Higher Animal Welfare Standards Research Articles

A reflexion on the oxidative stress and animal welfare: a review.

A wide range of studies have documented the role of oxidative stress in the development of chronic pathological disorders and even in the aging itself. However, its significance to modern animal health and welfare remains neglected. Oxidative stress in biological systems refers to a disturbance in the balance between pro-oxidants and antioxidants in favour of the former, leading to potential damage of biomolecules. In farm animals, oxidative stress may be involved in several pathological conditions, including those that are relevant for animal production and the general welfare of the individuals, resulting in some cases in irreversible losses. The oxidative stress concept and how it may result in disease or be prevented are complex questions with no simple answers and therefore, call professionals for deep reflection, to maintain a high standard of animal welfare and production. The aim of this review was to gather relevant information on the characteristics of pro-oxidants and antioxidant as well as their significance in animal production systems.

GFI-YOLOv8: Sika Deer Posture Recognition Target Detection Method Based on YOLOv8.

As the sika deer breeding industry flourishes on a large scale, accurately assessing the health of these animals is of paramount importance. Implementing posture recognition through target detection serves as a vital method for monitoring the well-being of sika deer. This approach allows for a more nuanced understanding of their physical condition, ensuring the industry can maintain high standards of animal welfare and productivity. In order to achieve remote monitoring of sika deer without interfering with the natural behavior of the animals, and to enhance animal welfare, this paper proposes a sika deer individual posture recognition detection algorithm GFI-YOLOv8 based on YOLOv8. Firstly, this paper proposes to add the iAFF iterative attention feature fusion module to the C2f of the backbone network module, replace the original SPPF module with AIFI module, and use the attention mechanism to adjust the feature channel adaptively. This aims to enhance granularity, improve the model's recognition, and enhance understanding of sika deer behavior in complex scenes. Secondly, a novel convolutional neural network module is introduced to improve the efficiency and accuracy of feature extraction, while preserving the model's depth and diversity. In addition, a new attention mechanism module is proposed to expand the receptive field and simplify the model. Furthermore, a new pyramid network and an optimized detection head module are presented to improve the recognition and interpretation of sika deer postures in intricate environments. The experimental results demonstrate that the model achieves 91.6% accuracy in recognizing the posture of sika deer, with a 6% improvement in accuracy and a 4.6% increase in mAP50 compared to YOLOv8n. Compared to other models in the YOLO series, such as YOLOv5n, YOLOv7-tiny, YOLOv8n, YOLOv8s, YOLOv9, and YOLOv10, this model exhibits higher accuracy, and improved mAP50 and mAP50-95 values. The overall performance is commendable, meeting the requirements for accurate and rapid identification of the posture of sika deer. This model proves beneficial for the precise and real-time monitoring of sika deer posture in complex breeding environments and under all-weather conditions.

The Royal Zoological Society of Scotland's Approach to Assessing and Promoting Animal Welfare in Collaboration with Universities.

Good zoos have four aims-to conserve species, educate the public, engage in research, and provide recreation-all of which can only be achieved when underpinned by high animal welfare standards. In this paper, we share the approach that The Royal Zoological Society of Scotland's (RZSS) Edinburgh Zoo and Highland Wildlife Park take to animal welfare. We highlight the role that animal welfare research, in collaboration with universities, has had in enabling the zoo to take an evidence-based approach to welfare and to put findings into practice. We share the collaborative process through which we developed and piloted the current animal welfare assessment tools, how they were validated, and how they were tested for reliability as part of a long-term collaboration between the Royal Zoological Society of Scotland and the University of Stirling: (1) the RZSS Welfare Assessment Tool, a 50-question animal welfare assessment adapted from the British and Irish Association of Zoos and Aquariums (BIAZA) Toolkit; and (2) the Stirling Toolkit, a package of evidence-based resources for behavioural-data collection. Our aim is to facilitate standardised, evidence-based approaches to assessing animal welfare which, when finalised, can be used collaboratively across zoos.

Impact of Nutritional Strategies to Prevent Post-Weaning Diarrhoea on Performance, Behaviour, and Microbiota in Piglets from Organic Farming.

Organic livestock farming is committed to high environmental and animal welfare standards, although pathologies such as post-weaning diarrhoea (PWD) may appear. The main objective of this study was to assess nutritional strategies to prevent PWD in organic piglets. A total of 134 weaned piglets were fed one of three diets: high crude protein (17.8%, HCP), low crude protein (16.8%, LCP), and low crude protein supplemented with liquid whey (LCP+W). Piglets were assessed weekly for four weeks on the following parameters: diarrhoea incidence, additional health parameters, average daily gain, and behaviour. Faecal samples were taken to analyse the intestinal microbiota composition. Data were analysed using LMM and GLMM models and Shannon and Whittaker indexes. No significant effect of diet on diarrhoea incidence was found, but the LCP+W diet increased average daily gain. Pigs fed the LCP+W diet presented a lower percentage of drinking and negative social behaviour compared with the HCP diet, and LCP pigs presented higher exploration compared with HCP. In addition, LCP+W piglets showed a higher abundance of the beneficial genus Frisingicoccus. Although liquid whey did not reduce diarrhoea incidence, the benefits found in growth, microbiota composition, and reduced negative social behaviour indicate that it could be an optimal supplement to organic diets.

Health of Polo Horses.

This literature review analyzes the historical development of polo, its organizational structure, the course of the game, as well as the breeding, rearing, and training practices of polo horses. Frequently occurring ailments, such as musculoskeletal injuries, respiratory diseases, and internal illnesses, are highlighted. Lameness is a major problem, with injuries to the superficial digital flexor tendon being the most common cause. Other notable diseases include exercise-induced pulmonary hemorrhage (EIPH), myositis, rhabdomyolysis, and equine infectious anemia (EIA). To ensure the welfare of polo horses, effective prevention and management strategies are crucial. These include proper training, the adaptation of the ground surface, appropriate shoeing, and compliance with animal welfare guidelines and association rules. Collaboration between associations, players, organizers, and veterinarians is crucial. Promoting responsible management practices and raising awareness among stakeholders can help ensure that polo continues to thrive while maintaining high animal welfare standards.

The Importance of Animal Welfare and Country of Origin in Consumer Preferences: A Cross-National Study

Livestock husbandry systems are controversially discussed in society, politics and science. Since exporting countries such as Germany, the Netherlands or Denmark produce pork under higher animal welfare standards, the question arises if it will be competitive on the international market. We used a factor and cluster analysis to identify consumer segments in Japan, South Korea, Italy and Poland and to determine the instruments necessary to make animal welfare more popular. Each study country revealed three consumer groups: One group is interested in animal welfare and meat quality attributes but prefers domestically produced pork. Another group is price sensitive and without expectations. Finally, a third group is interested in animal welfare and accepts imported products. To address consumers in this group, informational campaigns and target market-oriented strategies are necessary.

The South Korean Perspective on German Animal Welfare Certified Pork

Industry stakeholder requests for improved animal welfare practices in German meat production have steadily increased in recent years. A transformation of German animal husbandry systems would almost certainly result in higher production costs. Against this background, producers and the meat industry are concerned about losing their international competitiveness while implementing improved animal welfare practices. As South Korea is an established trading partner for Germany, the objective of this explorative study is to assess South Korean meat market trends with a special focus on meat quality and animal welfare in order to evaluate export chances for German pork produced under higher animal welfare standards. Using a triangulation, the results of semi-structured interviews (n=15) with South Korean pork supply chain stakeholders are combined with the results of an online survey of South Korean consumers (n=723). Results indicate that the market potential for German meat produced under higher animal standards is still low in South Korea. Stakeholders associate improved husbandry conditions with healthier animals, benefits to human health and rank animal welfare as a key element of sustainability. However, due to consumers’ lack of knowledge and their price sensitivity, animal welfare is neither a unique selling point, nor linked to meat quality.

Quality Management and Animal Welfare as Culture of Care for Animal Experiments under Biosafety Level-3, taking COVID-19 as a Practical Example

Abstract Since the start of the COVID‐19 pandemic, a tremendous amount of work has been done by scientists to understand the pathogenesis of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Several animal models have been reported to be useful for the development and testing of vaccines, antivirals, or therapeutics. These in vivo experiments have to be performed at high containment biosafety level (BSL)-3. Thus, an appropriate biosafety, facility and data management as well as high standard of animal welfare, thorough study design, and staff training combined with an overall research transparency and open communication need to be implemented to achieve high quality reproducible research. Such an approach combined with the well-known 3R principle (Replacement, Reduction, and Refinement) and the 3S principle (Good Science, Good Sense, and Good Sensibilities) can contribute to culture of care in scientific research. The perspective reviews the key points of optimized biosafety, data, quality and animal welfare management in high risk biosafety-settings to conduct animal experiments with infectious pathogens in the context of a culture of care.

To trust or not to trust? Trust landscape of organic animal husbandry: Mapping consumer attitudes and information demands in Germany

A mainly positive attitude of consumers towards organic animal husbandry with its higher keeping standards compared to the legal regulations is evident. However, less is known about consumers’ detailed expectations of organic husbandry and in particular their attitude and trust along the value chain of organic animal products. Which consumers trust the most, and how do they want to be informed about organic animal husbandry? Where along the chain are trust deficits that should be addressed in the future to support sustainable food consumption with high animal welfare standards? To answer these important questions a survey was conducted among 729 German meat consumers. Using a cluster analysis, various consumer groups were identified based on their trust levels. Further, these groups were characterized regarding their general attitude, their information behavior, their evaluation of current media reporting, and their preferred way to be informed (emotional/rational) about organic animal products. The results revealed three clusters that clearly differ in their trust level of organic husbandry. Respondents assigned to the first cluster are committed organic consumers with high trust and the most positive attitude. The second cluster, combines respondents who are generally open to organic meat consumption and showing the second highest trust level and positive attitude towards organic. They show a slight favor for a more rational presentation of information. The third cluster is the smallest and can be described as the skeptics with a low interest in organic. The results indicate a general moderate to high trust level along the value chain of organic animal products, with the highest trust in organic retailers and farmers and the least trust in processing and conventional retailers. These are important insights for the organic sector in order to improve consumer trust and therewith increase the market share of organic meat products.

Dolphin-WET-Development of a Welfare Evaluation Tool for Bottlenose Dolphins (Tursiops truncatus) under Human Care.

Ensuring high standards of animal welfare is not only an ethical duty for zoos and aquariums, but it is also essential to achieve their conservation, education, and research goals. While for some species, animal welfare assessment frameworks are already in place, little has been done for marine animals under human care. Responding to this demand, the welfare committee of the European Association for Aquatic Mammals (EAAM) set up a group of experts on welfare science, cetacean biology, and zoo animal medicine across Europe. Their objective was to develop a comprehensive tool to evaluate the welfare of bottlenose dolphins (Tursiops truncatus), named Dolphin-WET. The tool encompasses 49 indicators that were either validated through peer review or management-based expertise. The first of its kind, the Dolphin-WET is a species-specific welfare assessment tool that provides a holistic approach to evaluating dolphin welfare. Inspired by Mellor's Five Domains Model and the Welfare Quality®, its hierarchical structure allows for detailed assessments from overall welfare down to specific indicators. Through combining 37 animal-based and 12 resource-based indicators that are evaluated based on a two- or three-level scoring, the protocol offers a detailed evaluation of individual dolphins. This approach allows for regular internal monitoring and targeted welfare management, enabling caretakers to address specific welfare concerns effectively.

Mouse and Rat Anesthesia and Analgesia.

Anesthesia and analgesia play pivotal roles in ethically and humanely using animal models in research, especially concerning mice and rats. These rodent species, extensively utilized in scientific investigations due to their genetic resemblance to humans, serve as invaluable tools for studying diseases and testing treatments. Proper anesthesia and analgesia not only prioritize animal welfare but also heighten experimental validity by minimizing stress-induced physiological responses. Recent years have seen remarkable advancements in anesthesia for mice and rats. The focus has shifted away from the 'one size fits all' toward tailoring anesthesia protocols, considering factors like age, strain, and the nature of the experimental procedure. The use of inhalation agents such as isoflurane and sevoflurane is often preferred due to their rapid induction and recovery characteristics, allowing precise control over anesthesia depth. However, refinements in injectable anesthetic agents also provide researchers the flexibility to select suitable agents based on study requirements. Additionally, progress in analgesic techniques has led to effective pain management strategies for these rodents. Common analgesics such as nonsteroidal anti-inflammatory drugs (NSAIDs), opioids, and local anesthetics are administered to alleviate pain and discomfort. However, standard practice also involves continuous monitoring of animals' behavior and physiological parameters, ensuring timely adjustments in analgesic regimens for optimal pain relief without compromising experimental outcomes. By integrating tailored anesthesia and analgesia protocols into the experimental design, researchers uphold high animal welfare standards while obtaining reliable scientific data. This contributes significantly to advancing medical knowledge and therapeutic interventions with reproducible results. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Injectable anesthesia for mouse and rat Basic Protocol 2: Inhalant anesthesia using isoflurane for mouse and rat Basic Protocol 3: Analgesia for mice and rats.

VETERINARY, ECONOMIC AND SOCIAL ASPECTS OF CATTLE WELFARE: A REVIEW

The paper presents a review of the literature on cattle welfare. The objective of this review is to outline the veterinary, economic, and social aspects of cattle welfare and highlight the current problems, global trends, and solutions. The global major scientific reference databases (Web of Science, ScienceDirect, Scopus) were reviewed. The review brought the following findings: a change in scientific and practical approaches to the understanding and assessment of the welfare of productive animals that requires not only health maintenance, stress prevention, or the ability of animals to express natural behavior. Animal welfare gains more anthropometric traits, including such concepts as 'pleasure' or 'happiness'. The concept of 'positive welfare' has become a more popular trend in scientific research on animal welfare. The review identified positive trends of possible modification of production systems, to make them more diverse and animal welfare friendly. The research found livestock products consumers growing concerned about animal welfare and housing conditions. The concept of welfare is more commercialized, which encourages livestock producers to adhere to ethical production standards. However, the demand of consumers to meet high standards of animal welfare does not have sufficient economic leverage. Only a few are willing to pay more for the better welfare of the animals or to refuse to consume animal products from production systems with poor welfare conditions. This review was intended to draw the attention of scientists, farmers, and consumers to the problems of the humane treatment of productive animals. And to assist stakeholders in finding their place in the process of creating better conditions for animals that are used for food, helping people with their existence or with their lives.

Willingness to Pay a Higher Price for Pork Obtained Using Animal-Friendly Raising Techniques: A Consumers' Opinion Survey.

In Italy, the consumption of pork meat is increasing, despite consumers' attitudes being addressed toward a greater sensitivity about animal welfare and its link with safe food. Considering the relatively high animal welfare standards and the divergence in public interest in farm animal welfare and ethical issues, the objective of this study-in continuation of our previous paper relating to consumer behavior and preferences in welfare-friendly pork breeding-was to investigate habits of pork consumers regarding pig welfare, principally evaluating their willingness to pay (WTP) a higher price for pork obtained using raising techniques with an approach based on animal welfare. An ad hoc questionnaire-based survey was submitted to consumers (n = 404) in Messina province, Italy. Results suggest that 47% of consumers were willing to pay an additional price for pork from farms that apply specific animal welfare standards. Positive correlations were between WTP and farming techniques (p = 0.001), organic farming methods (p = 0.001), and farms in which animal welfare is taken care of and guaranteed (p < 0.001). These findings suggest that consumers intend to pay a higher price for pork, like other animal products obtained using animal-friendly raising techniques. The sensitivity to the animal welfare of a single human being may influence consumers' attitudes toward pork consumption.

Environmental Impacts of High-Quality Brazilian Beef Production: A Comparative Life Cycle Assessment of Premium and Super-Premium Beef.

When individual purchasing power increases in society, there is a trend toward a quantitative and qualitative increase in the consumption of products. Considering the magnitude of beef production in Brazil, environmental impacts are important factors for the domestic and international markets. This study assessed a Brazilian feedlot system characterized by high animal welfare standards that produces high-quality beef that is more marbled than that produced in grass-fed systems. We assessed the environmental impacts and compared premium and super-premium beef produced in a feedlot system using a partial Life Cycle Assessment (LCA). Data were collected directly on the property analyzed, considering environmental inputs and outputs and economic factors associated with the production stages of each line (premium versus super-premium). The results show that high-quality beef has, beyond a greater financial cost, an environmental cost, with the super-premium line producing a 286% higher carbon footprint, 297% more eutrophication, and three times higher acidification potential and land use than the premium line. The results of the environmental impacts agree with the results of production costs, reflecting a 282.82% higher production cost in super-premium than in premium animals. Footprints of 5.0323 kg, 4.7746 kg, and 8.8858 kg CO2 eq./kg live weight gain at the feedlot were found in the three lines.

47 Navigating the Biotechnology Landscape in Animal Agriculture: Consumer and Producer Acceptance of Gene Editing to Improve Farm Animal Welfare

Abstract Consumer demand for production process traits in food and agricultural products is rapidly increasing. Recent legal measures reflect the demand for high animal welfare standards. At the same time, consumers are wary of biotechnology applications in food and agriculture, with calls for natural or organic production across the industry. However, when biotechnologies are applied for the benefit of the consumer or address a consumer concern, they become more acceptable. Producers, however, may be hesitant to adopt biotechnologies, given concerns about market acceptance. This presentation will explore the economic foundations, challenges, and opportunities for consumer and producer acceptance of biotechnology applications in animal welfare, especially gene editing techniques. Using the pork and diary industries as case studies, this talk will discuss the emerging opportunities for future improvements through gene editing. Understanding the economic foundations of biotechnology used to improve animal welfare aids agricultural producers, scientists, and policymakers to make optimal decisions regarding how these important aspects of animal agriculture progress.

How the EU animal welfare legislation can tackle AMR – a One Health perspective

Abstract The development of antimicrobial resistance (AMR) is linked to the imprudent use of antimicrobials and is fuelled by industrial farming, accounting for 70% of antimicrobial use. Tackling AMR requires a One Health approach that addresses the root causes of high antimicrobials use in intensive farming and generates co-benefits for human, animal and environmental health. On intensive farms animals are mostly of high-yield breeds and are exposed to stressors, which leads to immunosuppression, increasing susceptibility to infections that need antimicrobial therapy. To combat AMR the EU Commission adopted legislation prohibiting the use of antimicrobials to compensate for poor husbandry and management. However, there is a gap in policy regarding animal welfare improvements necessary to reduce the need for antimicrobials. We assess how the EU animal welfare legislation, under revision, can contribute to filling this gap by providing the regulatory framework for improvements needed to reduce antimicrobial use. Methods include literature review and expert interviews in an approach that integrates knowledge across the human-animal-environmental sectors. Improvements in husbandry and management (lower stocking density, outdoor access, genetic diversity, etc.) positively impact animal health and welfare, consequently reducing susceptibility to infections and antimicrobial use. By prescribing these improvements the revised animal welfare legislation can have a synergistic effect with the Veterinary Products Regulation, in line with the Farm to Fork strategy, and provide the regulatory framework for higher animal welfare standards that reduce antimicrobial use. The positive correlation between higher animal welfare and the reduced need for antimicrobials demonstrates the urgency of improving welfare in farming as a key strategy to limit AMR. It builds the case for an ambitious revision of the EU animal welfare legislation to protect animal and human health in line with One Health. Key messages • The revised EU animal welfare legislation can become a relevant policy in tackling AMR by providing the regulatory framework for high animal welfare standards neded to reduce antimicrobials in farming. • Tackling AMR requires a One Health approach addressing the root causes of high antimicrobial use in intensive farming: the poor welfare of animals, which increases their susceptibility to infections.

Milking Machine Settings and Liner Design Are Important to Improve Milking Efficiency and Lactating Animal Welfare—Technical Note

The purpose of milking machines is to harvest milk at optimal quality and speed, while maintaining animal comfort and teat defense mechanisms against invading mastitis pathogens. Therefore, the milking machine is a very important piece of equipment on dairy farms to maintain a long healthy lactation by following the physiological conditions of the udder. The mechanical forces during long-term machine milking processes lead to changes in the teat tissue. This effect is related to the degree of adaptation of the milking machines to the physiological requirements of the individual udder anatomy and the physiological conditions of the lactating animals. If both, milking machine settings and liner design are not suitable for all teats and animals on the farm, some animals will not be fully milked, the teat condition will deteriorate over time and in the end, they may suffer from mastitis. Therefore, maintaining healthy udders and teats during milking is a central key component of an effective milking machine to produce good milk yield with higher quality by preventing mastitis and maintaining animal health and welfare. On large and thick teats, conventional liners often fit too tight, causing a massive mechanical stress load on the tissue. On small teats, however, they often do not adhere sufficiently close to the teat which can cause a considerable air admission and hence liner slips. The new liners, “Stimulor® StressLess” (Siliconform, Türkheim, Germany), have a wave-like lip construction and adapt well to the different teat sizes in a herd, thus ensuring consistent milking of lactating animals. A proper milking machine accommodates all teat sizes and forms, has a low vacuum to effectively open the teat and to stimulate physiological milk release and letdown. In addition, the right pulsation rate will maintain a stable vacuum on the teat area during milking. In conclusion, an ideal milking machine adapts to the morphological, anatomical, and physiological characteristics of the udder and teats of the lactating animals and it should achieve a physiologically ideal milking process that meets high animal welfare standards and increases milk production with a high quality standard.

A Practical Approach to Providing Environmental Enrichment to Pigs and Broiler Chickens Housed in Intensive Systems.

In Europe, regulations contain guidance to maintain high standards of animal welfare. In many parts of the world, large buyers for supermarkets or restaurants are the main enforcers of basic animal welfare standards. They can have considerable influence on improving standards on large commercial farms. Research clearly shows that straw is one of the most effective environmental enrichment for pigs. On some large farms, there are concerns that straw will either clog waste management systems or bring in disease. This paper contains a review of both scientific research and practical experience with enrichment devices that are easy to implement. Pigs prefer enrichment objects that they can chew up and deform. Broiler chickens prefer to climb up on objects, hide under them or peck them. It is always essential to uphold basic welfare standards such as animal cleanliness and low levels of lameness (difficulty walking). It is also important to reduce lesions, and maintain body conditions of breeding animals. An environment enrichment device is never a substitute for poorly managed facilities. It should enhance animal welfare on well-managed farms.

The impact of information about animal husbandry systems on consumers’ choice of meat products in a virtual supermarket

Farm animal welfare has become a frequently discussed issue. However, the consumption of animal-friendly products remains a small part of total meat consumption. This study investigated whether providing consumers with more detailed information about animal husbandry systems could influence their product choices at a virtual supermarket. Participants (N = 124) were randomly assigned to one of three experiment conditions, which differed in terms of the information provided: 1) only animal-welfare label (control condition), 2) animal-welfare label and a positive video describing an organic animal husbandry system (AHS) (positive video condition), and 3) animal-welfare label, a positive video, and a negative video describing a conventional AHS (positive & negative video condition). In all conditions, participants needed to select an ingredient for a curry dish from three presented products, i.e., a conventional chicken breast, an organic chicken breast, and a plant-based meat alternative that looks like chicken meat. The results indicated that participants’ product choices were not significantly different between the three conditions. As shown by eye-tracking data, participants paid little attention to the animal-welfare-related information. Price was the most important information for participants’ purchasing choices. Also, participants’ meat-eating justification (MEJ) strategies were negatively correlated with their choice of products produced with higher animal-welfare standards. These results illustrate that providing more information about animal-welfare or the conditions under which animals are farmed alone is not enough to induce significant changes of consumers’ meat shopping behavior. The price of the products and participants’ MEJ strategies play important roles in consumers’ purchase decisions.

Welfare impacts associated with using aversive geofencing devices on captive Asian elephants

Animal-borne aversive geofencing devices (AGDs, or satellite-linked shock collars) are commercially available and used on livestock to restrict their movement within a virtual boundary. This technology has potential application as a human-wildlife conflict mitigation tool, where problem animals might be conditioned to avoid human-dominated habitats by associating an audio warning with a subsequent electric shock, which is delivered if the audio warning is ignored. Ensuring that high standards of animal welfare are maintained when implementing such tools is important for acquiring manager and community acceptance of such approaches. We conducted two pilot experiments with eight captive Asian elephants using mild electric shocks from a modified dog-training collar fitted around the neck, as part of an ongoing effort to develop AGDs suitable for mitigating human-elephant conflict. As part of these experiments, we assessed elephants' behavioural and physiological stress before, during and after the experiments. During the experiments elephants wore collars for up to nine consecutive days and received a small number of electric shocks on 1–3 consecutive days. Bootstrapped principal component analysis showed that daily activity budgets of individual elephants on experiment days were not different from the pre-experiment days. Generalised linear mixed-effects model (GLMM) showed that anxiety/stress behaviours increased on the first day of acclimatising to the collar and on testing days (i.e. days they received shocks) of the first experiment, but not during the second experiment relative to pre-experiment days. Analysis of faecal cortisol metabolite (FCM) concentrations using GLMM showed that FCM concentrations were higher in samples collected ∼24 hrs and ∼48 hrs after testing days compared to baseline levels as expected given the lag time for excretion of cortisol metabolites. These elevated anxiety/stress behaviours and FCM concentrations returned to baseline levels shortly after the experiment. Therefore, we conclude that AGDs did not produce lasting behavioural or physiological stress effects in elephants during this short-term study but recommend further studies with a larger sample of elephants to confirm the transferability of these findings.