High Production Research Articles

CoMo/g-C3N4 as a highly effective, reusable, non-noble metal-based catalyst for H2 production via NaBH4 hydrolysis focused on stationary application under mild conditions: A kinetic study

Among hydrogen storage chemicals for proton exchange membrane fuel cells (PEMFCs), sodium borohydride stands out for yielding exceptionally pure hydrogen. This study synthesized cobalt-molybdenum catalysts supported on g-C3N4 using an ultrasonic-assisted impregnation method. Various CoMo/g-C3N4 catalysts with different CoMo mass ratios were created, all showing high hydrogen production and complete NaBH4 conversion under mild conditions. Catalytic activity depends on the cobalt-molybdenum mass ratio, with optimal performance at a 1:1 ratio (w/w), maintaining up to 83% activity over 5 cycles. The effect of operating conditions on hydrogen release was explored, resulting in the rate law: rH2 = 1.708 × 10-3 [NaBH4]1.25 [Catalyst dosage]1.39 [NaOH]0.44 at 298 K, with a low activation energy of 22.9 kJ mol−1. Molybdenum's promoting effect prevented significant deactivation of the cobalt catalyst, ensuring complete NaBH4 conversion to H2 and good stability over multiple cycles.

The hypothalamic transcriptome reveals the importance of visual perception on the egg production of Wanxi white geese

Egg performance significantly impacts the development of the local goose industry. The hypothalamus plays an essential role in the egg production of birds. However, few potential candidate genes and biological functions related to egg production in geese have been identified in hypothalamus tissue. In this study, 115 geese were raised and observed for 5 months during the laying period. To understand the regulation mechanism of egg production, the hypothalamus transcriptome profiles of these geese were sequenced using RNA-seq. The hypothalamus samples of four high egg production (HEP) and four low egg production (LEP) geese were selected and collected, respectively. A total of 14,679 genes were identified in the samples. After multiple bioinformatics analyses, Gene Ontology (GO) annotations indicated that genes related to egg production were mainly enriched in biological processes of “response to light stimulus,” “sensory system development,” and “visual perception.” Six potential candidate genes (PDE6C, RHO, MFRP, F2, APOB, and IL6) based on their corresponding GO terms and interaction networks were identified. These identified candidate genes can be used as selection markers to improve the egg production of Wanxi white geese. Our study highlights how visual perception may affect the regulation of geese egg production.

Miocene Climatic Optimum and Middle Miocene Climate Transition: a foraminiferal record from the central Ross Sea, Antarctica

Abstract. The Ross Sea record of the Miocene Climatic Optimum (MCO; ∼ 16.9–14.7 Ma) and the Middle Miocene Climate Transition (MMCT; ∼ 14.7–13.8 Ma) provides critical insights into Antarctic ocean–cryosphere interactions during a time of extreme warmth and subsequent cooling. Here we report on Lower to Middle Miocene foraminiferal assemblages from the International Ocean Discovery Program (IODP) Site U1521 on the outer shelf of the central Ross Sea to identify regional shifts in environmental and water mass conditions and trace continental shelf evolution. We identified seven benthic biofacies clusters, dominated by abundant Globocassidulina subglobosa (a proposed indicator of proto-Circumpolar Deep Water, pCDW), Uvigerina cf. U. fueguina (high productivity and enhanced bottom-water currents), Nonionella spp. (high productivity), or Melonis spp. (high productivity) using a Q-mode cluster analysis to develop preliminary regional paleoenvironmental interpretations. Four unique assemblages, including Globobulimina cf. G. auriculata (high productivity and low oxygen), are also identified. Unit IV (representing the early MCO event) is a short-lived (∼ 80 ka), progradational, clast-poor sandy diamictite, likely deposited during deglaciation; the upper part of Unit IV is transitional with overlying Unit III. Unit IV sediments contain the most persistently abundant and diverse foraminiferal assemblages recovered at U1521 because they are mud-rich and diatom-poor, despite very high sedimentation rates. The benthic assemblages shift between Globocassidulina and Uvigerina dominance, suggesting changes in the pCDW influence relative to productivity and/or current activity. We suggest the abundance of Uvigerina (a shelf-edge proxy) in Unit IV records the northward progradation of the Ross continental shelf at this location during the late Early to Middle Miocene. Unit III (MCO) was deposited in an open-marine setting, evident by the ice-rafted detritus or debris (IRD) clast-free, diatom-rich/diatom-bearing muds. The sporadic nature of foraminiferal abundances in Unit III is likely due to intervals of terrigenous mud alternating with more diatom-rich/diatom-bearing muds. As in Unit IV, the muddier lithologies (higher natural gamma ray (NGR) values) are more likely to preserve calcareous foraminifera, whereas the most diatom-rich sediments (lower NGR values) are more corrosive to carbonate. We interpret the muddier intervals as interglacials with incursions of pCDW, as indicated by increased Globocassidulina subglobosa, and sporadic occurrences of rare warmer-water planktic foraminifera. Collectively, these multiple incursions of warmer-water planktic foraminifera provide evidence for polar amplification in the Ross Sea during the MCO and MMCT. The diatom-rich muds are interpreted as glacials during the MCO with open-marine conditions and higher productivity. The dominance of Globobulimina in the upper part of Unit III corresponds with the carbon maximum of Carbon Maxima 2 (CM2) and low-oxygen conditions in the sediments at ∼ 16.1 Ma. Subsequent glaciation (including Mi2, Miocene Isotope event 2), marine-based ice sheet grounding, and erosion on the shallow shelf are recorded by the widespread Ross Sea Unconformity 4 (RSU4; ∼ 15.95–14.2 Ma) at Site U1521. Unit II (MMCT) likely represents sedimentation in the interval between the RSU4 and the Mi3 (Miocene Isotope event 3) glaciation at ∼ 13.9–13.8 Ma. The benthic biofacies composition of Unit II shows a further increase in neritic taxa, including Elphidium magellanicum and Epistominella vitrea, suggesting continued shoaling of the continental shelf, which facilitated the growth of marine-based ice sheets during the Middle Miocene. Our initial correlation between Site U1521 and the ANtarctic geological DRILLing Project (ANDRILL) site, AND-2A, yields similar environmental interpretations, including peak warm events 3 and 4 during the MCO, supported by the foraminifera and unit lithologies. Suspected glacial intervals during the MCO, including Mi2 at the top of Unit III, correlate well with the reconstructed deep-sea estimates of ice volume changes (seawater δ18Osw record) from the Ocean Drilling Program (ODP) Site 1171 on the South Tasman Rise.

A photocatalysis-self-Fenton system based on NCDs@ZnIn2S4 composites at neutral pH and low amount of Fe2+ for the effective degradation of antibiotics

Photocatalysis-self-Fenton combining photocatalytic production of H2O2 with Fenton reaction has been a hotspot, but the pH limitation and iron sludge production problems remain unsolved. Herein, we proposed a self-fenton system based on N-doped carbon dots modified ZnIn2S4 (NCDs@ZnIn2S4) composites that exhibits effective degradation of antibiotics under neutral pH using low amounts of Fe2+. The decoration of ZnIn2S4 with NCDs significantly increased the surface area, visible light absorption, charge transfer ability and oxygen adsorption ability. NCDs@ZnIn2S4 composites exhibited a high H2O2 production rate (1528 μM g−1•h−1) under visible light, which was 1.9 and 5.3 times higher than ZnIn2S4 and NCDs, respectively. Meanwhile, the Fe2+/NCDs@ZnIn2S4 system with a low concentration of Fe2+(1 mg/L) could remove over 95% levofloxacin and oxytetracycline within 30 min. Interestingly, the highest degradation efficiency occurred under neutral pH. Quenching experiments and analytical measurements indicated that the high catalytic performance under pH = 7 with low amounts of Fe2+ stemmed from the higher amount of inner-generate H2O2 under neutral pH and easy regeneration of Fe2+ by photoinduced electrons for high •OH yields. Additionally, the Fe2+/NCDs@ZnIn2S4 system exhibited high degradation performance under different water matrix and ultrahigh degradation efficiency towards levofloxacin under real sunlight irradiation. The work shows the prospects of photocatalysis-self-Fenton systems for overcoming the pH limitation and the difficulty of iron sludge separation in the purification of effluents.

Sustainable Production of Biofuels from Biomass Feedstocks Using Modified Montmorillonite Catalysts.

The rampant exploitation of fossil fuels has led to the significant energy scarcity and environmental disruption, affecting the sound momentum of development and progress of human civilization. To build a closed-loop anthropogenic carbon cycle, development of biofuels employing sustainable biomass feedstocks stands at the forefront of advancing carbon neutrality, yet its widespread adoption is mainly hampered by the high production costs. Montmorillonite, however, has garnered considerable attention serving as an efficient heterogeneous catalyst of ideal economic feasibility for biofuel production, primarily due to its affordability, accessibility, stability, and excellent plasticity. Up to now, nevertheless, it has merely received finite concerns and interests in production of various biofuels using montmorillonite-based catalysts. There is no timely and comprehensive review that addresses this latest relevant progress. This review fills the gap by providing a systematically review and summary in controllable synthesis, performance enhancement, and applications related to different kinds of biofuels including biodiesel, biohydrogenated diesel, levulinate, γ-valerolactone, 5-ethoxymethylfurfural, gaseous biofuels (CO, H2), and cycloalkane, by using montmorillonite catalysts and its modified forms. Particularly, this review critically depicts the design strategies for montmorillonite, illustrates the relevant reaction mechanisms, and assesses their economic viability, realizing sustainable biofuels production via efficient biomass valorization.

Convolutional neural network as an efficient alternative to supervised learners for modelling plant diseases

The high productivity of the agricultural sector is crucial to the Global economy, as well as the national economy. For instance, India increased its nation's GDP by a factor of 17% to 18% in the past few decades. In India, the farming sector is the primary source of income. Numerous insects and illnesses have an impact on plant development, amount, and quality of products. Therefore, it is crucial to find the diseases early in the plant's development. Image processing is used to identify plant diseases and pests. Artificial intelligence, machine learning, and visual analysis have all been used in the past few decades to find and diagnose plant diseases. These computerized techniques are excellent for quickly monitoring vast acreage. In this study, we used the foliage of potato plants to identify diseased leaves affected by early blight, as well as late blight. A Comparison is thus laid between the Convolutional Neural Network (CNN) Model for this purpose, and the standard Supervised Learning Classifiers, like k – Nearest Neighbors, Support Vector Machine, AdaBoost, etc. The comparison is subjected not only towards the traditional Metrics, like Accuracy, Precision, etc. but is expanded to temporal domains as well, that is the time taken to compute the results. Also, the training, and testing split used while incorporating the model is another deciding factor in its performance. Several such training–testing splits are considered for such, and thereby, the convolutional Neural Network Model is found to be better in performance than the Supervised Machine Learners irrespective of the Splitting Ratio of the Training, and the Testing Data.

Response of Solanum tuberosum L. to drip irrigation and nitrogen application: productivity, nutrition composition, bioactive compounds, antioxidant activity

Sustainable crop cultivation is a crucial goal in modern agriculture, aiming to attain high productivity while conserving natural resources. This requires the implementation of rational cultivation techniques, with proper irrigation and fertilization practices playing a crucial role in ensuring plant well-being and providing natural protection against biotic and abiotic stresses. The health-promoting properties of crops are also significantly influenced by irrigation and fertilization. This study investigated the productivity, nutritional composition, bioactive compounds, and antioxidant capacity of Vineta early potato cultivar tubers under drip irrigation conditions, combined with nitrogen fertilization through fertigation or broadcasting. Two-factor trials included drip irrigation (control or drip) and nitrogen application (broadcast or fertigation). Precise treatments, such as drip irrigation and N-fertigation were found to enhance all productivity traits. Both practices positively impacted tuber nutrient content. The highest levels of total polyphenols and chlorogenic acid were observed in non-irrigated and broadcasted tubers. Drip-irrigated and N-fertigated tubers exhibited high levels of vitamin C and antioxidant activity. In summary, the combination of drip irrigation with appropriate fertilization methods positively influenced potato crops, as evidenced by an increase in productivity and the quality of tubers.

Optimizing Construction Spoil Reactivity for Cementitious Applications: Effects of Thermal Treatment and Alkaline Activation

Construction spoil (CS), a prevalent type of construction and demolition waste, is characterized by high production volumes and substantial stockpiles. It contaminates water, soil, and air, and it can also trigger natural disasters such as landslides and debris flows. With the advent of alkali activation technology, utilizing CS as a precursor for alkali-activated materials (AAMs) or supplementary cementitious materials (SCMs) presents a novel approach for managing this waste. Currently, the low reactivity of CS remains a significant constraint to its high-value-added resource utilization in the field of construction materials. Researchers have attempted various methods to enhance its reactivity, including grinding, calcination, and the addition of fluxing agents. However, there is no consensus on the optimal calcination temperature and alkali concentration, which significantly limits the large-scale application of CS. This study investigates the effects of the calcination temperature and alkali concentration on the mechanical properties of CS–cement mortar specimens and the ion dissolution performance of CS in alkali solutions. Mortar strength tests and ICP ion dissolution tests are conducted to quantitatively assess the reactivity of CS. The results indicate that, compared to uncalcined CS, the ion dissolution performance of calcined CS is significantly enhanced. The dissolution amounts of active aluminum, silicon, and calcium are increased by up to 420.06%, 195.81%, and 256.00%, respectively. The optimal calcination temperature for CS is determined to be 750 °C, and the most suitable alkali concentration is found to be 6 M. Furthermore, since the Al O bond is weaker and more easily broken than the Si O bond, the dissolution amount and release rate of active aluminum components in calcined CS are substantially higher than those of active silicon components. This finding indicates significant limitations in using CS solely as a precursor, emphasizing that an adequate supply of silicon and calcium sources is essential when preparing CS-dominated AAMs.

Applying double cropping and interactive irrigation in the North China Plain using WRF4.5

Abstract. Irrigated cultivation exerts a significant influence on the local climate and the hydrological cycle. The North China Plain (NCP) is known for its intricate agricultural system, marked by expansive cropland, high productivity, compact rotation, a semi-arid climate, and intensive irrigation practices. As a result, there has been considerable attention on the potential impact of this intensive irrigated agriculture on the local climate. However, studying the irrigation impact in this region has been challenging due to the lack of an accurate simulation of crop phenology and irrigation practices within the climate model. By incorporating double cropping with interactive irrigation, our study extends the capabilities of the Weather Research Forecast (WRF) model, which has previously demonstrated commendable performance in simulating single-cropping scenarios. This allows for two-way feedback between irrigated crops and climate, further enabling the inclusion of irrigation feedback from both ground and vegetation perspectives. The improved crop modeling system shows significant enhancement in capturing vegetation and irrigation patterns, which is evidenced by its ability to identify crop stages, estimate field biomass, predict crop yield, and project monthly leaf area index. The improved simulation of large-scale irrigated crops in the NCP can further enhance our understanding of the intricate relationship between agricultural development and climate change.

A Cost–Benefit Analysis for the Economic Evaluation of Ecosystem Services Lost Due to Erosion in a Mediterranean River Basin

Soil degradation in Europe is mostly represented by soil erosion that, as a sediment production mechanism, is the main environmental threat to many watersheds, including the Bovilla watershed (Tirana), useful for the supply of drinking water to the city, and therefore, the care of water quality is of particular interest. The soil erosion of the Bovilla watershed was monitored in a work set up in June 2017. Following this work, this research updates the previous data on soil loss at the Bovilla watershed in t/ha/year to September 2019 and focuses on the identification and monetary evaluation of the ecosystem services (ESs) linked to soil erosion (loss of carbon, loss of mineral elements, habitat quality, crop productivity, and sustainable tourism suitability). Then, we applied the replacement cost analysis to test the economic convenience and suggest the adoption of sustainable land management practices (e.g., reforestation) able to improve the quality water in this watershed. The study carried out demonstrates that the values of soil lost due to erosion vary depending on the type of land use (ranging from average values of 120.32 t/ha for bare land to values of 8.16 t/ha for wooded areas). Furthermore, from the application of monetary methods for the evaluation of some ecosystem services linked to erosion (loss of carbonaceous and mineral elements, habitat quality, productivity, suitability for sustainable tourism), it clearly emerges that the value of the productivity of agricultural crops varies from EUR 0 to 35,320.50/ha and that the service represents a more incisive service than the previous ones, so much so as to make the conversion of some agricultural land with high productivity values into wooded areas economically disadvantageous. The data from this study may help to develop Bovilla watershed management strategies for erosion and pollution control and sediment remediation mainly in agricultural lands. A program of measures can be effective for controlling soil erosion, but it must be implemented over long time frames, and it requires relevant investments from the public and private sectors, also with a view to increase the allocation of economic values of monetary compensation aimed at those who decide to start forestation projects on highly productive soils.

An overview of the constructions of conveyors for moving bulk materials, comparison and study of their parameters

The production of concrete mixes, along with their use in the production of building materials and structures, is one of the key processes in the construction industry during the construction, restoration and repair of buildings and structures. Because of this, the need to create modern concrete mixing plants that will meet the requirements of minimum energy consumption and maximum productivity of concrete mixture production is an urgent task. Not only the main operations, which include the dosing of the components of the mixture and their mixing, but also the maintenance operations, namely operations that ensure the timely movement of the components of the concrete mixture from warehouses to the main technological equipment, affect the set rhythm of the concrete mixture production. Conveyors of various types and designs are used to move bulk materials, such as crushed stone and sand. For the rational selection of such equipment in accordance with the characteristics of the cargo to be transported, knowledge of the types of conveyors, their structures and parameters, understanding of operation issues and methods of parameter calculation are required. In addition, it is worth paying attention to the following parameters: maximum cargo transportation productivity, low energy consumption per unit of moved products, low metal content of the structure. The work reviewed the most common designs of conveyors used to move bulk materials in concrete mixing plants, analyzed the disadvantages and advantages of conveyors, as well as technical parameters. As a result, the predominant directions for the use of belt and plate conveyors at construction enterprises were determined. The advantages of belt conveyors, which contribute to their widespread distribution, are high productivity, simplicity of design, reliability, quiet operation, low specific power consumption. When choosing a conveyor, it is recommended to choose the equipment with the highest productivity and the lowest power of the drive motors, however, the performance should be clearly related to other technological equipment.

Insights into Blue Whale (Balaenoptera musculus L.) Population Movements in the Galapagos Archipelago and Southeast Pacific

The Galapagos Marine Reserve is vital for cetaceans, serving as both a stopover and residency site. However, blue whales, occasionally sighted here, exhibit poorly understood migratory behavior within the Galapagos and the broader Eastern Tropical Pacific. This study, the first to satellite tag blue whales in the Galapagos (16 tagged between 2021 and 2023), explored their behavior in relation to environmental variables like chlorophyll-a concentration, sea surface temperature (SST), and productivity. Key findings show a strong correlation between foraging behavior, high chlorophyll-a levels, productivity, and lower SSTs, indicating a preference for food-rich areas. Additionally, there is a notable association with geomorphic features like ridges, which potentially enhance food abundance. Most tagged whales stayed near the Galapagos archipelago, with higher concentrations observed around Isabela Island, which is increasingly frequented by tourist vessels, posing heightened ship strike risks. Some whales ventured into Ecuador’s exclusive economic zone, while one migrated southward to Peru. The strong 2023 El Niño–Southern Oscillation event led to SST and primary production changes, likely impacting whale resource availability. Our study provides crucial insights into blue whale habitat utilization, informing adaptive management strategies to mitigate ship strike risks and address altered migration routes due to climate-driven environmental shifts.

A high magnetic energy product obtained in hot-deformed Nd-Fe-B magnet by chemical plating Fe-Co bilayer films

This study investigates a novel method, chemical plating, with the goal of integrating nano-sized ferromagnetic materials into hot-deformed Nd-Fe-B magnets, potentially enhancing their magnetic properties. Coating Fe-Co double layers on Nd-Fe-B magnetic powder resulted in a significant improvement in the remanence over 10 %, increasing from 1.37 to 1.45 T, as well as an increase in the maximum magnetic energy product from 45.95 to 52.02 MGOe in hot-deformed Nd-Fe-B magnets. Microstructure observations revealed that the coarse non-oriented grains at the interface of powder ribbons were suppressed and the texture of grain alignment in the interior of powder ribbons was enhanced after the coating of Fe-Co. Additionally, there was a noticeable increase in the concentrations of ferromagnetic elements in intergranular phases. The reduced coarse grain regions, the enhanced grain alignment and the increased ferromagnetic intergranular phases are considered to be the main reasons for the enhancement of remanence and maximum magnetic energy product in hot-deformed magnets.

Hydrogen evolution coupled with microplastic upcycling in seawater via solvent-free plasma F-functionalized CoNi alloy catalysts

The deleterious impacts of marine polyethylene terephthalate (PET) microplastic pollution on aquatic organisms have garnered global concern. Crafting a more cost-effective method to derive high value-added products from these microplastics is a pivotal challenge in marine resource reuse. This study introduces a strategy for electrooxidation of PET microplastics coupled with seawater electrolysis for green hydrogen production, resulting in value-added chemical formic acid at the anode and hydrogen gas at the cathode. Specifically, a catalyst composed of carbon nanotube-interconnected CoNi alloy-loaded carbon nanocages was fabricated, and F were doped onto the catalyst surface via solvent-free CF4 plasma treatment. Owing to its unique hollow 1D/3D interconnected structure, the synergistic effect of the CoNi alloy, and the ease of reconstruction of metal-F bonds in an alkaline environment, the prepared electrocatalyst exhibited excellent performance in the hydrogen evolution reaction and oxygen evolution reaction, the current density of 10 mA cm−2 was obtained at the overpotential of 187 mV and 315 mV, respectively. Furthermore, the proposed coupling strategy achieved a potential saving of 165 mV at a current density of 10 mA cm−2. This research offers promising prospects for the development of “marine hydrogen mining” and the management of ocean microplastics.

Mass Production of Multishell Hollow SiO2 Spheres With Adjustable Void Ratios and Pore Structures.

SiO2 multishell hollow spheres (MHSs) as supports have multiple porous layers and internal voids, which present notable advantages in regulating mass transport and chemical reactions. However, practical applications of SiO2 MHSs are severely hindered because of their high costs and low production efficiency issues. Herein, it is overcome these obstacles by developing a precursor hydrolysis method and demonstrate a cost-effective production of void-ratio tunable SiO2 MHSs on a large scale, which has a much lower cavitation temperature (25°C) and one order of magnitude decrease in cost. In addition, the new method can also be applied to fabricate TiO2 and SnO2 hollow spheres (HSs). In particular, an NH4Cl precipitation-pyrolysis strategy is developed to tune the pore diameters and pore distributions of SiO2 MHSs with different void ratios. SiO2 MHSs with varying void ratios and pore distributions have the broadest controlling release time ranges (30-430h). The precursor hydrolysis method and NH4Cl precipitation-pyrolysis strategy offer adequate stimulus to push forward SiO2 MHSs from laboratory-scale to industry-scale applications.

Valorization of Glycerol to Glycerol Carbonate and Glycidol by Different Dialkyl Carbonates Utilizing Tricalcium Aluminate Hexahydrate as Transesterification Catalyst

Herein, we report a base‐catalyzed transesterification reaction of glycerol, a waste product of the biodiesel industry, with various dialkyl carbonates, which act both as reactants and solvents, to convert glycerol carbonate into an industrially useful molecular building block. The catalyst, being used for the first time, is also a waste product from industry, present in bauxite residues and in the Portland cement, simply known as tricalcium aluminate. Despite being well‐known and readily available, this solid is only extremely poorly researched catalyst, nevertheless, using dimethyl and diethyl carbonate, glycerol conversion rates >80% and glycerol carbonate yields >60% could be achieved in just 1 hour (under air atmosphere, and reflux). In a comparison of the performance with other catalysts commonly researched today, as well as with other components of red mud and cements, the results showed that tricalcium aluminate is excellent, cheap and largely environmentally friendly material for this purpose. Reusability studies of the catalysts have also shown that they provide high conversion and product yields even after repeated use, although different material characterization techniques showed intense glycerol‐catalyst surface interaction and intermediate product formation, the deactivating side effects of which could be avoided by catalyst regeneration steps.

4-Octyl itaconate attenuates renal tubular injury in db/db mice by activating Nrf2 and promoting PGC-1α-mediated mitochondrial biogenesis

Objectives: The aim of this study was to investigate the mechanism of itaconate’s potential effect in diabetic kidney disease. Methods: Renal immune responsive gene 1 (IRG1) levels were measured in db/db mice and streptozotocin (STZ) + high-fat diet (HFD)-induced diabetic mice. Irg1 knockout mice were generated. db/db mice were treated with 4-octyl itaconate (4-OI, 50 mg/kg), a derivative of itaconate, for 4 weeks. Renal function and morphological changes were investigated. Ultrastructural alterations were determined by transmission electron microscopy. Results: Renal IRG1 levels were reduced in two diabetic models. STZ+HFD-treated Irg1 knockout mice exhibited aggravated renal tubular injury and worsened renal function. Treatment with 4-OI lowered urinary albumin-to-creatinine ratio and blood urea nitrogen levels, and restored renal histological changes in db/db mice. It improved mitochondrial damage, increased expressions of peroxisome-proliferator-activated receptor γ coactivator-1α (PGC-1α) and mitochondrial transcription factor A (TFAM) in the renal cortex of db/db mice. These were confirmed in vitro; 4-OI improved high glucose-induced abnormal mitochondrial morphology and TFAM expression in HK-2 cells, effects that were inhibited by PGC-1α silencing. Moreover, 4-OI reduced the number of apoptotic cells in the renal cortex of db/db mice. Further study showed that 4-OI increased renal Nrf2 expression and decreased oxidative stress levels in db/db mice. In HK-2 cells, 4-OI decreased high glucose-induced mitochondrial ROS production, which was reversed by Nrf2 silencing. Nrf2 depletion also inhibited 4-OI-mediated regulation of PGC-1α, TFAM, and mitochondrial apoptotic protein expressions. Conclusions: 4-OI attenuates renal tubular injury in db/db mice by activating Nrf2 and promoting PGC-1α-mediated mitochondrial biogenesis.

The Biological and Genetic Mechanisms of Fruit Drop in Apple Tree (Malus × domestica Borkh.)

The apple tree (Malus × domestica Borkh.) belongs to the Rosaceae. Due to its adaptability and tolerance to different soil and climatic conditions, it is cultivated worldwide for fresh consumption. The priorities of apple growers are high-quality fruits and stable yield for high production. About 90 to 95 percent of fruits should fall or be eliminated from apple trees to avoid overcropping and poor-quality fruits. Apple trees engage in a complex biological process known as yield self-regulation, which is influenced by several internal and external factors. Apple buds develop in different stages along the branches, and they can potentially give rise to new shoots, leaves, flowers, or fruit clusters. The apple genotype determines how many buds will develop into fruit-bearing structures and the capacity for yield self-regulation. Plant hormones such as ethylene, cytokinins, auxins, and gibberellins play a crucial role in regulating the fruit set, growth, and development, and the balance of these hormones influences the flowering intensity, fruit size, and fruit number on the apple tree. Apple growers often interfere in the self-regulation process by manually thinning fruit clusters. Different thinning methods, such as by hand, mechanical thinning, or applying chemical substances, are used for flower and fruit thinning. The most profitable in commercial orchards is the use of chemicals for elimination, but more environmentally sustainable solutions are required due to the European Green Deal. This review focuses on the biological factors and genetic mechanisms in apple yield self-regulation for a better understanding of the regulatory mechanism of fruitlet abscission for future breeding programs targeted at self-regulating yield apple varieties.

Systematic literature review of the humanistic and economic burden of focal epilepsy and primary generalized tonic-clonic seizures in adults.

This systematic literature review (SLR) assessed the humanistic and economic burden of focal epilepsy and primary generalized tonic-clonic seizures (PGTCS) in adults to evaluate these domains in both populations and identify evidence gaps to inform future research. A search was conducted on December 7, 2022, using MEDLINE and Embase to identify studies published from 2012 onwards reporting humanistic burden (patient-reported or caregiver-reported outcomes or utilities, qualitative evaluations), economic burden (productivity loss, caregiver and societal costs of epilepsy), and sleep-related outcomes. Of the 2830 citations identified, 136 were included. Most studies were in the focal epilepsy population; very few studies reported outcomes in the PGTCS population. The presence of epilepsy-specific instruments varied based on the domain evaluated. Epilepsy exerted considerable humanistic and economic burden. Indicators of poor disease control (e.g., high seizure frequency, resistance to anti-seizure medications, polypharmacy) increased epilepsy burden. Seizure frequency and type, disease severity, and polypharmacy also affected work productivity. Adults with epilepsy, particularly focal epilepsy, reported higher indirect costs, more sick days accrued, and early entry into retirement. Caregivers similarly reported high productivity loss and absenteeism related to caregiving duties. The results of this SLR highlight the high humanistic and economic burden of focal epilepsy and PGTCS, although limited data were available for the PGTCS population. The results include patient-reported outcome data specific to focal epilepsy and PGTCS, expanding the limited humanistic burden evidence identified in previous reviews, and show the effect of poor disease control on individuals' lives and as a driver of indirect costs. PLAIN LANGUAGE SUMMARY: Our systematic literature review identified studies that evaluated the impact of focal epilepsy and primary generalized tonic-clonic seizures on patients and their caregivers. We found that focal epilepsy negatively impacted patients' mental health and sleep and was associated with higher indirect costs and lower work productivity in people with more severe disease. The impact of primary generalized tonic-clonic seizures on patients was rarely reported, and future research is needed.

Design and Technological Aspects of Integrating Multi-Blade Machining and Surface Hardening on a Single Machine Base

Modern mechanical engineering faces high competition in global markets, which requires manufacturers of process equipment to significantly reduce production costs while ensuring high product quality and maximum productivity. Metalworking occupies a significant part of industrial production and consumes a significant share of the world’s energy and natural resources. Improving the technology of manufacturing parts with an emphasis on more efficient use of metalworking machines is necessary to maintain the competitiveness of the domestic machine tool industry. Hybrid metalworking systems based on the principles of multi-purpose integration eliminate the disadvantages of monotechnologies and increase efficiency by reducing time losses and intermediate operations. The purpose of this work is to develop and implement a hybrid machine tool system and an appropriate combined technology for manufacturing machine parts. Theory and methods. Studies of the possible structural composition and layout of hybrid equipment at integration of mechanical and surface-thermal processes were carried out, taking into account the basic provisions of structural synthesis and componentization of metalworking systems. Theoretical studies were carried out using the basic provisions of system analysis, geometric theory of surface formation, design of metalworking machines, methods of finite elements, and mathematical and computer modeling. The mathematical modeling of thermal fields and structural-phase transformations during HEH HFC was carried out in ANSYS (version 19.1) and SYSWELD (version 2010) software packages using numerical methods of solving differential equations of unsteady heat conduction (Fourier equation), carbon diffusion (2nd Fick’s law) and elastic–plastic behavior of the material. The verification of the modeling results was carried out using in situ experiments employing the following: optical and scanning microscopy; and mechanical and X-ray methods of residual stress determination. Formtracer SV-C4500 profilograph profilometer was used in the study for simultaneous measurement of shape deviations and surface roughness. Surface topography was assessed using a Walter UHL VMM 150 V instrumental microscope. The microhardness of the hardened surface layer of the parts was evaluated on a Wolpert Group 402MVD. Results and discussion. The original methodology of structural and kinematic analysis for pre-design studies of hybrid metalworking equipment is presented. Methodological recommendations for the modernization of multi-purpose metal-cutting machine tool are developed, the implementation of which will make it possible to implement high-energy heating with high-frequency currents (HEH HFC) on a standard machine tool system and provide the formation of knowledge-intensive technological equipment with extended functionality. The innovative moment of this work is the development of hybrid metalworking equipment with numerical control and writing a unique postprocessor to it, which allows to realize all functional possibilities of this machine system and the technology of combined processing as a whole. Special tooling and tools providing all the necessary requirements for the process of surface hardening of HEH HFC were designed and manufactured. The conducted complex of works and approbation of the technology of integrated processing in real conditions in comparison with traditional methods of construction of technological process of parts manufacturing allowed to obtain the following results: increase in the productivity of processing by 1.9 times; exclusion of possibility of scrap occurrence at finishing grinding; reduction in auxiliary and preparatory-tasking time; and reduction in inter-operational parts backlogs.