The quality of mother-infant interaction directly impacts neonatal and maternal health and well-being. Nurses play a significant role in facilitating and supporting the mothers to participate in the care of their neonates admitted to Neonatal Intensive Care Unit (NICU). Capacity-Building Program (CBP) was conducted to empower the neonatal nurses to facilitate and support mothers in routine care of their neonates admitted to NICU. A total of 53 neonatal nurses participated in the program. This program focused on empowering the neonatal nurses on the responsibilities and strategies to involve mothers in routine care of neonates at NICU. The program was evaluated by testing nurses’ knowledge through pre-test and post-test score analysis. Neonatal nurses demonstrated a higher level of knowledge post-implementation of the program. It was found that the capacity-building program intervention was effective (p < 0.001) in improving the knowledge of neonatal nurses on the role of nurses in facilitating and supporting mothers in the care of their neonates admitted to NICU.