Higher Bone Mineral Content Research Articles

Potential Effects of Lifelong Team Handball and Football Training and Nutritional Habits on Bone Health and Body Composition in Elderly Women.

The aim of this study was to evaluate the effects of lifelong team handball/football training on regional bone health and body composition in elderly women. Seventeen elderly women team handball/football players (65.9 ± 5.7 years) and twenty-one untrained age-matched women (controls) (67.7 ± 5.1 years) participated. Whole-body and regional dual-energy X-ray absorptiometry scans of arms, legs, and lower spine (L1-L4) were performed. We observed 8% and 9% higher bone mineral density (BMD) and bone mineral content (BMC), respectively, at the whole-body level and in the legs and 11.5% higher BMC in the legs in team handball/football players compared to untrained age-matched controls (p < 0.05). Higher total and leg lean body mass (p < 0.05), along with lower total body fat percentage (p < 0.05) and higher T- and Z-scores, markers of fragility risk fracture (0.294 ± 1.461 vs. -0.538 ± 1.031; 1.447 ± 1.278 vs. 0.724 ± 0.823, respectively), were also found in team handball/football players compared to controls (p < 0.05). No significant differences in nutritional habits were observed between groups. Our study suggest that the beneficial effects of lifetime handball/football practice on bone preservation in elderly women occur independently from nutritional intake, which emphasize the potential role of team sports in osteoporosis prevention. Future studies should focus on the cofounding factors and causative mechanisms mediated by team sport practice in osteoporosis prevention.

Bone Health, Body Composition and Physiological Demands in 70-85-Year-Old Lifelong Male Football Players.

The effects of lifelong football training on bone health, body composition and physiological demands were evaluated. A total of 20 veteran football players (VPG; 73.4 ± 3.7 years) and 18 untrained age-matched men (CG; 75.6 ± 4.2 years) were enrolled. Whole-body and regional dual-energy X-ray absorptiometry scans of arms, legs, proximal femur and lower spine (L1-L4) were recorded in all participants. We observerd higher bone mineral density (BMD) in the whole-body, arms and femoral regions and higher bone mineral content (BMC) in the legs and lower spine compared to the CG (p < 0.05), also higher total lean body mass (p < 0.05) and lower total body fat percentage (p < 0.05), were found. No differences in food habits were evidenced between the VPG and the CG, as evaluated using 3-day food records. Resting heart rate (RHR), blood pressure (BP) and activity profile during a football match were recorded using a global positioning system only in the VPG. The mean heart rate (HR)of theoretical maximal HR (ThHRmax), and peak of ThHRmax were 83.9 ± 8.6% and 98.6 ± 10.2%, respectively; the mean of total distance covered was 3666 ± 721 m, and the means of accelerations and decelerations were 419 ± 61 and 428 ± 65, respectively. Lifelong participation in football training improves regional BMD and BMC in legs, femur and lumbar spine compared to the CG. A high number of intense actions in term of HR and accelerations and decelerations suggests an elevated energy expenditure that in turn correlates to the healthier body composition observed in the VPG compared to the CG.

Improved Predictions of Human Rib Structural Properties Using Bone Mineral Content

&lt;div&gt;Rib fractures are associated with high rates of morbidity and mortality. Improved methods to assess rib bone quality are needed to identify at-risk populations. Quantitative computed tomography (QCT) can be used to calculate volumetric bone mineral density (vBMD) and bone mineral content (BMC), which may be related to rib fracture risk. The objective of this study was to determine if vBMD and BMC from QCT predict human rib structural properties. 127 mid-level (5th–7th) ribs were obtained from adult female (&lt;i&gt;n&lt;/i&gt; = 67) and male (&lt;i&gt;n&lt;/i&gt; = 60) postmortem human subjects (PMHS). Isolated rib QCT scans were performed to calculate vBMD and BMC. Each rib was subsequently tested to failure in a dynamic simulated frontal impact and structural properties, peak force (&lt;i&gt;F&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt;), percent displacement (&lt;i&gt;δ&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt;), linear structural stiffness (&lt;i&gt;K&lt;/i&gt;), and total energy (&lt;i&gt;U&lt;/i&gt;&lt;sub&gt;Tot&lt;/sub&gt;) were calculated. vBMD demonstrated no significant differences between sexes (&lt;i&gt;p&lt;/i&gt; &amp;gt; 0.05); however, males had a higher BMC than females (&lt;i&gt;p&lt;/i&gt; &amp;lt; 0.001). Further, sex-specific differences were observed in all rib structural properties except for &lt;i&gt;δ&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt; (&lt;i&gt;p&lt;/i&gt; &amp;gt; 0.05). Age had a significant relationship with both vBMD and BMC (&lt;i&gt;p&lt;/i&gt; &amp;lt; 0.001) but only in females when separated by sex (&lt;i&gt;p&lt;/i&gt; &amp;lt; 0.001). vBMD predicted &lt;i&gt;F&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt;, &lt;i&gt;δ&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt;, &lt;i&gt;K&lt;/i&gt;, and &lt;i&gt;U&lt;/i&gt;&lt;sub&gt;Tot&lt;/sub&gt; (&lt;i&gt;R&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; = 9.2%–30.9%, &lt;i&gt;p&lt;/i&gt; &amp;lt; 0.05) but was not able to predict &lt;i&gt;δ&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt; in males. Similarly, BMC also predicted all rib structural properties, except for &lt;i&gt;δ&lt;/i&gt;&lt;sub&gt;Peak&lt;/sub&gt; in males, but explained more meaningful amounts of variation (&lt;i&gt;R&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; = 22.2%–67.7%, &lt;i&gt;p&lt;/i&gt; &amp;lt; 0.001). When predicting rib structural properties, BMC captures sex-specific variations in bone size that are obfuscated by vBMD and contribute to the biomechanical response of the rib during mechanical loading. Incorporating BMC into assessments of injury risk may therefore provide additional insight into the multifaceted nature of rib bone quality and differential fracture resistance.&lt;/div&gt;

Associations of Dietary Anthocyanidins Intake with Bone Health in Children: A Cross-Sectional Study.

Bone health and body composition share several common mechanisms like oxidative stress and inflammation. Anthocyanins have antioxidant and anti-inflammatory properties. We have reported that anthocyanins are associated with better body composition in children, but the associations with bone health have not been elucidated. We aimed to explore the association of anthocyanins with bone mineral content (BMC) and bone mineral density (BMD) at multiple sites in children. In this cross-sectional study, 452 Chinese children aged 6-9years were recruited. A validated 79-item food frequency questionnaire was used to collect dietary information. BMC and BMD at multiple sites (whole body; whole body excluding head, WBEH; limbs; arms; legs) were measured by dual-energy X-ray. Higher dietary intake of total anthocyanidins (per one standard deviation increase) was associated with a 1.28-13.6g (1.31-1.60%, compared to median) higher BMC at all sites and a 3.61-6.96mg (0.65-0.90%) higher BMD at the whole body, WBEH, and arm sites after controlling for a number of possible covariates. The results were similar and more pronounced for cyanidin, but not for delphinidin and peonidin. Higher dietary intake of cyanidin (per one standard deviation increase) was associated with a 1.33-15.4g (1.48-1.68%) higher BMC at all sites and a 4.15-7.77mg (0.66-1.00%) higher BMD at all sites except the legs. No statistically significant associations with BMC or BMD were found for dietary intake of delphinidin and peonidin. Higher dietary intake of total anthocyanidins and cyanidins were associated with higher BMC and BMD in Chinese children.

The effects of myostatin mutation on the tibia bone quality in female Japanese quail before and after sexual maturation

In the modern layer industry, improvement of bone quality is one of the prior tasks to solve from economic and welfare standpoints. In addition to nutritional and environmental factors, genetic factors have been considered major factors regulating bone quality in layers but are yet to be fully investigated due to limitations on available animal models. Initially, the myostatin (MSTN) gene was genetically edited in quail to investigate the effect of MSTN mutation on economic traits in meat producing poultry species. In the current study, the function of the MSTN gene on regulation of bone quality in layers was investigated using MSTN mutant female quail as an animal model. Tibia bones were collected from wild-type (WT) and MSTN mutant female quail at 5 wk old and 4 mo old, representing prelaying and actively laying stages, respectively. Left tibia bones were analyzed by microcomputed tomography scanning to evaluate the architectural characteristics, while bone breaking strength (BBS) was measured using right tibia bones. At 5 wk of age, MSTN mutant female quail showed higher BBS and values on parameters related to bone quality such as bone mineral contents (BMC), bone mineral density (BMD), bone volume (BV), and/or trabecular bone thickness in whole diaphysis, whole metaphysis, and metaphyseal trabecular bone, compared to WT female quail. Although BBS and BMD became similar between the 2 groups at 4 mo of age, higher TV and TS in whole metaphysis and higher BMC and TV in whole diaphysis of MSTN mutant group compared to those of WT group suggested that the improved tibia bone quality by MSTN mutation before sexual maturation lasted to a certain degree even after sexual maturation. The use of the MSTN mutant female model provided new insights into genetic regulation on female quail bone quality depending on physiological changes.

Polymorphisms in Genes Encoding VDR, CALCR and Antioxidant Enzymes as Predictors of Bone Tissue Condition in Young, Healthy Men.

The aim of the study was to assess significant predictors of bone mineral content (BMC) and bone mineral density (BMD) in a group of young, healthy men at the time of reaching peak bone mass. Regression analyses showed that age, BMI and practicing combat sports and team sports at a competitive level (trained vs. untrained group; TR vs. CON, respectively) were positive predictors of BMD/BMC values at various skeletal sites. In addition, genetic polymorphisms were among the predictors. In the whole population studied, at almost all measured skeletal sites, the SOD2 AG genotype proved to be a negative predictor of BMC, while the VDR FokI GG genotype was a negative predictor of BMD. In contrast, the CALCR AG genotype was a positive predictor of arm BMD. ANOVA analyses showed that, regarding SOD2 polymorphism, the TR group was responsible for the significant intergenotypic differences in BMC that were observed in the whole study population (i.e., lower BMC values of leg, trunk and whole body were observed in AG TR compared to AA TR). On the other hand, higher BMC at L1-L4 was observed in the SOD2 GG genotype of the TR group compared to in the same genotype of the CON group. For the FokI polymorphism, BMD at L1-L4 was higher in AG TR than in AG CON. In turn, the CALCR AA genotype in the TR group had higher arm BMD compared to the same genotype in the CON group. In conclusion, SOD2, VDR FokI and CALCR polymorphisms seem to affect the association of BMC/BMD values with training status. In general, at least within the VDR FokI and CALCR polymorphisms, less favorable genotypes in terms of BMD (i.e., FokI AG and CALCR AA) appear to be associated with a greater BMD response to sports training. This suggests that, in healthy men during the period of bone mass formation, sports training (combat and team sports) may attenuate the negative impact of genetic factors on bone tissue condition, possibly reducing the risk of osteoporosis in later age.

The relationship between different metabolic phenotypes and bone indices in Chinese children and adolescents aged 12-18 years old.

Data regarding different metabolic phenotypes and bone markers including bone mineral content (BMC) and osteocalcin (OCN) among children and adolescents are very limited. Hence, the purpose of this investigation was to explore the relationship between different metabolic phenotypes and BMC or OCN among Chinese children and adolescents. This cross-sectional study included 1,328 children and adolescents aged between 12 and 18 years who were selected from four schools in Yinchuan city from 2018 to 2020 by stratified cluster random sampling. Subjects were divided into four groups according to BMI and metabolic status, as follows: metabolically healthy obesity (MHO), metabolically unhealthy obesity (MUO), metabolically unhealthy normal weight (MUNW), and metabolically healthy normal weight (MHNW). The MHNW, MUNW, MHO, and MUO phenotypes in boys were 48.4%, 30.5%, 6.7%, and 14.4%, respectively, and were 47.8%, 33.6%, 6.6%, and 12.1% in girls, respectively. The MHO and MUO phenotypes had higher BMC than the MHNW or MUNW phenotype (all p < 0.05), and the MUO phenotype with BMC was significantly higher than MHO group in boys (p < 0.05). We discovered a significant positive correlation between BMC and the MHO (OR = 8.82, 95% CI = 2.04-38.16), MUO phenotypes (OR = 13.53, 95% CI = 4.10-44.70), while no association was found between OCN and metabolic phenotypes in neither boys nor girls. Overweight/obese children and adolescents had higher BMC, and there existed sex differences in the effect of metabolic status on BMC among them. OCN was not supposed to be an index of bone health in this study.

Associations between three diet quality indices, genetic risk and body composition: A prospective cohort study

Diet and genetic predisposition to adiposity are independent predictors of body composition, yet few cohort studies have examined the association between overall diet quality indices, genetic risk and body composition. This study examined the prospective association of three diet quality indices and a polygenic risk score (PRS) with trunk fat mass, total fat mass, lean mass and bone mineral content. Adults from UK Biobank cohort were included. Dietary intake was assessed using the Oxford WebQ and three diet quality indices calculated: Recommended Food Score (RFS); Mediterranean Diet Score (MDS); Healthy Diet Indicator (HDI). Bioimpedance data were available for trunk fat, total fat and lean mass (kg). Trunk fat mass (kg), total fat mass (kg) and lean mass (kg) were assessed using bioelectrical impedance (BIA) in 17,478 adults. Bone mineral content (g) was available from dual energy x-ray absorptiometry (DXA) scans in 11,887 participants. Linear regression analyses, adjusted for demographic and lifestyle confounders, were used to estimate prospective associations between each diet quality index and body composition outcomes. A PRS created from 97 adiposity-related single nucleotide polymorphisms was used to examine interaction effects. A total of 17,478 adults (M=55.9, SD 7.5 years) were followed up for up to 10 years. RFS, HDI and MDS were inversely associated with trunk fat (RFS: B-0.29; 95% CI:-0.33,-0.25; HDI:-0.23;-0.27,-0.19; MDS:-0.22;-0.26,-0.18), total fat (RFS: B-0.49; 95% CI:-0.56,-0.42; HDI:-0.38;-0.45,-0.32; MDS:-0.38;-0.44,-0.32) and lean (RFS: B-0.10; 95% CI:-0.14,-0.06; HDI:-0.07;-0.11,-0.03; MDS:-0.07;-0.11,-0.04) mass. Diet quality was positively associated with bone mineral content (RFS: B 8.23; 95% CI: 2.14, 14.3; HDI: 6.77; 1.00, 12.5). There was evidence of non-linear associations between diet quality (RFS and HDI only) and trunk fat (p<0.01) and total fat mass (p<0.05). There was limited evidence PRS was associated with body composition, with interaction effects of PRS and HDI (p-interaction=0.039) and MDS (p-interaction=0.031) on total fat mass. Higher diet quality was associated with lower trunk fat, total fat and lean mass, regardless of the diet quality index examined (RFS, HDI or MDS), while higher diet quality (RFS and HDI only) was associated with higher bone mineral content. The benefit of higher diet quality on reducing total fat mass was most evident in individuals with higher generic risk of adiposity. These findings underscore the importance of a high-quality diet for maintaining optimal body composition, particularly in individuals with genetic pre-disposition to adiposity.

Benefits of Two 24-Week Interactive Cognitive-Motor Programs on Body Composition, Lower-Body Strength, and Processing Speed in Community Dwellings at Risk of Falling: A Randomized Controlled Trial.

This 24-week randomized controlled trial study evaluated the effects of two interactive cognitive–motor programs on body composition, lower-body strength, and processing speed in community dwellings at risk of falling. Forty-eight participants (75.0 ± 5.4 years) were allocated into EG1 (psychomotor intervention program), EG2 (combined program (psychomotor intervention + whole-body vibration)), and a control group. EG programs induced significant improvements in bone mass, lower-body strength, and processing speed (p < 0.05), with similar treatment effects on lower-body strength and processing speed and higher bone mineral content and density within EG2. The fall rate decreased in EG1 (44.2%) and EG2 (63%) (p < 0.05). After the 12-week no-intervention follow-up, improvements in lower-body strength were reversed in both EGs, but those in processing speed were maintained, mainly in EG2 (p < 0.05). In conclusion, both programs were accepted and well tolerated. The combined program led to additional benefits in bone mass. Both programs positively impacted physical and cognitive risk factors for falls and injuries. They induced similar improvements in lower-body strength and processing speed, decreasing the fall rate. These findings suggest that both programs are successful for fall and injury prevention in the studied population.

RELATIONSHIP BETWEEN BODY FAT DISTRIBUTION AND BONE MINERAL CONTENT IN CHINESE ADOLESCENTS

Objective: The aim of this study is to analyze the relationship between body fat distribution and bone mineral content (BMC), and gender difference among Chinese adolescents. Design and method: A total of 1575 adolescents aged 12–18 were randomly selected in China by stratified cluster sampling. BMC and body fat distribution indexes, included fat mass (FM), appendicular fat mass (AFM), and trunk fat mass (TFM), were measured by Bioelectrical Impedance Analysis (BIA). Results: Except for limb to total fat ratio (LTFR), girls’ FM, AFM, TFM, trunk to total fat ratio (TrTFR) and trunk to limb fat ratio (TrLFR) were higher than boys (P values &lt; 0.05). There was a statistically significant negative correlation between LTFR and BMC (boys: r = -0.630, girls: r = -0.351), while other indexes were positively correlated with BMC (all P values &lt; 0.05). Regression analysis revealed that the higher BMC in the fourth tertile of TrTFR was 34.23 (17.49, 67.00) times versus that of the control group in boys. Similarly, the higher BMC in the fourth tertile of TrLFR was 45.09 (5.84,348.38) times than that in the control group in girls (P value &lt; 0.05). Conclusions: There are gender differences in the distribution of body fat in Chinese adolescents. And during this period, higher central fat mass has a positive effect on the accumulation of BMC.

Osteoprotective Effects in Postmenopausal Osteoporosis Rat Model: Oral Tocotrienol vs. Intraosseous Injection of Tocotrienol-Poly Lactic-Co-Glycolic Acid Combination.

Osteoporosis, the most common bone disease, is associated with compromised bone strength and increased risk of fracture. Previous studies have shown that oxidative stress contributes to the progression of osteoporosis. Specifically, for postmenopausal osteoporosis, the reduction in estrogen levels leads to increased oxidative stress in bone remodeling. Tocotrienol, a member of vitamin E that exhibits antioxidant activities, has shown potential as an agent for the treatment of osteoporosis. Most studies on the osteoprotective effects of tocotrienols had used the oral form of tocotrienols, despite their low bioavailability due the lack of transfer proteins and high metabolism in the liver. Several bone studies have utilized tocotrienol combined with a nanocarrier to produce a controlled release of tocotrienol particles into the system. However, the potential of delivering tocotrienol–nanocarrier combination through the intraosseous route has never been explored. In this study, tocotrienol was combined with a nanocarrier, poly lactic-co-glycolic acid (PLGA), and injected intraosseously into the bones of ovariectomized rats to produce targeted and controlled delivery of tocotrienol into the bone microenvironment. This new form of tocotrienol delivery was compared with the conventional oral delivery in terms of their effects on bone parameters. Forty Sprague–Dawley rats were divided into five groups. The first group was sham operated, while other groups were ovariectomized (OVX). Following 2 months, the right tibiae of all the rats were drilled at the metaphysis region to provide access for intraosseous injection. The estrogen group (OVX + ESTO) and tocotrienol group (OVX + TTO) were given daily oral gavages of Premarin (64.5 mg/kg) and annatto-tocotrienol (60 mg/kg), respectively. The locally administered tocotrienol group (OVX + TTL) was given a single intraosseous injection of tocotrienol–PLGA combination. After 8 weeks of treatment, both OVX + TTO and OVX + TTL groups have significantly lower bone markers and higher bone mineral content than the OVX group. In terms of bone microarchitecture, both groups demonstrated significantly higher trabecular separation and connectivity density than the OVX group (p < 0.05). Both groups also showed improvement in bone strength by the significantly higher stress, strain, stiffness, and Young’s modulus parameters. In conclusion, daily oral tocotrienol and one-time intraosseous injection of tocotrienol–PLGA combination were equally effective in offering protection against ovariectomy-induced bone changes.

Clinical bone health among adults with cerebral palsy: moving beyond assessing bone mineral density alone.

To understand associations among bone mineral density (BMD), bone mineral content (BMC), and bone area, and their association with fractures in adults with cerebral palsy (CP). This retrospective cohort study included 78 adults with CP with a hip dual energy X-ray absorptiometry (DXA) from 1st December 2012 to 3rd May 2021 performed at the University of Michigan. Data-driven logistic regression techniques identified which, if any, DXA-derived bone traits (e.g. age/sex/ethnicity-based z-scores) were associated with fracture risk by sex and severity of CP. BMC-area associations were examined to study the structural mechanisms of fragility. Femoral neck area was associated with lower age-adjusted odds ratios (ORs) of fracture history (OR 0.72; 95% confidence interval [CI] 0.49-1.06; p=0.098), while higher BMD was associated with higher odds of incident fracture (OR 3.08; 95% CI 1.14-8.33; p=0.027). Females with fracture had lower area than females without fracture but similar BMC, whereas males with fracture had larger area and higher BMC than males without fracture. The paradoxical BMD-fracture association may be due to artificially elevated BMD from BMC-area associations that differed between females and males (sex interaction, p˂0.05): males had higher BMC at lower area values and lower BMC at higher area values compared to females. BMD alone may not be adequate to evaluate bone strength for adults with CP. Further research into associations (or integration) between BMC and area is needed.

Effects of backhand stroke styles on bone mineral content and density in postmenopausal recreational tennis players: a cross-sectional pilot investigation

BackgroundOne-handed backhand (OB) and two-handed backhand (TB) styles are commonly used in tennis, but only TB generates loadings on the non-dominant arm and a greater extension torque on the rear leg, leading to a greater axial torque involving rotation of the hip and trunk. The current study investigated whether those effects can further affect bone area (BA), bone mineral content (BMC) and density (BMD) in postmenopausal recreational tennis players.MethodsBA, BMC and BMD of the lumbar spine, hip and distal radius were assessed using dual-energy X-ray absorptiometry in TB, OB, and swimmers’ group as a control (SG) (all participants self-reported for at least 5 years of exercise history, n = 14 per group). Muscular strength was assessed with a hand dynamometer. Among these three groups, the BA, BMC and BMD of distal radius and muscle strength were assessed using one-way ANOVA, and those of the lumbar region and the hip joint were tested by one-way ANCOVA.ResultsTB showed higher BMC and BMD for both lumbar spine and femoral neck than SG (all, p < 0.05). Both OB and TB showed greater BMD inter-trochanter than SG (both, p < 0.05). OB demonstrated greater inter-arm differences in the distal radius, which involved 1/3 distal for BMC and mid-distal radius for BMD compared to the TB and SG (all, p < 0.05). In addition, greater inter-arm asymmetry of grip strength was found in OB compared to TB and SG (both, p < 0.05).ConclusionFor postmenopausal women, performing two-handed backhand strokes, leads to higher BMC and BMD in the non-dominant arm, the lumbar region, and hips, indicating potential benefit to maintain bone health and strength. Whether this result leads to reducing the risk of osteoporosis needs to be investigated in further research.

Food Creatine and DXA-Derived Body Composition in Boys and Girls Aged 8 to 19 Years.

Several small-scale trials indicate a positive correlation between dietary creatine intake and fat-free mass in the pediatric population; whether this connection occurs at the population-wide level remains currently unknown. The main purpose of this cross-sectional study was to calculate the amount of creatine consumed through a regular diet among U.S. boys and girls aged 8 to 19 years, and investigate the link between creatine consumption and dual-energy X-ray absorptiometry (DXA)-derived body composition indices in this population. Data were obtained from the National Health and Nutrition Examination Survey 2017-2018 round, with dietary information and whole-body DXA body composition measures extracted for respondents aged 8 to 19 years (1273 participants, 649 boys and 624 girls). Individual values for total grams of creatine consumed per day for each participant were computed using the average amount of creatine (3.88 g/kg) across all creatine-containing foods. The primary exposure was the mean daily intake of creatine; the primary and secondary outcomes comprised lean mass excluding bone mineral content (BMC), and bone mineral density, BMC, lean mass including BMC, fat mass, and percent body fat, respectively. The average intake of creatine across the sample was 0.65 ± 0.72 g/day (95% CI, from 0.61 to 0.69). Creatine positively correlated with lean mass (excluding BMC) and BMC across the whole sample (r = .18 and .20, respectively; P < .001); a significant negative correlation was found between creatine intake and percent body fat (r = −.09; P = .001). The higher intake of creatine was associated with higher lean mass in girls and higher BMC in boys, while taking more creatine corresponded to less body fat for both genders (P < .05). Our findings indicate a significant correlation between dietary creatine and DXA-derived body composition biomarkers in a nationally representative cohort of U.S. youth. These results justify further research of creatine’s role in modifying body morphology in the pediatric population, taking into account the age and sex specific traits.

Effect of soluble corn fiber supplementation for 1 year on bone metabolism in children, the MetA-bone trial: Rationale and design

Calcium intake is critical for adequate bone mineralization in adolescence, but it is usually inadequate in US adolescents. A strategy to maximize bone mineralization is to increase calcium absorption, which could be achieved by soluble corn fiber (SCF). There are no studies determining the long-term effects of SCF on bone mass in children. ObjectivesTo determine the effect of one-year SCF supplementation compared to placebo on bone mass and bone biomarkers in children with low habitual calcium intake. We hypothesize that SCF supplementation will result in a higher bone mineral content and higher levels of bone formation and lower bone resorption biomarkers. Methods240 healthy children (10–13 years), with usual low calcium intake, will be randomized to four experimental groups for 1 year: (1) SCF (12 g/d); (2) SCF (12 g/d) + 600 mg/d of calcium; (3) Placebo (maltodextrin); and (4) Placebo +600 mg/d of calcium. The supplements have been pre-mixed with a flavored powder beverage and participants will only need to dilute it in water and drink this twice per day. Bone will be measured using dual energy x-ray absorptiometry (DXA) at baseline, 6 and 12 months. Serum bone biomarkers will be measured at baseline and at 12 months. ConclusionsIf supplementing diets with SCF lead to higher bone mass during adolescence, this could help achieve the genetic potential for PBM and to start adult life with stronger bones. If successful, SCF can be incorporated into diets for promoting bone health in adolescents.

Effect of Soluble Corn Fiber Supplementation for 1 Year on Bone Metabolism in Children: MetA-Bone Trial Study Protocol-Rationale and Design

Abstract Objectives The adolescent period is crucial for optimizing future bone health because during these years bone accumulates rapidly accounting for up to half of adult peak bone mass (PBM). Calcium intake during this stage is critical for adequate bone mineralization but this essential nutrient, the major constituent of hydroxyapatite, is usually inadequate in the diets of US adolescents. A strategy to maximize bone mineralization is to increase calcium absorption. This could be achieved by soluble corn fiber (SCF) supplementation, which fermentation through bacteria in the lower intestine produces short-chain fatty acids and increase calcium absorption. However, there are no studies determining the long-term effects of SCF on bone mass in children. The main aim of the MetA-Bone Trial is to determine the effect of one-year SCF supplementation compared to placebo on bone mass in children with low habitual calcium intake. We hypothesize that SCF supplementation will result in a higher Bone Mineral Content. We will also determine the effects of SCF supplementation on bone biomarkers; we hypothesize that supplementation with SCF will result in higher levels of bone formation and lower bone resorption biomarkers. Methods A total of 240 healthy children (10–13 years), with usual low dietary calcium, will be randomized to four experimental groups for 1 year: (1) SCF (12 g/d); (2) SCF (12 g/d) + 600 mg/d of calcium; (3) Placebo (maltodextrin); and (4) Placebo + 600 mg/d of calcium. The supplements will be mixed with a flavored powder beverage to dilute in water and participants will be instructed to drink this twice per day. Bone mass will be measured using dual energy x-ray absorptiometry (DXA) at baseline, 6 and 12 months. Serum bone biomarkers will be measured at baseline and at 12 months. Results NA Conclusions If supplementing diets with SCF lead to higher bone mass during adolescence, this could help achieve the genetic potential for PBM and to start adult life with stronger bones. Incorporating SCF into diets may be a cost-effective intervention for bone health than increasing dairy products consumption, particularly in adolescents. Funding Sources Eunice Kennedy Shriver National Institute of Child Health &amp; Human Development of the National Institutes of Health.

“Effect of very dense artifacts on Hologic and general electric bone densitometry results”

Very dense objects, such as lead bullets are described as "black hole" artifacts on Hologic densitometers. Whether similar results occur on GE scanners is not reported. We hypothesized that dense artifacts confound both brands of densitometers. Three lead bullets of varying size were placed overlying or adjacent to L3 on anthropomorphic and encapsulated aluminum spine phantoms. Three scans were acquired with and without projectiles on a Hologic Discovery W, GE iDXA, and Prodigy densitometer. Lead bullets are measured as having high bone mineral content (BMC); they appear black in dual-energy mode on Hologic scanners and are colored blue on GE scanners. On Hologic scanners, BMC of a dense artifact over bone is excluded, but the bone area is not altered. Consequently, bone mineral density (BMD) of the affected vertebra, and of L1-4, is decreased. For example, a .45 caliber bullet over L3 decreased BMD (p < 0.05) by 48.3% and L1-4 by 9.1%. GE scanners excluded associated BMC and area covered by the artifact, thereby minimizing impact on BMD. Dense artifacts over soft tissue on a phantom do not substantially affect BMD on either manufacturer's densitometer when scanned. Densitometer manufacturers handle very dense artifacts differently. GE software removes artifact BMC and area with resultant minimal impact on BMD, Hologic removes only BMC, not area, thereby decreasing BMD. Regardless of this difference, when dense artifacts overlay the spine, it is best to exclude the affected vertebral body. Finally, the BMD stability observed with artifacts over soft tissue may not be replicated in humans.

Effects of ginsenoside Rg3 on bone loss, bone mineral density and osteoclast number in glucocorticoid-induced osteoporosis rats, and the likely michanism of action

Purpose: To investigate the effect of ginsenoside Rg3 on bone loss, bone mineral density (BMD) and osteoclast number in glucocorticoid-induced osteoporosis (GIOP) rats, and the mechanism of action involved.Methods: Sixty female Wistar rats were assigned to control, model group, ginsenoside Rg3, and alendronate sodium groups, comprised of 15 rats per group. The osteoporosis rat model was established via intramuscular injection of dexamethasone. Changes in bone mineral content (BMC), BMD trabecular thickness and area, osteoblasts and osteoclasts in femurs and lumbar vertebrae were measured after 3 months of treatment.Results: There were significantly higher BMC and BMD levels in ginsenoside Rg3 group than in alendronate rats (p &lt; 0.05). The thickness and trabecular area in femur and lumbar vertebrae in the ginsenoside Rg3 group were significantly higher than those in the model group (p &lt; 0.05), but were comparable with those in the alendronate sodium group (p &gt; 0.05). There were marked increases in osteoblasts, and marked decreases in osteoclasts in the ginsenoside Rg3 group, alendronate sodium and control rats, relative to model rats (p &lt; 0.05).Conclusion: Ginsenoside Rg3 arrests bone loss, and enhances bone density, trabecular thickness and area, bone microstructure, osteoblast activity and population of osteoclasts number in glucocorticoidinduced osteoporotic rats. This provides a new research direction for the clinical treatment ofosteoporosis.&#x0D; Keywords: Ginseng soap, Rg3, Glucocorticoid, Osteoporosis, Bone loss, Bone mineral density, Osteoclast population

Alpha-Lactalbumin Enriched Whey Protein Concentrate to Improve Gut, Immunity and Brain Development in Preterm Pigs.

Human milk is rich in nutritional factors, such as alpha-lactalbumin (α-Lac), and important for neonatal development, but nutrient supplementation may be required for optimal growth. Using a pig model, we hypothesized that α-Lac-enriched whey protein concentrate (WPC) supplementation improves neonatal development. Cesarean-delivered preterm pigs were fed either dilute bovine milk (REF) or REF milk supplemented with WPC with normal (STANDARD-ALPHA) or high (HIGH-ALPHA) α-Lac. Clinical, gut, immune and cognitive endpoints (open field, T-maze) were assessed and tissues collected at Day 19. The growth of STANDARD-ALPHA and HIGH-ALPHA were higher than REF (31 vs. 19 g/kg/d). Most organ weights, gut, immunity and brain variables were similar between WPC groups. HIGH-ALPHA had a higher bone mineral content, colon microbial diversity and an abundance of specific bacteria and microbial metabolites, and tended to show a faster food transit time (p = 0.07). Relative to REF, WPC pigs showed higher relative organ weights, blood amino acids, blood neutrophil function, and microbial metabolites, but lower brush-border enzyme activities and plasma cortisol. Cognition outcomes did not differ among the groups. In conclusion, WPC supplementation of milk improved some growth, gut and immunity parameters in preterm pigs. However, increasing the α-Lac content beyond human milk levels had limited effects on the immature gut and developing brain.

Infant Weight and Length Growth Trajectories Modeled Using Superimposition by Translation and Rotation Are Differentially Associated with Body Composition Components at 3 and 7 Years of Age

To evaluate how infant weigh and length growth trajectories associate with body composition at 3 and 7 years because previous studies have noted that rapid infant weight gain increases risk for high body mass index (BMI) in children. There were 322 children enrolled at 3 years of age with dual x-ray absorptiometry body composition data and pediatrician growth data for 0-2 years of age who were included in analysis. Superimposition by translation and rotation modeling was used to characterize infant weight and length trajectories in terms of size, tempo and velocity measures. Associations of these measures with fat mass, lean mass, percent body fat, bone mineral content, BMI z-score, and overweight prevalence at 3 and 7 years of age were determined. Infant growth trajectories differed by sex, race, and breastfeeding status. Higher overall weight size and weight velocity from 0 to 2 years of age were associated positively with all age 3 body composition and anthropometry outcomes. However, longer length size from 0 to 2 years of age was associated independently with higher bone mineral content and lean mass, but lower percent body fat, BMI z-score, and a lower odds of overweight at 3 years of age. By 7 years of age, later than average infant weight tempo was also associated with lower fat mass, lean mass, and BMI z-score. Greater average weight size and greater weight velocity in infancy are markers for greater overall body size at 3 and 7 years of age. However, longer average lengths and later weight gain tempo between 0 and 2 years of age may help to establish a leaner body composition by 3 and 7 years of age.