High Production Levels Research Articles

Immunocorrective effects of Tricor fenofibrate in the treatment of the early and intermediate stages of age-related macular degeneration

Age-related macular degeneration (AMD) is the leading cause of irreversible visual impairment, blindness and disability in the elderly. Treatment of end-stage AMD is extremely limited and requires invasive interventions. Currently, there is an active search for remedies aimed at preventing the degenerative process in the retina in the early stages of the disease. AMD is a multifactorial pathology, has common mechanisms with diseases associated with aging, metabolic shifts and hemodynamic disorders. The study of the effects of drugs already used to correct these disorders in the general clinic may also be of interest to ophthalmologists. The aim of the work was to study the dynamics of inflammatory markers of activation of the vascular endothelium and cytokines of the immune response in the tear fluid (TF) of patients with initial and intermediate stages of AMD against the background of the use of Tricor fenofibrate. 65 people were examined, divided into three groups according to the AREDS classification: group I – 20 people with early AMD (AREDS2), group II – 16 patients with intermediate AMD (AREDS3), 29 healthy elderly people without ophthalmopathology entered the age control group (AREDS1 – risk group). Patients of groups I and II received Tricor at a dose of 145 mg once a day for 9 months. Tear fluid (TF) was taken twice: before and immediately after the completion of the course of treatment. The determination of sICAM-1, sVCAM-1, sE-, sP-selectin and MCP-1/CCL2 in CS was performed by flow cytometry using a self-constructed multiplex panel from compatible simplex test kits Human FlowCytomixTM Simplex (Bender MedSystem GmbH, Germany); IL-1β, IL-2 IL-6 TNFα was determined within the framework of the Human Flow CytomixTM 15 Flex system (Bender MedSystem GmbH, Germany). Data processing was performed in the FlowCytomix Pro v 6.0 package (Bender Med Systems GmbH, Germany). According to the results of the study, a significant effect of Tricor fenofibrate was found on the initially high levels of local production of IL-1β, IL-2, IL-6, MCP-1/CCL2 and sICAM-1 in the AREDS2 group, which indicated a direct anti-inflammatory, vasoprotective effect in the treatment of the initial stage of AMD; a similar effect of the drug, but less pronounced, noted in the AREDS3 group. The ophthalmological examination data also indicated stabilization of visual functions and the clinical picture of the fundus, improvement of intraocular blood circulation in patients of the main groups. Thus, the use of Tricor can help prevent the degenerative process in the retina in the early stages of AMD development.

An efficient approach for overproduction of DNA polymerase from Pyrococcus furiosus using an optimized autoinduction system in Escherichia coli.

High fidelity DNA polymerase from Pyrococcus furiosus (Pfupol) is an attractive alternative to the highly popular DNA polymerase from Thermus aquaticus. Because this enzyme is in great demand for biotechnological applications, optimizing Pfupol production is essential to supplying the industry's expanding demand. T7-induced promoter expression in Escherichia coli expression systems is used to express recombinant Pfupol; however, this method is not cost-effective. Here, we have effectively developed an optimized process for the autoinduction approach of Pfupol expression in a defined medium. To better examine Pfupol's activities, its purified fraction was used. A 71mg/L of pure Pfupol was effectively produced, resulting in a 2.6-fold increase in protein yield when glucose, glycerol, and lactose were added in a defined medium at concentrations of 0.05%, 1%, and 0.6%, respectively, and the condition for production in a 5 L bioreactor was as follow: 200rpm, 3 vvm, and 10% inoculant. Furthermore, the protein exhibited 1445 U/mg of specific activity when synthesized in its active state. This work presents a high level of Pfupol production, which makes it an economically viable and practically useful approach.

Potential nutrient, carbon and fisheries impacts of large-scale seaweed and shellfish aquaculture in Europe evaluated using operational oceanographic model outputs

Large-scale seaweed and shellfish aquaculture are increasingly being considered by policymakers as a source of food, animal feed and bioproducts for Europe. These aquacultures are generally thought to be low impact or even beneficial for marine ecosystems as they are ‘extractive’ – i.e., growing passively on foodstuff already available in seawater, and with potential habitat provision, fisheries, climate mitigation and eutrophication mitigation benefits. At some scale however, over-extraction of nutrients or chlorophyll could potentially have a negative effect on natural systems. Understanding the likely impacts of aquaculture production at scale is important to identify when safe limits are being approached. Taking seaweed aquaculture as the primary focus, this work uses operational oceanographic model outputs to drive prognostic growth models to predict the likely optimal distribution of seaweed farms across European waters to meet different production scenarios. A novel nutrient transport scheme is then used to model the interacting ‘footprints’ of nutrient drawdown from aquaculture facilities to demonstrate the likely spatial impact of large-scale aquaculture. Evaluation of both seaweed and shellfish contributions to CO2 balance under large scale production, and the potential impact on fisheries are also considered. The study finds that the impact of intensive seaweed aquaculture on nutrient availability could be significant where many farms are placed close together; but at the regional/basin scale even the highest level of production considered does not significantly impact total nutrient budgets. Seaweed aquaculture has the potential to extract large amounts of carbon dioxide, but the impact on carbon budgets depends on the end-use of the extracted seaweed. Shellfish aquaculture is a net source of CO2 due to the impact of calcification of shells on the carbonate system (i.e., alkalinity removal). However, gram-for-gram the CO2 impact of shellfish production is likely to be less than the impact of land-based meat production. Whilst operational oceanographic models are useful for taking a ‘broad brush’ approach to likely placement and impacts of aquaculture, reliable yield predictions for individual locations across European waters would require models integrating more physical and biogeochemical factors (wave environment, local currents, riverine inputs) at a finer scale than currently achievable.

Abstract C079: Exploring acinar to ductal metaplasia in pancreatitis and precancerous contexts using FixNCut

Abstract Acinar to ductal metaplasia (ADM) is the process by which acinar cells transition to a duct-like state acquiring ductal markers and morphology. This process can be driven by pancreas inflammation or injury, such as pancreatitis, where it is thought to be an adaptive response to help mitigate damage and facilitate repair. ADM is also observed following acinar-specific oncogenic KRAS mutations and known to precede PDAC precancerous lesions termed pancreatic intraepithelial neoplasias (PanINs). While ADM is well characterized histologically, cellular and molecular details of ADM and PanIN formation remain less well understood, in part due to high levels of digestive enzyme production in acinar cells that hamper characterization. Leveraging our recently described DSP-fixation method, FixNCut, to improve capture and transcriptome quality of acinar and ADM populations in the pancreas, we characterize ADM arising in three contexts: acute pancreatitis, recurrent acute pancreatitis, and acute pancreatitis with an oncogenic KRAS mutation known to drive the formation of PanINs. FixNCut and single-cell RNA-sequencing enabled us to elucidate the transcriptomic landscape of ADM and define changes to the microenvironment in unprecedented detail. Our findings indicate that the cellular and transcriptional landscape of recurrent acute pancreatitis closely mirrors that of acute pancreatitis for up to 6 weeks (or 6 bouts of 2-day acute pancreatitis), which was the longest timepoint tested. In contrast, acute pancreatitis in the presence of an acinar-specific oncogenic KRAS mutation results in a distinct transcriptional profile for both ADM and the surrounding microenvironment compared to pancreatitis alone. Additionally, we use FixNCut to preserve the epigenomes of acinar cells, enabling us to sort acinar cells and profile their histone modifications in various contexts of ADM. We benchmark and validate our results against existing mouse and human datasets of precancer and cancer. Through this work, we aim to enhance our understanding of ADM in various contexts, providing insights into acinar cell plasticity and early PDAC precursor formation that may inform strategies for early detection and interception. Citation Format: Katherine Aney, Woo-Jeong Jeong, Pal Koak, Isabel Kim, Sahar Nissim. Exploring acinar to ductal metaplasia in pancreatitis and precancerous contexts using FixNCut [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pancreatic Cancer Research; 2024 Sep 15-18; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl_2):Abstract nr C079.

Investigating the Effects of Simultaneous Administration of Melatonin and Atorvastatin against High Glucose-Induced Oxidative Stress and Apoptosis in Cultured Chondrocytes.

Osteoarthritis (OA) is a prevalent joint disorder categorized into phenotypic subtypes, including those associated with age, traumatic events, and metabolic syndrome. In the aging population, type 2 diabetes mellitus (T2DM) and osteoarthritis (OA) frequently coexist. This can result in higher rates of disability and a greater financial burden. This study aimed to investigate the protective effects of melatonin and atorvastatin together against oxidative stress and apoptosis induced by high glucose in C28I2 human chondrocytes. After being pretreated for 6 hours with melatonin (10 and 100 μM) and atorvastatin (0.01 and 0.1 μM), C28I2 cells were exposed to a high concentration of D-glucose (75 mM) for 72 hours. The impact of a high D-glucose concentration (75 mM), with or without melatonin and/or atorvastatin, on cell viability, intra-cellular ROS generation, lipid peroxidation level, antioxidant activities, and the expression of proteins, including Bax, Bcl-2, and caspase-3, was analyzed. Melatonin and atorvastatin combination effectively inhibited high glucose-induced cytotoxicity, ROS production, and MDA and mitochondrial membrane potential levels. The combination of melatonin and atorvastatin was more successful in reducing ROS production compared to each of the drugs alone. Melatonin, but not atorvastatin, reversed high glucose-induced alteration in the catalase activity. Furthermore, the combination of melatonin and atorvastatin significantly enhanced the ability of each medication to lower the expression of pro-apoptotic protein Bax. The combination of melatonin and atorvastatin exerted greater protective effects against hyperglycemia-induced toxicity in chondrocytes.

A study on the performance of corn grain dryer using hybrid solar drying with liquefied petroleum gas

Corn is a widely produced and consumed agricultural product, used as fuel, staple food, and livestock feed. The high level of corn production can lead to potential post-harvest food losses. Drying methods offer a solution to extend the shelf life of a substance. Hybrid solar drying, which combines solar drying with additional heating, particularly using liquefied petroleum gas (LPG), was introduced in this research to ensure faster and more efficient drying processes. The three drying methods compared in this study consist of open sun drying, natural solar drying, and hybrid solar drying. The research results show an average corn grain moisture content of 13.95% over 10.5 hrs, 13.75% over 6 hrs, 13.65% over 7 hrs, 13.45% over 5 hrs, and 13.65% over 4.5 hrs in open sun drying, natural solar drying, and hybrid solar drying with temperature variables of 40°C, 50°C, and 60°C. The average drying rates were 0.212 g/min, 0.364 g/min, 0.318 g/min, 0.439 g/ min, and 0.477 g/min, respectively. The average drying efficiency values for each variable were 2.968%, 4.719%, 5.358%, 5.271%, and 6.037%. In the proximate test, the corn grain moisture content in the hybrid solar drying variable at 60°C was 13.8%, ash was 1.24%, protein was 7.76%, fat was 4.40%, and carbohydrate was 72.8%. For the corn grain color test, the average values obtained were L = 46.48, a = 4.58, and b = 25.37. Meanwhile, the aflatoxin test for dried corn grains showed values of aflatoxin B1, B2, G1, and G2 <0.5 with a total aflatoxin content <2.00. Therefore, hybrid solar drying is recommended over open sun drying and natural solar drying because of its ability to operate even in unfavorable weather conditions, its temperature control capability, its ability to accelerate the drying process, and its capacity to maintain the quality of the material.

Biofilm-forming bacteria associated with corals secrete melanin with UV-absorption properties.

Corals are colonized by a plethora of microorganisms, and their diversity plays a significant role in the health and resilience of corals when they face oxidative stress leading to bleaching. In the current study, we examined 238 bacteria isolated from five different coral species (Acropora hyacinthus, Pocillopora damicornis, Podabacea crustacea, Porites lobata, and Pavona venosa) collected from the coral reef ecosystems of Kavaratti, Lakshadweep Islands, India. We found that bacteria such as Psychrobacter sp., Halomonas sp., Kushneria sp., Staphylococcus sp., Bacillus sp., Brachybacterium sp., Citrobacter sp., and Salinicola sp. were commonly present in the corals. On the other hand, Qipengyuania sp., Faucicola sp., Marihabitans sp., Azomonas sp., Atlantibacter sp., Cedecea sp., Krasalinikoviella sp., and Aidingimonas sp. were not previously reported from the corals. Among the bacterial isolates, a significant number showed high levels of biofilm formation (118), UV absorption (119), and melanin production (127). Considering these properties, we have identified a combination of seven bacteria from the genera Halomonas sp., Psychrobacter sp., Krasalinikoviella sp., and Micrococcus sp. as a potential probiotic consortium for protecting corals from oxidative stress. Overall, this study provides valuable insights into the coral microbiome and opens up possibilities for microbiome-based interventions to protect these crucial ecosystems in the face of global environmental challenges.

Geranylgeraniol: Bio-based platform for teprenone, menaquinone-4, and α-tocotrienol synthesis

By utilizing the conformational selectivity of biosynthesis and the flexibility of chemical synthesis, researchers have formulated metabolic engineering-based semi-synthetic approaches that initiate with the final product’s structure and identify key biosynthesis intermediates. Nonetheless, these tailored semi-synthetic routes focused on end-products, neglecting the possibility of biobased intermediates as a platform for derivatization. To address this challenge, this studyproposed a novel strategy resembling chemosynthesis-style divergent exploration to amplify the significance of biobased intermediates, in the case of geranylgeraniol (GGOH). Using the novel bifunctional terpene synthase PTTC066 and systematic metabolic engineering modifications, the engineered yeast straindemonstrated high GGOH production levels (3.32 g/L, 0.039 g/L/h). This platformenabled the semi-synthesis of various pharmaceuticals, including the anti-ulcer drug teprenone, the osteoporosis treatment drug menaquinone-4, and introduced a novel route for synthesizingα-tocotrienol. This study offers a fresh outlook on semi-synthetic approaches, opening avenues for improvements, substitutions, and innovations in industrial production processes.

Analyze the Causes of Income Gap Between Urban and Rural Residents: Take Guangdong Province in China as an Example

In nearly 40 years since China's reform and opening-up, Guangdong province has experienced continuous economic growth, reaching high levels of production. Urban and rural development has also seen gradual growth, but the income gap between urban and rural residents has gradually increased. In the initial phase of reform and opening-up, rural and urban development was not adequately supported through limited policies and financial assistance for farmers. As Restructuring and liberalizing policies continued, China's economic reconstruction made significant progress, and the income gap between urban and rural areas deepened. With the advancement of China's economic level, Guangdong province has experienced increased economic development, but the income disparity between urban and rural areas has continued to grow gradually. However, in recent years, the income gap between rural inhabitant and countryside resident in Guangdong province has not increased at the same rate as in the past, although this gap persists. This paper analyzes the per individual expendable income for urban and countryside inhabitant in Guangdong province from 2010 to 2020 as an indicator.

Community gardens support high levels of food production, but benefit distribution is uneven across the gardener community

AbstractUrban community gardens are important social–ecological systems from which urban citizens receive many benefits. In this study of 18 urban community gardens in the Central Coast of California, USA, we use a combination of gardener surveys and field-based measurements to evaluate the amount of fresh fruit and vegetables produced by gardeners. We then investigate how food production differed between segments of the gardening population, specifically as a function of gardening experience, time spent in gardens, and food security status. Lastly, we ask gardeners to describe their motivations for gardening to better understand how motivations may relate to individual levels of food production. Thirty-eight percent of gardeners estimate harvesting one to five pounds of food per week, with another 26% estimating six to ten pounds. These estimates were corroborated by field measurements of tomato, squash, and pepper cultivation, where gardeners produced, at the height of the harvest season, an average of four pounds of food per week—an estimated savings of ~ $16USD per week (compared to the cost of local organic fruits and vegetables, June 2023 prices). Regarding the ability of community gardens to reduce food insecurity, gardeners who spent more time in the garden and with higher incomes reported higher food security, while those with larger families or lower incomes were more food insecure. These results show that gardeners in most need of food support were not necessarily the ones cultivating the most fruits and vegetables. While 48% of gardeners reported food cultivation as a primary motivator for gardening, many other motivations (e.g., hobby, being outdoors, relaxation, social interaction, and exercise) were identified as reasons to spend time in the garden, indicating that food production is not the only factor motivating gardeners. Overall, we document that community gardens can be highly productive and provide valuable produce that substantially offsets high fresh food costs; however, gardeners with the greatest food needs are currently not the largest producers, but could benefit from additional resources and support.

Liver Biopsy Technique for Analysis of Hepatic Content during Pregnancy and Early Lactation in Dairy Goats.

Biopsy techniques in dairy goats are currently limited. This study aimed to describe a liver biopsy technique in dairy goats and to evaluate liver triglyceride levels and glycogen content. Sixty-nine dairy goats in the final stage of pregnancy and early lactation period were selected. Fifty goats were selected randomly for hepatic biopsy (HB) according to gestational period and were characterized according to fetus number (single: n = 16, multiple: n = 34), supplementation with propylene glycol (diet: n = 23, diet+PG: n = 27), and milk production levels (high: 3.0 ± 0.4 L/day, n = 15; low: 1.4 ± 0.4 L/day, n = 26). Liver tissue samples were obtained through biopsy on days -30, -20, -15, -10, -5, and 15 days after calving. Hepatic triglyceride and glycogen were quantified. The results were analyzed using the F-test at a 5% significance level and a comparison of means using the Tukey test. The liver biopsies did not influence dry matter intake, body weight, or milk yield. Hepatic glycogen concentration was lower 15 days after calving than it was prior to calving, except on day -20. Goats that generated high levels of milk production had lower triglyceride levels than goats that generated low levels of milk production. The biopsy technique is a safe method for obtaining tissue and evaluating liver content in dairy goats. The milk production level and days relative to parturition influence the hepatic triglyceride and glycogen content in dairy goats.

Have a Little Heart (or Not): Highly Minimized Skeletal Muscle Regulatory Cassettes with Low or No Activity in the Heart.

Adeno-associated virus-mediated gene therapies for certain muscle disorders require regulatory cassettes that provide high-level, striated muscle-specific activity. However, cardiotoxicity has emerged as a serious concern in clinical trials for Duchenne muscular dystrophy and X-linked myotubular myopathy. While this may be caused by systemic inflammatory effects of the treatment, high transgene expression in the heart may also play a role. Thus, certain muscle disorders may require a modulated level of therapeutic expression in the heart, while others may not require any cardiac expression at all. Additionally, the size of some cargos requires regulatory cassettes to be small enough that large cDNAs and other therapeutic payloads can be accommodated. Thus, we have performed enhancer/promoter optimization to develop highly minimized regulatory cassettes that are active in skeletal muscles, with either low or no detectable activity in cardiac muscle. Our No-heart (NH) cassette is active in most skeletal muscles, but exhibits only very low activity in extensor digitorum longus (EDL), soleus, and diaphragm, and no activity in the heart. By contrast, our Have a Little Heart (HLH) cassette displays high activity in most skeletal muscles, comparable to the ∼800-bp CK8 cassette, with increased activity in EDL, soleus, and diaphragm, and low activity in the heart. Due to their small size, these cassettes can be used in therapeutic strategies with both flexible (e.g., antisense) and stringent (e.g., CRISPR/Cas or bicistronic) size limitations. Thus, our new cassettes may be useful for gene therapies of muscle disorders in which the need for low or almost no expression in cardiac muscle would outweigh the need for high levels of therapeutic product in certain skeletal muscles.

Solar energy feasibility for power generation in lower shire districts: A case study of Chikwawa District

Background: Electricity availability is an essential part of both economic and non-economic activities in any country. Malawi being one of the developing nations experiences a lot of challenges both in its economic and non-economic activities due to unlimited levels of electric supply in its country. This inadequacy of limited supply has remained a challenge for a longer period of time to the effect that the country Malawi for a longer period of time has an overwhelming record of blackouts and load shedding. Consequently, this has affected in a negative way the economic activities of this nation such as low productivity in the production of goods and services making livelihood a difficult. Similarly, noneconomic activities such social interactions seem to be challenging due to lack of electricity. In this regard an investigation in determining new innovations towards electricity production remained one of the most important aspects in this county of Malawi. Method: This study was conducted in Chikwawa district, Malawi, to assess the feasibility of solar energy production. The methods used include temporal variability, correlation, trend, and spatial analysis to assess solar radiation, sunshine hours, and their distribution in the area. Result: As a result, the study was established with the purpose to assess feasible regions within the country of Malawi to innovate solar energy production to boost high levels of solar energy production. This study was conducted in the lower shire district and to be specific in Chikwawa district. The objectives of the feasibility in Chikwawa district included; assessing solar radiation and sunshine hours towards solar energy production, the spatial distribution of solar radiation and sunshine hours towards solar energy production, the trend of sunshine and solar radiation towards solar production and at the end to map the areas in Chikwawa District where solar energy production could be harnessed. Through the use of temporal variability, correlation analysis, trend analysis, and spatial analysis, the findings of this investigation established that harnessing solar energy is much more possible in areas of the lower shire which is the among others include Chikwawa district of Malawi as most areas currently in this region has less places with solar energy production but with high availability of solar radiation. Conclusion: For the fact that the findings reveal more feasible places to establish solar energy production, this study recommends commitment by government and private sector to greatly establish solar energy production units in this area whilst focusing on identifying other potential areas where solar energy production can be harnessed in Malawi. In this regard, this exposition has achieved its purpose of fostering renewable energy production through innovating and investing in solar energy production. Novelty/Originality of this study: This detailed spatial and temporal analysis study has identified optimal locations for solar energy production, providing a new approach to renewable energy planning in developing countries.

Development of a cost-effective medium suitable for the growth and fucoxanthin production of the microalgae Odontella aurita using jeju lava seawater and agricultural fertilizers

Diatoms have garnered significant attention in various industries, including feed, food, and pharmaceuticals, due to their potential as advanced biomaterials containing valuable components such as fatty acids, pigments, and nanoparticles. However, the industrial-scale cultivation of diatoms has faced challenges such as limited productivity and high production costs. This study aimed to develop a cost-effective growth medium for the cultivation of Odontella aurita, a diatom known for its high levels of fatty acids and fucoxanthin production. The research investigated the impact of Jeju lava seawater on the growth of O. aurita OAOSH22 and evaluated the effectiveness of five different agricultural fertilizers in cultivation. The final composition included the selected fertilizer supplemented with silicate. The growth of O. aurita in F/2 medium supplemented with Jeju lava seawater was found to be 1.8 times higher than in the ordinary seawater-based F/2 medium, attributed to the elevated silicon content in Jeju lava seawater. The optimized medium, incorporating Jeju lava seawater and agricultural fertilizer, resulted in a significant increase in biomass by a factor of 15.6 and a substantial rise in fucoxanthin production by a factor of 7.5. Additionally, the utilization of this optimized medium resulted in a significant reduction of 95.1 % and 89.7 % in the costs associated with biomass and fucoxanthin production, respectively. These findings suggest that the use of Jeju lava seawater promotes the growth of O. aurita, and the optimized medium, including agricultural fertilizers, provides a cost-effective alternative to existing microalgal media.

An Examination of Technical Efficiency of MSMEs Industries in Haryana: A Case Study of Faridabad District

India's economy's micro, small, and medium-sized enterprises (MSME) sector produce several goods. One of the most accurate ways to characterize the Industrial Sector is by its technical efficiency, which allows it to sustain a high level of manufacturing production. An investigation of the productivity and efficiency of micro, small, and medium-sized firms (MSMEs) in the industrial sector of Faridabad, Haryana, is carried out using parametric frontier analysis. Within the context of his essay on efficiency evaluation, which was published in 1957, Farrell presented various approaches for examining productivity and efficiency. The function that determines the creation of the stochastic frontier. We use a multistage estimation technique that incorporates four distinct error components to arrive at reasonable estimates. From 2016 through 2022, the data from the Ministry of MSMEs and the organization that is part of the state government were utilized. An investigation into the machinery and equipment that is utilized by micro, small, and medium-sized firms will be carried out as part of this study.

Dampak Perubahan Harga Beras terhadap Kesejahteraan Petani

The high level of agricultural production in Solok Regency has not been in line with the increase in farmers' welfare. In fact, the level of farmer welfare is an important factor in the development of the agricultural sector. The aim of this research is to determine the impact of changes in rice prices on farmer welfare using a case study approach. Data collection techniques include interviews, observation and documentation. For technical data analysis through percentage analysis. This research revealed that 59.2% of farmers before the increase in rice prices had a good economic condition, however, when the price of rice increased, the economic condition of rice farmers became less good, amounting to 66.3%. Apart from that, some farmers stated that their income increased by a percentage of 9.2%. The increase in rice prices does not result in the consumption patterns of farming communities decreasing, farmers reduce rice consumption and use other foods that contain carbohydrates as substitute foods.

Shale pore characteristics and their impact on the gas-bearing properties of the Longmaxi Formation in the Luzhou area

Deep shale has the characteristics of large burial depth, rapid changes in reservoir properties, complex pore types and structures, and unstable production. The whole-rock X-ray diffraction (XRD) analysis, reservoir physical property parameter testing, scanning electron microscopy (SEM) analysis, high-pressure mercury intrusion testing, CO2 adsorption experimentation, and low-temperature nitrogen adsorption–desorption testing were performed to study the pore structure characteristics of marine shale reservoirs in the southern Sichuan Basin. The results show that the deep shale of the Wufeng Formation Longyi1 sub-member in the Luzhou area is superior to that of the Weiyuan area in terms of factors controlling shale gas enrichment, such as organic matter abundance, physical properties, gas-bearing properties, and shale reservoir thickness. SEM is utilized to identify six types of pores (mainly organic matter pores). The porosities of the pyrobitumen pores reach 21.04–31.65%, while the porosities of the solid kerogen pores, siliceous mineral dissolution pores, and carbonate dissolution pores are low at 0.48–1.80%. The pores of shale reservoirs are mainly micropores and mesopores, with a small amount of macropores. The total pore volume ranges from 22.0 to 36.40 μL/g, with an average of 27.46 μL/g, the total pore specific surface area ranges from 34.27 to 50.39 m2/g, with an average of 41.12 m2/g. The pore volume and specific surface area of deep shale gas are positively correlated with TOC content, siliceous minerals, and clay minerals. The key period for shale gas enrichment, which matches the evolution process of shale hydrocarbon generation, reservoir capacity, and direct and indirect cap rocks, is from the Middle to Late Triassic to the present. Areas with late structural uplift, small uplift amplitude, and high formation pressure coefficient characteristics favor preserving shale gas with high gas content and production levels.

The contribution of animal antibiotic use to antibiotic resistance in human infections: Panel evidence from Denmark

Antibiotic use (ABU) in animals is postulated to be a major contributor to selection of antibiotic resistance (ABR) which subsequently causes infections in human populations. However, there are few quantifications of the size of this association. Denmark, as a country with high levels of pig production and strong ABR surveillance data, is an ideal case study for exploring this association.This study compiles a dataset on ABU across several animal species and antibiotic classes, and data on the rate of antibiotic resistance (ABR) in humans across key pathogens, in Denmark over time (2010−2020). Panel data regressions (fixed effects, random effects, first difference and pooled ordinary least squares) were used to test the association between the level of ABR in human isolates and the level of ABU in animals.A positive relationship was identified between ABR in humans and ABU in cattle, with some evidence of a positive relationship for poultry and companion animals, and a negative relationship for fish, although the latter is likely driven by confounding factors. When lagging ABU by one year, the effect of ABU in cattle and companion animals remained similar, the effect of ABU in poultry fell in size, and ABU in fish was no longer significant, perhaps due to differences in life cycle length among animal species. Additional covariates were explored, including pet populations, agricultural production and GDP per capita (at purchasing power parity), but these results were limited by the statistical power of the dataset. Under all models, animal ABU determined only a minority of the change in human ABR levels in this context with adjusted R2 ranging from 0.19 to 0.44.This paper supports the role of animal ABU in determining human ABR levels but suggests that, despite comprising a large portion of systemwide ABU, it only explains a minority of the variation. This is likely driven in part by data limitations, and could also be due to a persistence of ABR once resistance has emerged, suggesting a significant role for socioeconomic and transmission factors in bringing ABR down to desirable levels.

Biotic and abiotic processes in Ediacaran spheroid formation

Organic-rich shales from the uppermost Doushantuo Fm. (South China) record one of the most negative carbonate carbon isotopic excursions in Earth’s history, known as the Shuram excursion, and contain meter to micro-size spheroids. In this study, we use Raman and energy dispersive spectroscopy to identify and describe the most common diagenetic spheroids to refine our understanding of the profound perturbations of the carbon cycle and the evolution of pore fluid chemistry imprinted in the sedimentary Precambrian record, especially in the late Ediacaran. The presence of 13C-depleted carbonate concretions or organic matter (OM) enclosed by lenticular dolomitic structures within the host shale unit suggests OM remineralisation and anaerobic oxidation, resulting in authigenic carbonate precipitation during the earliest stages of sediment diagenesis. Other mineralogical features, however, point to high levels of primary production, such as apatite bands that host spheroidal microfossils with highly fluorescent quartz and OM within abiotic concretions. These observations highlight the importance of considering co-occurring biotic and abiotic processes in explaining the formation of diagenetic spheroids in ancient sedimentary environments. From an astrobiology perspective, the interplay of biotic and abiotic processes reflects the complexity of early life systems and the environments that may exist on other terrestrial planets. Understanding the signatures of biotic and abiotic interactions in the Doushantuo Fm. is crucial for identifying potential biosignatures in extraterrestrial materials, thereby enhancing our understanding of life’s universality and adaptability in diverse and extreme environments.

Meat quality and safety issues during high temperatures and cutting-edge technologies to mitigate the scenario.

Climate change, driven by the natural process of global warming, is a worldwide issue of significant concern because of its adverse effects on livestock output. The increasing trend of environmental temperature surging has drastically affected meat production and meat product quality, hence result in economic losses for the worldwide livestock business. Due to the increasing greenhouse gas emissions, the situation would get prolonged, and heat exposure-related stress is expected to worsen. Heat exposure causes metabolic and physiological disruptions in livestock. Ruminants and monogastric animals are very sensitive to heat stress due to their rate of metabolism, development, and higher production levels. Before slaughter, intense hot weather triggers muscle glycogen breakdown, producing pale, mushy, and exudative meat with less water-holding capacity. Animals exposed to prolonged high temperatures experience a decrease in their muscle glycogen reserves, producing dry, dark, and complex meat with elevated final pH and increased water-holding capacity. Furthermore, heat stress also causes oxidative stresses, especially secondary metabolites from lipid oxidation, severely affects the functionality of proteins, oxidation of proteins, decreasing shelf life, and food safety by promoting exfoliation and bacterial growth. Addressing the heat-related issues to retain the sustainability of the meat sector is an essential task that deserves an inclusive and comprehensive approach. Considering the intensity of the heat stress effects, this review has been designed primarily to examine the consequences of hot environment temperatures and related stresses on the quality and safety of meat and secondarily focus on cutting edge technology to reduce or alleviate the situational impact.