolution of the Australasian Rhamnaceae tribe Pomaderreae. We generated a densely-sampled phylogeny (90%, 215/240 spp.) of the tribe and used it to test for the evolution of ploidy. We obtained 30 orthologous nuclear loci per sample and dated the phylogeny using TreePL. Ploidy estimates for each sequenced species was obtained using nQuire, based on phased sequence data. We used MiSSE to obtain tip diversification rates and tested for significant relationships between diversification rates and ploidy. We also assessed for relationships between ploidy level and niche breadth, using distributional records, species distributional modelling, and WorldClim data. Polyploidy is extensive across the tribe, with almost half (45%) of species and majority of genera exhibiting this trait. We found a significant positive relationship between polyploidy and genus size (i.e., species richness), but non-significant positive relationship between polyploidy and diversification rates. Polyploidy did not result in significantly wider niche space occupancy for Pomaderreae, however polyploidy did allow for transitions into novel wetter niches. Spatially, eastern Australia is the diversification hotspot for Pomaderreae in contrast to the species hotspot of southwest Western Australia. The relationship between polyploidy and diversification is complex. Ancient polyploidisation events likely played an important role in the diversification of species rich genera. A lag time effect may explain the uncoupling of tip diversification rates and polyploidy of extant lineages. Further studies on other groups are required to validate these hypotheses.

Amazonian conservation across archipelagos of Indigenous territories.

Indigenous stewardship is essential to the conservation of biocultural diversity, yet conventional conservation models often treat Indigenous territories (ITs) as homogeneous or isolated units. We propose that archipelagos of Indigenous territories (AITs), clusters of ITs that span geographies but are connected through shared cultural or political ties maintained by Indigenous nations, are crucial for understanding and enhancing conservation strategies that recognize the complexity of Indigenous stewardship. We classified 3572 ITs in the Amazon into 4 categories-single or multiple nations with either singular IT or AIT-to assess their spatial heterogeneity, governance, and conservation potential. We then assessed species richness, carbon stocks, and pressures across these different categories. To examine how AITs can enhance biocultural conservation efforts, we conducted a case study of the Cofán Nation in Ecuador. AITs covered 45% of the Amazonian land area and had higher species richness and carbon stocks than single IT configurations. However, AITs faced greater pressures from development and extractive activities. In the case study, the Cofán AIT was shaped by colonization and land titling challenges, but their community-driven governance, cross-territorial collaboration, and adaptive responses-such as comanagement agreements and resisting extractive activities-enhanced their ecological and cultural resilience amid growing development pressures. Our findings suggest that AITs facilitate the exchange of resources, knowledge, and cultural practices, which strengthens social connectivity, reinforces governance structures, and enables adaptive management across ITs, thereby enhancing biocultural resilience across discontinuous spaces. This work advocates for a paradigm shift in conservation planning and practice that recognizes the vital role of AITs in sustaining Amazonian ecosystems and Indigenous lifeways, particularly in the face of increasing pressures.

Seagrass Biodiversity Under the Latest‐Generation Scenarios of Projected Climate Change

ABSTRACTAimThe potentially cascading consequences of climate changes on redistribution of habitat‐forming species, like seagrasses, remain a major research gap. Empirical demonstrations of local population changes are increasingly reported without a globally integrated predictive framework as a leading testable hypothesis. Therefore, here, we aimed to estimate changes in species richness, community composition, and areas of climatic refugia under future climate scenarios.LocationGlobal scale.Time PeriodPresent‐day conditions (from 2010 to 2020) and for three Shared Socioeconomic Pathway (SSP) scenarios of future climate change (from 2090 to 2100).Major Taxa StudiesSeagrasses (plantae).MethodsWe coupled seagrass occurrences with environmental predictors (temperature, salinity, nitrate, wave energy, and ice) in stacked species distribution modelling.ResultsModels estimated a present global extent of 917,169 km2 with high species richness in Temperate Australasia, Indo‐Pacific, and Temperate North Pacific. Future projections predicted widespread spatial redistribution, with Arctic expansions, losses in lower latitudes, and deeper vertical ranges, while globally maintaining the area extent occupied worldwide by seagrass species (only 5% of change). Species richness increased poleward under more drastic scenarios (SSP3‐7.0 and SSP5‐8.5), with losses in tropical zones (30oN to 30oS). Local climatic refugia are retained in all scenarios but decrease under higher emissions. Additionally, even where seagrass species remain, widespread community composition changes were predicted.Main ConclusionsOur findings serve as baselines to inform, anticipate, and mitigate cascading consequences of shifts in seagrass ecosystems that provide essential services for humanity.

Independent effects of tree diversity on aboveground and soil carbon pools after six years of experimental afforestation.

Planting diverse forests has been proposed as a means to increase long-term carbon (C) sequestration while providing many co-benefits. Positive tree diversity-productivity relationships are well established, suggesting more diverse forests will lead to greater aboveground C sequestration. However, the effects of tree diversity on belowground C storage have the potential to either complement or offset aboveground gains, especially during early stages of afforestation when potential exists for large losses in soil C due to soil decomposition. Thus, experimental tests of the effects of planted tree biodiversity on changes in whole-ecosystem C balance are needed. Here, we present changes in above- and belowground C pools 6 years after the initiation of the Forests and Biodiversity experiment (FAB1), consisting of high-density plots of one, two, five, or 12 tree species planted in a common garden. The trees included a diverse range of native species, including both needle-leaf conifer and broadleaf angiosperm species, and both ectomycorrhizal and arbuscular mycorrhizal species. We quantified the effects of species richness, phylogenetic diversity, and functional diversity on aboveground woody C, as well as on mineral soil C accumulation, fine root C, and soil aggregation. Surprisingly, changes in aboveground woody C pools were uncorrelated to changes in mineral soil C pools, suggesting that variation in soil C accumulation was not driven by the quantity of plant litter inputs. Aboveground woody C accumulation was strongly driven by species and functional identity; however, plots with higher species richness and functional diversity accumulated more C in aboveground wood than expected based on monocultures. We also found weak but significant effects of tree species richness, identity, and mycorrhizal type on soil C accumulation. To assess the role of the microbial community in mediating these effects, we further compared changes in soil C pools to phospholipid fatty acid (PLFA) profiles. Soil C pools and accumulation were more strongly correlated with specific microbial clades than with total microbial biomass or plant diversity. Our results highlight rapidly emerging and microbially mediated effects of tree biodiversity on soil C storage in the early years of afforestation that are independent of gains in aboveground woody biomass.

Species richness of Hymenopteran flower visitors in Brassica campestris var. toria in Assam, India: A comparison of five sampling methods

AbstractWe studied the species richness of flower-visiting Hymenoptera assemblages in two varieties of Brassica campestris var. toria: in variety TS-36 during the normal season grown in the riparian ecosystem and in variety TS-67 during the late season grown in the rice-ecosystem of Assam, India using five sampling methods. We aimed to investigate to what extent (i) the Hymenoptera assemblages in the two ecosystems differed from each other, (ii) the population densities of three dominant Apis spp. differed from each other, and (iii) the effectiveness of the sampling methods used differed from each other. In total, we recorded 64 Hymenoptera species belonging to 42 genera of 9 families in the two toria varieties. We detected the presence of all species in TS-36 grown as normal season crop in the riparian ecosystem, and the presence of 48 species in TS-67 grown in the rice ecosystem as a late season crop. Vespidae had the highest species richness, followed by Apidae, Megachilidae, and Halictidae. These visitors may contribute to the ecological resilience of the toria ecosystem through their service as pollinators as well as because they are natural enemies of the crop-pests. Transect walk methods sampled a higher number of species than observation plot method and colour traps. Apis cerana was the most abundant species, with a relative abundance of 38.5% and 37.9% in normal and late season crops, respectively. The mean abundance of all three Apis species was 11.1% higher in the later variety.

Bioprospecting for sustainable and eco-friendly bioproducts: A case study of multi-enzyme production by soil microbes

Microbial multi-enzyme production is a promising research area involving microbes that can simultaneously synthesize numerous enzymes for the degradation of substrates with environmental sustainability and other industrial applications as the focus. During this study, we isolated, screened, and identified some multi-enzyme-producing microbes from three soil sites in Ibadan: Apete Dumpsite (ADS); Botanical Garden University of Ibadan- (BGUI); and Eleyele Cassava Processing Field- (ECPF). Precisely 500 g of soil were collected per point at different depths (0–10, 10–20, and 20–30 cm) and further analyzed. The microbial load and type and enzyme assays were determined. The highest average microbial count (6.9 × 108 CFU/g) was recorded in the ECPF soil sample at 0–10 cm depth, while the lowest microbial count of 3.3 × 105 CFU/g at 20–30 cm soil depth in BGUI. Multi-enzyme-producing microbes were identified phenotypically and molecularly. This study revealed an inverse relationship between microbial community composition and soil depth. Bacterial and fungal genera had higher species richness, biodiversity, and evenness than yeast from all sampled points. With significant amylolytic, proteolytic, lipolytic, cellulolytic, and pectinolytic activities, 22 of the 193 isolates were identified as multienzyme producers. The blasted 16S rRNA and ITS rRNA amplicon sequences compared with sequences in the NCBI gene bank identified Bacillus, Roseomonas, Aeromonas, Phanerochaete, Aspergillus, Fusarium, Trametes, and Rigidoporus species. Results from this study show that topsoils (0–10 cm), rich with organic matter, are good reservoirs of multienzyme-producing microorganisms- applicable for environmental sustainability and in other important industries for waste management, food processing, and biomaterial production.

Exploring Floristic Diversity, Propagation Patterns, and Plant Functions in Domestic Gardens across Urban Planning Gradient in Lubumbashi, DR Congo

Urbanization degrades natural habitats and creates new urban ecosystems like domestic gardens. The plant composition of these gardens varies with socio-economic factors and urban planning levels. However, the diversity and impact of introduced species are often poorly assessed, causing potential ecological imbalances (disruptions in the natural functioning and stability of ecosystems), particularly in Lubumbashi (DR Congo). The objective was to analyze the spatial structure, plant diversity, propagation strategies, and ecological functions of domestic gardens. Three distinct neighborhoods were selected: a planned, unplanned, and residential neighborhood. Twenty avenues (with five plots per avenue) were chosen to represent the diversity within each neighborhood, and stratified random sampling of plots was conducted to analyze gardening practices. Gardens were classified into types, and their vegetation was evaluated based on species origin and ecological impact. The analysis of domestic gardens in Lubumbashi reveals significant variations across different neighborhood types. Residential neighborhoods exhibit larger average garden sizes (315.1 m2), higher species richness (22 species), and larger plot sizes (1032 m2) compared to unplanned and planned neighborhoods, where garden areas and species richness are notably lower. Rectangular gardens dominate in unplanned areas, while planned neighborhoods feature more intentional landscaping elements, such as flowerbeds and hedges. The use of gardens for food production is prominent in planned areas (40.7%), whereas residential neighborhoods prioritize ornamentation (51.4%). The study identified 232 taxa across 68 families, with a predominance of exotic species (80%) in all neighborhoods, particularly in unplanned areas (82.25%). The data revealed that Mangifera indica and Persea americana are abundant in all neighborhoods, illustrating their adaptability to different urban contexts. Herbaceous species are most common, followed by woody plants, with vines being sparse. Species dispersal is primarily driven by human activities (anthropochory), accounting for over 85% in all neighborhoods. These findings highlight the strong human influence on the composition and structure of domestic gardens in Lubumbashi, emphasizing the dominance of exotic species and the importance of anthropogenic factors in shaping urban green spaces. Urban policies should incorporate strategies to minimize the negative impacts of exotic species on native flora.

Street tree communities reflect socioeconomic inequalities and legacy effects of colonial planning in Nairobi, Kenya

Street trees provide ecological and social benefits that sustain urban life, yet their distribution frequently mirrors socioeconomic inequalities, meaning underprivileged social groups also have fewer trees. The distribution patterns of urban trees are least documented in the cities of rapidly evolving middle-income countries. We assessed whether street tree abundance, size, condition, diversity, and composition vary across census-derived socioeconomic strata in Nairobi, Kenya. We sampled 2047 trees across 12 neighborhoods, covering 24 km of street, then used linear regression to understand how socioeconomic strata relate to street tree characteristics. We found substantial disparities in tree abundance, with affluent areas harboring 91.5 % of the trees sampled. Low-income areas and informal settlements had comparably few trees. Mean diameter and condition did not vary across socioeconomic strata, but high-income, formerly European neighborhoods had a higher proportion of small trees, indicating a bias in recent urban greening investments further benefiting these areas. Species diversity followed a similar pattern of inequality. High-income neighborhoods had over 30 % higher species richness and diversity than low-income areas. Even so, lower income neighborhoods exhibited greater differences in street tree community composition, and a higher proportion of trees that bear edible fruit or are used in traditional medicine. Overall, our results reveal pronounced spatial inequality in the distribution of street trees in Nairobi, reflecting not only socioeconomic differences but the enduring legacies of colonial planning.

Bee Diversity and Abundance During Peach Bloom in South Carolina, United States

ABSTRACTPeach (Prunus persica [L.] Batsch) is an important speciality fruit crop in the United States. Peach is self‐compatible, but bee pollination enhances fruit sets and diverse populations improve pollination efficiency. However, the bee diversity and abundance in peach orchards in South Carolina are unknown. To understand this, we sampled bees during peach tree bloom at two locations in South Carolina—the Clemson University Musser Fruit Research Center (MFRC) and a peach farm in Ridge Spring, South Carolina. Overall, a total of 651 bees were collected from peach orchards, of which Bombus (37%) and Apis (32%) were the most dominant genera, followed by Eucera (14%), Andrena (8%) and Habropoda (5%). Diversity indices indicate higher species richness and diversity at the Ridge Spring farm than at MFRC. Dominant species at the MFRC were Apis mellifera (36.77%), Bombus bimaculatus (29.1%), B. griseocolis (12%) and Eucera hamata (8%). In contrast, the Ridge Spring farm was dominated by A. mellifera (24.17%), Habropoda laboriosa (12%), Andrena barbara (12%), E. atriventris (11.72%) and B. bimaculatus (11.35%). In comparing the effectiveness of sampling methods, blue vane traps (BVTs) captured more bees than aerial sweep netting. Furthermore, BVTs predominantly captured larger‐sized bees, while smaller‐sized bees were more frequently collected using sweep netting. Throughout peach bloom, differences in bee abundance were observed over time as well. Our study sheds light on the bee diversity and abundance in South Carolina peach orchards, revealing variations between locations. This knowledge of bee diversity and abundance will provide baseline information on bee communities in peach orchards and will help develop suitable pollinator conservation programs.

Edinburgh postpartum depression scores are associated with vaginal and gut microbiota in pregnancy

BackgroundPrenatal and postpartum depression may be influenced by the composition of host associated microbiomes. As such, the objective of this study was to elucidate the relationship between the human gut or vaginal microbiomes in pregnancy with prenatal or postpartum depression. Methods140 female participants were recruited at their first prenatal visit and completed the Edinburgh Postnatal Depression Scale (EPDS) to screen for depression and anxiety, in addition the EPDS was completed one month postpartum. Vaginal and stool biospecimens were collected in the third trimester, analyzed using 16S rRNA gene sequencing, and assessed for alpha and beta diversity. Individual taxa differences and clustering using the k-medoids algorithm enabled community state type classification. ResultsParticipants with higher postpartum EPDS scores had higher species richness and lower abundance of L. crispatus in the vaginal microbiota compared to those with lower EPDS scores. Participants with a higher prenatal EPDS score had lower species richness of the gut microbiome. Participants with a vaginal community state type dominated by L. iners had the highest mean prenatal EPDS scores, whereas postpartum EPDS scores were similar regardless of prenatal vaginal state type. LimitationsOur small sample size and participant's self-report bias limits generalizability of results. ConclusionsDepression in the prenatal and postpartum period is associated with the composition and diversity of the gut and vaginal microbiomes in the third trimester of pregnancy. These results provide a foundational understanding of the microbial relationships between maternal health and depression for identifying potential therapeutic treatments.

Protected and Unprotected Areas as Refuges for Bird Conservation in Southeastern Coahuila, Mexico

Information gaps about the distribution of species hamper the evaluation of conservation status and decisions on biodiversity conservation, affecting areas with high species richness and endemism to a greater extent. In this context, carrying out biological inventories in protected and unprotected areas is an important tool to fill these gaps by providing data on the composition, richness, and abundance of species in each locality. The southeastern region of Coahuila (northeast Mexico) is a region where biodiversity research is required, as anthropogenic activities pose a serious threat to the avian diversity of this region. The present study aimed to compare the capacity of protected and unprotected areas to support the richness, abundance, and composition of bird species, as well as providing a list of bird species from eight areas located in this region. The point count method was conducted at the sites, and one-way ANOVA was used to determine the effect of area type on species richness and abundance. Based on our field observations, literature review, and digital databases, the avifauna of these eight areas includes 388 species in 63 families. Of these, 5 species are endemic to Mexico, 6 species are of global conservation concern, and 35 species are of regional conservation concern. The species richness found in this study supports the importance of protected and unprotected areas in southeastern Coahuila. The results of our study highlight the need for further assessment to conserve the avian community, especially considering the increasing threats of anthropogenic disturbance, as well as additional studies that will allow for better characterization of the avifauna of the region. This will help to ensure the conservation of the birds currently threatened by strong anthropic pressure due to the conversion of semiarid ecosystems to agricultural and livestock areas as well as increasing urbanization.

Diversity of tree flora in the forest galleries of four ponds in the Comoé National Park (north-east Ivory Coast)

The riparian forest galleries of the ponds in the Comoé National Park (CNP) constitute microhabitats colonised by a specific plant and animal community. These plant formations are of great research interest. These initiatives could lead to efficient management of these biomes in the short, medium and long term. This study is part of an effort to make the most of plant resources in forest galleries. The aim of this study is to assess the diversity of riparian species in the ponds of the CNP. The information was collected using the surface survey method combined with the roving inventory method. The inventory identified 78 species divided into 63 genera and 30 families. The buffalo pond forest gallery has the highest species richness, with 39 species in 33 genera and 19 families. The study also showed that for trees with DBH ≥10 cm, the species Diospyros mespiliformis (25.89%), Mitragyna inermis (17.11%), Khaya senegalensis (12.27%), Piliostigma thonningii (11.65 %), Cola gigantean (11.16 %), Vitellaria paradoxa (10.90 %) and Zanthoxylum zanthoxyloides (10.84%)) are the most abundant in riparian pond formations. The Fabaceae and Rubiaceae families are the most represented in terms of number of species, demonstrating that the flora of the forest galleries in Comoé National Park is of major ecological interest.

Reptilian Diversity in Selected Riparian Systems in Misamis Occidental, Philippines

Reptiles are one of the most ecologically important organisms in the ecosystem. Studying their diversity is important in the conservation of the habitats to where each of these reptiles live. This study aimed to explore the diversity of reptiles in the areas around and within Labo and Clarin rivers, two of the most important river systems in the province of Misamis Occidental. Three sampling techniques were applied, which are visual encounter surveys, opportunistic encounters, and road-kill encounters. The results recorded a total of 21 species, including eleven endemic species, with nine Philippine endemic and two Mindanao endemic. Most notable among the species recorded was Lycodon ferroni, which is the second documented occurrence of the species and the first outside Samar Island. Labo River has the highest species richness, abundance and percent endemism of the two riparian systems, with the upstream sites of both rivers having the highest species richness and percent endemism. Studying the nearby riparian areas using other sampling techniques is recommended to fully understand the extent of reptilian diversity in the province and to effectively implement conservation and sustainability programs for the preservation not only of the reptile diversity but as well as the riparian ecosystem.

Evaluating urban biodiversity: Effectiveness of citizen science driven species distribution models in urban ecosystems

Citizen science (CS) and remote sensing (RS) approaches have become more reliable, thus providing higher resolution and generating a large amount of environmental data. When considering urban environments, where fragmented and highly diverse landscapes are predominant, the combination of citizen science data and remote sensing techniques with species distribution models (SDM) can play a vital role in comprehensively investigating and evaluating urban biodiversity. However, citizen science derived species distribution models for multiple avian species in dense and fragmented urban areas are rarely used. The study aims (I) to elaborate, whether CS driven SDMs can be effectively used in spatially complex urban environments; (II) to identify biodiversity hotspots and prioritize areas for nature conservation and (III) to examine, if existing protective areas correspond to species’ hotspots. Therefore, Citizen science-based datasets of 26 breeding bird species over three years were obtained for this analysis in Germany’s Ruhr Metropolis. Quality assurance, data thinning, and pseudo-absence simulations were performed. Spatial data from the ecosystem LiDAR project GEDI (Global Ecosystem Dynamics Investigation), climate data from the German Weather Service, and land use information from Copernicus were used as environmental predictors. Eleven different species distri-bution models (SDMs) were trained on species subselection by using Biomod2 for preliminary analysis. Overall model performance was evaluated via several metrics, including TSS (true skill statistics) and ROC (receiver operating characteristic). Finally, four species distribution models were used for ensemble modelling. Subsequently, a species richness analysis was performed with the aim of identifying spots with high avian biodiversity. Overall, the CS-derived SDMs performed well, with high predictive power for all of the investigated species. Within the Ruhr Metropolis, approximately 6% (250 km²) of the terrain was identified as being highly suitable for avian diversity, inhabiting at least 17 out of 26 species. Predominantly within the core urban areas, high species richness was predicted on preserved brownfields and revitalized mine sites, as well as in the remnants of formerly demarcated regional greenbelts. Additionally, regions outside of the core area, which are part of the overarching biotope network framework, proved to have high species richness capabilities for avian biodiversity. These findings aid in optimizing urban development concepts and (sub)urban green space management with respect to urban biodiversity conservation. Following the implications of the recently established Regional Biodiversity Strategy in the Ruhr Metropolis, this analysis demonstrates the importance of networked green spaces, their preservation and the need to close existing network gaps within the Ruhr Metropolis.

Microbial Community Structure and Metabolic Function in the Venom Glands of the Predatory Stink Bug, Picromerus lewisi (Hemiptera: Pentatomidae).

The predatory stink bug, Picromerus lewisi (Hemiptera: Pentatomidae), is an important and valuable natural enemy of insect pests in their ecosystems. While insects are known to harbor symbiotic microorganisms, and these microbial symbionts play a crucial role in various aspects of the host's biology, there is a paucity of knowledge regarding the microbiota present in the venom glands of P. lewisi. This study investigated the venom glands of adult bugs using both traditional in vitro isolation and cultural methods, as well as Illumina high-throughput sequencing technology. Additionally, the carbon metabolism of the venom gland's microorganisms was analyzed using Biolog ECO metabolic phenotyping technology. The results showed 10 different culturable bacteria where the dominant ones were Enterococcus spp. and Lactococcus lactis. With high-throughput sequencing, the main bacterial phyla in the microbial community of the venom glands of P. lewisi were Proteobacteria (78.1%) and Firmicutes (20.3%), with the dominant bacterial genera being Wolbachia, Enterococcus, Serratia, and Lactococcus. At the fungal community level, Ascomycota accounted for the largest proportion (64.1%), followed by Basidiomycota (27.6%), with Vishniacozyma, Cladosporium, Papiliotrema, Penicillium, Fusarium, and Aspergillus as the most highly represented fungal genera. The bacterial and fungal community structure of the venom glands of P. lewisi exhibited high species richness and diversity, along with a strong metabolism of 22 carbon sources. Functional prediction indicated that the primary dominant function of P. lewisi venom-gland bacteria was metabolism. The dominant eco-functional groups of the fungal community included undefined saprotroph, fungal parasite-undefined saprotroph, unassigned, endophyte-plant pathogen, plant pathogen-soil saprotroph-wood saprotroph, animal pathogen-endophyte-plant pathogen-wood saprotroph, plant pathogen, and animal pathogen-endophyte-epiphyte-plant pathogen-undefined saprotroph. These results provide a comprehensive characterization of the venom-gland microbiota of P. lewisi and demonstrate the stability (over one week) of the microbial community within the venom glands. This study represents the first report on the characterization of microbial composition from the venom glands of captive-reared P. lewisi individuals. The insights gained from this study are invaluable for future investigations into P. lewisi's development and the possible interactions between P. lewisi's microbiota and some Lepidopteran pests.

A Checklist of Butterflies in Ananda Tea Estate and its Adjacent Areas of Lakhimpur District, Assam, India

In the present study, a checklist of butterflies from the Ananda Tea Estate of Lakhimpur district, Assam is prepared and presented. During the study, surveys were carried out from October 2022 to April 2024 in the Ananda tea estate (27°27' N and 94°14' E) and its nearby areas– Dulung reserve forest and Subansiri river. A total of 36 species across 32 genera belonging to five families viz., Nymphalidae, Pieridae, Papilionidae, Lycaenidae, Riodinidae were recorded during the study. Amongst all the families, Nymphalidae was the most diverse family comprising 17 species which belong to 16 different genera. Approximately 48% of the recorded species belong to the Nymphalidae family, indicating the highest species richness. The dominant nature of the family Nymphalidae can be attributed to the polyphagous nature of the species under the family which help them to live in diverse habitats. The species Common albatross (Appias albina), recorded during the study, is protected under Schedule II of the Wildlife (Protection) Amendment Act, 2022 of India. The Whittaker β-diversity was found to be 0.6875, indicating a moderate level of species turnover among the study sites. In the tea estate, due to the spraying of chemical pesticides large number of butterflies die each year which can be saved by replacing the chemical pesticides with biopesticides and other eco-friendly pest control methods including use of botanicals, Indigenous Technical Knowledge (ITKs) and microbial antagonists.