High-resolution Cross Sections Research Articles

Development of a cyclic ion mobility spectrometry-mass spectrometry-based collision cross-section database of permethylated human milk oligosaccharides.

Human milk oligosaccharides (HMOs) are an important class of biomolecules responsible for the healthy development of the brain-gut axis of infants. Unfortunately, their accurate characterization is largely precluded due to a variety of reasons - there are over 200 possible HMO structures whereas only 10s of these are available as authentic analytical standards. Furthermore, their isomeric heterogeneity stemming from their many possible glycosidic linkage positions and corresponding α/β anomericities further complicates their analyses. While liquid chromatography coupled to tandem mass spectrometry remains the gold standard for HMO analyses, it often times cannot resolve all possible isomeric species and thus warrants the development of other orthogonal approaches. High-resolution ion mobility spectrometry coupled to mass spectrometry has emerged as a rapid alternative to condensed-phase separations but largely has remained limited to qualitative information related to the resolution of isomers. In this work, we have assessed the use of permethylation to improve both the resolution and sensitivity of HMO analyses with cyclic ion mobility separations coupled with mass spectrometry. In addition to this, we have developed the first-ever high-resolution collision cross-section database for permethylated HMOs using our previously established calibration protocol. We envision that this internal reference database generated from high-resolution cyclic ion mobility spectrometry-mass spectrometry will greatly aid in the accurate characterization of HMOs and provide a valuable, orthogonal, approach to existing liquid chromatography-tandem mass spectrometry-based methods.

High-Resolution Rovibronic Cross Sections of the MgH+ Molecular Cation.

The high-resolution rovibronic line lists of the MgH+ molecular cation are presented in our work. The potential energy curves are calculated by the method of multireference configuration interaction and Davidson correction (MRCI+Q) with the spin-orbit coupling (SOC) effect. Spectroscopy constants are fitted and the results are in good agreement with experiments, ensuring the accuracy of the electronic structure. On account of potential energy curves and transition dipole moments, the Franck-Condon factors and Einstein coefficients of transition are obtained. These calculations are used to obtain an accurate partition functions and line lists for molecules. Using the partition functions and line lists, the absorption cross-sections under different temperatures and pressures were simulated. Our work could provide some theoretical insights into solar and cold planet spectrum.

Enhancement of the NORAD-Atomic-Data Database in Plasma

We report recent enhancements to the online atomic database at the Ohio State University, NORAD-Atomic-Data, that provide various parameters for radiative and collisional atomic processes dominant in astrophysical plasma. NORAD stands for Nahar Osu RADiative. The database belongs to the data sources, especially for the latest works, of the international collaborations of the Opacity Project and the Iron Project. The contents of the database are calculated values for energies, oscillator strengths, radiative decay rates, lifetimes, cross-sections for photoionization, electron-ion recombination cross-sections, and recombination rate coefficients. We have recently expanded NORAD-Atomic-Data with several enhancements over those reported earlier. They are as follows: (i) We continue to add energy levels, transition parameters, cross-sections, and recombination rates for atoms and ions with their publications. (ii) Recently added radiative atomic data contain a significant amount of transition data for photo-absorption spectral features corresponding to the X-ray resonance fluorescence effect, showing prominent wavelength regions of bio-signature elements, such as phosphorus ions, and emission bumps of heavy elements, such as of lanthanides, which may be created in a kilonova event. We are including (iii) collisional data for electron-impact-excitation, (iv) experimental data for energies and oscillator strengths for line formation, (v) experimental cross-sections for photoionization that can be applied for benchmarking and other applications, and (vi) the introduction of a web-based interactive feature to calculate spectral line ratios at various plasma temperature and density diagnostics, starting with our recently published data for P II. We presented a summary description of theoretical backgrounds for the computed data in the earlier paper. With the introduction of experimental results in the new version of NORAD, we present a summary description of measurement of high-resolution photoionization cross-sections at an Advanced Light Source of LBNL synchrotron set-up and briefly discuss other set-ups. These additions should make NORAD-Atomic-Data more versatile for various applications. For brevity, we provide information on the extensions and avoid repetition of data description of the original paper.

High-resolution cross section measurements for neutron interactions on $$^{89}$$Y with incident neutron energies up to 95 keV

High-resolution cross section measurements for neutron interactions on $$^{89}$$Y with incident neutron energies up to 95 keV

Arabidopsis Root Microbiome Microfluidic (ARMM) Device for Imaging Bacterial Colonization and Morphogenesis of Arabidopsis Roots.

Imaging the spatiotemporal dynamics of host-microbiota interactions is of particular interest for augmenting our understanding of these complex systems. This is especially true of plant-microbe interactions happening around, on, and inside plant roots where relatively little is understood about the dynamics of these systems. Over the past decade, a number of microfluidic devices have been developed to grow plants hydroponically in gnotobiotic conditions and image morphogenesis of the root and/or dynamics with fluorescently labeled bacteria from the plant root microbiome. Here we describe the construction and use of our Arabidopsis Root Microbiome Microfluidic (ARMM) device for imaging fluorescent protein expressing bacteria and their colonization of Arabidopsis roots. In contrast to other plant root imaging devices, we designed this device to have a larger chamber for observing Arabidopsis root elongation and plant-microbe interactions with older seedlings (between 1.5 and 4weeks after germination) and a 200μm chamber depth to specifically maintain thin Arabidopsis roots within the focal distance of the confocal microscope. Our device incorporates a new approach to growing Arabidopsis seedlings in screw-top tube caps for simplified germination and transfer to the device. We present representative images from the ARMM device including high resolution cross section images of bacterial colonization at the root surface.

Control of basin-scale accommodation zones on syn-rift Aptian lacustrine carbonate reservoirs. Implications for reservoir quality estimates of Brazilian pre-salt

This paper presents the tectono-sedimentary time and space evolution of the Sururu oil field (Brazilian Pre-Salt). The field is located at the well-known Santos External High (SEH), north of the Tupi oil field—the pioneer accumulation of the largest and most prolific hydrocarbon trend of the 21st century. In the central portion of the field, the regional NE-trending faults system that prevails in the Santos Basin bends to NNW and becomes diffused into several low-displacement normal faults. These faults control a series of segmented structural highs, with some holding significant amounts of oil within lower permeability mudstones. These are the reservoir rocks, controlled by an NW horst, segmented in three domains by 290° Az and 245° Az trending relay ramp systems. The structural domains control discontinuous depocenters that evolved during the late syn-rift phase (Mid Aptian, c. 117 Ma). In this work, the aim is to show how the evolution through time of these segmented domains influenced variations of sedimentary facies, affecting vertical connectivity of the oil within the production zones. The work was based on interpreting 3D multi-azimuthal high-resolution seismic data, eight wells, and cross-section restoration techniques. We interpret multi-stage fault growth along the NW structural trend as a mechanism for distributing reservoir rocks, promoting localized syn-rift footwall uplift of isolated normal faults. The intervening domains between interacting faults induce the nucleation of accommodation zones, which in turn, controls the distribution of sediment catchment areas. Facies quality degradation along syn-rift structural highs impacts the distribution of preferential fluid flow. Therefore, the association of structural elements described in this study demonstrates that shallow water facies were controlled by uplift at the footwall of low displacement normal faults, inducing local lake-level retraction. Such evolution is strongly influenced by displacement transfer along local transfer zones dissecting the structural highs, which in turn controls local structural highs and lows, hence the distribution of high- and low-quality reservoir rocks during later phases of rifting.

Gas-assisted femtosecond pulsed laser machining: A high-throughput alternative to focused ion beam for creating large, high-resolution cross sections.

Cross sectioning is a critical sample preparation technique used in a wide range of applications, that enables investigation of buried layers and subsurface features or defects. State-of-the-art cross-sectioning methods, each have their own pros and cons, but generally suffer from a tradeoff between throughput and accuracy. Mechanical methods are fast but lack accuracy. On the other hand, ion-based methods, such as focused ion beam (FIB), offer high resolutions but are slow. Lasers, which can potentially improve this tradeoff, face multiple challenges that include creation of heat affected zones (HAZs), undesirably large spot size as well as material redeposition. In this work, we utilized, for the first time, a femtosecond pulsed laser, which has been shown to cause minimal to zero HAZ, for rapid creation of large cross sections that are comparable with FIB cross sections in quality. The laser was integrated with a targeted CO2 gas delivery system for redeposition control and beam tail curtailing, and a hard mask for top surface protection and further shrinkage of the effective spot size. The performance of the proposed system is showcased through real world examples that compare the throughput and quality resulted from the laser and FIB cross sectioning techniques.

Electronic State Spectroscopy of Nitromethane and Nitroethane.

High-resolution photoabsorption cross-sections in the 3.7-10.8 eV energy range are reinvestigated for nitromethane (CH3NO2), while for nitroethane (C2H5NO2), they are reported for the first time. New absorption features are observed for both molecules which have been assigned to vibronic excitations of valence, Rydberg, and mixed valence-Rydberg characters. In comparison with nitromethane, nitroethane shows mainly broad absorption bands with diffuse structures, which can be interpreted as a result of the side-chain effect contributing to an increased number of internal degrees of freedom. New theoretical quantum chemical calculations performed at the time-dependent density functional theory (TD-DFT) level were used to qualitatively help interpret the recorded photoabsorption spectra. From the photoabsorption cross-sections, photolysis lifetimes in the terrestrial atmosphere have been obtained for both compounds. Relevant internal conversion from Rydberg to valence character is noted for both molecules, while the nuclear dynamics of CH3NO2 and C2H5NO2 along the C-N reaction coordinate have been evaluated through potential energy curves at the TD-DFT level of theory, showing that the pre-dissociative character is more prevalent in nitromethane than in nitroethane.

Pore Microstructure and Multifractal Characterization of Lacustrine Oil-Prone Shale Using High-Resolution SEM: A Case Sample from Natural Qingshankou Shale

Pore structure is one of the important parameters for evaluating reservoirs, critical in controlling the storage capacity and transportation properties of hydrocarbons. The conventional pore characterization method cannot fully reflect the pore network morphology. The edge-threshold automatic processing method is applied to extract and quantify pore structures in shale scanning electron microscope (SEM) images. In this manuscript, a natural lacustrine oil-prone shale in the Qingshankou Formation of Songliao Basin is used as the research object. Based on FE-SEM, a high-resolution cross-section of shale was obtained to analyze the microstructure of pores and characterize the heterogeneity of pores by multifractal theory. The stringent representative elementary area (REA) of the SEM cross-section was determined to be 35 × 35. Four pore types were found and analyzed in the stringent REA: organic pores, organic cracks, inorganic pores, inorganic cracks. The results showed that inorganic pores and cracks were the main pore types and accounted for 87.8% of the total pore area, and organic cracks were of the least importance in the Qingshankou shale. Inorganic pores were characterized as the simplest pore morphologies, with the largest average MinFeret diameter, and the least heterogeneity. Moreover, the inorganic cracks had a long extension distance and stronger homogeneity, which could effectively connect the inorganic pores. Organic pores were found to be the most complex for pore structure, with the least average MinFeret diameter, but the largest heterogeneity. In addition, the extension distance of the organic cracks was short and could not effectively connect the organic pore. We concluded that inorganic pores and cracks are a key factor in the storage and seepage capacity of the Qingshankou shale. Organic pores and cracks provide limited storage space.

A laser-absorption sensor for in situ detection of biofuel blend vapor in engine intakes

A novel, mid-infrared laser-absorption sensor was developed for measurements of biofuel blend (E85) vapor in the intake runner of a single-cylinder internal combustion engine. The sensor used two mid-infrared interband cascade lasers to target a region of strong E85 absorbance near 3.35 µm. The lasers were co-aligned and fiber-coupled into the engine, and a two-color, online-offline differential absorption strategy was used to provide sensitive measurements of E85 vapor mole fraction and reject interference due to beam-steering and fuel droplet spray. An intensity-modulation spectroscopy (IMS) sensing scheme was employed to reject low-frequency additive noise and allow for frequency-domain multiplexing of the two lasers. High-resolution E85 absorption cross-section measurements were conducted in a static cell at a range of intake-relevant conditions. Validation experiments were performed in a laboratory setting to demonstrate sensor accuracy and noise rejection capability. The sensor was deployed on a single-cylinder development engine during dynamometer testing to provide time-resolved E85 vapor mole fraction measurements at a measurement rate of 40 kS/s, which corresponds to one measurement per crank angle degree at 6700 rpm. Time-resolved and average sensor measurements provide key performance insights for the development of intake systems in high-performance internal combustion engines.

Imbricated structure and hydraulic path induced by strike slip reactivation of a normal fault

In carbonates, fault zones strongly influence local reservoir properties and act as drains or barriers to flows. In the case of reactivated faults, newly developed fracture and fault systems rework and cross-cut pre-existing fault structures. The modifications of initial damage zone and fault core properties alter the fault zone properties and potentially created new fluid pathways. This study of the polyphase Castellas fault zone, developed as normal and later reactivated as strike slip, characterizes the structural pattern related to tectonic reactivation, and estimates the hydraulic behaviour of the fault zone through time. This fault affects carbonates located on La Fare anticlinal (SE France). High resolution mapping, 848 measurements and cross-sections allowed to discriminate the fault structures and the lateral heterogeneity of its architecture. Along the 850m-long studied damage zone we identify 8 principal fault planes, 2 relay zones, 19 secondary fault planes, and fault lenses. The study shows that the fault zone developed as isolated fault segments during the normal activity and acted as a drain to fluid flows during its nucleation. Later strike-slip reactivation led to the main structures visible presently as a well-connected fracture network and un-cemented breccia allowing the fault to be a drain to fluid flows.

The Regional Extents of Local Thrust Systems in Jabbari and Rupper Town, South East of Hazara Pakistan

Geological traverse between the town of Jabbri and Rupper, southeast Hazara delineates the stratigraphic and structural element of the area presented by high resolution geological map and cross section of the area. Oldest rocks in this section is Precambrian Hazara formation which is disconformably overlain by a Jurassic to Eocene sequence including Samana Suk, Chichali, Lumshiwal, Kawagarh, Hangu, Lockhart, Patala, Margalla-hill-limestone, Chorgali and Kuldana formations. Located in Lesser Himalayas, under the influence of main boundary thrust (MBT), this area developed several thrust systems from north to south including Hazara thrust, Haro thrust, Sangoda thrust, Rupper thrust. The main regional Hazara thrust has Precambrian rock in its hanging wall thrusted over the Jurassic Samna Suk formation. In lower detachment, there are several local thrust systems within Jurassic-Eocene strata.These thrusts trends in NE-SW direction indicating NW-SE compressional regime with the hanging wall Drag folds showing southward vergence. The stereographic plot of mesoscopic scale folds shows at least three folding events and varying plunging directions between the range of 240° and 290°. Most of the folds are plunging towards west and this is yet another indicator of east-west compressional forces. These folds orientations suggest transpressional deformation rather than pure compression. The balanced restore section shows that there is about 2.5 km shortening along 15 km restored section which makes about 16% shortening along the section.
 

A hydrological tipping point and onset of Neolithic wetland occupation in Pestenacker (Lech catchment, S Germany)

The reconstruction and understanding of tipping points in environmental systems have become an important issue for the global geoscientific community. This study focuses on the reconstruction of the Holocene deposition history of the Loosbach valley at the Pestenacker site, a Late Neolithic wetland occupation in the northern Alpine forelands of Central Europe (South Germany). Since 2011, it has been part of the UNESCO World Heritage ‘Prehistoric pile dwellings around the Alps’. This study aims to reconstruct a potential passing of a Middle Holocene hydrological tipping point in the Loosbach valley and its interrelation with the Late Neolithic onset of valley occupation at that time. In this context, we make use of chronostratigraphical data sets about the Holocene fluvial dynamics of Loosbach stream from available archaeological excavation reports and spatially enlarge the local model of Holocene stream evolution with high-resolution direct push cross-sections from the proximate fen stratigraphies.As a result, the onset of the Late Neolithic settlement phase corresponds with a local hydrological tipping point at the wetland site of Pestenacker. Increased cooling and climatic humidity led to stream incision into the valley floor that resulted in draining, a local decrease of the groundwater table and the end of fen formation. As an apparently contradictory result of this process, increased water availability and run-off correspond with increased edaphic aridity on the valley floor. The latter may have made it possible to settle the valley floor during the late Neolithic.

From apex to shoreline: fluvio-deltaic architecture for the Holocene Rhine–Meuse delta, the Netherlands

Abstract. Despite extensive research on alluvial architecture, there is still a pressing need for data from modern fluvio-deltaic environments. Previous research in the fluvial-dominated proximal and central Rhine–Meuse delta (the Netherlands) has yielded clear spatial trends in alluvial architecture. In this paper, we include the backwater length to establish architectural trends from apex to shoreline. Channel-belt sand body width / thickness ratios and interconnectedness were determined, and the proportions of fluvial channel-belt deposits, fluvial overbank deposits, organics and intertidal deposits were calculated for the complete fluvio-deltaic wedge based on high-resolution geological cross sections. It was found that the average width / thickness ratio of channel-belt sand bodies in the proximal delta is 5 times higher than in the distal delta. Other down-valley trends include an 80 % decrease in the channel deposit proportion (CDP) and a near-constant proportion of overbank deposits. Additionally, interconnectedness in the proximal delta is 3 times higher than in the distal delta. Based on the Rhine–Meuse dataset, we propose a linear empirical function to model the spatial variability of CDP. It is argued that this relationship is driven by four key factors: channel lateral-migration rate, channel-belt longevity, creation of accommodation space and inherited floodplain width. Additionally, it is established that the sensitivity of CDP to changes in the ratio between channel-belt sand body width and floodplain width (normalized channel-belt sand body width) varies spatially and is greatest in the central and distal delta. Furthermore, the proportion of fluvial channel-belt sands is generally an appropriate proxy for the total sand content of fluvio-deltaic successions, although its suitability as a total sand indicator rapidly fades in the distal delta. Characteristics of the backwater zone of the Rhine–Meuse delta are (1) sand body width / thickness ratios that are lower as a consequence of channel narrowing (not deepening), (2) a rapid increase and then a drop in the organic proportion, (3) an increase in the total sand proportion towards the shoreline, and (4) a drop in the connectedness ratio. For this paper, unique high-resolution quantitative data and spatial trends of the alluvial architecture are presented for an entire delta, providing data that can be used to further improve existing fluvial stratigraphy models.

The Creation of High-Resolution Brain Cross-sections for 3D Printing and Virtual Reality Applications.

Technologies such as 3D printing and virtual/augmented reality have great potential for improving the teaching of highly spatial topics such as neuroanatomy. We created a set of 3D printed and virtual brain cross-sections using a high-resolution MRI dataset. These resources have been made freely available via online repositories. We also report a pilot study of the use of both the physical and virtual specimens in the classroom. Students completed a lab exercise where they used either the 3D printed or virtual brain sections to order a set of axial slices from dorsal to ventral. They then labeled the different structures that they found useful in determining the slices' positions. We measured the students' ability to localize 2D brain cross-sections before and after the lab exercise. Overall, we saw pre- to post-test increases in accuracy on a brain cross-sections task compared to a lecture-based neuroanatomy instruction.

High-Resolution Direct Push Sensing in Wetland Geoarchaeology—First Traces of Off-Site Construction Activities at the Fossa Carolina

Wetland environments, with their excellent conservation conditions, provide geoarchaeological archives of past human activities. However, the subsurface soil is difficult to access due to high groundwater tables, unstable sediments, and the high cost of excavation. In this study, we present a ground-based non- and minimal-invasive prospection concept adapted to the conditions of wetlands. We investigated the Fossa Carolina in South Germany, a canal that was intended in 792/793 AD by Charlemagne to bridge the Central European Watershed. Although the resulting Carolingian banks and the fairway with wooden revetments are very imposing, archaeological traces of off-site construction activities have not been identified hitherto. Based on a geophysically surveyed intensive linear magnetic anomaly parallel to the Carolingian canal, we aimed to prove potential off-site traces of Carolingian construction activities. In this context, we built up a high-resolution cross-section using highly depth-accurate direct push sensing and ground-truthing. Our results showed the exact geometry of the canal and the former banks. Thus, the magnetic mass anomaly could be clearly located between the buried organic-rich topsoil and the Carolingian banks. The thermoluminescence dating showed that the position of the magnetic mass anomaly reflected Carolingian activities during the construction phases, specifically due to heat exposure. Moreover, we found hints of the groundwater supply to the 5-metre wide navigable fairway.

Energy deposition in Saturn’s equatorial upper atmosphere

We construct Saturn equatorial neutral temperature and density profiles of H, H2, He, and CH4, between 10−12 and 1 bar using measurements from Cassini’s Ion Neutral Mass Spectrometer (INMS) taken during the spacecraft’s final plunge into Saturn’s atmosphere on 15 September 2017, combined with previous deeper atmospheric measurements from the Cassini Composite InfraRed Spectrometer (CIRS) and from the UltraViolet Imaging Spectrograph (UVIS). These neutral profiles are fed into an energy deposition model employing soft X-ray and Extreme UltraViolet (EUV) solar fluxes at a range of spectral resolutions (Δλ=4×10−3nm to 1 nm) assembled from TIMED/SEE, from SOHO/SUMER, and from the Whole Heliosphere Interval (WHI) quiet Sun campaign. Our energy deposition model calculates ion production rate profiles through photo-ionisation and electron-impact ionisation processes, as well as rates of photo-dissociation of CH4. The ion reaction rate profiles we determine are important to obtain accurate ion density profiles, meanwhile methane photo-dissociation is key to initiate complex organic chemical processes. We assess the importance of spectral resolution in the energy deposition model by using a high-resolution H2 photo-absorption cross section, which has the effect of producing additional ionisation peaks near 800 km altitude. We find that these peaks are still formed when using low-resolution (Δλ=1nm) or mid-resolution (Δλ=0.1nm) solar spectra, as long as high-resolution cross sections are included in the model.

Creating High-Resolution Microscopic Cross-Section Images of Hardwood Species Using Generative Adversarial Networks.

Microscopic wood identification plays a critical role in many economically important areas in wood science. Historically, producing and curating relevant and representative microscopic cross-section images of wood species is limited to highly experienced and trained anatomists. This manuscript demonstrates the feasibility of generating synthetic microscopic cross-sections of hardwood species. We leveraged a publicly available dataset of 119 hardwood species to train a style-based generative adversarial network (GAN). The proposed GAN generated anatomically accurate cross-section images with remarkable fidelity to actual data. Quantitative metrics corroborated the capacity of the generative model in capturing complex wood structure by resulting in a Fréchet inception distance score of 17.38. Image diversity was calculated using the Structural Similarity Index Measure (SSIM). The SSIM results confirmed that the GAN approach can successfully synthesize diverse images. To confirm the usefulness and realism of the GAN generated images, eight professional wood anatomists in two experience levels participated in a visual Turing test and correctly identified fake and actual images at rates of 48.3 and 43.7%, respectively, with no statistical difference when compared to random guess. The generative model can synthesize realistic, diverse, and meaningful high-resolution microscope cross-section images that are virtually indistinguishable from real images. Furthermore, the framework presented may be suitable for improving current deep learning models, helping understand potential breeding between species, and may be used as an educational tool.

Machine learning of geological details from borehole logs for development of high-resolution subsurface geological cross-section and geotechnical analysis

ABSTRACT The subsurface geological cross-section is indispensable before design and construction of a geotechnical structure can commence. The development of geological cross-sections often requires significant manual efforts for simplification of stratigraphic boundaries. For example, straight lines are commonly used to connect stratum boundaries at adjacent boreholes, and soil layers with small thicknesses are often ignored. Such a simplification heavily relies on practitioners’ experience and may induce great uncertainties in the developed geological cross-sections and subsequent geotechnical analysis and design (e.g. slope stability analysis). In this study, a Bayesian supervised machine learning method, multiple-point statistics, is adopted to automatically generate high-resolution subsurface geological cross-section with proper incorporation of prior geological knowledge and all details observed from limited borehole logs. Locations and number of boreholes are automatically determined with due consideration of slope failure mechanism. The proposed method is demonstrated using an illustrative example. It is shown that the proposed method successfully captures geological details and local stratigraphic variations within a slope and quantifies the associated interpretation uncertainties. The geological details and local stratigraphic variations have great effects on the slope failure mechanism. There is a risk of overestimating overall slope stability if an over-simplified geological model is adopted for slope design.

Photoionization Cross-Sections of Carbon-Like N+ Near the K-Edge (390–440 eV)

High-resolution K-shell photoionization cross-sections for the C-like atomic nitrogen ion (N+) are reported in the 398 eV (31.15 Å) to 450 eV (27.55 Å) energy (wavelength) range. The results were obtained from absolute ion-yield measurements using the SOLEIL synchrotron radiation facility for spectral bandpasses of 65 meV or 250 meV. In the photon energy region 398–403 eV, 1s⟶2p autoionizing resonance states dominated the cross section spectrum. Analyses of the experimental profiles yielded resonance strengths and Auger widths. In the 415–440 eV photon region 1s⟶(1s2s22p2 4P)np and 1s⟶(1s2s22p2 2P)np resonances forming well-developed Rydberg series up n=7 and n=8 , respectively, were identified in both the single and double ionization spectra. Theoretical photoionization cross-section calculations, performed using the R-matrix plus pseudo-states (RMPS) method and the multiconfiguration Dirac-Fock (MCDF) approach were bench marked against these high-resolution experimental results. Comparison of the state-of-the-art theoretical work with the experimental studies allowed the identification of new resonance features. Resonance strengths, energies and Auger widths (where available) are compared quantitatively with the theoretical values. Contributions from excited metastable states of the N+ ions were carefully considered throughout.