High-resolution Probe Research Articles

Insights into the Structure and Dynamics of Proteins from 19F Solution NMR Spectroscopy.

19F NMR spectroscopy has recently witnessed a resurgence as an attractive analytical tool for the study of the structure and dynamics of biomolecules in vitro and in cells, despite reports of its applications in biomolecular NMR since the 1970s. The high gyromagnetic ratio, large chemical shift dispersion, and complete absence of the spin 1/2 19F nucleus from biomolecules results in background-free, high-resolution 19F NMR spectra. The introduction of 19F probes in a few selected locations in biomolecules reduces spectral crowding despite its increased line width in comparison to typical 1H NMR line widths and allows rapid site-specific measurements from simple 1D spectra alone. The design and synthesis of novel 19F probes with reduced line widths and increased chemical shift sensitivity to the surrounding environment, together with advances in labeling techniques, NMR methodology, and hardware, have overcome several drawbacks of 19F NMR spectroscopy. The increased interest and widespread use of 19F NMR spectroscopy of biomolecules is gradually establishing it as a sensitive and high-resolution probe of biomolecular structure and dynamics, supplementing traditional 13C/15N-based methods. This Review focuses on the advances in 19F solution NMR spectroscopy of proteins in the past 5 years, with an emphasis on novel 19F tags and labeling techniques, NMR experiments to probe protein structure and conformational dynamics in vitro, and in-cell NMR applications.

Toward utilizing scanning gate microscopy as a high-resolution probe of valley splitting in Si/SiGe heterostructures

Toward utilizing scanning gate microscopy as a high-resolution probe of valley splitting in Si/SiGe heterostructures

Advances in Genotyping Detection of Fragmented Nucleic Acids.

Single nucleotide variant (SNV) detection is pivotal in various fields, including disease diagnosis, viral screening, genetically modified organism (GMO) identification, and genotyping. However, detecting SNVs presents significant challenges due to the fragmentation of nucleic acids caused by cellular apoptosis, molecular shearing, and physical degradation processes such as heating. Fragmented nucleic acids often exhibit variable lengths and inconsistent breakpoints, complicating the accurate detection of SNVs. This article delves into the underlying causes of nucleic acid fragmentation and synthesizes the strengths and limitations of next-generation sequencing technology, high-resolution melting curves, molecular probes, and CRISPR-based approaches for SNV detection in fragmented nucleic acids. By providing a detailed comparative analysis, it seeks to offer valuable insights for researchers working to overcome the challenges of SNV detection in fragmented samples, ultimately advancing the accurate and efficient detection of single nucleotide variants across diverse applications.

Ultrasound nail assessment in patients with psoriasic arthritis: is there an association of findings with clinical scores?

BackgroundPsoriatic arthritis can involve several domains. Due to its multifaceted nature and its frequent comorbidities such as depression, obesity, osteoarthritis and fibromyalgia, it is difficult to monitor these patients because the clinical scores involve subjective data. High-resolution ultrasound probes allowed the evaluation of more superficial structures, such as the nails and their synovio-entheseal framework, in close relationship with the enthesis of the distal extensor digitorum tendon. Nail ultrasound studies vary in terms of the parameters and fingers studied and in their findings.ObjectivesTo describe the most significant sonographic nail changes and the most affected fingers in psoriatic arthritis and to verify the association of nail ultrasound findings with clinical scores (nail psoriasis severity index (NAPSI), ankylosing spondylitis disease activity score with C-reactive protein (ASDAS-CRP), minimal disease activity (MDA), disease activity index for psoriatic arthritis (DAPSA)).MethodsThis was a cross-sectional study with 52 patients with psoriatic arthritis at the Hospital de Clínicas do Paraná and 50 controls. A total of 1016 nails were analyzed (517 from patients with psoriatic arthritis and 499 from controls). Ultrasonography of the nails of the 10 fingers was performed to assess the trilaminar appearance, measure the distance from the nail bed, identify synovitis of the distal interphalangeal joints and the presence of a power Doppler signal from the nail matrix/nail bed. The captured images were independently evaluated by a rheumatologist with expertise in musculoskeletal ultrasound. Data analysis was performed using IBM SPSS Statistics v.28.0.0 software, and the association of nail plate changes, nail bed distance and power Doppler signal with the NAPSI, DAPSA, MDA and ASDAS-PCR were calculated. Spearman correlation coefficients were estimated to analyze the correlations between pairs of quantitative variables. Student’s t test and the Mann‒Whitney U test were used to compare quantitative variables, and Fisher’s exact test was used to compare categorical variables between patients and controls. The nonparametric Mann‒Whitney U and Kruskal‒Wallis tests were used to compare groups according to the MDA or DAPSA classification.ResultsThe Doppler signal of the nail matrix and nail bed was more frequently identified in patients (44.2%) than in controls (6%), and the difference in the mean power Doppler signal between the two groups was significant (p < 0.001). Changes in the nail plate were more common in the right thumb (44.2%), left thumb (36.5%) and second finger on the right hand (32.7%). The number of fingers with nail plate changes, enthesitis, paratendinitis, grayscale synovitis and DIP involvement in the distal interphalangeal joints was higher among patients with psoriatic arthritis (p < 0.001). There were found some correlations between US findings and clinical scores: ultrasound nail involvement and the NAPSI score (p = 0.034), the number of fingers and mean change in the nail plate and the ASDAS-CRP (p = 0.030). DAPSA (remission/low activity versus moderate/high activity) was associated to the mean change in the nail plate (p < 0.013). CONCLUSIONS: Nail ultrasound has the potential to assist in the capturing of the actual disease activity status in patients with psoriatic arthritis.

Computational Characterization of the On-Surface Synthesis of Low-Dimensional Nanostructures

On-surface chemistry represents a fast-growing field allowing to synthesize molecular structures not available by traditional wet chemistry. In this context, high-resolution scanning probe microscopy techniques are able to provide unprecedented spatial resolution, allowing to precisely identify individual products of the reactions. Nevertheless, a deep understanding of the reaction mechanism under the conditions imposed by the substrate remains unknown.Nowadays, various computational strategies are employed to characterize the optimal reaction pathways of the chemical processes that take place on the surfaces of solids. For example, one of the most popular approaches is the nudged elastic band (NEB) as well as different transition state theory methods beyond it. These approaches had demonstrated their capability to characterize the potential energy landscape of the reaction pathways [1-2]. Another approach that had attracted the interest of the on-surface synthesis community is the application of molecular dynamics (MD) methods, within the canonical ensemble, and based on the QM/MM (quantum mechanics/molecular mechanics) of the interatomic forces [3-4]. This approach, which combines Density functional theory (DFT) and empirical force fields to describe the interaction forces, allows to simulate very large systems, at a very low computational cost while still accounting for the crucial quantum mechanics interactions. Also, such temperature-dependent simulations include the effect of entropy, vibrations modes, concerted motion, etc.I will present the experimental and computational results of different projects in which we have made use of the aforementioned computational approaches to achieve a better understanding of the on-surface synthesis experiments performed by the group. For example, the rationalization of the selective cyclodehydrogenation of non-benzenoid nanographenes deposited on a Au(111) substrate by means of temperature-dependent MD calculations and the analysis of the obtained vibrational modes (See Figure 1a). The group had also studied the stepwise transformation of coronene-based organometallic networks into two-dimensional covalent patches through intermolecular homocoupling (See Figure 1b). We had as well investigated the transformation of indenyl precursors, through a triple C-H cleavage followed by a peripheral directed homocoupling, to form a polyacetylene backboned polymer that features a parity dependent and highly delocalized soliton in-gap state (See Figure 1c).

On-Surface Synthesis of Macrocycles, Non-Planar Carbon Ribbons and Heteroatom-Doped Nanographenes

On-surface synthesis via covalent coupling of adsorbed molecules on metal surfaces has attracted significant attention recently due to its potential to fabricate low-dimensional carbon materials with atomic precision. The bottom-up, atomically precise synthesis of carbon nanostructures enables the tailoring of their electronic properties at a molecular level. To understand and control the surface-chemistry-driven synthesis, many efforts have been made to design innovative precursors, explore novel reaction schemes, and utilize templating effects from the substrate.My presentation focuses on high-resolution scanning probe microscopy experiments combined with density functional theory to demonstrate recent highlights on the assembly of surface-supported low-dimensional carbon structures on metal surfaces. First, the assembly and electronic structure of planar π-extended cycloparaphenylene macrocycles, representing the first nanographene with an all-armchair edge topology, will be presented [1]. The second part will discuss the bottom-up synthesis of covalently-linked non-planar carbon ribbons and their electronic properties depending on their adsorption geometry. Finally, I will conclude with the on-surface cyclomerization of oxygen heterocycles to understand the on-surface synthesis of furan and pyran derivatives from ketone-functionalized precursors on metal surfaces.[1] Xiang, et al. Nature Chem., 2022 14, 871–876.

Syntheses of Heteroatom-Substituted and Three-Dimensional Nanocarbon Materials on Surfaces

I will present our recent studies on on-surface chemistry with high-resolution scanning probe microscopy operating at low temperature under ultra-high vacuum. We synthesized three-dimensional organometallic compound and graphene nanoribbon by coupling hexabromo substituted-propellane molecules on Au(111) and Ag(111).[1] In the structure, the C-Br bonds distant from the surface remained intact even after the reaction. The radical species were formed by tip-induced debromination and were also stabilized by either tip-manipulated Br atom or fullerene molecule. We also demonstrate systematic tip-induced isomerization via embedding a text of “NIMS” by following binary and ternary ascii cords, in which two chiral dehydroazulene and diradical units were used.[2] Among the three states, the diradical unit showed the spin coupling of 90 meV. For heteroatom substitution, we developed an on-surface reaction, which can synthesize graphene nanoribbon and covalent organic frameworks with silabenzene units by coupling Si atom and Br-substituted molecule.[3] The heavier congeners of cyclic aromatic compounds have been studied as an elusive target product for organic synthesis due to their high reactivity at ambient temperature and difficult isolation. Thus, this result shows the advantage of on-surface synthesis.Reference[1] S. Kawai, et al Sci. Adv. 6, eaay8913 (2020).[2] S. Kawai, et al Nat. Commun. 14, 7741 (2023).[3] K. Sun et al, Nat. Chem. 15, 136 (2023).

ETMR-11. RECURRENT GENETIC ALTERATIONS IN EPIGENETICALLY DEFINED PINEOBLASTOMA SUBTYPES

Abstract INTRODUCTION Genome-wide methylation analyses recently revealed distinct epigenetic pineoblastoma (PBL) subtypes. The aim of the study was to shed further light onto the genetic characteristics underlying the pathogenesis of pineoblastoma subtypes. METHODS Cytogenetic alterations of tumor samples of 76 patients with a diagnosis of PBL confirmed by reference neuropathology and methylation-based subtyping were analyzed by high-resolution genome-wide molecular inversion probe analysis. Seventy-two cases were screened for mutations by next-generation DNA panel sequencing. Survival data of 49 patients were available. Patients ≥4y received postoperative RT followed by maintenance chemotherapy (n=42); infants (&amp;lt;4y) were primarily treated on RT-sparing regimens with intensified chemotherapy. RESULTS According to epigenetic consensus PBL subtypes (Liu et al., 2021), our cohort consisted of 48 PBL-miRNA1 (1A=40; 1B=8), 17 PBL-miRNA2, 4 PBL-MYC/FOXR2, and 7 PBL-RB1 samples. PBL-miRNA subtype tumors had characteristic alterations in microRNA-processing genes; DICER1 mutations (n=18) and homozygous deletions of the DROSHA locus (n=17) were most abundant, followed by DROSHA mutations (n=12). Most frequent cytogenetic aberrations in PBL-miRNA were whole chromosome (chr.) 7 gain (n=30) and chr. 14 loss (n=21). DICER1 mutations were significantly associated with chr. 14 loss (p&amp;lt;0.001). Twenty-one cases showed gains of chr. 14, 8 additional cases gains of the OTX2 oncogene (chr. 14q). In the miRNA subtypes we identified cases with polyploid cytogenetics (n=15). Of note, only one of the 8 patients with polyploid tumors and survival data died, while the remaining patients of the miRNA subtypes had a 5-year PFS/OS of 61.46±9.27/63.24±9.23% (14 of 35 died) after a median follow-up time of 5.52 years. However, this comparison did not reach statistical significance (p=0.25). CONCLUSION The epigenetically defined PBL-miRNA subtypes are characterized by distinct cytogenetic and mutational events. A possible prognostic role for a superior (e.g. polyploidy) or inferior outcome requires investigation in prospective clinical trials.

High-resolution spectroscopic probing of allowed and forbidden ortho- and para-nuclear spin-isomers of NH3

High-resolution spectroscopic probing of allowed and forbidden ortho- and para-nuclear spin-isomers of NH3

Prospective measurement of the width of cerebrospinal fluid spaces by cranial ultrasound in neurologically healthy children aged 0–19 months

BackgroundUltrasound (US) is often the first method used to look for brain or cerebrospinal fluid (CSF) space pathologies. Knowledge of normal CSF width values is essential. Most of the available US normative values were established over 20 years ago, were obtained with older equipment, and cover only part of the age spectrum that can be examined by cranial US. This prospective study aimed to determine the normative values of the widths of the subarachnoid and internal CSF spaces (craniocortical, minimal and maximal interhemispheric, interventricular, and frontal horn) for high-resolution linear US probes in neurologically healthy infants and children aged 0–19 months and assess whether subdural fluid collections can be delineated.MethodsTwo radiologists measured the width of the CSF spaces with a conventional linear probe and an ultralight hockey-stick probe in neurologically healthy children not referred for cranial or spinal US.ResultsThis study included 359 neurologically healthy children (nboys = 178, 49.6%; ngirls = 181, 50.4%) with a median age of 46.0 days and a range of 1–599 days. We constructed prediction plots, including the 5th, 50th, and 95th percentiles, and an interactive spreadsheet to calculate normative values for individual patients. The measurements of the two probes and the left and right sides did not differ, eliminating the need for separate normative values. No subdural fluid collection was detected.ConclusionNormative values for the widths of the subarachnoid space and the internal CSF spaces are useful for evaluating intracranial pathology, especially when determining whether an increase in the subarachnoid space width is abnormal.

Different scenarios in sloshing flows near the critical filling depth

In the present paper, the sloshing flow in a cuboid tank forced to oscillate horizontally is investigated with both experimental and numerical approaches. The filling depth chosen is $h/L=0.35$ (with h the water depth and L the tank height), which is close to the critical depth. According to Tadjbakhsh &amp; Keller (J. Fluid Mech., vol. 8, issue 3, 1960, pp. 442–451), as the depth passes through this critical value the response of the resonant sloshing dynamics changes from ‘hard spring’ to ‘soft spring’. The experimental tank has a thickness of $0.1L$ , reducing three-dimensional effects. High-resolution digital camera and capacitance wave probes are used for time recording of the surface elevation. By varying the oscillation period and the amplitude of the motion imposed on the tank, different scenarios are identified in terms of free-surface evolution. Periodic and quasi-periodic regimes are found in most of the frequencies analysed but, among these, sub-harmonic regimes are also identified. Chaotic energetic regimes are found with motions of greater amplitude. Typical tools of dynamical systems, such as Fourier spectra and phase maps, are used for the regime identification, while the Hilbert–Huang transform is used for further insight into doubling-frequency and tripling-period bifurcations. For the numerical investigation, an advanced and well-established smoothed particle hydrodynamics method is used to aid the understanding of the physical phenomena involved and to extend the range of frequencies investigated experimentally.

Thermal scanning probe and laser lithography for patterning nanowire based quantum devices

Semiconductor nanowire (NW) quantum devices offer a promising path for the pursuit and investigation of topologically-protected quantum states, and superconducting and spin-based qubits that can be controlled using electric fields. Theoretical investigations into the impact of disorder on the attainment of dependable topological states in semiconducting nanowires with large spin–orbit coupling and g-factor highlight the critical need for improvements in both growth processes and nanofabrication techniques. In this work, we used a hybrid lithography tool for both the high-resolution thermal scanning probe lithography and high-throughput direct laser writing of quantum devices based on thin InSb nanowires with contact spacing of 200 nm. Electrical characterization demonstrates quasi-ballistic transport. The methodology outlined in this study has the potential to reduce the impact of disorder caused by fabrication processes in quantum devices based on 1D semiconductors.

Energy fluxes and vertical heat transfer in the Southern Baltic Sea

IntroductionThis study investigates the dynamics of energy fluxes and vertical heat transfer in the Southern Baltic Sea, emphasizing the significant role of the dicothermal layer in modulating the penetration of the thermocline and the propagation rates of thermal energy. The research aims to elucidate the complex patterns of solar energy absorption, its conversion into sea surface temperature (SST), and the transference of this energy deeper into the marine environment.MethodsData were collected through 93 monitoring cruises by the Institute of Oceanology of the Polish Academy of Sciences (IOPAN) from 1998 to 2023, using a high-resolution towed probe technique alongside Argo floats data for the Baltic Proper from 2020 to 2023. ERA5 climate reanalysis dataset and NEMOv4.0 ocean model forecasts were also utilized for a comprehensive analysis of VITE, Top Net Short-Wave Radiation, SST, and energy budget across the Southern Baltic Sea.Results and discussionThe Southern Baltic Sea functions as a net energy sink, with an average energy budget of 5.48 W m-2, predominantly absorbing energy during daylight and emitting it from September to February. A 59-day lag between peak solar energy and VITE peak was observed, followed by an additional 6-day delay before peak SST. The study further reveals a 15-day delay in temperature phase shift per 10 meters depth due to the dicothermal layer's influence on thermal energy propagation, extending to 35 days in the Central and Northern Baltic. Heat transfer is significantly affected by the levels of the thermocline and halocline, with regional variations in advection-driven seasonal signals. The pronounced thermal inertia and the critical role of the dicothermal layer underscore the complexity of thermal energy distribution in the Southern Baltic Sea.

Intergranular corrosion of Ni-30Cr in high-temperature hydrogenated water after removing surface passivating film

High-resolution transmission electron microscopy and atom probe tomography are used to characterize the initial passivation and subsequent intergranular corrosion of degraded grain boundaries in a model Ni-30Cr alloy exposed to 360 °C hydrogenated water. Upon initial exposure for 1000 h, the alloy surface directly above the grain boundary forms a thin passivating film of Cr2O3, protecting the underlying grain boundary from intergranular corrosion. However, the metal grain boundary experiences severe Cr depletion and grain boundary migration during this initial exposure. To understand how Cr depletion affects further corrosion, the local protective film was sputtered away using a glancing angle focused ion beam. Upon further exposure, the surface fails to repassivate, and intergranular corrosion is observed through the Cr-depleted region. Through this combination of high-resolution microscopy and localized passive film removal, we show that, although high-Cr alloys are resistant to intergranular attack and stress corrosion cracking, degradation-induced changes in the underlying metal at grain boundaries make the material more susceptible once the initial passive film is breached.

Solid-State 183W NMR Spectroscopy as a High-Resolution Probe of Polyoxotungstate Structures and Dynamics.

Polyoxometalates such as ammonium paratungstate (APT) constitute an important class of metal oxides with applications for catalysis, (opto)electronics, and functional materials. Structural analyses of solid polyoxometalates mostly rely on X-ray or neutron diffraction techniques, which are largely limited to compounds that can be isolated with long-range crystallographic order. While 183W NMR has been shown to probe polyoxotungstate structures and dynamics in solution, its application to solids has been extremely limited. Here, state-of-the-art methods for the detection of solid-state 183W NMR spectra are tested and compared for APT in different hydration states. The highly resolved solid-state spectra distinguish each crystallographically distinct site in the tungstate structure. Furthermore, the 183W chemical shifts are shown to be highly sensitive to the local structure, dynamics, and symmetry of APT, establishing solid-state 183W NMR spectroscopy as a potent probe for analysis of polyoxotungstates and other tungsten-derived materials to complement solution NMR and diffraction-based techniques.

High resolution magnetic field imaging probe with two rows of TMR sensors for small defects inspection

It is still a challenging problem to detect small defects for eddy current array probes, which requires the probes to possess excellent sensitivity, as well as high spatial resolution. This paper presents a novel high-resolution magnetic field imaging probe with two rows of tunneling magnetoresistance (TMR) array sensors. The bare die sensors are integrated on a printed circuit board by golden wire bonding technology. The two rows of sensors are placed staggered with each other. The data of the two arrays are merged into a matrix, in which way the image pixel pitch is increased to 0.25 mm. The probe employs a differential scheme to suppress the noise, so as to detect the weak signal of small defects. To highlight the weak defect indications, feature extraction and segmentation algorithms are developed. The experimental results confirm that the proposed method can inspect a small defect with dimensions 1 mm (length) × 0.1 mm (width) × 0.1 mm (depth) on a stainless-steel sample.

New Method for Precise Measurement of Clamping Plate Deformations on Forming Presses

The deformation of press bolster plates under load can significantly impact the quality of manufactured workpieces. Consequently, press manufacturers are usually obliged to provide the metric proof of the permissible deformation values during the press commissioning process. Unfortunately, the existing measurement methods for determining bolster plate deformations present flaws in the measurement accuracy, the application flexibility, the metrological effort, and other aspects. These issues have been addressed through the development of a new measurement method using multiple inclination profiles on the surface of the measured object. Based on the difference in the inclination between the unloaded and loaded states, the method approximates the inclination surface and calculates the deformation of the measured object through integration. The measurement method was first used for commissioning tests of forming presses. A comparison with the results obtained with a measurement frame equipped with high-resolution measurement probes demonstrates an accuracy of the new method of less than 20 µm.

Structural Analysis of the Hepatitis B Virus RNA Encapsidation Signal ε by Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE).

RNA structure is crucial for RNA function, including in viral cis-elements such as the hepatitis B virus (HBV) RNA encapsidation signal ε. Interacting with the viral polymerase ε mediates packaging of the pregenomic (pg) RNA into capsids, initiation of reverse transcription, and it affects the mRNA functions of pgRNA. As free RNA, the 61-nucleotide (nt) ε sequence adopts a bipartite stem-loop structure with a central bulge and an apical loop. Due to stable Watson-Crick base pairing, this was already predicted by early RNA folding programs and confirmed by classical enzymatic and chemical structure probing. A newer, high-resolution probing technique exploits the selective acylation of solvent-accessible 2'-hydroxyls in the RNA backbone by electrophilic compounds such as 2-methylnicotinic acid imidazolide (NAI), followed by mapping of the modified sites by primer extension. This SHAPE principle has meanwhile been extended to numerous applications. Here we provide a basic protocol for NAI-based SHAPE of isolated HBV ε RNA which already provided insights into the impact of mutations, and preliminarily, of polymerase binding on the RNA structural dynamics. While the focus is on NAI modification, we also briefly cover target RNA preparation by in vitro transcription, primer extension using a radiolabeled primer, and analysis of the resulting cDNAs by denaturing polyacrylamide gelelectrophoresis (PAGE). Given the high tolerance of SHAPE chemistry to different conditions, including applicability in live cells, we expect this technique to greatly facilitate deciphering the conformational dynamics underlying the various functions of the ε element, especially in concert with the recently solved three-dimensional structure of the free RNA.

Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing

RNA begins to fold as it is transcribed by an RNA polymerase. Consequently, RNA folding is constrained by the direction and rate of transcription. Understanding how RNA folds into secondary and tertiary structures therefore requires methods for determining the structure of cotranscriptional folding intermediates. Cotranscriptional RNA chemical probing methods accomplish this by systematically probing the structure of nascent RNA that is displayed from an RNA polymerase. Here, we describe a concise, high-resolution cotranscriptional RNA chemical probing procedure called variable length Transcription Elongation Complex RNA structure probing (TECprobe-VL). We demonstrate the accuracy and resolution of TECprobe-VL by replicating and extending previous analyses of ZTP and fluoride riboswitch folding and mapping the folding pathway of a ppGpp-sensing riboswitch. In each system, we show that TECprobe-VL identifies coordinated cotranscriptional folding events that mediate transcription antitermination. Our findings establish TECprobe-VL as an accessible method for mapping cotranscriptional RNA folding pathways.

Combustion Characteristics of Boron-Iron Powder with Micro-Zone Compounding

ABSTRACT Boron-iron composite powders with a D50 value of < 0.5 μm and iron contents of 5, 10, and 20 wt.% were prepared via a combination of electro-explosive and roll-grinding methods. High-resolution transmission electron microscopy and electron probe X-ray microanalysis revealed evidence of boron-ion micro-zone composites. Ignition tests using a 150-W laser in a pure oxygen atmosphere indicated that the boron-iron composite powder effectively reduced the ignition delay of boron (224.8 ms), with 5 wt.% boron-iron composite powder exhibiting a reduced ignition delay of 106.1 ms. Thermal analysis tests in a pure oxygen atmosphere indicated that iron not only promoted the exothermic reaction of boron but also significantly promoted its oxidation. A comparison of the oxygen bomb calorific values of the composite powder, the original boron powder, and simple mixed powder revealed that the simple mixed iron did not improve the combustion performance of boron, whereas the composite powder generally improved the boron calorific value (12967 J/g), with the 10 wt.% boron-iron composite powder exhibiting a boron calorific value of 21,189 J/g. The addition of iron improved the combustion behavior, stabilized the combustion flame, and improved the combustion quality. An elevated iron content resulted in an increased sensitivity of the powder to the ambient pressure, and an elevated pressure effectively increased the burning rate. The experimental results indicated that iron generally improved the ignition and combustion performance of boron with low and high iron contents, respectively.