High-resolution Digital Camera Research Articles

IMPROVEMENT OF CAPILLAROSCOPY METHOD FOR STUDYING HUMAN MICROCIRCULATION

The human circulatory system has historically captivated researchers in the field of medicine. Modern medicine, however, has moved beyond identifying a single crucial element, such as the heart or venous system, for maintaining blood flow. Instead, it emphasizes a more analytical approach, focusing on the interconnected functioning of all components within the system. The aim of this work is to analyze the existing methods of studying blood microcirculation and to improve the technology of capillaroscopy, technical means of raster microphotography to determine the physiological state and disorders of capillary circulation. To achieve this objective, we address the following tasks: analyzing existing non-invasive methods for studying the human vascular system; enhancing the optical capillaroscopy method through the utilization of modern high-resolution digital cameras, computer, and multimedia equipment, along with appropriate software for analyzing electronic images; developing a technological scheme and equipment design for digital microphotography of capillaries in the periungual region of the upper extremities; providing software solutions for registering and analyzing digital microphotographic images obtained through capillaroscopy; conducting experimental studies to explore the structure and properties of capillaries utilizing the developed technologies and equipment. Magnetic resonance imaging (MRI) enables the comprehensive evaluation of both anatomical and functional aspects of blood flow. Magnetic resonance angiography (MRA) capitalizes on the distinction between the signal emitted by moving tissue (blood) and that of surrounding stationary tissue, facilitating the acquisition of vascular images without the need for radiopaque contrast agents. Ultrasonography integrates Doppler and conventional ultrasound techniques, providing physicians with insights into blood vessel structure and blood flow dynamics. Traditional ultrasound employs sound waves that are imperceptible to the human ear and bounce off blood vessels, while Doppler ultrasound measures the velocity of sound wave reflection from moving elements. Ophthalmoscopy constitutes a fundamental component of standard ophthalmological examinations, serving as a pivotal tool for diagnosing eye conditions and evaluating the condition of blood vessels. Additionally, ophthalmoscopy aids in diagnosing autoimmune disorders. Capillaroscopy enables comprehensive assessments of both systemic and regional microcirculation disorders, facilitating the characterization of tissue metabolism. Dysfunction in capillary function contributes to circulatory impairments, leading to blood stasis, metabolic irregularities, and compromised immunity, thereby exacerbating existing conditions and predisposing individuals to new diseases. Optical computerized capillaroscopy offers a non-invasive means of visualizing, examining, and archiving capillary images, enabling clinicians to make informed assessments regarding blood microcirculation. Experimental validation of the optimized hardware and software capillaroscopy system was conducted using nail bed capillaries of a patient with type II diabetes mellitus undergoing insulin therapy for 10 years. This study underscores the importance of refining and enhancing optical capillaroscopy methodologies by leveraging high-resolution camera sensors and modern computational tools for image processing.

Different scenarios in sloshing flows near the critical filling depth

In the present paper, the sloshing flow in a cuboid tank forced to oscillate horizontally is investigated with both experimental and numerical approaches. The filling depth chosen is $h/L=0.35$ (with h the water depth and L the tank height), which is close to the critical depth. According to Tadjbakhsh & Keller (J. Fluid Mech., vol. 8, issue 3, 1960, pp. 442–451), as the depth passes through this critical value the response of the resonant sloshing dynamics changes from ‘hard spring’ to ‘soft spring’. The experimental tank has a thickness of $0.1L$ , reducing three-dimensional effects. High-resolution digital camera and capacitance wave probes are used for time recording of the surface elevation. By varying the oscillation period and the amplitude of the motion imposed on the tank, different scenarios are identified in terms of free-surface evolution. Periodic and quasi-periodic regimes are found in most of the frequencies analysed but, among these, sub-harmonic regimes are also identified. Chaotic energetic regimes are found with motions of greater amplitude. Typical tools of dynamical systems, such as Fourier spectra and phase maps, are used for the regime identification, while the Hilbert–Huang transform is used for further insight into doubling-frequency and tripling-period bifurcations. For the numerical investigation, an advanced and well-established smoothed particle hydrodynamics method is used to aid the understanding of the physical phenomena involved and to extend the range of frequencies investigated experimentally.

Non-invasive calibration of a Stewart platform by photogrammetry

AbstractAccurate calibration of a Stewart platform is important for their precise and efficient operation. However, the calibration of these platforms using forward kinematics is a challenge for researchers because forward kinematics normally generates multiple feasible and unfeasible solutions for any pose of the moving platform. The complex kinematic relations among the six actuator paths connecting the fixed base to the moving platform further compound the difficulty in establishing a straightforward and efficient calibration method. The authors developed a new forward kinematics-based calibration method using Denavit-Hartenberg (DH) convention and used the Stewart platform “Tiger 66.1” developed in their lab for experimenting with the photogrammetry-based calibration strategies described in this paper. This system became operational upon completion of construction, marking its inaugural use. The authors used their calibration model for estimating the errors in the system and adopted three compensation options or strategies as per least-square method to improve the accuracy of the system. These strategies leveraged a high-resolution digital camera and off-the-shelf software to capture the poses of the moving platform’s center. This process is non-invasive and does not need any additional equipment to be attached to the hexapod or any alteration of the hexapod hardware. This photogrammetry-based calibration process involves multiple high-resolution images from different angles to measure the position and orientation of the platform center in the three-dimensional space. The target poses and actual poses are then compared, and the error compensations are estimated using the least-square methods to calculate the predicted poses. Results from each of the three compensation approaches demonstrated noticeable enhancements in platform pose accuracies, suggesting room for further improvements. Given that “Tiger 66.1” is based on the general Stewart platform structure, the proposed calibration method holds promise for extension to machines operating on similar principles where non-invasive calibration is desirable. This study contributes to advancing the field of Stewart platform calibration, paving the way for more precise and efficient applications in various domains.

Detection of Sub-surface Delamination and Moisture Penetration in Unlined Rock Tunnels Using Passive Thermography and Tapping

An unlined rock-blast tunnel constructed by the drill and blast method is susceptible to frequent deterioration, including sagging beds, water ingress and loose rock masses. Thus, assessing potential damages above the roadway that may impact the safety of tunnel users is of utmost importance. While tapping, a conventional nondestructive method, is typically used to predict sub-surface delamination, water ingress can be identified with the naked eye. In this research, we utilized a modern-technology approach called passive thermography, together with a high-resolution digital camera and tapping. The study encountered two primary challenges; namely, the absence of direct solar radiation during the inspection and the groove-exposed surface of the unlined tunnel. The results demonstrated that passive thermography was able to detect delaminated areas and water infiltration on the tunnel's walls and ceiling, even without direct sunlight. Large delaminated areas can be detected with just a 2°C change in atmospheric temperature twelve hours before testing. Additionally, the results of image post-processing significantly contributed to enhancing the results of passive thermography. The thermal image was processed into a grayscale image prior to HE processing, which enhances contrast by over 50%. The combination of tapping, digital camera and passive thermography was proven to be effective in periodically inspecting unlined rock tunnels, while significantly reducing time and cost. KEYWORDS: Tunnel safety, Passive thermography, Old rock-cut tunnel, Hammer sounding, Non-destructive evaluation, Image post-processing.

Método DIC aplicado na calibração de um modelo MEF de interface coesiva rocha-argamassa

Abstract In civil engineering, the stability of many structures depends on the physical properties of the contact between the concrete seated on the rocky foundation surface. Usually, they are critical structures of great importance to society, such as: bridges, tunnels and dams. Thus, the shear behavior of rock-concrete joints is a key factor of the structural stability. The method of cohesive zones (CZM) allows to simulate the beginning of the formation of a crack and its propagation, without knowing the crack location or when it will start. Thus, in this work, a numerical model was developed capable of simulating the failure due to interfacial delamination of the contact between a structure formed by mortar seated on a rocky granite leaning surface. The calibration of the numerical model was performed using the experimental test results of simple compression on a block of rock in contact with the mortar by an inclined interface. The test was monitored using a high-resolution digital camera. The data were processed using the digital image correlation method (DIC) to obtain displacement data of the laboratory test. The DIC results were used to calibrate the nonlinear numerical model of the test using the bilinear method of cohesive zone in the rock-mortar interfacial contact elements. By a parametric analysis varying the maximum shear stress parameter of the contact cohesive elements, the bilinear law was adjusted (Maximum tensile stress and critical displacement and damage rate). Indirectly, the rate of release of energy of critical deformation from the rupture of the contact was also estimated.

Effects of Hair Essence Containing Vitamin E, Vitamin A, and Phytantriol on Hair Quality

Purpose: This study examined the effect of a hair essence containing α-tocopheryl acetate, retinyl palmitate, and phytantriol (TRP-hair essence) on damaged hair and hair quality characteristics such as hair volume and gloss.Methods: The TRP-hair essence containing 0.01% (w/w) each of α-tocopheryl acetate (vitamin E), retinyl palmitate (vitamin A), and phytantriol was prepared. The effect of the TRP-hair essence on damaged hair was analyzed using a scanning electron microscope; its impact on hair volume and gloss was confirmed using a high-resolution digital camera. Furthermore, a sensory evaluation was used to determine satisfaction after using the TRP-hair essence.Results: The cuticle of the hair that had been damaged because of chemical treatment improved by 19.8% (<i>p</i><0.05) following only a single round of the TRP-hair essence treatment. Hair volume and gloss significantly increased immediately and 24 hours after using the TRP-hair essence (<i>p</i><0.05). There was substantial satisfaction in terms of the sensory evaluation of directly using the TRP-hair essence on human hair.Conclusion: Using the TRP-hair essence effectively recovered the damaged hair and improved its quality characteristics such as hair volume and gloss, resulting in high satisfaction with use.

Trends in development of agricultural aerial photography technology

Aerial photography is becoming an integral part of remote sensing in digital agriculture. The first aerial photographs were taken in the mid-19th century. (Research purpose) The paper aims to retrospectively analyze the evolution of aerial photography equipment for capturing agricultural lands, beginning with the creation of the first aerial photograph up to the present day. (Materials and methods) A historical-analytical approach was employed to examine the existing literature. Within this study, the development of agricultural aerial photography equipment was categorized into four distinct time periods: 1885-1908, 1909-1945, 1946-1979, and from 1980 to the present day. (Results and discussion) In the initial phase of experimental aerial photographic equipment development, significant advancements were achieved, encompassing the emergence of the first photograph, the creation of portable cameras and their adaptation for use with hot air balloons and kites, rockets, and birds. Technological growth in the first half of the 20th century contributed to elevating aerial photography to a versatile tool applied for a wide range of intelligence operations, including agricultural tasks. The evolution of space technologies in the second half of the 20th century resulted in the rapid development of both aerial photography equipment and their carriers. This progress facilitated the use of color aerial photography for the examination of the Earth's surface. The advancements of digital technologies at the end of the 20th century and the beginning of the 21st century facilitated the use of high-resolution digital aerial cameras mounted on various carrier platforms, ranging from unmanned aircraft to artificial Earth satellites. (Conclusions) A retrospective analysis reveals that the development and creation of equipment for aerial photography of agricultural lands unfolded in a sporadic fashion. This progression was closely intertwined with global political, social, and economic situation, as well as the state of technological advancement in related areas. Over the coming decade, the sustained application of aerial photography in agriculture is poised to enhance the efficiency of unmanned aircraft, reduce the production costs associated with aerial photography, and facilitate the widespread adoption of digital remote sensing technology within the agricultural sector.

Face Recognition and Eye Blink Attendance Management System

This research explores the implementation and advantages of utilizing facial recognition technology for automated student attendance management in educational environments. The study outlines a comprehensive system that employs a high-resolution digital camera to detect and recognize students' faces, comparing them with stored images in a database. Successful matches result in real-time attendance logging, enhancing record-keeping and future analysis. The system also accommodates the inclusion of new images when no match is found. By embracing deep learning advancements, the technology offers an efficient alternative to conventional roll-call procedures, enabling automatic head counting in classrooms. The system integrates various face recognition algorithms, leveraging attributes such as shape, color, Local Binary Pattern (LBP), wavelet transforms, and auto-correlation. While challenges like varying lighting conditions, facial expressions, and face orientation are acknowledged, this research underscores the potential of technology to revolutionize attendance management and student engagement in education. Key Words: facial recognition technology, student attendance, automated system, deep learning, real-time logging, attendance management, classroom headcount, face recognition algorithms, record-keeping.

Quantitative Trait Locus (QTL) Mapping for Common Wheat Plant Heights Based on Unmanned Aerial Vehicle Images

The application of unmanned aerial vehicle (UAV) remote sensing technology for high-throughput acquisition of phenotypic values of field plant height is of great significance in plant height monitoring of wheat varieties (lines). Based on the UAV, mounted with high-resolution digital cameras, a low-altitude remote sensing platform was built to obtain images of the Berkut/Worrakatta recombinant inbred lines (RIL) wheat population (297 lines). The optimal digital surface model (DSM) for plant height extraction was constructed by combining the actual wheat plant heights measured in the field. Using a wheat 50K SNP chip, quantitative trait locus (QTL) analysis was performed for field plant height of the RIL population using UAV plant height data to detect the locus related to plant height. We verified the effectiveness of QTL analysis using the UAV plant height data. The results of the DSM plant height extraction method showed that the plant height extraction models during the entire growth period had a highly significant correlation (p < 0.001); the measured plant height correlated with the predicted plant height, with prediction model accuracy R2 = 0.7527 and verification model accuracy R2 = 0.8214. QTL analysis was conducted on the manually and UAV-measured plant height traits. For the manually measured plant height phenotypes, one locus related to plant height was detected on wheat chromosome 6A and explained 13.12% of phenotypic variation. For the UAV-measured phenotypes, one locus related to plant height was also detected on wheat chromosome 6A and explained 9.62% of phenotypic variation. The QPH.xjau-6A locus on chromosome 6A, which featured in the results of the two measurement methods, is a stable locus, indicating that the mapping results obtained using the actual plant height values were in good agreement with those obtained using the UAV extracted values. Three candidate genes related to plant height were screened: they encode protein kinase, NAC domain protein, and cytochrome P450, respectively. Therefore, this study provided reference information for monitoring plant phenotype and growth of wheat and also for the extraction of plant height for wheat breeding in the future.

Digital Holographic Interferometry for Wholefield NDT Applications

Holographic interferometry is a non-contact whole field optical technique used as a powerful NDT tool. This technique is highly sensitive and maps the object surface deformation as holographic fringe pattern. Conventional holography technique has practical difficulties in recording and reconstructing holograms. With the development in imaging sensor technology and fast algorithms in image processing, digital holography has been established. Digital holography uses high-resolution digital camera for recording holograms and does reconstruction of holograms numerically using a computer. This has enhanced the scope and application of holography for NDT many fold. In this paper application of digital holography to non-destructive testing is presented. The basic principle of digital holography and digital holographic interferometry in obtaining double exposure hologram for NDT is described. The software developed in house for numerical reconstruction of holograms is also explained. The advantages of digital holography over conventional holography in NDT applications are highlighted. Extensive experiments were carried out to prove the detectability of defects in both metallic and non-metallic materials and the results are presented. It is shown that digital holography is able to identify defects in a wide range of materials with great ease because of its high sensitivity and whole field nature.

Lip color measurement: A new hyperspectral imaging device.

The lips are of significant interest among the facial features for self-perception and social interactions. Such perceptions could rely in part upon the contrast in color between the lips and the surrounding skin. In contrast to skin color, lip color ethnic diversity measurement provides a challenge and is less documented in the literature, most likely due primarily to challenges in measurement based upon the lip characteristics of size and contour. Accurate measurement and reproduction of lip color are essential to build strong cross-sectional knowledge about lip color. This preliminary experiment of a large-scale project on lip and skin color was designed to assess accuracy in lip color measurement and recommend instruments and protocols for good measurement practice. The study involved 19 healthy French Caucasian women. Their inferior and superior natural lip color was measured twice with three devices in order to extract the standard colorimetric parameters of lightness (L*), chroma (C*), and hue (h): the VISIA-CR® (high-resolution digital camera, Canfield Scientific Inc.); the spectrophotometer VS3200 (45°:0° spectrophotometer, MetaVue™ VS3200, X-Rite Inc.); and the SpectraFace® (new hyperspectral imaging system, Newtone Technologies). The intercomparison and color distribution between the different instruments and the measurement repeatability of each one were assessed. Our results showed a reliable and equivalent repeatability for the three tested instruments. Moreover, the three tools led to the same conclusion regarding lip color heterogeneity (lighter, redder, less yellow and more saturated inferior lip versus superior lip). Agreement between the SpectraFace® and the spectrophotometer measurements was higher, and the SpectraFace® allowed to identify three separated lip color typologies. In conclusion, the combination of imaging technology and hyperspectral measurement makes the SpectraFace® a useful tool to investigate lip color characterization and establish a large-scale cross-sectional knowledge study about lip color.

Use of unmanned ground vehicle systems in urbanized zones: A study of vector Mosquito surveillance in Kaohsiung.

Dengue fever is a vector-borne disease that has become a serious global public health problem over the past decade. An essential aspect of controlling and preventing mosquito-borne diseases is reduction of mosquito density. Through the process of urbanization, sewers (ditches) have become easy breeding sources of vector mosquitoes. In this study, we, for the first time, used unmanned ground vehicle systems (UGVs) to enter ditches in urban areas to observe vector mosquito ecology. We found traces of vector mosquitoes in ~20.7% of inspected ditches, suggesting that these constitute viable breeding sources of vector mosquitoes in urban areas. We also analyzed the average gravitrap catch of five administrative districts in Kaohsiung city from May to August 2018. The gravitrap indices of Nanzi and Fengshan districts were above the expected average (3.26), indicating that the vector mosquitoes density in these areas is high. Using the UGVs to detect positive ditches within the five districts followed by insecticide application generally yielded good control results. Further improving the high-resolution digital camera and spraying system of the UGVs may be able to effectively and instantly monitor vector mosquitoes and implement spraying controls. This approach may be suitable to solve the complex and difficult task of detecting mosquito breeding sources in urban ditches.

Measurement of Cracks in Concrete Bridges by Using Unmanned Aerial Vehicles and Image Registration

Crack development is a clear indicator of the durability of concrete bridges. Traditional bridge inspections that rely inspectors to climb on bridges with lift cars are unsafe for inspectors and also time- and labor-consuming. Therefore, this research proposes a solution that applies unmanned aerial vehicles (UAV) and high-resolution digital cameras to measure concrete bridge cracks. An experiment was conducted on an Ai-He concrete bridge located in Yangmei District, Taoyuan City, Taiwan. Two types of images were taken. Close-up images observed cracks more clearly, and long-range images covered the ground control points. We registered these two types of images to establish the absolute coordinate system with ground control points and tie points through block triangulation. This research examines three approaches of generating tie points: (1) manually select tie points with features on the bridge such as nails and dots, (2) randomly input tie points generated from Scale-Invariant Feature Transform (SIFT), and (3) randomly input tie points generated from SIFT as the initial tie points and perform automatic tie generation with the ERDAS Leica Photogrammetry Suite (LPS) image matching module (automatic tie generation). Afterwards, close-up images were processed into orthorectified images with 0.1 mm pixel size for crack size measurements. Crack sizes were determined by a manual measurement approach and an inflection point approach for comparison. This research established a workflow for UAV bridge inspection that locates and measures cracks in concrete bridges, which consequently provides a safe and cost-efficient concrete bridge crack monitoring solution with acceptable accuracies.

Influence of Soluble Salt NaCl on Cracking Characteristics and Mechanism of Loess

Under the conditions of drought, cracks are likely to appear in loess due to shrinkage, which leads to salt precipitation and accumulation on the surface of loess. Therefore, salinized lands are created in loess areas. Deep study into the influence of soluble salt content on the cracking characteristics and mechanism of loess is of great significance to engineering constructions, geological problems, and disaster prevention for salinized lands in loess regions. In this paper, free desiccation experiments were carried out on the loess samples with different NaCl concentrations (a soluble salt). A high-resolution digital camera was used to obtain the sequence images of loess during the drying process. With the advantage of digital image correlation (DIC) technology and the non-contact full-field strain measurement method, the local displacement and strain on the surface of loess samples were calculated. The microstructure and main elements distribution of loess samples were obtained by scanning electron microscopy (SEM) and energy-dispersive spectrum (EDS) methods. Finally, the influence of NaCl concentrations on cracking characteristics and mechanism of loess was analyzed. The results show that, with the increase in NaCl concentration, the evaporation rate of loess samples decreased and the residual water content increased. The NaCl content can prevent the development of desiccation cracks in loess.

Differential Expression of Na/K-ATPase in the Human Saccule of Patients With and Without Otologic Disease.

Na + , K + -ATPase (Na/K-ATPase) α1 subunit expression in the saccule of patients diagnosed with otologic disease is different compared with normal controls. We have recently characterized changes in the expression of Na/K-ATPase α1 subunit in the normal and pathological cochlea; however, no studies have determined the distribution Na/K-ATPase α1 subunit in the human saccule. The present study uses archival temporal bones to study the expression Na/K-ATPase α1 subunit in the human saccule. Archival celloidin formalin fixed 20-micron thick sections of the vestibule from patients diagnosed with Menière's disease (n = 5), otosclerosis (n = 5), sensorineural hearing loss, and normal hearing and balance (n = 5) were analyzed. Sections containing the saccular macula were immunoreacted with mouse monoclonal antibodies against Na/K-ATPase α1 subunit. Micrographs were acquired using a high-resolution digital camera coupled to a light inverted microscope. In the normal human saccule vestibular sensory epithelium, Na/K-ATPase α1 immunoreactivity (IR) was present in nerve fibers and calyces that surround type I vestibular hair cells and nerve terminals. The transition epithelium cells were also Na/K-ATPase α1 immunoreactive. Comparison between normal and pathological specimens showed that there was a significant reduction of Na/K-ATPase α1 IR in the saccule vestibular sensory epithelium from patients with Menière's disease, otosclerosis, and sensorineural hearing loss. The decrease of Na/K-ATPase-IR α1 in the saccule vestibular sensory epithelium from patients with otopathologies suggests its critical role in inner ear homeostasis and pathology.

The Impact of Simulated Bruxism Forces and Surface Aging Treatments on Two Dental Nano-Biocomposites-A Radiographic and Tomographic Analysis.

Background and Objectives: Nowadays, indication of composite materials for various clinical situations has increased significantly. However, in the oral environment, these biomaterials are subjected (abnormal occlusal forces, external bleaching, consumption of carbonated beverages, etc.) to changes in their functional and mechanical behavior when indicated primarily for patients with masticatory habits. The study aimed to recreate in our lab one of the most common situations nowadays-in-office activity of a young patient suffering from specific parafunctional occlusal stress (bruxism) who consumes acidic beverages and is using at-home dental bleaching. Materials and Methods: Sixty standardized class II cavities were restored with two nanohybrid biocomposite materials (Filtek Z550, 3M ESPE, and Evetric, Ivoclar Vivadent); the restored teeth were immersed in sports drinks and carbonated beverages and exposed to an at-home teeth bleaching agent. The samples were subjected to parafunctional mechanical loads using a dual-axis chewing simulator. A grading evaluation system was conducted to assess the defects of the restorations using different examination devices: a CBCT, a high-resolution digital camera, and periapical X-rays. Results: Before mechanical loading, the CBCT analysis revealed substantially fewer interfacial defects between the two resin-based composites (p > 0.05), whereas, after bruxism forces simulation, significantly more defects were identified (p < 0.05). Qualitative examination of the restorations showed more occlusal defects for the Evetric than the other nanohybrid composite. Conclusions: There were different behaviors observed regarding the studied nanocomposites when simulation of parafunctional masticatory forces was associated with aging treatments.

Method to obtain 3D point clouds of tunnels using smartphone LiDAR and comparison to photogrammetry

Remote sensing methods, for example, photogrammetry and laser scanning can be employed to scan rock masses by digitizing underground spaces or slopes. To reconstruct a 3D model by photogrammetry, plenty of photos should be captured. This process is time-consuming and can be dangerous when the rock mass is unstable. Therefore, rapid capturing methods are needed to reduce the acquisition time. This can be achieved with LiDAR scanners that capture a 3D point cloud with a high-speed pulsed laser beam. However, the cost of laser scanners is high, which limits their usability. Apple’s iPhone 12 Pro Max smartphone is equipped with a LiDAR sensor and is much cheaper than conventional laser scanners. Several mobile applications for 3D models using the smartphone Lidar have been developed, making the scanning process easy. Therefore, smartphones can be used for rapid scanning of unstable surfaces of tunnels and slopes. In this study, 3D point clouds (3DPC) of a tunnel wall of the Underground Research Laboratory of Aalto University (URLA) are obtained by a high-resolution digital camera and smartphone LiDAR. The models are analyzed and compared. The results show the quality of the smartphone LiDAR sensor is adequate for generating 3D models of underground spaces.

Role of Prostaglandins in Nitric Oxide-Induced Glial Cell-Mediated Vasodilation in Rat Retina.

We previously identified that NO derived from neuronal cells acts on glial cells and causes vasodilation in the healthy rat retina via the release of epoxyeicosatrienoic acids (EETs) and prostaglandins (PGs) by activation of the arachidonic acid cascade. However, it is not clear which PG types are involved in these responses. The aim of the present study was to identify prostanoid receptors involved in glial cell-derived vasodilation induced by NO in rat retina. Male Wistar rats were used to examine the effects of intravitreal pretreatment with indomethacin, a cyclooxygenase inhibitor; PF-04418948, a prostanoid EP2 receptor antagonist; and CAY10441, a prostanoid IP receptor antagonist, on the changes in the retinal arteriolar diameter induced by intravitreal administration of NOR3, an NO donor. Retinal arteriolar diameters were measured using ocular fundus images captured with a high-resolution digital camera in vivo. The increase in the retinal arteriolar diameter induced by intravitreal injection of NOR3 was significantly suppressed by intravitreal pretreatment with indomethacin and PF-04418948, but not by CAY10441. The dose of PF-04418948 and CAY10441 injected intravitreally in the present study significantly reduced the increase in the retinal arteriolar diameter induced by prostaglandin E2 (PGE2) and prostaglandin I2 (PGI2), respectively. These results suggest that activation of the arachidonic acid cascade and subsequent stimulation of prostanoid EP2 receptors are involved in rat retinal vasodilatory responses evoked by NO-induced glial cell stimulation. Therefore, glial cell-derived PGE2, similar to EETs, may play an important role in retinal vasodilatory mechanisms.

Effects of diethyl ether addition on sooting transition in iso-octane counterflow diffusion flames

This study studied the effects of the addition of diethyl ether (DEE) in the fuel stream on sooting transition process in iso-octane counterflow diffusion flames with optical and chemical diagnostics. Compared with the baseline iso-octane flame, two fuel/oxygen/nitrogen flames were studied with a 20 % DEE/iso-octane and a 40 % DEE/iso-octane mixture as the fuel, while holding constant fuel mole fraction. Optical diagnosis was used for the investigation of the effect of DEE addition on macroscopic sooting transition point with a high-resolution digital camera based on image processing and transition point extraction methods. Online gas chromatograph (GC) was used for all flames to investigate the flame structure and measure quantitative mole fraction profiles of stable and reactive species formed in the sooting transition process, especially the changes caused by partial substitution of iso-octane by DEE. Meanwhile, the experimental results were compared with predictions from a model suitable for both iso-octane and DEE. Sooting transition point was delayed with the increase of DEE addition. Although the overall flame chemical structure did not change significantly with the addition of DEE, it was found that some primary decomposition products and precursors had more significant effects. In most cases, experimental trends could be predicted and explained well by the model. It was noted that the dilution of carbon flow caused by partial substitution was the main reason for the inhibition of soot formation and the delay of sooting transition point. Secondly, on the chemical effect, DEE addition could produce a large amount of C2H2 which could contribute to the production of C3H3 and the formation of large PAHs.

Sooting transition chemistry for iso-octane/n-butanol counterflow diffusion flames

This work focused on effects of n-butanol addition on sooting transition process in iso-octane counterflow diffusion flames using optical and chemical diagnostics. Three iso-octane flames were studied with pure iso-octane, a 20% n-butanol addition mixture and a 40% n-butanol addition mixture as fuels. Optical diagnostics was carried out to determine effects of n-butanol addition on the sooting transition process using a high-resolution digital camera based on image processing and transition point extraction methods. Online gas chromatograph was used to investigate the flame chemistry in the sooting transition process. The results showed that the sooting transition point was delayed with the increase of n-butanol addition, which indicated that the addition of n-butanol could suppress the soot formation. To reveal the mechanism for effects of n-butanol on sooting transition process, kinetic simulations were further performed. The experimental results of flame chemistry were compared with the predictions from two mechanisms for iso-octane and n-butanol mixture. Both mechanisms were found to be capable of well capturing the main variations of most species in flames. After n-butanol addition, the changes of fuel-specific primary decomposition products were more obvious. It was noted that the decreased concentration of carbon which was available for benzene formation was the main reason for the delay of sooting transition point with n-butanol additions. Moreover, the inhibition of PAHs growth from n-butanol addition also could contribute to this kind of delayed effects.