Background: Wearing a face mask became a part of daily life due to the COVID-19 pandemic and fine particulate matter (PM2.5) air pollution in Thailand. However, performing activities while wearing different types of masks can cause different physiological effects, especially in the pre-aging population that still performs outdoor activities regularly. Objectives: To compare the impacts of different types of the mask (no mask, N95, surgical, cloth, PM2.5 mask) on dyspnea, breathing effort, blood oxygen saturation (SpO2), and functional capacity (six-minute walk distance and estimated VO2max) during the six-minute walk test (6MWT) in pre-aging individuals. Materials and methods: Twenty-four healthy pre-aging individuals (54±3 years) performed five trials of 6MWT wearing different types of masks. Pre-test and post-test of dyspnea, breathing effort, and blood oxygen saturation values were recorded. The estimated VO2max was calculated from 6MWT. The satisfaction for each type of mask was collected using questionnaires. Results: SpO2, walking distance, and estimated VO2max were not significantly different (p>0.05) among all trials. Changes in dyspnea, breathing effort, and systolic blood pressure values were statistically different (p=0.026, p=0.031, and p=0.028, respectively). Despite post hoc analysis revealing a nonsignificant difference in delta changes in dyspnea between walking trials, there was a clinical difference in dyspnea while wearing N95 compared to no mask as well as surgical masks compared to other masks (MCID for Borg scale ≥1). Wearing an N95 or surgical mask resulted in higher dyspnea. Moreover, wearing an N95 mask also resulted in higher breathing effort and systolic blood pressure compared to wearing no mask. Conclusion: Wearing various types of masks in the pre-aging population during moderate-intensity activity did not result in differences in oxygen saturation and functional capacity. Wearing an N95 resulted in higher levels of dyspnea, breathing effort and systolic blood pressure than wearing no mask. Wearing a surgical mask may have resulted in higher levels of dyspnea than wearing no mask, a cloth mask, or a PM2.5 mask.