High Influent Concentrations Research Articles

Biological degradation of organic micropollutants in GAC filters–temporal development and spatial variations

The capacity for organic micropollutant removal in granular activated carbon (GAC) filters for wastewater treatment changes over time. These changes are in general attributed to changes in adsorption, but may in some cases also be affected by biological degradation. Knowledge on the degradation of organic micropollutants, however, is scarce. In this work, the degradation of micropollutants in several full-scale GAC and sand filters was investigated through incubation experiments over a period of three years, using 14C-labeled organic micropollutants with different susceptibilities to biological degradation (ibuprofen, diclofenac, and carbamazepine), with parallel 16S rRNA gene sequencing. The results showed that the degradation of diclofenac and ibuprofen in GAC filters increased with increasing numbers of bed volumes when free oxygen was available in the filter, while variations over filter depth were limited. Despite relatively large differences in bacterial composition between filters, a degradation of diclofenac was consistently observed for the GAC filters that had been operated with high influent oxygen concentration (DO >8 mg/L). The results of this comprehensive experimental work provide an increased understanding of the interactions between microbial composition, filter material, and oxygen availability in the biological degradation of organic micropollutants in GAC filters.

Effects of High Pharmaceutical Concentrations in Domestic Wastewater on Membrane Bioreactor Treatment Systems: Performance and Microbial Community.

Despite pharmaceuticals being widely detected in water-bodies worldwide, what remain unclear are the effects of high pharmaceutical concentrations on the treatment efficiency of biological wastewater treatment processes, such as membrane bioreactor (MBR) systems. This study investigated the efficiency of MBR technology in the treatment of synthetic wastewater containing a mixture of five typical pharmaceuticals (ofloxacin, sulfamethoxazole, sulfamethylthiadiazole, carbamazepine and naproxen) with a total concentration of 500 µg/L. Both the control MBR (MBRc) without pharmaceutical dosing and the MBR operated with high influent pharmaceutical concentrations (MBRe) were operated under room temperature with the same hydraulic retention time of 11 h and the same sludge retention time of 30 d. The removal efficiency rates of total nitrogen and total phosphorus were 83.2% vs. 90.1% and 72.6% vs. 57.8% in the MBRc vs. MBRe systems, and both MBRs achieved >98% removal of organics for a 180-day period. The floc size decreased, and membrane fouling became more severe in the MBRe system. Microbial diversity increased in the MBRe system and the relative abundances of functional microbe differed between the two MBRs. Furthermore, the total relative abundances of genes involved in glycolysis, assimilating nitrate reduction and nitrification processes increased in the MBRe system, which could account for the higher organics and nitrogen removal performance. This work provides insights for MBR operation in wastewater treatment with high pharmaceutical concentrations.

DOM accumulation in the hyporheic zone promotes geogenic Fe mobility: A laboratory column study

Hyporheic zone (HZ) systems have a natural purification capacity, and they are commonly used to provide high quality drinking water. However, the presence of organic contaminants in HZ systems in anaerobic environments causes the aquifer sediments to release metals (e.g., Fe) at levels above drinking water standards, which affects the quality of groundwater. In this study, the effects of typical organic pollutants (dissolved organic matter (DOM)) on Fe release from anaerobic HZ sediments were investigated. Ultraviolet fluorescence spectroscopy, three-dimensional excitation-emission matrix fluorescence spectroscopy, excitation–emission matrix spectroscopy coupled with parallel factor analysis and Illumina MiSeq high-throughput sequencing were used to determine the effects of the system conditions on Fe release from HZ sediments. Compared with the control conditions (low traffic and low DOM as a baseline), the Fe release capacity was enhanced by 26.7 % and 64.4 % at low flow rate (85.8 m/d) and high organic matter concentration (1200 mg/L), which was consistent with the residence-time effect. The transport of heavy metals under different system conditions varied with the influent organic composition. The influent organic matter composition and fluorescence parameters (the humification index, biological index and fluorescence index) were closely related to the release of the Fe effluent, while these factors had less influence on Mn and As. From 16S rRNA analysis of the aquifer media at different depths at the end of the experiment, under low flow rate and high influent concentration conditions, reduction of Fe minerals by Proteobacteria, Actinobacteriota, Bacillus, and Acidobacteria promoted the release of Fe. These functional microbes play an active role in the Fe biogeochemical cycle in addition to reducing Fe minerals to promote Fe release. In summary, this study reveals the effects of the flow rate and influent DOM concentration on the release and biogeochemistry of Fe in the HZ. The results presented herein will contribute to a better understanding of the release and transport of common groundwater contaminants in the HZ and other groundwater recharge environments.

The significance of fluorinated compound chain length, treatment technology, and influent composition on per- and polyfluoroalkyl substances removal in worldwide wastewater treatment plants.

Wastewater treatment plants (WWTPs) are deemed major conveyors and point sources of per- and polyfluoroalkyl substances (PFAS) to the environment. This statistical meta-analysis of existing literature from the past 15 years focused on the significance of treatment type for PFAS removal efficiencies and the influence of PFAS sources (domestic vs. industrial) on their removal. Different sampling events, WWTPs across the world, different treatment technologies, configurations, and processes, as well as different PFAS classes and compounds were considered. This study evaluated 13 PFAS analyzed predominantly in 161 WWTPs across the world. The statistical test results revealed that these 13 frequently detected and reported PFAS can be divided into four groups based on their behavior during wastewater treatment, namely (1) C6-10 perfluorocarboxylic acids (PFCAs), (2) C4,5,11,12 PFCAs, (3) C4,6,8 perfluoroalkane sulfonic acids (PFSAs), and (4) C10 PFSA. In this study, biological treatments such as (1) membrane bioreactors, (2) combination of two or more biological treatments, and (3) biofilm processes revealed the highest PFAS removals, although the addition of a tertiary treatment actually had a nonbeneficial effect on PFAS removal. Moreover, a strong statistical correlation was observed between industrial wastewater sources and the presence of high influent PFAS concentrations in the receiving WWTPs. This indicates that industrial sources were the main contributors of the PFAS load in the analyzed WWTPs. Integr Environ Assess Manag 2024;20:59-69. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

A novel filter-type constructed wetland for secondary effluent treatment: Performance and its microbial mechanism

A novel filter-type constructed wetland was constructed by combining plastic fillers and mineral fillers for secondary effluent treatment. Findings showed that TN, TP and COD removal in the constructed wetland with composite fillers (CFCW) was 3.9%, 8.0% and 3.5% higher than that of constructed wetland with ordinary gravel fillers (CW) in the stable phase, respectively. CFCW showed better pollutants removal when dealing with higher influent concentrations and hydraulic loading. The main functional bacteria in two systems were significantly different (p < 0.05). Composite fillers could change the dominant genera, enhance genera activity and increase genera quantity. Denitrification (e.g., Pseudorhodobacter, Zoogloea, Pseudarthrobacter), nitrification (e.g., Devosia, Nitrospira), heterotrophic nitrification-aerobic denitrification (e.g., Paracoccus) and partial denitrification (e.g., g__Simplicispira) in CFCW provided diverse nitrogen metabolism pathways, resulting in higher nitrogen removal. The novel filter-type constructed wetland is suitable for the advanced treatment of sewage treatment plant effluent with enhanced pollutants removal and exuberant microorganisms.

Evaluating seasonal differences in paraben transformation at two different wastewater treatment plants in Texas and comparing parent compound transformation to byproduct formation

Parabens are commonly used preservatives that are weakly estrogenic. Wastewater effluent is the greatest contributor to the spread of parabens into rivers and other surface water. While previous studies indicate parabens are well removed in wastewater treatment by way of transformation, not much is known about the paraben transformation products. This study evaluates paraben transformation and release at two different wastewater treatment plants in Texas. Paraben concentrations were quantified for influent and effluent by season and by year at both treatment plants. Both seasonal and annual transformation rates were compared between the two wastewater treatment plants. Compounds were compared to evaluate differences in transformation rates and to determine if decreases in parent product concentrations are correlated to changes in transformation product concentrations. The study took place over one year and evaluated each season. Spring had higher influent concentrations and transformation rates at treatment plant 1, while summer had higher influent concentrations and transformation rates at treatment plant 2. PHBA was present in greatest amounts in influent and effluent at both sites with average yearly influent concentrations at 223.9 pM at plant 1 and 211.4 pM at plant 2. Transformation rates of parent parabens were greater at plant 1 with concentration of all three shorter chained parabens decreasing by over 50% after treatment. Formation of dichlorinated transformation products were greater at plant 1 with concentrations of Cl2MeP increasing by 1200% after treatment and Cl2EtP increasing by 940%. While shorter chained parabens generally had a greater transformation rate, no correlations were found between decreases in methyl and ethyl parabens and the formation of their respective dichlorinated transformation products.

Enhanced degradation of ibuprofen in an integrated constructed wetland-microbial fuel cell: treatment efficiency, electrochemical characterization, and microbial community dynamics.

Over the past few decades, there has been a growing concern regarding the fate and transport of pharmaceuticals, particularly antibiotics, as emerging contaminants in the environment. It has been proposed that the presence of antibiotics at concentrations typically found in wastewater can impact the dynamics of bacterial populations and facilitate the spread of antibiotic resistance. The efficiency of currently-used wastewater treatment technologies in eliminating pharmaceuticals is often insufficient, resulting in the release of low concentrations of these compounds into the environment. In this study, we addressed these challenges by evaluating how different influent ibuprofen (IBU) concentrations influenced the efficiency of a laboratory-scale, integrated constructed wetland-microbial fuel cell (CW-MFC) system seeded with Eichhornia crassipes, in terms of organic matter removal, electricity generation, and change of bacterial community structure compared to unplanted, sediment MFC (S-MFC) and abiotic S-MFC (AS-MFC). We observed that the addition of IBU (5 mg L-1) resulted in a notable decrease in chemical oxygen demand (COD) and electricity generation, suggesting that high influent IBU concentrations caused partial inhibition for the electroactive microbial community due to its complexity and aromaticity. However, CW-MFC could recover from IBU inhibition after an acclimation period compared to unplanted S-MFC, even though the influent IBU level was increased up to 20 mg L-1, suggesting that plants in CW-MFCs have a beneficial role in relieving the inhibition of anode respiration due to the presence of high levels of IBU; thus, promoting the metabolic activity of the electroactive microbial community. Similarly, IBU removal efficiency for CW-MFC (i.e., 49-62%) was much higher compared to SMFC (i.e., 29-42%), and AS-MFC (i.e., 20-22%) during all experimental phases. In addition, our high throughput sequencing revealed that the high performance of CW-MFCs compared to S-MFC was associated with increasing the relative abundances of several microbial groups that are closely affiliated with anode respiration and organic matter fermentation. In summary, our results show that the CW-MFC system demonstrates suitability for high removal efficiency of IBU and effective electricity generation.

Concurrent recovery of ammonia and phosphate by an electrochemical nutrients recovery system with authigenic acid and base

Strong wastewater containing ammonia and phosphate is anticipated to become potential sources of nutrient elements (N and P). However, high N/P molar ratios in most kinds of strong wastewater make it difficult to realize simultaneous and full recovery of N and P. Thereout, the present study developed an electrochemical nutrients recovery system (ECNRS) with authigenic acid and base to integrate transmembrane chemisorption and hydroxyapatite crystallization for concurrent N and P recoveries from wastewater. Compared with separate N or P recovery, the concurrent recovery of N and P in the ECNRS could obviously enhance N and P recovery efficiencies. Under 4.0 V applied voltage and 12 h operation, the ECNRS recovered 79.2 % of N and 100 % of P, which were higher than those of separated N or P recovery. However, increasing influent N or P concentration would prolonged recovery time for the nutrients, which might be attributed to the enhanced pH buffering of catholyte with high influent concentration. Energy consumption for N recovery was 14.6 kWh/kg N with a flux of 47.3 g NH3-N/(m2·d). Economic costs for N and P recovery via the ECNRS were 1.84 $/kg N and 0.69 $/kg P, respectively. Considering the concurrent N and P recovery via ECNRS, the obtained preliminary profit rate could be over 300 %. The developed system with concurrent N and P recoveries showed a cost-effective advantage.

Enrichment of Ca. Jettenia in sequencing batch reactors operated with low nitrogen loading rate and high influent nitrogen concentration

To apply the anammox processes into the mainstream of domestic wastewater treatment plants, two laboratory-scale sequence batch reactors have been developed and used with two different activated sludges seeded in each sequence batch reactors with gradually increases influent total nitrogen concentrations under low nitrogen loading rates. During 320 days of operation, both sequence batch reactors showed high specific anammox activity (0.68 – 0.75 kgN kg−1VSS d−1) and a nitrogen removal efficiency of 97.50%. To monitor changes in microbial community dynamics during enrichment, high-throughput sequencing analysis was performed. Members taxonomically affiliated with Candidatus Jettenia were markedly enriched and predominant in both sequence batch reactors in response to the increasing influent total nitrogen concentrations. These results suggest that Candidatus Jettenia might be a prominent anammox genus under low nitrogen loading rate with high total nitrogen concentration conditions and could be suitably applied to the mainstream process of domestic wastewater treatment systems.

Start-up strategies to develop aerobic granular sludge and photogranules in sequential batch reactors

In this study, start-up strategies to develop conventional aerobic granular sludge (AGS) and algal aerobic granular sludge (AAGS) (photogranules), were investigated. The granulation experiment was conducted in four sequencing batch reactors (SBR), of which two were conventional SBRs (RC1, RC2) used as control, and two were photo-SBRs (R1, R2). R1 and RC1 were operated with a 40-min feeding during the reactors´ anaerobic cycle period, whereas R2 and RC2 with a 60-min feeding. All the reactors were operated in two phases with a C:N = 4:1 in Phase I and 8:1 in Phase II. In Phase I, AGS in RC1 and RC2 was formed 15 days before the AAGS development in R1 and R2. However, the AAGS generally presented better stability and higher diameter. On the other hand, AGS presented greater abundance of extracellular polymeric substances producing organisms, such as Xanthomonadacea and Rhodocyclaceae. Chemical oxygen demand (COD) and NH4+-N removal efficiencies were similar in all the four reactors of approximately 70% and 60%, respectively. In this phase, despite the good biomass structure, the reactors were not able to completely oxidize the high influent concentration of NH4+-N (100 mg.L−1) and COD (400 mg.L−1). This can be associated to the short time of the aerobic phase and low biomass content. In Phase II in all the reactors, a good increase in COD and NH4+-N removal efficiencies to values above 95% and 93%, respectively, was achieved under a higher C:N ratio of 8 with lower influent concentration of NH4+-N (50 mg.L−1). The 60-min anaerobic feeding period in R2 and RC2 resulted in greater removal efficiency of nitrogen, confirming that small variation on cycle periods can affect the biomass composition; the biomass presented more compact granules and larger diameters under 60 min-feeding when compared with those obtained with 40 min-feeding in Phase I.

Enrichment Evaluation of Heavy Metals from Stormwater Runoff to Soil and Shrubs in Bioretention Facilities

Bioretention facilities with different inflow concentrations, growing media and plants were examined to determine whether the soil in these facilities was polluted with heavy metals and whether runoff had obvious toxic effects on plants. Using Beijing soil background value as the standard, the soils were evaluated by bioaccumulation index and single factor index. The results show that stormwater runoff containing Cu caused slight pollution in soils, and stormwater runoff containing Zn and Pb was not polluted. Nemerow comprehensive index evaluation revealed that the heavy metals content in the facilities containing vermiculite (a yellow or brown mineral found as an alteration product of mica and other minerals, used for insulation or as a moisture-retentive medium for growing plants) and perlite (a form of obsidian characterized by spherulites formed by cracking of the volcanic glass during cooling, used as insulation or in plant growth media) were higher than the standard. High influent concentration caused significantly higher heavy metals content in plants. While Pb accumulation in the two studied plants was the highest, Cu and Zn accumulation, which are essential for plant growth, was relatively low. The contents of the three heavy metals in the studied plants also exceeded their corresponding critical values.

Removal of azimsulfuron and zoxamide using a tapered variable diameter biological fluidized bed combined with electrochemistry: Mass fraction division, energy metabolism activity and carbon emissions

The performance of the combination system of tapered variable diameter biological fluidized bed (TVDBFB) with electrochemistry (EC) was evaluated for removing azimsulfuron and zoxamide under different temperatures and influent concentrations. Maximum removal efficiency of azimsulfuron and zoxamide could reach 94% and 98% under higher influent concentration (about 780 mg/L). As temperature decreased from 32 ℃ to 8 ℃, the mSe increased from 48% to 56%, and the mSo and mSxv decreased from 30% to 22% and 27% to 24%, respectively. As the influent COD equivalent concentration of azimsulfuron and zoxamide enhanced from 260 mg/L to 780 mg/L, the Kd increased from 0.06 d-1 to 0.23 d-1. Temperature and influent concentration were main influencing factors of DHA, ATP and ETS. Increasing aeration in TVDBFB and HRT in EC under shock conditions could improve azimsulfuron and zoxamide removal efficiency, however, it was also accompanied by higher carbon emissions.

Impacts of bioreactor operating parameters on removal efficiency, biodegradation rate, molecular distribution, and toxicity of commercial naphthenic acids.

Effects of naphthenic acids (NAs) concentration (50-200mg NA L-1; 35-140mg TOC L-1) and loading rate (1.4-1249mg NA L-1h-1; 1-874mg TOC L-1h-1) on removal efficiency, removal rate, and molecular distribution of NAs, and effluent toxicity were evaluated for biodegradation of commercial NAs mixture in circulating packed bed bioreactors (CPBBs). Increase of NAs concentration and loading rate (shorter residence times) increased the removal rate, while removal efficiency initially declined and then stabilized. The maximum biodegradation rates for 50, 100, 150, and 200mg NA L-1 were 128.0, 321.7, 430.2, and 630.0mg TOC L-1h-1 at loading rates of 218.5, 455.6, 673.5 and 874.0mg TOC L-1h-1, respectively, with removal efficiencies of 58.6, 70.6, 63.9 and 72.1%. Analysis of influent and treated effluents with gas chromatography-mass spectrometry showed that molecular weight and cyclicity (C and Z numbers) affected the biodegradation, with low molecular weight acyclic NAs (C = 6-12) were the most amenable to biodegradation and those with intermediate and high molecular weights (C = 13-22) and moderate cyclicity (Z = -4, -6) were the most recalcitrant. In the biofilm, Proteobacteria and Actinobacteria were the most abundant phyla, and Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria were the dominant classes. Toxicity analyses with Artemia salina and Vibrio fischeri (Microtox) showed that high influent concentrations and loading rates (short residence times) led to higher NAs residual concentration and effluent toxicity. To design and operate large-scale CPBBs, intermediate loading rates and residence times that result in high removal efficiency, reasonable removal rates, and low toxicity are recommended.

Effects of Influent Concentration and Flow Rate on the Sorption of Diesel Molecules on Kapok Fibers at Dynamic Conditions

Kapok fibers were used as a filtering medium in a column-type filtration set-up to separate diesel from water molecules in dynamic conditions. The amount of diesel flowing out the filtration system with respect to time was monitored. The times wherein the diesel first came out the filtering system (breakthrough time) were shorter at higher influent concentration and faster flow rate. Meanwhile, the total sorbed diesel molecules in the filtering system were increasing with the influent concentration while invariant with flow rate. The shorter breakthrough time was associated with the higher amount of diesel molecules that could be sorbed at a shorter time and the rate at which the overall processes of sorption-desorption-resorption proceeded. On the other hand, the sorption capacity of the system was viewed to be affected by the amount of moving diesel molecules that would interact with the kapok fibers and/or surface-sorbed diesel molecules but not by the contact time.

Biological activated carbon filter for greywater post-treatment: Long-term TOC removal with adsorption and biodegradation

Biological activated carbon (BAC) filters can be used to remove residual total organic carbon (TOC) from greywater after a membrane bioreactor. The two main TOC removal processes are adsorption to the granular activated carbon (GAC) and biological degradation. Biodegradation leads to the growth of microorganisms in the filter bed, which can lead to increased pressure loss over the filter bed. However, the roles of sorption and biodegradation in long-term TOC removal and how they complement each other are unclear. We monitored TOC removal from greywater in a BAC filter installed following a membrane bioreactor over more than 900 days. Removal performance depended on the operational time of the BAC filter, the influent TOC concentration, and in the upper part of the filter on the empty bed contact time (EBCT). Across the overall filter, the EBCT did not significantly influence TOC removal, showing that the filter was sufficiently large for the range of flow rates observed. Analysis of the long-term data revealed the equal importance of sorption and biodegradation over the whole operation period and the whole filter bed. Most of the TOC was removed in the upper part of the filter, where biodegradation was the dominant mechanism. In the lower part of the filter, sorption capacity remained and allowed high influent TOC concentrations to be buffered. The generous filter design with low average filtration rates ensured long-term TOC removal. The only maintenance needed was backwashing, which was required only after more than 800 days of operation. Backwashing effectively reduced the pressure loss but had no significant influence on the effluent water quality. Our study shows that BAC filters are a suitable post-treatment step for the treatment of greywater with highly variable flow and TOC concentrations.

Groundwater pollution containing ammonium, iron and manganese in a riverbank filtration system: Effects of dynamic geochemical conditions and microbial responses.

Bench-scale experiments were conducted to investigate the effect of hydraulic loadings and influent concentration on the migration and biotransformation behavior of three groundwater pollutants: ammonium (NH4 +), iron (Fe2+) and manganese (Mn2+). Columns packed with aquifer media collected from a river bank filtration (RBF) site in Harbin City, NE China were introduced synthetic groundwater (SGW) or real groundwater (RGW) were at two different constant flow rates and initial contaminant concentrations to determine the impact of system conditions on the fate of the target pollutants biotransformation. The results showed that the biotransformation rate of Fe2+ Mn2+, and NH4 + decreased by 8%, 39% and 15% under high flow rate (50 L d-1) compared to low flow rate (25 L d-1), which was consistent with the residence-time effect. While the biotransformation rate of Fe2+ Mn2+, and NH4 + decreased by 7%, 14% and 9% under high influent concentration compared to original groundwater. The 16S rRNA analysis of the aquifer media at different depths after experiments completion demonstrated that the relative abundance of major functional microbes iron oxidizing bacteria (IOB) and manganese oxidizing bacteria (MnOB) under higher flow rate and higher influent concentration decreased 13%, 14% and 25%, 24%, respectively, whereas the ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) exhibited minimal change, compared to the lower flow rate. Above all results indicated that both high flow rate and high concentration inhibit the biotransformation of NH4 +, Fe2+ and Mn2+. The biotransformation of Fe2+ and Mn2+ occurs primarily in the 0-40 cm and 20-60 cm depth intervals, respectively, whereas the NH4 + biotransformation appears to occur relatively uniformly throughout the whole 110cm column. The biotransformation kinetics of NH4 + in RGW and SGW, Mn2+ in RGW at different depths accords with the first order kinetics model, while Fe2+ in RGW and SGW, Mn2+ in SGW presented more complicated biotransformation process. The results should improve understanding of the transport and fate of common groundwater pollutants in riverbank filtration and other groundwater recharge environments.

Pathways regulating nitrogen removal in constructed ditch wetlands: effects of different inflow ratios and artificial aeration.

Constructed ditch wetland (CDW) is a combination of idle ditch and constructed wetland, which is typically used in rural areas to remove pollutants from domestic wastewater. However, its low total nitrogen (TN) removal remains a pressing issue. To enhance total nitrogen removal, an approach of supplying water at two locations in the CDW at different influent flow ratios, combined with artificial aeration, was proposed to adjust carbon and oxygen distribution in the system. The highest average TN removal was achieved at low influent concentration (CDW4; influent flow ratio 1:2). The removal of TN in winter and spring were 58.93 and 83.26%, respectively. The distribution of carbon sources in the back zone enhanced denitrification. Of the high influent concentration treatments, CDW2 (2:1) achieved 16.97% more TN removal on average compared with CDW1 (3:0), after extra artificial aeration was applied in the front zone. However, nitrification was a limiting step in the system, which became the primary problem preventing pollutant purification. Moreover, nitrifying bacteria abundance was negatively correlated to the influent flow ratio and autotrophic denitrifying bacterial abundance was positively correlated to the influent flow ratios. Graphical abstract.

Simulation and optimization of layered bioretention facilities by HYDRUS-1D model and response surface methodology

The parameters of bioretention facilities have important effects on their operations. However, previous studies have either examined the regulation effects of these parameters or conducted scenario simulations, whereas very few studies have attempted to optimize the key parameters of low impact development (LID) facilities. To fill this gap, the key parameters of bioretention facilities were optimized in this work to improve the operation of these facilities. Simulated rainfall experiments were also conducted in a school test field to monitor the regulation effect of bioretention tanks on water quantity and quality under different scenarios. The operation of bioretention facilities in other scenarios (compared with the experimental scenario) was simulated by using the HYDRAUS-1D model on the basis of the experimental data. The key parameters that greatly influence the regulation effect of bioretention facilities were defined by using the Morris classification screening method and were optimized by using the simulation results and the response surface methodology. Results show that the water reduction rate ranges from 80% to 85% and that the thickness of the medium layer ranges from 25 cm to 120 cm under a high influent concentration. Meanwhile, the bioretention tanks with an artificial filler of fly ash + sand or planting soil can treat more scenario rainfall (recurrence period of 2 years + confluence ratio of 10:1–20:1, recurrence period of 5 years + confluence ratio of 10:1–15:1, and recurrence period of 10 years + confluence ratio of 10:1), whereas the bioretention tank with artificial filler of blast furnace slag can deal with less scenario rainfall (recurrence period of 2 years/5 years/10 years + confluence ratio of 10:1 and recurrence period of 2 years + confluence ratio of 15:1).

Fate of antibiotics, antibiotic-resistant bacteria, and cell-free antibiotic-resistant genes in full-scale membrane bioreactor wastewater treatment plants

The removal of antibiotics, antibiotic-resistant bacteria (ARB), and cell-free antibiotic-resistant genes (ARGs) and the microbial community of ARB were investigated in detail to understand their fate and provide valuable information on the feasibility of full-scale membrane bioreactor (MBR). The potential risks of cell-free ARGs to the receiving environment were discovered. High influent antibiotic concentration could inhibit the microbial activity of MBR sludge, whereas good antibiotic removal could be maintained because of relatively long solid retention time and high biomass retention. Approximately 61.8%–77.5% of the total antibiotics were degraded, and 22.5%–38.2% of the total antibiotics were adsorbed by MBR sludge on average. The individual antibiotic removal presented intense discrepancy because of the chemical construction and distribution coefficient of antibiotics. Aeromonas exhibited specific antibiotic resistance to ampicillin and erythromycin, Escherichia became the predominant genera in kanamycin-ARB and tetracycline-ARB, and Klebsiella and Bacteroides were the particular genera that exhibited distinct antibiotic resistance to ciprofloxacin. A significant correlation was found between cell-free ARG abundance and ARB content, and relatively high effluent cell-free ARG abundance facilitated the proliferation and transmission of ARB. The impacts of the receiving environment to eliminate the ecological risks and severe threats to human health should be investigated because of the low decay ratio and long-term persistence of cell-free ARGs.

Preliminary evaluation of constructed wetlands for nutrients removal from olive mill wastewater (OMW) after passing through a sand filter

The current study was carried out to test the removal of nitrogen and phosphorus from olive mill wastewater (OMW) by combination of sand filter and a mixture of aquatic plants (Phragmites australis, Typha latifolia and Arundo donax). The experimental pilot was composed of two serial systems: sand filter followed by vertical flow constructed wetlands (VF-CWs). The sand filter was filled with 50 cm of sand and 10 cm of gravel in the top and the bottom of the filter. The second step of the treatment consists of a tank (1 × 1 × 1 m3) filled with 20 cm of gravel and 60 cm of soil, planted with a mixture of aquatic plants at a density of 8 young plants/m2 for each plant and irrigated twice in the week by 20L of preliminary filtered OMW. During all experiment, OMW was diluted with domestic wastewater at a ration 1/1 (v/v).Mean diluted OMW concentrations were 2.05 g/L, 1.52 g/L, 1.94 mg/L, 1.70 mg/L, 0.22 g/L, 0.30 g/L, 26.78 g/L and 20.47 g/L for TKN-N, NH4+-N, NO2−-N, NO3−N, ortho-phosphate (OP), Total phosphorus (PT) total COD and dissolved COD respectively. Despite the rather high influent concentrations, the performance of the sand filter was very effective since it achieved average removals of about 60.4%, 74.4%, 80.61%, 97.85%, 96.45%, 79.75% and 76.75% for NTK, NH4+, NO3−, PO43−, PT total COD and dissolved COD respectively. The presence of aquatic plants was more efficient in removing of nutrients and organic load. The average elimination of experimental system (sand filter + VF-CWs) in terms of flow was 62.48% for NTK, 90.43% for NH4+, 77.25% for NO3−, 98.51% for PO43−, 97.53% for PT and 99.05% for total COD.In general, this study shows potential application of the experimental system to achieve nutrient and COD removal from OMW. Although the percent removal of organic matter and nutrients from OMW was very high, final OMW concentrations remained high, thus preventing their use for irrigation or immediate disposal into the environment.