Objective: The objective of this work was to evaluate the use of sludge generated during the culture of Arapaima gigas as an alternative medium for Nannochloropsis sp. Theoretical Framework: Microalgae are photosynthetic micro-organisms with the ability to transform organic waste or effluents into valuable biomass. They are currently of great interest for their use in the food and pharmaceutical industry. Nannochloropsis sp. is a valuable microalga due to its excellent lipid profile. Method: The sludge used was collected from A. gigas culture ponds at the CITE Acuícola Pesquero Ahuashiyacu (Tarapoto-Peru). An aqueous extract of sludge (EAL) was obtained from the sample by a thermal procedure, which was dosed into the cultures at concentrations of 100, 150 and 200 mL/L. The experimental units followed the traditional batch culture arrangement under laboratory conditions. Guillard F/2 medium was used as control treatment. The characterisation of metals in the medium was performed by coupled plasma induction (ICP) and the determination of ammonium and nitrite by colorimetric tests. In addition, population density, productivity and total lipid content were estimated. Results and Discussion: Chemical analyses revealed high concentrations of calcium, sodium, magnesium, iron, potassium, phosphorus, ammonium and nitrite (655, 648, 283, 67, 32, 17, 1.5, and 10 ppm, respectively). Likewise, the best growth (3590.00 ± 91.24 x 104 cells/mL) and productivity (0.69 ± 0.01 g/L) was obtained with 200 mL/L of EAL, presenting significant statistical differences (p < 0.05) with respect to the other treatments. In contrast, the highest lipid production (29.67 ± 1.53 %) was observed in the control treatment, far exceeding the EAL cultures; however, no significant statistical difference was observed between EAL treatments. Research Implications: These results demonstrate the feasibility of using EAL as an alternative medium for the production of Nannochloropsis sp. with a positive environmental and economic impact on the production of A. gigas. Originality/Value: The use of sludge generated in fish culture, in particular A. Gigas, has not been reported previously. In this study, we demonstrate for the first time the feasibility of using this sludge in the culture of the microalga Nannochloropsis sp. which also has a high potential for use in fish larviculture.
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