High Levels Of Zn Research Articles

Trade-offs and adaptation to metalliferous soils: The role of soil microbiome in metal tolerance and uptake in Arabidopsis halleri ecotypes from a reciprocal transplant experiment

Soil contamination with trace metal elements is a worldwide issue, prompting research on plant species capable of hyperaccumulating metals to reduce soil toxicity. Previous research suggests that both plant species and their populations can affect soil microbial communities, yet little is known about how different populations of hyperaccumulator species influence these microbial communities to enhance metal-uptake and tolerance. This study evaluated the effect of soil origin, soil microbiome, and plant population on phytoextraction efficiencies of Cd and Zn among four A. halleri populations: two each from metalliferous and non-metalliferous sites. In a controlled transplant experiment, clonal replicates of A. halleri were grown in native and three non-native soils for six months. Biogeochemical analyses of plants and soils were conducted, alongside sequencing of root-associated soil bacterial/archaeal and fungal DNA. Soil treatments primarily differed in pH, total Cd, Pb, and Zn, as well as acid and alkaline phosphatase enzyme activities. A combined effect of soil origin and population was noted for arylsulfatase and β-glucosidase activities, as well as ammonium and nitrate concentrations. Both non-metallicolous and metallicolous populations accumulated high levels of Cd and Zn in metalliferous soils with the non-metallicolous population NM_PL14 outperforming the metallicolous populations in Zn hyperaccumulation. Interestingly, non-metallicolous populations grown in metalliferous soils exhibited no trade-offs in plant performance despite higher Cd and Zn accumulation. Soil origin had a stronger effect on the bacterial/archaeal and fungal community composition than plant ecotype. Partial least square regression models explained 66 % and 79 % of the variability in A. halleri Cd and Zn hyperaccumulation. There was a positive association between Zn-uptake and specific microbial taxa (e.g., Cornebacteriales, Microbacteriaceae, Propionibacteriales, Rhizobiaceae, Basidiomycota, Oidiodendron, Phallaceae) and functional activity (e.g., arylsulfatase, S oxidation) in metalliferous soils. Taken together, our findings suggest that non-metallicolous A. halleri populations may be better suited for Zn phytoextraction applications.

Metal and arsenic contamination of a terrestrial top-predator, the black kite (Milvus migrans), after the Aznalcóllar mine spill (southwestern Spain): Temporal trends and fitness consequences

Top-predators serve as sentinels of ecosystem health due to their sensitivity to environmental contamination. However, our understanding on how contaminants affect individual fitness is still scarce, especially for long-lived species. Here, we measured blood concentrations of 4 metals (Cd, Cu, Pb, and Zn) and one metalloid (As) in black kite (Milvus migrans) adults from Doñana National Park (SW Spain) following a major mine spill. Besides temporal variation (1998–2001) in metal and As concentrations, we tested how metal and As profiles changed across individuals in relation to their sex, age or breeding status, and examined whether metal and As concentrations affected individuals' fitness (breeding success and mortality). We found that, overall, blood concentrations of Pb, Cd and As increased throughout the study period in black kites, mirroring the progressive increase previously reported for their main prey. Both sex (Cu and Zn) and breeding status (Zn and Pb) affected element concentrations. Non-breeding (floater) females had higher levels of Zn than their breeding counterparts. The same pattern of higher contamination in floaters was observed for Pb, which might be related to differences in diet and foraging activity between breeders and floaters. The percentage of the individuals with Pb concentrations that exceeded toxicity thresholds (> 200 μg/L) was relatively high (15.4 %). Moreover, Pb concentrations were negatively correlated to males' breeding success. We found no clear evidence to support metal and As effects on survival or life expectancy after accounting for environmental and individual sources of variation. Our results highlight the importance of long-term studies of marked individuals in wild populations; detailed knowledge of ecological processes relevant to these populations, combined with measures of contaminant exposure at individual level, provides opportunities to enhance our understanding of its fitness effects and potential demographic consequences.

Effect of Different Zn Concentrations on Crop Growth and Root Nodulation in Yard-Long Bean (Vigna unguiculata L.)

An experiment was conducted to study the effect of Zinc (Zn) as a micronutrient on growth and nodulation in Vigna unguiculata L. The experiment was carried out as a pot experiment in the Crop Farm, Faculty of Agriculture, Palachcholai, Eastern University, Sri Lanka from August to November 2023. The experiment was laid out in Complete Randomized Design with six treatments and four replicates. The treatments are T1 (Control), T2 (50 mg ZnSO4/kg soil), T3 (100 mg ZnSO4/kg soil), T4 (150 mg ZnSO4/kg soil), T5 (200 mg ZnSO4/kg soil) & T6 (250 mg ZnSO4/kg soil). The experiment used source of Zinc in the form of Zinc Sulphate (ZnSO4,5H2O) which yard-long bean plants can grow. The experiment results showed that, T2 treatment, the soil treated with 50 mg ZnSO4/kg soil significantly increase the Plant height, Chlorophyll content, Leaf area, Fresh weight of shoot & root, Dry weight of shoot & root, Number of nodules, Effective nodule percentage, as well as Soil respiration compared to the other treatments. However, beyond the level of 200 mg ZnSO4/kg soil, reduces the growth & nodulation of Vigna unguiculata L. and this shows that the level 200 mg ZnSO4/kg soil and beyond that level were the toxic level to the Vigna unguiculata L. Accordingly, the treatment T2 (50 mg ZnSO4/kg soil) was at the micronutrient level of Zn concentration which involved in many key cellular functions and show the best positive effect on growth and nodulation in Vigna unguiculata L. The results clearly indicate that, Zn can be use as a micronutrient up to a trace level in inducing plant growth and nodulation. However, at higher levels of Zn may act as heavy metal and cause phyto-toxicity in plants.

Assessment of heavy metals distribution and environmental risks in biochar from co-pyrolysis of sewage sludge and mixed municipal waste.

The substantial heavy metal concentrations in sewage sludge (SS) from wastewater treatment pose environmental risks and limit its practical applications. To mitigate this, SS was co-pyrolyzed with mixed municipal waste (MMW), and the heavy metals (HMs) distribution in the resulting biochar and leachate was analyzed through ICP-MS. Ecological risk (Er) and geological accumulation index (Igeo) were evaluated for various blending ratio. Results showed that MMW biochar exhibited high carbon and low sulfur content, indicating potential for carbon sequestration and soil enhancement. Both biochar showed intricate pore structures, with a slight increase in surface area, and greater aromaticity observed with the addition of MMW. Pyrolysis concentrated HMs in SS biochar, but enhanced stability reduced their leachate levels. Adding MMW decrease the level of HMs in biochar and leachate, notably reducing Se leaching from 1.54% to 0.12% and slightly lowering Hg and Cu levels. Co-pyrolysis stabilized Se by combining it with organic matter. Er analysis indicated a reduction in Hg and Cd risks by 86.6 and 41.23, respectively, with cumulative ecological risk (RI) dropping from 339.7 to 175.7. Higher MMW ratios reduced Igeo values for Zn, Cd, and Cu, shifting risks from strong to moderate and improving biochar’s agricultural suitability. High Cu and Zn levels in SS, originating from zinc and copper pipes in water treatment, were mitigated by MMW blending. Hence, the optimal co-pyrolysis ratio for HMs immobilization and SS disposal is 50% MMW. These results emphasize the importance of co-pyrolysis to produce safe biochar with reduced ecological risks. However, further research is required to optimize co-pyrolysis for various sludge types at higher pyrolysis temperatures (beyond 400℃) with the application of acid leaching agents.

Assessment of microplastic and heavy metal pollution in agricultural soils of Ernakulam District, Kerala, India.

Microplastics (MPs) and heavy metal pollution pose significant environmental threat, potentially leading to agroecosystem toxicity and jeopardizing food security. Therefore, this study aims to evaluate the abundance and risk assessment of these pollutants in five different farmlands of Ernakulam district, India. Results showed that MPs content in agricultural fields near commercialized areas such as Kakkanad Nedungapuzha, Nedumbassery, and Kadamakuddy was dominant compared to Nechoor, a rural area. The average microplastic abundance was found to be 45.6 ± 26.4 items kg⁻1 dw. Polypropylene (PP) and polyethylene (PE) were the dominant polymers found in the soil samples, constituting 45% and 25% of the microplastic content, respectively. The pollution load index of MPs indicates that the sampling sites are considered to be polluted as PLI > 1 with hazard level I. The heavy metal pollution status follows the order: Cu (80.3 to 724mg/kg) > Zn (77 to 543.5mg/kg) > Cr (171.65 to 334.65mg/kg) > As (10.25 to 79.5mg/kg) > Pb (2.05 to 30.3mg/kg) > Cd (0.3 to 14.35mg/kg). Calculated pollution load index (PLI) geo-accumulation index (Igeo), ecological risk assessment values indicate that commercialized regions exhibit high levels of trace metals, namely Cu, Zn, As, Cd, and Cr, posing a significant concern for the agricultural ecosystem. Our results indicate heightened microplastics and heavy metals prevalence in farmlands adjacent to commercial zones, necessitating immediate preventive action to mitigate increasing concentrations.

The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance.

Zinc is a vital transition metal for all bacteria; however, elevated intracellular free Zn levels can result in mis-metalation of Mn-dependent enzymes. For Mn-centric bacteria such as Streptococcus pneumoniae that primarily use Mn instead of Fe as an enzyme cofactor, Zn is particularly toxic at high concentrations. Here, we report our identification and characterization of the function of the five homologous, CiaRH-regulated Ccn sRNAs in controlling S. pneumoniae virulence and metal homeostasis. We show that deletion of all five ccn genes (ccnA, ccnB, ccnC, ccnD, and ccnE) from S. pneumoniae strains D39 (serotype 2) and TIGR4 (serotype 4) causes Zn hypersensitivity and an attenuation of virulence in a murine invasive pneumonia model. We provide evidence that bioavailable Zn disproportionately increases in S. pneumoniae strains lacking the five ccn genes. Consistent with a response to Zn intoxication or relatively high intracellular free Zn levels, expression of genes encoding the CzcD Zn exporter and the Mn-independent ribonucleotide reductase, NrdD-NrdG, were increased in the ΔccnABCDE mutant relative to its isogenic ccn+ parent strain. The growth inhibition by Zn that occurs as the result of loss of the ccn genes is rescued by supplementation with Mn or Oxyrase, a reagent that removes dissolved oxygen. Lastly, we found that the Zn-dependent growth inhibition of the ΔccnABCDE strain was not altered by deletion of sodA, whereas the ccn+ ΔsodA strain phenocopied the ΔccnABCDE strain. Overall, our results indicate that the Ccn sRNAs have a crucial role in preventing Zn intoxication in S. pneumoniae.

Associations between multiple metals exposure and cognitive function in the middle-aged and older adults from China: A cross-sectional study

The rapidly rising risk of cognitive decline is a serious challenge for the elderly. As the wide-distributed environmental chemicals, the effects of metals exposure on cognitive function have attracted much attention, but the results remain inclusive. This study aimed to investigate the roles of multiple metals co-exposure on cognition. We included a total of 6112 middle-aged and older participants, detected their plasma levels of 23 metals by using inductively coupled plasma mass spectrometry, and assessed their cognitive function by using the Mini-Mental State Examination (MMSE). The results showed that increased plasma levels of iron (Fe) and zinc (Zn) were positively associated with MMSE score, but the increased levels of nickel (Ni) and lead (Pb) were associated with decreased MMSE score (all FDR < 0.05). Subjects exposed to both high levels of Ni and Pb showed the lowest MMSE score [β (95% CI) = −0.310 (−0.519, −0.100)], suggesting that Ni and Pb had a synergistic toxic effect on cognitive function. In addition, the hazardous roles of Ni and Pb were mainly found among subjects with low plasma level of Zn, but were not significant among those with high-Zn level [Ni: β (95% CI) = −0.281 (−0.546, −0.015) vs. −0.146 (−0.351, 0.058); Pb: β (95% CI) = −0.410 (−0.651, −0.169) vs. −0.060 (−0.275, 0.155)], which suggested that Zn could attenuate the adverse effects of Pb and Ni on cognitive function. The cognitive function was gradually decreased among subjects with increased number of adverse exposures to the above four metals (Ptrend < 0.001). In conclusion, our findings revealed the individual, interactive, and combined effects of Fe, Ni, Pb, and Zn on cognitive function, which may provide new perspectives on cognitive protection, but further prospective cohort studies and biological researches are needed to validate these findings.

Development of Iron and Zinc Biofortified Rice Variety for Nutrition Improvement in Bangladesh

Depleting micronutrients such as iron and zinc poses a serious risk to a population’s health in regions where nutrient-rich meals are unavailable, such as Bangladesh. We can engross the essential minerals using biofortified rice varieties enriched with essential minerals. We experimented to develop and evaluate a new high-yielding rice variety, Advanced Chemical Industries (ACI) dhan1, specifically for the rainy season in Bangladesh. We used high-yielding rice parent lines with high iron and zinc in the hybridization process, then grew and selected the resulting breeding lines during the rainy season of Bangladesh to identify the most suitable rice lines for the rainy season. The plant height and days to maturity traits were the same as shown in both cultivars, ACI dhan1 and Bangladesh Rice Research Institute (BRRI) dhan62 (standard check), which are 104 cm and 103 days, respectively. On the other hand, ACI dhan1 produced 6.05 tons per hectare, and BRRI dhan62 yielded 4.33 tons per hectare. A quality analysis of the grain quantified that ACI dhan1 contained 8.21% protein and 25.12% apparent amylose. Similarly, ACI dhan1 had very high levels of Zn (24.8 ppm) and Fe (9 ppm). We observed a more than 70% improvement in milling capabilities and head rice recovery. In proposed variety trials, the Seed Certification Agency of Bangladesh identified an average yield advantage of more than 10% for ACI dhan1 in 10 locations. National Seed Board, Bangladesh, released it as a candidate for high-iron and zinc-enriched rice variety and approved its extensive cultivation in Bangladesh. Due to its high micronutrient content, excellent agronomic performance, and non-sticky cooking quality, ACI dhan1 presents itself as a variety of choice in Bangladesh’s pursuit of food and nutrition security. Thus, the present study demonstrates the large achievement of biofortification by ACI dhan1 and hence promises to be a strong weapon in the battle against malnutrition.

Multi-Elemental Analysis of Edible Insects, Scorpions, and Tarantulas from French (Online) Market and Human Health Risk Assessment Due to Their Consumption: A Pilot Study.

Edible insects are becoming increasingly popular as protein alternatives to traditional animal-based products. As such, information on their elemental composition is important to ensure they are safe for human consumption. This article describes the development and validation of a rapid, reliable method for the simultaneous determination of 19 elements (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Pb, Se, Sr, and Zn) in edible insects by inductively coupled plasma mass spectrometry (ICP-MS) following closed vessel microwave digestion. The method was validated using three insect certified reference materials, namely black soldier fly larvae meal (BFLY-1), cricket flour (KRIK-1), and mealworm powder (VORM-1). The method was applied to analyze twelve different (whole) insect species. The maximum amount of each sample was calculated for As, Cd, and Pb with respect to their provisional tolerable daily intake values established by the Food and Agricultural Organization/World Health Organization. Most of the samples, except for scorpions and tarantulas, were safe to consume at large doses (1000-10,000 insects per day). Furthermore, most of the samples contained high levels of Fe, K, Na, and Zn, providing a preliminary overview of the nutritional profile of these novel protein alternatives.

Elemental Composition of the Leaves of the Hanging Birch (&lt;i&gt;Betula pendula&lt;/i&gt; Roth) in the Area of the Transbaikalia Gold Deposit

The influence of gold mining on the concentration of chemical elements in Betula pendula Roth leaves was studied. The intensity of absorption by the plant of a number of chemical elements, the possibility of using birch as a medicinal and forage plant, as well as for monitoring habitat changes were investigated. The study was conducted in plant communities located at production facilities and natural habitats in the area of the Baley gold deposit: tailings dumps of gold recovery factories (ZIF-1 and ZIF-2), a drainage landfill, a dump of the SredneGolgotai gold deposit, a tailings dump after processing monocytes, as well as in natural plant communities located in the vicinity of Baley at 13 trial sites in 2008, 2008 and 2021. The concentrations of elements in the leaves were arranged in descending order in the following sequence: Ca Mg P Mn Fe Zn Na Ba B Cr Ni As Cu Mo Sb Pb Co Li V Cd Bi Se Be. With respect to the clark of terrestrial plants, the concentrations of the elements were in the following order: Cr As Sb Li Ni Ba Fe Mo Bi Co Zn Mg Clark Mn P Ca Cu B Pb Se Cd V Be Na. The absorption of As at production facilities exceeded the norm established for medicinal plant raw materials. The use of branch feed in a number of areas is not allowed due to the high level of Zn, Fe, Sb, Ni, Cr, Co, As, Cd. A relatively high coefficient of biological absorption by birch leaves Zn, Mn, P, Ca, Mg was obtained.

Bioaccumulation of trace elements in marine mammals: New data and transplacental transfer on threatened species

Coastal areas are affected by urban, industrial and agriculture pollutants runoff, wastewater and stormwater discharges, making this environment the final repository of chemical contaminants. These contaminants have the potential to spread out to the entire food chain, impacting marine life and the quality of their habitat. In this aspect, the concept of marine mammals as bioindicators provides an approach to the degree of contamination in the environment and to the identification and management of multiple sources of contaminants. The present study analyzed several elements like As, Ba, Cd, Cr, Cu, Hg, Mn, Mo, Ni, Pb, V and Zn in liver tissue from two dolphin species: Sotalia guianensis, a near-threatened species, and the vulnerable Pontoporia blainvillei. In the study, we also investigated if dolphins (population) recorded using the heaviest urban areas have higher concentrations of contaminants in their tissues. Dolphin samples (n = 40 S. guianensis; n = 97 P. blainvillei) were collected by daily monitoring carried out by Santos Basin Beach Monitoring Project (PMP-BS), from stranded individuals found in São Paulo state. The Spearman's rank correlation showed distinct correlations in the accumulation of trace elements by both species, indicating different sources of exposure to the elements studied or distinct biochemical processes between species. Interspecific and intraspecific variations were observed, possibly related to the individual distribution and feeding habits. Correlations were observed between age and concentrations of trace elements, positive for Cd, Hg and Mo. Finally, our findings indicate high levels of Cu, Zn, and concentrations of As, V and Hg in fetuses, in particular, an analysis was performed on a fetus found inside a stranded individual, indicating placental transfer as the first route of exposure for some elements.

Study on the impact of adopting organic practices on the absorption and extractability of Cu and Zn in commercial coffee samples

Although coffee is not a primary source of minerals in the diet, due to its frequent consumption, it can be considered an important source of some micronutrients, highlighting those with antioxidant properties such as Cu and Zn. The availability of nutrients in foods consumed through infusion is related to solubility, which is influenced by the metabolization of these elements by the plant. Several factors can impact this metabolism, including cultivation. This study investigated the influence of cultivation on the absorption and extractability of Cu and Zn in conventional and organic commercial coffee. It was observed that cultivation influences the absorption of the minerals studied in opposite ways: while organic samples have higher levels of Zn, conventional samples have higher levels of Cu. Cultivation does not influence the extractability of Zn in coffee infusions, therefore indicating little influence on the metabolization of this element. Copper metabolism, on the other hand, is influenced by cultivation, with Cu exhibiting greater affinity and lower extractability in organic coffee samples compared to conventional ones.

Comparative transcriptomics provides insight into molecular mechanisms of zinc tolerance in the ectomycorrhizal fungus Suillus luteus.

Zinc (Zn) is a major soil contaminant and high Zn levels can disrupt growth, survival, and reproduction of fungi. Some fungal species evolved Zn tolerance through cell processes mitigating Zn toxicity, though the genes and detailed mechanisms underlying mycorrhizal fungal Zn tolerance remain unexplored. To fill this gap in knowledge, we investigated the gene expression of Zn tolerance in the ectomycorrhizal fungus Suillus luteus. We found that Zn tolerance in this species is mainly a constitutive trait that can also be environmentally dependent. Zinc tolerance in S. luteus is associated with differences in expression of genes involved in metal exclusion and immobilization, as well as recognition and mitigation of metal-induced oxidative stress. Differentially expressed genes were predicted to be involved in transmembrane transport, metal chelation, oxidoreductase activity, and signal transduction. Some of these genes were previously reported as candidates for S. luteus Zn tolerance, while others are reported here for the first time. Our results contribute to understanding the mechanisms of fungal metal tolerance and pave the way for further research on the role of fungal metal tolerance in mycorrhizal associations.

Zinc phytotoxicity and cationic micronutrients contents in quinoa as influenced by high Zn levels and soil amendments

Contamination of soils with heavy metals (HMs) is a serious concern due to the non-biodegradability and accumulation of these metals in living organism. The aim of this study was to evaluate the effectiveness of fish scale powder, chitin, and chitosan in reducing zinc (Zn) accumulation of quinoa grown on Zn-polluted soil. A 3*4 factorial greenhouse trial was conducted with three levels of Zn contamination (0, 100, and 200 mg kg−1) and four types of amendment (control, and 0.15% of each fish scale powder, chitin, and chitosan). The quinoa seeds were sown in soil treated with Zn contamination and amendments. Results showed that application of amendments especially chitosan increased shoot and root dry weight and greenness index of quinoa leaf. While high soil Zn levels decreased cationic micronutrients uptake in quinoa shoot, chitosan addition desirably enhanced cationic micronutrients uptake in quinoa shoot. Overall, amendment addition significantly decreased Zn concentration and uptake in plant; the highest decrease was observed following the application of chitosan. Although the application of high Zn levels significantly increased the values of phytoextraction efficiency, application of chitin and chitosan significantly decreased it. The value of translocation factor was lower than one, demonstrating that a large amount of Zn was stabilized in the root tissues, while a small amount of this metal was transferred to aboveground parts of quinoa. Results of this study revealed that fish scales derivatives were effective in preventing Zn toxicity and at the same time increasing cationic micronutrients contents of quinoa grown on HMs-polluted soil.

Fate of antibiotic resistance genes and EPS defence mechanisms during simultaneous denitrification and methanogenesis, coupled with the biodegradation of multiple antibiotics under zinc stress

High-strength nitrogen and antibiotics-containing wastewater can be efficiently eliminated by simultaneous denitrification and methanogenesis (SDM). Heavy metals and antibiotics are two critical factors that can lead to horizontal transfer of antibiotic resistance genes (ARGs), which can be simultaneously detected in wastewater. Unfortunately, the impacts of heavy metals on SDM and antibiotic biodegradation have not been fully elucidated. Herein, the effects of SDM and multiple antibiotics biodegradation, extracellular polymeric substances (EPSs) and protein response mechanisms, and ARG fate under Zn(II) stress were comprehensively evaluated. The results indicated that a high level of Zn(II) (≥5 mg/L) stress significantly decreased the degradation rate of multiple antibiotics and suppressed denitrification and methanogenesis. In addition, Zn(II) exposure prompted the liberation of proteins from microbes into the EPSs, and the combination of EPSs with small molecules quenched the original fluorescent components and destroyed the protein structure. The dominant proteins can bind to both Zn(II) and multiple antibiotics through several types of chemical interactions, including metallic and hydrogen bonds, hydrophobic interactions, and salt bridges, relieving the toxicity of harmful substances. Moreover, metagenomic sequencing revealed that the abundance of zinc resistance genes (Zn-RGs), ARGs (mainly tetracyclines), and mobile genetic elements (MGEs) increased under Zn(II) stress. Mantel test illustrated that the ARGs mecD, tetT, and tetB(60) were most affected by MGEs. Moreover, molecular network analysis revealed that several MGEs can bridge metal resistance genes (MRGs) and ARGs, facilitating the horizontal transfer of ARGs. This study provides theoretical guidance for the environmental risk control of antibiotics-containing wastewater treated by an SDM system.

Optimization and validation of a simple extraction method coupled with ICP-MS for the determination of essential and toxic elements in liquid dietary supplement products

This study aimed to develop and validate a fast, easy, cost-effective, and accurate method for determining Mn, Co, Ni, Cr, Cu, Fe, Zn, As, Cd, Hg, Sb, and Pb in liquid dietary supplements using an inductively coupled plasma mass spectrometer (ICP-MS). Two sample preparation methods were compared: microwave acid digestion and direct analysis after dilution. Both methods provided acceptable recovery values (87.7–111 %), with RSD values up to 6.35 %. The microwave digestion method was validated using various certified reference materials (CRMs) and a proficiency test (PT). Additionally, 34 domestic dietary supplements were analyzed using both methods, and the results were similar. Only one sample exceeded the maximum allowable limit for arsenic, which is 150 mg/kg set by the United States Pharmacopeia (USP). Two products had high Fe and Zn levels exceeding the recommended daily allowance (RDA), according to the Food and Drug Administration (FDA). This study demonstrates the effectiveness and advantages of the direct analysis method for measuring essential and toxic elements in liquid dietary supplements. This method promotes green chemistry principles and provides valuable information for ensuring consumer safety and product quality.

Geochemical signature identifying features and archaeological structures in eastern Amazonian Terra Preta sites

AbstractThe present work describes the chemistry and mineralogy of soils such as Terra Preta Arqueológica (TPA) or Terra Preta de Índio (TPI) from the Bitoca I and II sites, located in the Salobo Region (Carajás Mineral Province, Pará‐Brazil). The results revealed chemical and mineralogical characteristics that are similar to other TPAs found throughout the Amazon region, such as relatively high levels of Ca (average of 3600 ppm), P (average of 850 ppm), Mn (average of 730 ppm), Zn (average of 55 ppm), and Cu (average of 63 ppm). In soils related to the characteristics of occupation by huts and campfires, the mineralogical composition is represented by quartz, kaolinite, calcite, muscovite, anatase, hematite, goethite, and gibbsite. Amorphous phases have also been identified mainly as calcium phosphates and organic matter (humic and fulvic acids). The analysis of the features/structures described here helped in the identification/verification of areas destined for different activities within the sites.

Differentiating anthropogenic effects from natural metal(loid) levels in residential soil near a zinc smelter in South Korea.

Metal(loid)s pose a significant hazard due to inherent toxicity. Individuals are particularly exposed to metal(loid)s in soil through direct or indirect contact. Identifying metal(loid) sources in soil is required for exposure mitigation to anthropogenic metal(loid)s, while metal(loid)s are natural constitutes of soil. Metal(loid) concentrations and Pb isotopes were determined in residential soil profiles impacted by a Zn smelter to distinguish the anthropogenic effect from natural levels. One hundred sixty-nine core soil samples were collected from depths down to 5.5m below ground level at 19 sites and were divided into Zn-Cd-As- and As-contaminated groups based on the worrisome level (WL) of soil contamination. The Zn-Cd-As-contaminated group (n = 62) was observed at depths < 1m, showed high Zn levels (mean of 1168mg/kg) and Cd and As frequently exceeding WLs, and had low 206Pb/207Pb ratios close to the Zn smelter. In contrast, the As-contaminated group (n = 96) was observed at depths > 1m, did not have other metals exceeding WLs, and showed a wide range of 206Pb/207Pb ratios far away from the Zn smelter. The results indicated that the pollution sources of Zn-Cd-As- and As-contaminated soils were fugitive dust emissions from smelter stacks and geology, respectively. The metal(loid)s in host rock set geochemical baselines in soil profiles, while smelting activities affected the upper layers over 50years. This study demonstrated the effectiveness of utilizing the vertical distribution of metal(loid) concentrations and Pb isotopes in soil profiles for distinguishing between anthropogenic and geogenic origins, in combination with baseline assessment.

Comparison of zinc levels in mothers with and without abortion: A systematic review and meta-analysiss

IntroductionZinc is an essential trace element involved in different physiological functions. During pregnancy, it plays a crucial role in healthy embryogenesis. Abortion is the most severe problem associated with early pregnancy complications. This study aims to compare the levels of Zn in mothers with and without abortion. MethodsThis study is a systematic review and meta-analysis of studies published between 1980 and 2022 in PubMed, Science Direct, Pro Quest, Wiley, Web of Science, and Scopus databases. The search was conducted using both main and Mesh keywords, specifically targeting terms related to abortion, pregnancy loss, and zinc. Heterogeneity in the studies included in the meta-analysis was evaluated using statistical tests such as the chi-square test, I2, and forest plots. Publication bias was assessed using Begg's and Egger's tests. All analyses were conducted using Stata 15, and statistical significance was considered at p < 0.05. ResultsIn general, nine studies were included in this analysis. Based on the results of the meta-analysis, the mean difference in the Zn level between mothers with and without abortion is equal to 193.18 (95 % CI; 107.11 to 279.25, P ≤ 0.001). In other words, the level of Zn in the group of mothers without abortion is 193 units higher than the group of mothers with abortion. The investigation into the comparison of Zn levels in mothers with and without abortion did not reveal any indication of publication bias according to both Begg's test (P-value = 0.858) and Egger's test (P-value = 0.270). Conclusionthe study findings indicate a significant association between lower levels of Zn in mothers with abortion compared to mothers without abortion. This suggests that a higher level of Zn may have a preventive effect on the occurrence of abortion in pregnant women. The data also highlights the positive role of trace metals, particularly Zn, in influencing pregnancy outcomes and suggests that maintaining adequate levels of Zn may reduce the likelihood of abortion occurrence, along with other contributing factors. It is important to note that further research, including prospective cohort and experimental studies, is needed to provide more substantial evidence and strengthen these findings.

Effects of phosphorus and zinc levels on total soluble carbohydrate and crude protein in grain of cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp) grown in Bauchi, Nigeria

Two field experiments were conducted at the Faculty of Agriculture and Agricultural Engineering Research Farm of Abubakar Tafawa Balewa University, Bauchi, between 2006 and 2007 to study the effects of phosphorus (0, 25, 50 kg P/ha) and zinc levels (0, 2.5, 5 kg Zn/ha) on Total Soluble Carbohydrate and Crude Protein of six cowpea varieties namely: IT90K 277, IT93455 1, IT89KD 288, IT97K 568 18, IT90K 82 2 and Kanannado. The objectives of these experiments were to determine which levels of P, Zn and the various interactions of P, Zn, and year that had produced the highest total soluble carbohydrate (TSC) and crude protein (CP) on the grain contents of the cowpea varieties studied and to determine the best varieties in terms of TSC and CP upon the application of these nutrients (P and Zn) in Bauchi, Nigeria. The results revealed that increased levels of P from P0 to P50 significantly increased the CP and TSC contents of the cowpea grains. Similarly, higher Zn levels (2.5 and 5 kgha-1) were observed to have significant effects on TSC and CP contents of the cowpea grains. Interactions of P and Zn were equally observed to significantly affect TSC and CP contents of the cowpea grains. Of all the six (6) cowpea varieties, Kanannado recorded the highest TSC and CP in the cowpea grains. With regard to year, 2006 recorded the highest CP while 2007 had the highest TSC. Higher levels of P and Zn or their associations were recommended for improving TSC and CP contents of the cowpea grains, with emphasis on Kanannado for Bauchi farmers.