Background: The Fujinon Company has recently developed an innovative technology of digital color enhancing integrated to the new processor EPX-4400. This technology allows the combination of spectral images generated from a color image decomposed according to specific wavelengths. The reconstruction of the image is made according to desired wavelengths giving the stage of reflectance of tissue by levels, from shallow to deep. The system presets 10 different options that can be freely changed. The combination of FICE with high resolution-magnification in the Fujinon EG 590 ZW colonoscope gives a potential advantage in the evaluation of the characteristics of the gastrointestinal mucosa and its pathological variations. Aim and Methods: This study includes 147 consecutive patients (83/56.5% males, 64/46.5% females) who had a colonoscopy performed. A comparison was made between direct visualization with FICE and recorded images and the final diagnosis made by biopsy. Results: We found endoscopic abnormalities in 95/64.6% patients that were correlated with biopsy. FICE technology improved our diagnosis without the need of vital stains mainly by recognizing small lesions. The accurate description of the microvascular pattern was made by wavelength set 0, 2, and 4. The density evaluated by light reflectance was made with filters 6 and 7. FICE set 4 and 6 significantly improved the definition of neoplastic margins in 5 cases. The villous appearance was well defined with set 4 and 6. Deep vascular pattern was present in set 0, 1 and 5. The method helped mainly in the definition of the best and most representative place to take the biopsy. Only in 2 cases the biopsy did not correlate with the endoscopic diagnosis. We had 12 cases that showed only minimal changes on endoscopy (increased nodularity or erosions), with a pathologic report of eosinophylic infiltrate or mild acute non-specific inflammation. The endoscopic observation time was not significantly increased. Conclusion: The application of this new technology combined with high resolution endoscopy has promising areas of development in the description of pathologic mucosal changes. This advanced technology allows an increased correlation between the images processed and the histological results. Further studies are needed to demonstrate all the possibilities of virtual chromoendoscopy.