We developed the playback control software for a high-speed playback system which is a component of the Korea-Japan Joint VLBI Correlator (KJJVC). The Mark5B system, which is a recorder and playback system used in the Korean VLBI Network (KVN), has two kinds of operation mode. That is to say, the station unit (SU) mode, which is for the present Mark4 system, and the VSI mode, which is for the new VLBI standard interface (VSI) system. The software for SU is already developed and widely used in the Mark4 type VLBI system, but the software for VSI has only been developed for recording. The new VLBI system is designed with a VSI interface for compatibility between different systems. Therefore, the playback control software development of the VSI mode is needed for KVN. In this work, we developed the playback control software of the Mark5B VSI mode. The developed playback control software consists of an application part for data playing back, a data input/output part for the VSI board, a module for the StreamStor RAID board, and a user interface part, including an observation time control part. To verify the performance of developed playback control software, the playback and correlation experiments were performed using the real observation data in Mark5B system and KJJVC. To check the observation time control, the data playback experiment was performed between the Mark5B and Raw VLBI Data Buffer (RVDB) systems. Through the experimental results, we confirmed the performance of developed playback control software in the Mark5B VSI mode.