Recently, high resolution micro-radiography became of great interest. Very thin scintillator layers of about 5–20 micrometres are used to achieve spatial resolution below one micrometre in application for low energy X-ray micro-radiography [1]. Such thin screens are mainly used in micro-XCT and nano-XCT systems (X-ray Computed Tomography) with either micro-focus X-ray tubes or with synchrotron sources [2]. This work deals with a high-resolution CCD camera together with different optical systems and different single crystal scintillators in application for low energy X-ray micro-radiography. The light distribution on the screen is re-imaged by an optical system (usually represented by classical microscope objective together with a field lens) to a high-resolution CCD or CMOS chip. A theoretical modelling and practical comparison of a camera set-up has been done to investigate the system resolution limits. Thin screens were prepared from YAG:Ce scintillator. The resolution is presented on test patterns. As recent optical systems are limited to the classical microscopy objectives only, we further investigate a possibility to develop a custom-made objective fully dedicated to X-ray imaging application.