The global demand for blueberries has increased due to their health benefits, but postharvest losses, particularly firmness loss and decay, present significant challenges. This study evaluated the effects of high CO2 concentrations (15% and 20%) applied for 3 d at 1.0 °C on highbush (cv. 'Duke') and rabbiteye (cv. 'Ochlockonee') blueberries, with a focus on quality maintenance during cold storage. The quality parameters evaluated included titratable acidity, pH, total soluble solids, weight loss, and decay. The effect of gaseous treatments on firmness was analyzed using mechanical parameters and the expression of genes related to cell wall integrity (XTH23, PL8, PG, PM3, EXP4, and VcGH5). Treatment efficacy varied between species. High CO2 levels reduced decay in both cultivars, but only the highbush cultivar ('Duke') showed improvements in firmness. In 'Duke', CO2 treatments affected the expression of XTH23, PL8, and GH5, while the role of PG and PME in maintaining firmness was minimal, with no significant differences between treatments. In 'Ochlockonee', CO2 effectively reduced weight loss but did not improve firmness. In conclusion, these results highlight the need for tailored postharvest strategies for different blueberry cultivars and suggest that short-term high CO2 treatments may effectively prolong the postharvest life of highbush blueberries.
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