High Pump Research Articles

Use of Sustainable Energy Sources and Technologies in Primary and Secondary School Buildings in Greece. Can They Eliminate their Carbon Footprint?

School buildings consume energy covering their energy demand. The most of them utilize fossil fuels and grid electricity while the use of renewable energies is rather limited. School buildings should eliminate their carbon emissions in order to achieve the global target for net-zero carbon emissions by 2050. The energy consumption and the carbon emissions in schools have been evaluated. School buildings in Greece consume less than 100 kWh/m2 year. Solar thermal energy, solar photovoltaic energy and high efficiency heat pumps can cover all the energy demand in school buildings in Greece replacing the use of conventional energy sources. These energy technologies are reliable, mature and cost-effective. There are 7,756 primary and secondary school buildings in Greece consuming 1.67 TWh/year. The total cost of energy renovation eliminating their carbon emissions is estimated at 1,156,000 €/school or 8.96 billion € for the 7,756 schools in the country. For achieving the target of net-zero carbon emissions by 2050, 310 Greek schools should be energy refurbished every year eliminating their carbon footprint in the next 25 years. Taking into account the availability of benign energy sources and technologies the main barrier for eliminating the carbon emissions in school buildings in Greece by 2050 is the high investment cost. Our results could be useful for the development of the required policies for decarbonization in school buildings.

Groundwater Model for Karst and Pelitic Aquifer Systems from a Semi-Arid Region Under Climate Change Scenarios: A Case Study in the Vieira River Watershed, Brazil

Water scarcity is a global issue, especially in semi-arid and arid regions where precipitation is irregularly distributed over time and space. Predicting groundwater flow in heterogeneous karst terrains, which are essential water sources, presents a significant challenge. This article integrates geology, hydrology, and water monitoring to develop a pioneering conceptual and numerical model of groundwater flow in the Montes Claros Region (Vieira River Watershed, Brazil). This model was evaluated under various climate change scenarios, considering changes in rainfall, groundwater consumption, and population growth over the current century. The results indicate that a decline in water table levels is inevitable, primarily driven by population growth and high pumping rates rather than rainfall fluctuations. This underscores the urgent need for improved monitoring, model upgrading, and more importantly, targeted water resource management for Montes Claros.

Construction and evaluation of transdermal delivery system of terbinafine-loaded plant exosomes for the treatment of cutaneous fungal infections

Local transdermal of antifungal agents is a problem that limits the effectiveness of clinical treatment of dermatomycosis. In this study, cucumber-derived plant extracellular vesicles (PEV) were extracted and loaded with the antifungal drug terbinafine (TBF) to form TBF-PEV, which was applied locally to the site of skin fungal infection to improve the transdermal transport capacity of TBF and the antifungal effect in vitro and in vivo. Chemical enhancer and physical enhancer methods were replaced by low immunogenicity and non-irritating PEV. TBF-PEV caused obvious curling and wrinkling in the skin, induced changes in the distribution of skin lipids, and changed the structure and content of skin lipids and proteins, thereby increasing the transdermal efficiency of TBF. The local drug concentration was increased, and the antifungal effect was improved in vitro and in vivo. It was first found that PEV inhibited the growth of Candida albicans with high efflux pump expression. In general, this paper has certain significance in local transdermal treatment of fungal skin infections and reversal of fungal resistance.

High temperature heat pumps for industrial heating processes using water as refrigerant

High temperature heat pumps for industrial heating processes using water as refrigerant

Numerical investigation into the acid flow and reaction behavior in the tight, naturally fractured carbonate reservoir during acid fracturing

Tight naturally fractured carbonate reservoirs require acid fracturing to build the connectivity between the wellbore and natural fractures (NFs), where hydrocarbon is stored. The high leakoff nature of the NF complicates the acid flow and etching pattern, raising the difficulty in acid fracturing design and optimization. To explore the acid flow and reaction behavior in such reservoirs, an acid fracturing model accounting for the NF distribution is developed, which consists of a fracture surface characterization model, a fracture propagation model, and an acid-etching model. Based on the model, the effects of injection parameters and the NF properties on the effectiveness of acid fracturing are investigated. Then, strategies for acid fracturing the tight naturally fractured carbonate reservoirs are proposed. Results show that high pumping rates and retarded acids with low hydrogen ion (H+) diffusion coefficient is conducive to achieving a long acid penetration distance, while a low pumping rate and acids with a high H+ diffusion coefficient facilitates the NF etching. Therefore, a large stimulated area can be achieved by applying a multi-stage alternating injection of the crosslinked acid with a high pumping rate followed by the gelled acid with a low injection rate. NFs impact acid fracturing in two distinct ways: enhancing the non-uniform etching of the fracture surface and reducing the effective acid-etched fracture length through high leakoff. When NF density is high, leakoff control techniques should be employed; and when the NF inclination is high, non-uniform etching techniques should be used to generate acid-etched channels in flow barriers.

The true incidence and risk factors of hemolysis in patients with Impella CP placement

Abstract Background The Impella, a micro axial-flow pump, is effective in treating cardiogenic shock. However, compared to other mechanical circulatory support devices, its high pump speeds can result in complications like hemolysis and subsequent renal failure. Previous reports have defined hemolysis based on the elevation of LDH levels or the presence of hemoglobinuria, but this approach may underestimate its occurrence. Hemolysis is commonly assessed using the hemolysis index, which is calculated through biochemical auto-analysis based on the absorbance measure of the red color change. However, since the hemolysis index is not highly sensitive, it is recommended to measure plasma free hemoglobin (PFHb) for accurate diagnosis of hemolysis. Purpose The aim of this single-center retrospective cohort study is to investigate the frequency of hemolysis in Impella CP using PFHb measurements and its association with the Impella support level. Methods Between December 2019 and June 2023, we included 116 patients who had the Impella CP device implanted at our hospital. For these patients, PFHb was measured twice daily during the Impella placement period (up to 13 days). A PFHb value of 0.06 or higher was considered indicative of hemolysis, at which point a haptoglobin preparation of 4000 units was administered. Separately, the hemolysis index was defined as hemolysis at a level of 41 mg/dL. We investigated the relationship between daily Impella settings, PFHb, and hemolysis index for each patient. Results The average age was 70 ± 13 years. 59% underwent ECPELLA, and 15% were introduced to renal replacement therapy (RTT) due to severe renal failure. During Impella placement, hemolysis was diagnosed in 33% of patients based on PFHb, while only 18% were diagnosed with hemolysis according to the hemolysis index. Upon separate analysis for Impella and ECPELLA during the initial three days, the support levels of Impella were significantly higher in the Impella group on Days 1 and 2, with no significant difference noted on Day 3. Moreover, PFHb levels were significantly higher in the Impella group on Day 1, while on Day 3, the levels were higher in the ECPELLA group. Patients on RRT had significantly higher max PFHb levels than those not on RRT (0.19 vs. 0.06 g/dL; p < 0.001). Next, we investigated the relationship between PFHb levels and Impella support levels in patients with either Impella or ECPELLA over 711 data points. Dividing Impella support into low (P1-3), middle (P4-6), and high (P7-9) categories, PFHb values were significantly higher at high P levels compared to low and middle P levels. Similar results were observed for both Impella and ECPELLA. Conclusion There is a possibility of underestimating hemolysis associated with Impella if PFHb measurement is not performed. Hemolysis showed a significant correlation with higher P levels (P7 and above) for both Impella and ECPELLA.

Techno-Economic Comparative Assessment of High Temperature Heat Pump Architectures for Industrial Pumped Thermal Energy Storage

Abstract The industrial sector is a key global source of wealth, but it is also recognized as a major challenge toward worldwide decarbonization. Today the industrial sector requires more than 22 % of the global energy demand as thermal energy and produces for this about 40 % of the total CO2 emissions. Solutions to efficiently decarbonize the industrial sector are deemed. This work presents a comparative techno-economic performance assessment of a high temperature heat pump integration within a molten salts based power to heat system for industrial heat flexible generation. The main system performance is reported in terms of required working conditions and temperature for the heat pump and thermal demand size as well as reduction of the attainable levelized cost of heat (LCoH) against non-flexible electric boiler based systems. The impact of different industrial load profiles, electricity prices, heat pump capital cost and heat pump real to Carnot efficiency ratio are also presented. The results highlight that the proposed system can be cost-competitive, particularly for thermal demand around 10 MW and waste heat temperatures above 80°C. Under these conditions LCoH reductions higher than 15 %, with respect to the considered non-flexible electric boiler alternative, are attainable. These LCoH reductions are primarily driven by savings in electrical consumption as high as 30 %. This study sets the ground for further power-to-heat techno-economic investigations addressing different industrial sectors and identifies main system and components design strategies, integrations, and targets.

Aquiferous system, filtration rates and hydrodynamics of the syconoid calcareous sponge Urna sp.

The aim of the present study was to gain insight into the hydrodynamic characteristics of the relatively simple aquiferous system in specimens of a calcareous syconoid sponge, Urna sp. Data on the morphology and ultrastructure of the sponge combined with measured pumping rates were provided and used for subsequent estimates of the pressure drops of water flow through the aquiferous system. The pumping rates were estimated from microscope video-recordings as the product of osculum-cross sectional area and exhalant jet speed. Estimates are given of the sensitivity of pressure drops to dimensional changes associated with observed dynamic, contractile structures (e.g., osculum, apopyles), as well as possible artefacts introduced in the preparations for ultrastructural studies. The estimated pressure losses showed that the choanocyte pumps provide a pressure of 5.5 ± 3.9 Pa at a pumping rate of 1533 ± 1089 µm3 s−1 per choanocyte. Such high pumping rates, comparable to those of some choanoflagellates, have not been reported for syconoid and leuconoid sponges before. However, the corresponding sponge volume-specific pumping rates (about 10–30 min−1) are comparable to values reported in the literature for small sponges and explants that also have relatively higher pumping rates of choanocytes than larger sponges.

Vortex Motion on the Surface of Shallow and Deep Water

The attenuation of vortex motion on the surface of shallow and deep water is investigated. The vortex flow was formed by two mutually perpendicular waves excited by plungers at a frequency of 6 Hz (wavelength λ = 5.6 cm) on the surface of water measuring 70 × 70 cm and depth h. After reaching a stationary state in the vortex system, the wave pumping was switched off, and the attenuation of the surface flow was recorded. On the surface of shallow water, λ/2π ≈ h, when the characteristic size of the vortices L exceeds the depth of the liquid, L h, the time dependence of the energy of the vortex flow Е(t) is described by an exponential function at all pumping levels, and the dependence of the enstrophy Ф(t) = Ω2(t) has an exponential dependence only in the range of wave vectors 0–0.3 см–1. On the surface of deep water λ/2π h, L ≈ h dependence Е(t) and Ф(t) are far from exponential in all ranges of wave numbers. At high pumping levels, the dependencies Е(t) and Ф(t) are nonmonotonic, which can be attributed to the influence of volumetric flows.

Numerical investigation of high temperature heat pump integrated hybrid cooling system

Over 67 % of the energy demand within the pharmaceutical and semiconductor industries is for their cleanrooms, driven by stringent temperature and humidity control requirements. Despite high energy usage, heating, ventilation, and air-conditioning (HVAC) units consisting of conventional vapor compression systems (CVCS) are favoured for their simplicity. Hybrid cooling systems (HCS) offer potential efficiency gains by separating latent and sensible loads. Still, reliance on fossil-fuel-fired or electric heaters to regenerate the desiccant makes it energy intensive and reduces CO2 mitigation benefits. This study explores high-temperature heat pumps to simultaneously cool process air and heat regeneration air, thereby using multiple benefits. Heat pumps with condensing temperatures of 80 °C, 90 °C, and 120 °C and an ejector-enhanced trans-critical CO2 heat pump were compared with CVCS and HCS. The 120-bu and CO2HP configurations demonstrated a system COP of 2.94 and 3.22, respectively, surpassing CVCS, which has a system COP of 1.44. These setups achieved CO2 emission reduction of 51 % and 55.2 %, compared to CVCS, respectively.

Blood trauma in veno-venous extracorporeal membrane oxygenation: low pump pressures and low circuit resistance matter

BackgroundVeno-venous extracorporeal membrane oxygenation (VV ECMO) has become standard of care in patients with the most severe forms of acute respiratory distress syndrome. However, hemolysis and bleeding are one of the most frequent side effects, affecting mortality. Despite the widespread use of VV ECMO, current protocols lack detailed, in-vivo data-based recommendations for safe ECMO pump operating conditions. This study aims to comprehensively analyze the impact of VV ECMO pump operating conditions on hemolysis by combining in-silico modeling and clinical data analysis.MethodsWe combined data from 580 patients treated with VV ECMO in conjunction with numerical predictions of hemolysis using computational fluid dynamics and reduced order modeling of the Rotaflow (Getinge) and DP3 (Xenios) pumps. Blood trauma parameters across 94,779 pump operating points were associated with numerical predictions of shear induced hemolysis.ResultsMinimal hemolysis was observed at low pump pressures and low circuit resistance across all flow rates, whereas high pump pressures and circuit resistance consistently precipitated substantial hemolysis, irrespective of flow rate. However, the lower the flow rate, the more pronounced the influence of circuit resistance on hemolysis became. Numerical models validated against clinical data demonstrated a strong association (Spearman’s r = 0.8) between simulated and observed hemolysis, irrespective of the pump type.ConclusionsIntegrating in-silico predictions with clinical data provided a novel approach in understanding and potentially reducing blood trauma in VV ECMO. This study further demonstrated that a key factor in lowering side effects of ECMO support is the maintenance of low circuit resistance, including oxygenators with the lowest possible resistance, the shortest feasible circuit tubing, and cannulae with an optimal diameter.

Different ECLS Pump Configurations for Temporary Right Ventricular Assist Device in LVAD Patients: A Retrospective Case–Control Study

Background: Acute right ventricular failure is a critical complication after left ventricular assist device (LVAD) implantation, often managed with a temporary paracorporeal right ventricular assist device (RVAD). This study examined three extracorporeal life support (ECLS) systems regarding mortality, bleeding complications, and intensive care unit (ICU) stay duration. Methods: This monocentric, retrospective case–control study included all patients receiving LVAD with paracorporeal RVAD between 2009 and 2020. Three patient groups were formed: CentrimagTM (A), CardiohelpTM (B), and DeltastreamTM (C). Results: A total of 245 patients were included. Preoperative parameters were similar between the CentrimagTM and DeltastreamTM groups, but CardiohelpTM patients had worse Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) Scores (A: 1.7 ± 0.8, B: 1.36 ± 0.5, C: 1.9 ± 0.9; p < 0.05). In-hospital death rates were A: 61 (41.8%), B: 15 (32.6%), C: 29 (54.7%); p < 0.05, and reoperation due to bleeding rates were A: 32 (21.9%), B: 8 (17.4%), C: 25 (47.2%); p < 0.05, with the DeltastreamTM group showing the highest rates. This group also had increased thrombocyte consumption and prolonged ICU stays. Conclusions: Temporary RVADs lead to bleeding complications, affecting patient outcomes. The DeltastreamTM group had significantly higher bleeding complications, likely due to high pump revolution rates and thrombocyte decline. Due to the study’s retrospective nature and complex patient profiles, these interesting findings should be validated in future studies.

Study on the Internal Flow Characteristics of High Flowrate Emergency Drainage Series-parallel Pump

Abstract Floods have become the main natural disaster threatening the safety of peoples lives and property. It is of great significance to develop high flow emergency drainage equipment. An innovative structure of pumps with high flow rate and multiple operating conditions is proposed in this paper to achieve series and parallel operation. It established an automatic optimization platform that integrates functions such as 3D modeling, mesh generation, computational fluid dynamics, and scheme optimization, and developed an efficient hydraulic model for a volute mixed flow pump combining flow field diagnostic techniques. The research results indicate that the inlet distortion of the secondary stage pump is the main cause of pump performance loss for both series and parallel operation condition. The third-generation vortex identification method was used to diagnose the internal flow of the pump under two operating conditions and control strategies were proposed. The final optimization scheme show that the pump can reach 1800 m3/h with a head of 9.6m and efficiency of 80% in parallel operation condition. In series operation condition, the pump can reach 900 m3/h with a head of 18.3m and an efficiency of 75.3%.

The mechanism of proppant transport dynamic propagation in rough fracture for supercritical CO2 fracturing

Supercritical CO2 fracturing technology has shown great potential for enhancing production in unconventional reservoirs. It is essential to clarify the transport mechanism of proppant under the dynamic propagation conditions within rough fractures. A realistic rough fracture model is reconstructed, and Computational Fluid Dynamics simulations are conducted to track proppant movement during fracture propagation. The typical transport characteristics of proppant within rough fractures are revealed, and the effects of fracture propagation rate, proppant density, mass flow rate, particle size, sand ratio, and temperature on the support effect are discussed. The results show that the flow channels formed by sand carrying fluid in rough fractures are complex, with fracture propagation changing some flow channels. The proppant forms an irregular sand bed interspersed with unfilled areas, and complex flow characteristics are generated. The increase in fractal dimension increases the resistance in the fluid flow process and affects the movement of the proppant, which tends to create unfilled areas. Low density and size of proppant can improve the proppant placement length. In a certain temperature range, high temperature injection of sand carrying fluid can improve the proppant placement effect. In addition, the low sand ratio and high mass flow rate pumping can be used to form the dominant channel, followed by pumping with a high sand ratio and low mass flow rate for effective support.

Selection of refrigerant based on multi-objective decision analysis for different waste heat recovery schemes

Waste heat generation, upgrading, and refrigeration are the fundamental ways to recover and utilize waste heat. The rationalizing the use of refrigerants also contribute in creating energy savings and minimizing carbon emissions. This study evaluated the thermodynamics, economics, and environmental of different types of refrigerants in three kinds of waste heat recovery schemes: generate electricity, heat pump, and refrigeration. Based on this, the entropy weight and Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) were combined to assess the overall performance of the refrigerants. A thorough analysis revealed that R1234ze(E) can replace R245fa and R123 in the organic rankine cycle (ORC). The best refrigerant for vapor compression refrigeration (VCR) and high temperature heat pump (HTHP) systems is R1243zf. In addition, the multi-objective decision analysis shows that the performance difference among the nine selected refrigerants is the total cost, followed by the environmental impact. The approach is successful in recognizing the variations between different refrigerants in the same waste heat recovery scheme and gives a thorough evaluation. It sets instructions for the future use of eco-friendly refrigerants and their application waste heat recovery schemes.

Fracture-controlled fracturing mechanism and penetration discrimination criteria for thin sand-mud interbedded reservoirs in Sulige gas field, Ordos Basin, China

Considering the problems in the discrimination of fracture penetration and the evaluation of fracturing performance in the stimulation of thin sand-mud interbedded reservoirs in the eighth member of Shihezi Formation of Permian (He-8 Member) in the Sulige gas field, a geomechanical model of thin sand-mud interbedded reservoirs considering interlayer heterogeneity was established. The experiment of hydraulic fracture penetration was performed to reveal the mechanism of initiation–extension–interaction–penetration of hydraulic fractures in the thin sand-mud interbedded reservoirs. The unconventional fracture model was used to clarify the vertical initiation and extension characteristics of fractures in thin interbedded reservoirs through numerical simulation. The fracture penetration discrimination criterion and the fracturing performance evaluation method were developed. The results show that the interlayer stress difference is the main geological factor that directly affects the fracture morphology during hydraulic fracturing. When the interlayer stress difference coefficient is less than 0.4 in the Sulige gas field, the fractures can penetrate the barrier and extend in the target sandstone layer. When the interlayer stress difference coefficient is not less than 0.4 and less than 0.45, the factures can penetrate the barrier but cannot extend in the target sandstone layers. When the interlayer stress difference coefficient is greater than 0.45, the fractures only extend in the perforated reservoir, but not penetrate the layers. Increasing the viscosity and pump rates of the fracturing fluid can compensate for the energy loss and break through the barrier limit. The injection of high viscosity (50–100 mPa·s) fracturing fluid at high pump rates (12–18 m3/min) is conducive to fracture penetration in the thin sand-mud interbedded reservoirs in the Sulige gas field.

Pure-quartic soliton self-frequency shift in a mode-locked fiber laser

The pure-quartic soliton (PQS) offers the advantage of a wide spectrum and approximately Gaussian temporal profile, which has the potential to obtain high-energy ultrafast laser pulses. Thus, exploring the role of stimulated Raman scattering in the formation and propagation of PQSs in fiber lasers is crucial, especially considering the greater propensity for highpower and short PQS pulses compared to traditional solitons. Here, we present the modeling of a self-frequency shift (SFS) fiber laser based on the PQS, emphasizing the impact of fourth-order dispersion and the Raman effect. The significant red-shift in wavelength is discovered as the pump energy increases, revealing that the emergence of SFS is a universal attractor in mode-locked PQS fiber laser systems. Our simulations demonstrate that such wavelength tunability is a direct byproduct of the gain bandwidth limit and the stimulated Raman scattering for PQSs inside the fiber cavity, while the evolution dynamics in the gain and passive fiber show obvious differences. However, we find that the effect of SFS in a PQS fiber laser is compromised by a trade-off with the degradation of pulse quality, such as reshaping the oscillating tail and destroying the symmetry of the emission pulse. This Raman-induced nonlinear process under high pump energy facilitates the discovery of the comprehensive complexity of science for PQSs suffering from higher-dimensional forms of nonlinear effects in fiber lasers.

Economic and environmental considerations for the deployment of industrial very high temperature heat pumps in European markets

Very high temperature heat pumps (VHTHP) have begun to emerge on the market during the past decade, providing an alternative to fuel-fire boilers for generating industrial process heat up to 200 °C. Large temperature lifts are common for VHTHPs, resulting in a reduced coefficient of performance (COP) compared to traditional heat pumps. Thus, the economic feasibility of operating VHTHPs becomes more dependent on local price conditions, and the environmental impact is affected by increased electricity usage. Both the economics and the environmental impact of operating industrial VHTHPs in place of fossil fuel-fired boilers in Europe are studied in this article. Discounted payback periods are estimated for the varying price conditions in Europe, and life cycle assessment tools are used to quantify the environmental impact of operating VHTHPs. Finland, Sweden, and Denmark were found to be ideal countries for VHTHP deployment, as they showcase both favorable economic conditions as well as generally small environmental impacts in the endpoint categories Human health, Climate change, and Resource usage, if local grid electricity is used. The effect on the endpoint category Ecosystem impacts was generally larger for most countries, including the aforementioned three, mostly due to the use of biomass in the electricity grid.

Fatigue and Autofrettage Analysis of Steel Sleeve Based on Precision Fit under Ultra High Pressure

Abstract In order to ensure precision fit between steel sleeve and plunger, copper sleeve and the other parts of the ultra high pressure plunger pump, as well as increase the fatigue life of steel sleeve, this paper made the analysis of work process and fatigue life of ultra high pressure autofrettage steel sleeve of plunger pump based on both analytical method and FEM. The elastic-plastic boundary radius, radial stress and plastic zone displacement were obtained based on the elastic-plastic mechanics analysis of ultra high pressure steel sleeve. The functional relationship built related to precision fit among autofrettage pressure, inner diameter expectation and displacement could ensure the machining dimension of inner diameter of steel sleeve and realize the precise control of inner diameter of steel sleeve. The analytical method and FEM for calculating fatigue life was also proposed, which are verified by experiment. The research shows that machining dimension is determined by inner diameter expectation in the autofrettage process to ensure precision fit; autofrettage treatment can reduce the working stress level of the steel sleeve and extend the fatigue life remarkably. These conclusions contribute to guide the design of steel sleeve.

Modulation optimization when using a splitter pump after the first dimension in comprehensive two- dimensional liquid chromatography

The rapid growth in the use of two dimensional liquid chromatography (2D-LC) applied to the analysis of moderately to highly complex mixtures, has been fueled by continuous improvements in performance and robustness of the instrument components, as well as the ease-of-use of software necessary for controlling the 2D-LC instrument hardware, and analysis of the large data files that result from this type of work.This work has focused on the evaluation of the performance of an online full comprehensive mode (LC×LC), when an active modulation is implemented using a flow splitter pump placed after the 1D effluent. Two different types of splitting pumps were evaluated: a binary ultra-high pressure liquid chromatography (UHPLC) pump and a high precision syringe pump. We analyzed the performance (reproducibility in peak area and retention times and the 2D peak dispersion) as a function of the location of the active pump Before or After the modulation valve, and the influence of connecting tubes (based on internal diameter and length) necessary between the interface, waste, and the splitting pump. The effect on the flow direction on the filling and flushing of the injection loops at the modulation valve was also analyzed for each pump.In this study, we demonstrate that flow-splitting LCxLC assembly can be performed using either a UHPLC binary pump or a simple syringe pump. Flow splitting after the first dimension is a straightforward strategy to: (i) independently select the 1D column and flow rates with respect to the second dimension; (ii) consciously dilute the eluate according to the solvent characteristics of the second dimension, thereby avoiding 2D peak distortions; and (iii) adapt the injected amount to the second column according to the relative concentration of the components in a complex sample. However, careful consideration of the system setup is necessary. It is demonstrated how experimental results can be significantly affected in terms of peak broadening and reproducibility if optimization of the interface is not taken into account.In addition, under the optimized conditions, the reproducibility in peak area and dispersion in the 2D dimension were evaluated as a function of the amount of sample transferred in terms of percentage of filled loop.