High Exposure Research Articles

Association between youth blood pressure and exposure to pediatric fruit and vegetable prescriptions.

Health impacts of pediatric fruit and vegetable prescription programs (FVPPs) are unclear. This study assessed whether exposure to an FVPP that provided $15 produce prescriptions during pediatric visits was associated with differences in child diet, food security, physical activity, weight status, and blood pressure. This cross-sectional analysis included data from caregiver-child dyads with varying levels of exposure to the FVPP. Dyads completed surveys at pediatric offices. Trained research assistants measured height and weight of children and recorded blood pressure through chart review. Causal inference analyses using propensity score adjustments compared outcomes of exposure groups. 680 dyads enrolled. Youth who received ≥1 prescription (exposed) reported greater physical activity compared to youth who received no prescriptions (unexposed). Blood pressure percentiles were lower among exposed when compared to unexposed youth (63.273 versus 75.060 for SBP; 71.472 versus 77.548 for DBP); and fewer exposed children recorded elevated blood pressure when compared to unexposed (0.141 versus 0.343 for SBP; 0.199 versus 0.344 for DBP; and 0.286 versus 0.531 overall). Similar findings were obtained using duration as a measure of exposure. Youth exposed to the FVPP experienced greater physical activity and healthier blood pressure. Findings may indicate novel health-promoting effects of pediatric FVPPs. When compared to youth with no exposure, youth exposed to a pediatric fruit and vegetable prescription program recorded greater physical activity and healthier blood pressure. Youth with high exposure (≥24 months) to the fruit and vegetable prescription program experienced greater physical activity and healthier blood pressure when compared to youth with low exposure (<24 months). This extends evaluation of pediatric fruit and vegetable prescription programs beyond feasibility and preliminary effectiveness to indicate potential of such programs in positively influencing physical activity and blood pressure of participating youth. Findings indicate novel health-promoting effects of pediatric fruit and vegetable prescription programs.

Conservation Under Siege: The Intersection of Tourism and Environmental Threats in Croatian Protected Areas

This paper investigates the illegal activities that may negatively impact the environment in Croatia’s protected areas, particularly those associated with tourism. Failure Mode and Effects Analysis (FMEA) and sensitivity analysis are used to identify distinct high-risk activities specific to different park types. Coastal parks are found to be particularly vulnerable to maritime-related illegal activities, with high risk exposure from mooring vessels and swimming in prohibited areas. These parks also face significant risks from illegal fishing and the capture of strictly protected plants and animals, highlighting the need for enhanced marine ecosystem protection. Mountainous and forest parks face significant risks from fire-related activities, while wetland parks are sensitive to poaching and illegal fishing. Additionally, diverse landscape parks show increased vulnerability to littering, poaching, and the collection or harvesting of strictly protected plants and animals. The results underscore the necessity for tailored risk-management strategies that prioritize interventions based on park-specific challenges. By addressing the unique vulnerabilities of each park type, this study contributes insights into mitigating environmental threats posed by tourism and emphasizes the importance of continuous monitoring and adaptive management in protected areas.

Health issues and management practices of pesticide exposure in Ethiopia, East Africa

Pesticides are commonly used in agriculture for pest control, but their improper usage and handling can lead to high farmer exposures, negative health effects, and pollution of water bodies. The purpose of this study was to investigate farmer’s perceptions of the effects of pesticide runoff on water bodies, and to describe their self-reported knowledge of appropriate pesticide use, methods of application, sources of information, and related health issues and management strategies in Ethiopia. Data were collected via structured questionnaire, which was administered as an in-person interview to 582 farmers who cultivated vegetables. To assess unsafe pesticide handling practices, researchers observed the storage of pesticides and the condition of personal protective equipment and asked farmers about their pesticide disposal methods. The study found that nearly 87.97% of farmers reported mixing pesticides using a stick or their bare hands. Most respondents (88.83%) mixed pesticides to spray just on their farmland, and some (10.14%) were near water sources. It revealed that farmers have a low level of awareness about the risks and effects associated with pesticides and do not follow safe practices. Out of the farmers who had utilized pesticides, 55.67% reported encountering health issues associated with pesticide exposure. To reduce exposure and health risks, the study suggests implementing comprehensive interventions, such as integrated pest management, safety training, and alternative control methods.

Sustainable lightweight concrete with EPS beads and cork additives: a study on physico-mechanical properties of eco-friendly composites

This study investigates the properties of lightweight concrete composites incorporating expanded polystyrene (EPS) and cork as aggregates, focusing on their effects on density, thermal performance, and mechanical strength. The results show that using EPS and cork significantly reduces the density of the composites, making them suitable for applications where weight reduction is critical, such as in energy-efficient building materials. Additionally, the thermal conductivity of these composites decreases substantially, enhancing their insulating properties. However, the reduction in density is coupled with a significant decrease in compressive strength, with values up to 94% lower than conventional concrete. This limits the use of these lightweight composite materials to non-structural applications or situations where low load-bearing capacity is acceptable. The study also reveals an increase in porosity and capillary water absorption in the composites, which may compromise their durability, particularly in environments with high moisture exposure. In conclusion, lightweight concrete composites incorporating EPS and cork offer considerable advantages in terms of reduced weight and improved thermal insulation. However, for structural applications or where higher mechanical performance is required, adjustments such as the inclusion of reinforcing materials or reducing the lightweight aggregate content may be needed to ensure optimal performance and durability.

Semi-mechanistic population pharmacokinetic modeling of DZIF-10c, a neutralizing antibody against SARS-Cov-2: predicting systemic and lung exposure following inhaled and intravenous administration

DZIF-10c (BI 767551) is a recombinant human monoclonal antibody of the IgG1 kappa isotype. It acts as a SARS-CoV-2 neutralizing antibody. DZIF-10c has been developed for both systemic exposure by intravenous infusion as well as for specific exposure to the respiratory tract by application as an inhaled aerosol generated by a nebulizer. An integrated preclinical/clinical semi-mechanistic population pharmacokinetic model was developed to characterize the exposure profile of DZIF-10c in the systemic circulation and lungs. To inform and reduce uncertainty around exposure in the lungs following different methods of dosing, preclinical cynomolgus monkey data was combined with human data using allometric scaling principles. Human serum concentrations of DZIF-10c from two clinical trials were combined with serum/plasma and lung epithelial lining fluid (ELF) concentrations from three preclinical studies to characterize the relationship between dosing, serum/plasma, and lung exposure. The final model was used to predict exposure in the lungs following different routes of administration. Simulations showed that inhalation provides immediate and relevant exposure in the lung ELF at a much lower dose compared with an infusion. Combining inhalation with intravenous therapy results in high and sustained DZIF-10c exposure in the lungs and systemic circulation, thereby combining the benefits of both routes of administration. By combining preclinical data with clinical data (via allometric scaling principles), the developed population pharmacokinetic model reduced uncertainty around exposure in the lungs allowing evaluation of alternative dosing strategies to achieve the desired concentrations of DZIF-10c in human lungs.

An evolutionary game study of traffic safety risk regulation considering public participation behavior

With the development of transportation technology, the improvement of transportation efficiency also increases the complexity and diversity of traffic safety risks; the public’s concern for traffic safety risks is increasing, and the traditional regulatory model is difficult to meet the improvement of the effectiveness of the safety regulation of the huge transportation network and the public’s changing safety needs. In order to enhance the effectiveness of safety regulation, a game model of safety risk regulation among the public, transport enterprises, and regulators is constructed to simulate the effects of different traffic risks, penalty levels, and exposure rates on the behavior of transport enterprises and regulators. The results of the study show that: traffic risk has a positive facilitating effect on the participation of transport enterprises and the public in the regulation of safety risks, which will accelerate the process of stabilization of their strategies; transport enterprises have a high sensitivity to changes in the level of punishment, and only when the level of punishment exceeds a certain threshold will they be able to promote the choice of complying with the transport; and a higher exposure will lead to a greater tendency for transport enterprises to choose complying with the transport.

Temporal changes of neurobehavior in rats following varied blast magnitudes and screening of serum biomarkers in early stage of brain injury

Blast neurotrauma has been linked to impairments in higher-order cognitive functions, including memory, attention, and mood. Current literature is limited to a single overpressure exposure or repeated exposures at the same level of overpressure. In this study, a rodent model of primary blast neurotrauma was employed to determine the pressure at which acute and chronic neurological alterations occurred. Three pressure magnitudes (low, moderate and high) were used to evaluate injury thresholds. A biology shock tube (BST) was used to simulate shock waves with overpressures of 60 kPa, 90 kPa and 120 kPa respectively. Neurological behavior of the rats was assessed by the Multi-Conditioning System (MCS) at 1 d, 7 d, 28 d and 90 d after shock wave exposure. Serum dopamine (DA), 5-hydroxytryptamine (5-HT), brain-derived neurotrophic factor (BDNF) and gamma-aminobutyric acid (GABA) were measured at the same time points. The proteomic analysis was conducted to identify potentially vulnerable cellular and molecule targets of serum in the immediate post-exposure period. Results revealed that: (1) Anxiety-like behavior increased significantly at 1 d post-exposure in the medium and high overpressure (90 kPa, 120 kPa) groups, returned to baseline at 7 days, and anxiety-like behavior in the high overpressure groups re-emerged at 28 d and 90 d. (2) High overpressure (120 kPa) impaired learning and memory in the immediate post-exposure period. (3) The serum DA levels decreased significantly at 1 d post-exposure in the medium and high overpressure groups; The 5-HT levels decreased significantly at 1 d and 90 d in the high overpressure groups; The BDNF levels decreased significantly at 90 d in the high overpressure groups. (4) Proteomic analysis identified 38, 306, and 57 differentially expressed proteins in serum following low, medium and high overpressure exposures, respectively. Two co-expressed proteins were validated. Functional analysis revealed significant enrichment of 1121, 2096, and 1121 Gene Ontology (GO) items and 33, 47, and 26 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, indicating extensive molecular responses to overpressure in the early phase. These findings suggest that exposure, even at moderate levels, can induce persistent neurobehavioral and molecular alterations, highlighting the need for further research into the long-term consequences of blast neurotrauma.

Evaluation of the potential of bryophytes to support seedlings development on waste rocks: a mesocosm study.

Revegetating waste rocks is essential for re-establishing a functional ecosystem, but it poses significant challenges. Nutrient-poor soil and high exposure to elements cause high seedling mortality in the first year. Survival can be increased by amending soil with organic matter before plantation to improve water retention and nutrient availability. However, this method comes with high environmental and economic costs. Using mesocosms, we tested the use of bryophytes as an alternative to improve seedling survival due to their ability to create a beneficial microclimate with improved soil humidity and increased nutrient availability, especially through biological nitrogen fixation. Two bryophyte species (Racomitrium canescens (Hedw.) Brid., an early succession, and Pleurozium schreberi (Brid.) Mitt., a late succession species) were evaluated with three higher plant species (Pinus banksiana, Rhododendron groenlandicum, and Populus balsamifera) planted in waste rocks. We further evaluated two distinct techniques for the application of bryophytes on waste rocks: evenly spreading fragments on the soil surface and regrouping the fragments around the higher plant stem. Results showed that R. canescens fixed significantly more nitrogen than P. schreberi on waste rock. On the contrary, P. schreberi released more nitrogen into the soil than R. canescens, likely due to higher mortality and nutrient loss during drying/rewetting cycles. While the short-term impact of bryophytes on seedlings was limited in this 6-month experiment, results suggest that bryophytes have the potential to support higher plants in the challenging conditions of waste rock revegetation by increasing nitrogen supply to seedlings.

Buprenorphine Salivary Gland Accumulation Sustaining High Oral Fluid Exposure and Increasing the Risk of Streptococcus mutans Biofilm Formation.

The US Food and Drug Administration (FDA) issued a warning about buprenorphine-induced dental caries of unknown mechanism in 2022. To investigate the potential mechanism, the association between local buprenorphine exposure and dental biofilm formation will be explored in this study. Female F344 rats were dosed with sublingual buprenorphine film or intravenous injection to explore the oral cavity exposure of the buprenorphine. The buprenorphine distribution in salivary glands after the sublingual and intravenous administration was also evaluated. To investigate the effects of buprenorphine exposure on dental caries formation, buprenorphine's impact on the biofilm formation of S. mutans in vitro was measured. The absolute sublingual bioavailability of buprenorphine in rats was 17.8% with a high ratio of oral fluid exposure to blood concentration in the pharmacokinetic study. Salivary gland concentrations of buprenorphine and its active metabolite norbuprenorphine were significantly higher than their blood concentrations after both sublingual (s.l.) and intravenous (i.v.) administration. Correlation analysis showed that the oral fluid concentration of buprenorphine and norbuprenorphine was highly correlated to salivary gland concentration rather than blood concentration. These data indicate that the salivary gland serves as an accumulation organ for buprenorphine, allowing prolonged oral fluid exposure to buprenorphine. Lastly, buprenorphine and its metabolites contributed to the biofilm formation of S. mutans in high concentration. Sublingual administration substantially increased the salivary gland distribution of buprenorphine and norbuprenorphine. Depot effects following sublingual dosing and salivary gland accumulation likely sustained high oral fluid exposure to buprenorphine and stimulated the biofilm formation of S. mutans.

Evaluation of Environmental Radiation Exposure and Algorithms for Determining the Occupational Effective Dose During 99mTc-MDP Bone Scintigraphy: A Comprehensive Analysis

This study evaluated the occupational exposure of radiopharmacists, nurses, radiological technologists, and radiological technologist assistants involved in 99mTc-MDP bone scintigraphy procedures. Actual occupational effective doses for individual staff needed ascertaining. An environmental radiation exposure audit revealed all nuclear medicine areas were compliant with ICRP and IAEA guidelines. To ascertain individual doses, they were recorded by OSL badges. The highest exposure was to the radiopharmacists, Hp(0.07) 2.19 µSv during radiolabeling. The nurse administering 99mTc-MDP recorded a dose of 0.27 µSv at Hp(0.07), 26.01% of the total occupational effective dose. The radiological technologist and assistant receive 73.05% of the total effective dose. However, the highest effective dose was received by the technologist assistant positioning patients for SPECT/CT scans, with an effective dose of 32.03 µSv. Single and double dosimetry effective dose estimate algorithms were evaluated, resulting in the double dosimetry being more accurate. The Padovani et al. algorithm was found to most closely align with 99mTc-MDP actual effective dose values (p &gt; 0.05), thereby validating the measurement methods used in this study. The research offers benchmark environmental exposure and effective doses applicable in audits and the continuous effort to enhance radiation safety for personnel during 99mTc-MDP bone scintigraphy.

Dose-response effects of multiple Ascaris suum exposures and their impact on lung protection during larval ascariasis

Background Human ascariasis is the most prevalent geohelminthiasis worldwide, affecting approximately 446 million individuals. In regions with endemic prevalence, the majority of infected adults are frequently exposed to the parasite and tend to have a low parasite load. Further studies are necessary to provide more evidence on the dynamics of infection and to elucidate the possible mechanisms involved in regulating protection, especially during the acute phase, also known as larval ascariasis. The aim of this study is to compare the impact of lung function between single and multiple infections in a murine model. Methods We infected BALB/c mice considering the frequency of exposures: single-exposure—SI; twice-exposures—RE 2x and thrice-exposures—RE 3x, and considering the doses of infection: 25 eggs—RE 25; 250 eggs—RE 250 and 2,500 eggs—RE 2500, followed by infection challenge with 2,500 eggs. From this, we evaluated: parasite burden in lungs, cellular and humoral response, histopathological and physiological alterations in lungs. Results The main results showed a reduction of parasite burden in the reinfected groups compared to the single-infected group, with protection increasing with higher exposure and dose. Furthermore, the RE 250 group exhibited a decrease of parasite burden close to RE 2500, but with less tissue damage, displaying the most favorable prognosis among the reinfected groups. Conclusion Our research indicates a dose-dependent relationship between antibody production and the intensity of the immune response required to regulate the parasite burden.

Symptomatic Improvement in Adults and Adolescents with Eosinophilic Esophagitis Requires Higher Systemic Dupilumab Exposure than Histologic Response.

We assessed potential mechanisms behind the requirement for more frequent dupilumab dosing in eosinophilic esophagitis (EoE) compared with other approved indications. Results for the phase 3 LIBERTY EoE TREET study co-primary endpoints (proportion of patients achieving a peak intraepithelial eosinophil count of ≤6 eosinophils per high-power field and absolute change from baseline in Dysphagia Symptom Questionnaire total score) were pooled in exposure-response analyses. A steep initial relationship then plateau was observed between higher dupilumab steady state trough concentrations (Ctrough) and decreased eosinophilic infiltration at Week 24, while a graded exposure-response relationship was observed for symptomatic improvement at Week 24. Patients with the highest exposures were more likely to achieve greater symptomatic benefit, independent of strictures or history of dilation. The dupilumab 300 mg qw regimen approved for adults and adolescents with EoE weighing ≥40 kg is supported by dose- and exposure-response relationships.

Platelet-Derived Growth Factor Subunit A Strengthens the Neurovascular Unit and Inhibits Retinal Vascular Regression Under Hyperoxic Conditions

Retinopathy of prematurity (ROP) is primarily caused by the exposure of preterm infants with underdeveloped blood vessels to high oxygen concentrations. This damages the astrocytes that promote normal vascular development, leading to avascularity, pathological neovascularization, and retinal detachment, and even blindness as the disease progresses. In this study, the aim was to investigate the differences in the characteristics of astrocytes and blood vessels between wild-type (WT) and genetically modified mice overexpressing platelet-derived growth factor subunit A (PDGF-A) in the retina immediately after high oxygen exposure, a protocol in the oxygen-induced retinopathy (OIR) model of ROP. Our results showed that PDGF-A mice exhibited an increased population of astrocytes and higher vascular density than WT mice and that PDGF-A strengthened the resistance to hyperoxic conditions. In the OIR model, PDGF-A mice had reduced avascular zone areas following hyperoxia exposure. Furthermore, immunostaining for NG2 and CD31 showed that pericytes tended to regress earlier than endothelial cells, particularly at the vessel edges in both WT and transgenic mice, indicating relatively higher susceptibility to hyperoxia-induced damage. These findings suggest that PDGF-A plays a crucial role in stabilizing retinal vessels and may serve as a novel therapeutic target for ROP, highlighting the potential significance of PDGF-A in the pathological mechanisms of retinal diseases.

Impaired brainstem auditory evoked potentials after in utero exposure to high dose paracetamol exposure

Paracetamol is an analgesic and antipyretic medication regarded as the safest over-the-counter pain and fever relief option during pregnancy. Paracetamol and its metabolites are known to reach the developing fetus through direct placental transfer and can cross the blood brain barrier. Several recent, large-scale epidemiologic studies suggest that in utero paracetamol exposure can increase the risk of neurodevelopmental conditions, including autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD) and developmental delay (DD). Since auditory processing deficits are a common feature of ASD, we hypothesized that animals exposed to paracetamol in utero will have impaired auditory brainstem function. We investigated this hypothesis by recording and analyzing click-evoked auditory brainstem responses (ABR) at postnatal day 21 and 29 in Sprague-Dawley rats. In utero exposure to high dose paracetamol exposure had no impact on body or brain weight. However, high dose paracetamol exposure did significantly delay ear opening and resulted in elevated ABR thresholds, and longer wave and interwave latencies. These changes in wave latency extended to the highest click intensity tested but were most severe near threshold. This data suggests that development and function of the auditory brainstem may be impacted by high dose paracetamol exposure and that simple, non-invasive tests of auditory function have utility as an early screening tool for neurodevelopmental disorders.

Favipiravir pharmacokinetics in Thai adults with mild COVID-19: A sub-study of interpatient variability and ethnic differences in exposure.

This sub-study sought to characterize the pharmacokinetics (PK) of favipiravir (FPV) within Thai adults and quantitatively assess differences in exposure to those previously reported in other populations as a basis to understand putative differences in efficacy between studies conducted in different regions. It was nested within a prospective trial of adults with symptomatic COVID-19 infection without pneumonia receiving 1800 mg FPV twice-daily on day 1 and 800 mg twice-daily thereafter. Individual PK profiles were fitted with a one-compartment disposition model (first-order absorption). Eight adults (seven female) with a median age of 39 years and BMI of 27.9 kg/m2 were included. Seven adults achieved plasma concentrations above the EC90 invitro target (25 mg/L), with minimum-maximum concentrations decreasing with repeat dosing. The mean FPV apparent clearance observed in this study was 1.1 L/h (coefficient of variation [CV]: 60%), apparent volume of distribution 20.6 L (CV: 40%), absorption rate constant 6.1 h (CV: 100%), and 2.4 daily % change in apparent clearance (CV: 315%). Higher exposures were observed in these Thai adults compared with data from previous studies in Chinese, Japanese, and Turkish populations, respectively. Current FPV doses recommended in Thailand achieved target plasma concentrations with higher exposures than those described previously in other populations. The limited sample size prohibits firm conclusions from being drawn but the presented data warrants confirmation with a view to interrogate the appropriateness of doses used in randomized clinical trials that failed to demonstrate efficacy.

Examining the impact of Anticholinergic Burden on Hospitalized Older People Receiving Concomitant Cholinesterase inhibitors.

Background: Cholinesterase (ChE) inhibitors enhance central cholinergic function and are considered as standard treatments to ameliorate symptoms relating to Alzheimer's disease. Though anticholinergic medications directly antagonize the effects of ChE inhibitors, they are commonly prescribed among hospitalized adults. Objective: To determine the impact of high anticholinergic burden (ACB) on length of stay (LOS) and 30-day readmission rates among hospitalized patients receiving concomitant ChE inhibitors. Methods: This was a retrospective cohort study conducted at a tertiary care academic medical center involving hospitalized patients on medical floors who received any Food and Drug Administration-approved ChE inhibitors during their hospital stay from October 1, 2022, to September 30, 2023. The primary outcome of the study was to compare hospital LOS among patients with high (ACB ≥ 3) versus low (ACB < 3) ACB. The secondary outcome was to assess the impact of ACB burden on 30-day readmission rates. Results: Among hospitalized adults, patients with high ACB exposure had a significantly longer hospital LOS (median: 5.50 vs 4.25 days; P < 0.001) than patients with low ACB exposure, after adjusting for covariates. Analysis of secondary outcome revealed that though the high ACB group had a higher 30-day readmission rate compared with the lower ACB group (6.8% vs. 2.2%), the difference was not statistically significant (OR = 3.46, 95% CI 0.85-14.08; P = 0.083). Conclusion: A high ACB exposure among older individuals taking concurrent ChE inhibitors is associated with a longer hospital stay.

Constrained Precipitation Extremes Reveal Unequal Future Socioeconomic Exposure

AbstractExtreme precipitation can lead to major flooding, impacting human health and safety. Thus, reliable projections of population and GDP exposure to future extreme precipitation are imperative. Here, we quantify future precipitation characteristics from robust emergent constraint relationships between historical and future monthly precipitation extremes (99th percentile) across 19 CMIP6 models (r2 &gt; 0.7 in 74–84% of 0.5° global land grids), and narrow uncertainty by 37.0–39.5% (absolute reduction being 0.753–0.774 mm/day). The constrained grid‐averaged future 99th percentile extreme is 6.96 ± 0.0059, 7.03 ± 0.0061, 7.11 ± 0.0063, and 7.29 ± 0.0067 mm/day, under SSP126, SSP245, SSP370, and SSP585, respectively, which exceeds historical extremes substantially in terms of intensity (12.9–19.7%) and frequency (1.6–2.3 times more). Future population and GDP exposed to 99th percentile extreme precipitation grow quickly, and are projected to exceed 1 million people in 27–40 countries and 10 billion USD (2005 Purchasing‐Power Parity) in 48–77 countries. Growth of future population exposure is dominated by an increase in extreme precipitation frequency rather than a rise in population, especially in developed countries. GDP exposure is controlled by the coupled effects of rapid socio‐economic development and significant shifts in precipitation frequency. Using indices of socio‐economic vulnerability, government effectiveness and economic freedom, we identify the unequal situation that high‐risk countries with high exposure are commonly characterized by low GDP per capita and high sociopolitical instability.

PM exposure during pregnancy affects childhood asthma via placental epigenetic changes: neuronal differentiation and proliferation and Notch signaling pathways

Particulate matter (PM) exposure during pregnancy increases the risk of developing asthma in children. However, the placental mechanisms have yet to be elucidated. This study aims to evaluate the mechanisms associated with PM exposure during pregnancy and asthma susceptibility via placental epigenetic dysregulation. We analyzed data from two independent Korean birth cohorts (COCOA, 684 children; PSKC, 818 children). Physician-diagnosed current asthma and bronchial hyperresponsiveness (BHR) via methacholine challenge tests were evaluated at age seven. We estimated PM exposure with a diameter < 10 μm (PM10) during pregnancy using land-use regression models. We performed genome-wide methylation profiling in the placenta of 40 samples in the COCOA study and analyzed the gene expression levels. High PM10 exposure during pregnancy increased the risk of developing current asthma and BHR in the COCOA study (aOR 2.36, 95% CI 1.06–5.22; aOR 2.14, 95% CI 1.40–3.27, respectively) and current asthma in the PSKC (aOR 2.62, 95% CI 1.35–5.09). The genes involved in neuronal differentiation and proliferation and Notch signaling pathways were significantly hypermethylated in children with high PM10-exposed asthma. The methylation and expression levels of eight genes (PAX6, REST, OLIG2, GLI1, ZBTB7A, NOTCH4, NOTCH1, and NOTCH3) in these pathways correlated with clinical parameters. This may effectively predict PM-related asthma through a prediction model using degrees of gene-based or CpG-based methylation (AUC = 0.96 and 0.93, respectively). PM10 exposure during pregnancy impacts asthma development in offspring via placental DNA hypermethylation via neuronal differentiation and proliferation and Notch signaling pathways.

The association between outdoor artificial light at night exposure and antenatal depression and anxiety symptoms: A retrospective cohort study in China

BackgroundOutdoor artificial light at night (ALAN) has emerged as a significant source of environmental pollution, however its association with antenatal depression and anxiety symptoms has not been explored before. MethodsThis study was based on a cohort study conducted at the Maternal and Child Health Care Center in Ma’anshan City, Anhui Province, China, which ultimately included 1047 pregnant women. Depression and anxiety symptoms were evaluated utilizing the self-administered Patient Health Questionnaire (PHQ-9) and the 7-item Generalized Anxiety Scale (GAD-7), respectively. Exposure levels to outdoor ALAN were calculated utilizing satellite data and the participants’ usual addresses. Logistic regression and restricted cubic spline were used to assess the association between exposure to outdoor ALAN and depression and anxiety symptoms in pregnant women. ResultsAfter adjusting for confounding factors, high ALAN exposure during the pre-pregnancy period (ORdepression = 3.16, 95% CI: 1.14∼8.75; ORanxiety = 3.09, 95% CI: 1.51∼6.28) and first trimester (ORdepression = 2.90, 95% CI: 1.13∼7.45; ORanxiety = 3.11, 95% CI: 1.55∼6.25) were associated with increased risks of antenatal depression and anxiety symptoms. Restricted cubic spline analyses showed the above associations were not nonlinear. ConclusionOur study is the first to propose that exposure to high levels of outdoor ALAN three months before pregnancy and during the first trimester of pregnancy is a risk factor for antenatal depression and anxiety symptoms.

Dietary exposure to high-intensity sweeteners by the Brazilian self-declared diabetic population and general Brazilian population

Due to an increasing use of high-intensity sweeteners in foods and potential health implications of high levels exposure, the intake of high-intensity sweeteners present in the diet of the Brazilian population and the self-declared diabetic population, based on national consumption data from the 2017-2018 Family Budget Survey (POF), was estimated. The occurrence of the high-intensity sweeteners was established by labeling foods obtained in physical stores and online, in two scenarios: average and maximum concentration. Food consumption data were derived for the self-declared diabetic Brazilian population and the Brazilian population for average consumers and consumers only, stratified by: sex, age, habitation, and regions from Brazil. For consumers only of the general Brazilian population, the intake of sodium cyclamate, steviol glycosides, and sucralose exceeded the Acceptable Daily Intake (ADI), mainly in adolescents (131.3%, 117.2, 123.0% of respective ADI). The estimated intake for the diabetic population was below ADI in all scenarios calculated. The most exposed diabetic population to sodium cyclamate and sucralose were female (69.6% and 59.4% of respective ADI) and the elderly (79.2% and 70.1% of respective ADI). The Brazilian population, mainly high consumers, was more exposed to high-intensity sweeteners than the self-declared diabetic population.