Intake Of High-quality Protein Research Articles

Using low-protein diet in egg production for win-win of productivity and environmental benefits should be prudent: Evidence from pilot test

A shortage of feed protein resources restricts poultry productivity. Key strategies to alleviate this problem include improvements to the structure of the gut microbiota by the appropriate intake of high-quality protein, improvements to the comprehensive protein utilization rate, and reducing the consumption of protein raw materials. In addition, damage to the environment caused by nitrogen emissions needs to be reduced. The aim of the study was to evaluate the effects of dietary protein levels on laying performance, host metabolism, ovarian health, nitrogen emissions, and the gut microbial structure and function of laying hens. In total, 360 hens at the age of 38 weeks were randomly allotted four treatments. Each of the groups consisted of nine replicates, with 10 birds per replicate, used for 12 weeks of study. Dietary protein levels of the four groups were 13.85 %, 14.41 %, 15.63 %, and 16.30 %. Results revealed that, compared with the 13.85 % crude protein (CP) group, the 15.63 % CP group experienced significantly enhanced final body weight, average daily gain, egg production, and egg mass. Compared with the 16.30 % CP group, the other groups' serum concentrations of immunoglobulin G (IgG) and immunoglobulin M (IgM) were significantly reduced. Compared with the 16.30 % CP group, the 13.85 % and 15.63 % groups had increased CP utilization rates but reduced nitrogen emission rate, and daily per egg and per kilogram egg nitrogen emissions rose with increased dietary protein levels. Compared to the 13.85 % and 14.41 % CP groups, the 16.30 % CP group exhibited a significant increase in the expression of genes related to amino acids and carbohydrate metabolic pathways. According to the linear discriminant analysis effect size diagram, the predominant bacteria in the 15.63 % CP group (e.g., Subdoligranulum, and Ruminococcaceae_UCG-013) were significantly related to CP utilization. The results of this study emphasize that production performance is significantly reduced when protein levels are too low, whereas too high protein levels lead to gut microbiota imbalance and a reduction in the utilization efficiency of nutrients. Therefore, on the premise of ensuring the health of hens, the structure of the gut microbiota can be improved by appropriately reducing protein levels, which helps to balance the relationships among host health, productivity, resources, and the environment.

A cluster randomized trial of a comprehensive intervention nesting family and clinic into school centered implementation to reduce myopia and obesity among children and adolescents in Beijing, China: study protocol

BackgroundMyopia and obesity in children and adolescents have become serious public health problems that endanger public health, especially in China. Unhealthy lifestyle behaviors are environmental drivers of both myopia and obesity. This protocol describes a study to evaluate the effectiveness of “22510SS”, that is 2 h of daytime outdoor activities (‘2’); Limit screen time to no more than 2 h per day (‘2’); Consume at least 5 servings of fruits and vegetables daily (‘5’); Attain 1 h of physical activity daily (‘1’); Consume 0 sugar-sweetened beverages (‘0’); Reasonable sleep duration (‘S’); Regular supervision (‘S’). A school-based, multifaceted intervention strategy for myopia and obesity prevention, and to assess and explore the implementation of “22510SS” with regards to acceptability, feasibility, adoption, usage and maintenance.Methods and analysisThis study aims to develop a comprehensive intervention strategy "22510SS" based on the socio-ecological model, and A two-arm cluster randomized trial with a parallel-group of a 1:1 allocation ratio in 36 primary and secondary schools to test its evidence-based intervention programs on the effects and implementation of myopia and obesity epidemics in children and adolescents in grades 4 and 7. The primary outcomes will include differences in visual acuity, body mass index, outdoor activity indicators, screen time, fruit and vegetable intake, high-quality protein intake, sugar-sweetened beverage intake, sleep duration, and level of monitoring among children and adolescents. Secondary outcomes will assess the acceptability, feasibility, uptake, use, and maintenance of the intervention. Effects on the primary and secondary outcomes will be analyzed using linear and logistic regression analyses, as well as difference-in-difference analysis, taking into account cluster effects and possible confounding factors. Process assessments will also be conducted through quantitative and qualitative analyses, including acceptability, feasibility, gender, adoption, implementation, and sustainability.DiscussionThis study will evaluate the effectiveness of “22510SS” and examine its implementation in the school-based network nesting family and clinic. Following this intervention study, the integrated intervention program focused on myopia and obesity among children and adolescents have great potential to be implemented in China to promote and support healthy lifestyle behavior change and reduce the risk of myopia and obesity in children and adolescents.Trial registrationNCT05275959. Registered 23 Mach 2022.

Management of Type 2 Diabetes in Frail Older Adults.

Aging is one of the most important factors associated with the dramatic increase in the prevalence of type 2 diabetes mellitus (T2DM) globally. In addition to traditional micro- and macrovascular complications, diabetes mellitus (DM) in older adults is of great importance due to its independent relationship with frailty, which is defined as a decline in functional reserves and vulnerability to stressors. Frailty assessment enables the determination of biological age, thus predicting potential complications in older adults and identifying tailored treatment strategies. Although the latest guidelines have acknowledged the frailty concept and provided recommendations specific to this subgroup of older adults, frail older adults are particularly considered only as anorexic, malnourished people for whom relaxed treatment targets should be set. However, this approach bypasses other metabolic phenotypes in the context of diabetes and frailty. Recently, a spectrum of metabolic phenotypes in the context of frailty in DM was suggested, and the two edges of this spectrum were defined as "anorexic malnourished (AM)" and "sarcopenic obese (SO)." These two edges were suggested to require different strategies: Opposite to the AM phenotype requiring less stringent targets and de-intensification of treatments, tight blood glucose control with agents promoting weight loss was recommended in the SO group. Our suggestion is that, regardless of their phenotype, weight loss should not be the primary goal in DM management in older adults who are overweight or obese, because of the increased malnutrition prevalence in older adults suffering from DM compared with standard older adults. Furthermore, overweight older adults have been reported to have the lowest risk of mortality compared with other groups. On the other hand, obese older individuals may benefit from intensive lifestyle interventions including caloric restriction and regular exercise with the assurance of at least 1 g/kg/day high-quality protein intake. Besides metformin (MF), sodium-glucose cotransporter-2 inhibitors (SGLT-2i) or glucagon-like peptide-1 receptor agonists (GLP-1RA) should be considered in appropriate SO cases, due to high evidence of cardiorenal benefits. MF should be avoided in the AM phenotype due to their weight loss property. Although weight loss is not desired in AM phenotype, SGLT-2i may still be preferred with close follow-up in certain individuals demonstrating high cardiovascular disease (CVD) risk. Of note, SGLT-2i should be considered earlier in the diabetes treatment in both groups due to their multiple benefits, i.e., organ protective effects, the potential to reduce polypharmacy, and improve frailty status. The concept of different metabolic phenotypes in frail older adults with diabetes once again shows "one size fits all" cannot be applied in geriatric medicine, and a tailored, individualized approach should be adopted to get the highest benefit from treatments.

The Effect of Soy and Whey Protein Supplementation on Glucose Homeostasis in Healthy Normal Weight Asian Indians.

Milk and legumes are good source of protein foods used to sustain muscle mass, but their effects on postprandial glucose homeostasis and energy metabolism may be different. This is relevant, for example, in the dietetic response to obesity or diabetes, where the intake of high-quality protein is often increased significantly. The objective of this study was to characterize the acute effect of whey and soy protein (15% vs. 30%) on glucose homeostasis, energy metabolism, and satiety. Healthy, normal body mass index (BMI) Indian adult males aged 20-35 years (n = 15) received 4 test meals (2 proteins (soy vs. whey) and 2 doses (15% vs. 30% protein: energy ratio)). Blood samples were collected serially after the meal to calculate the incremental area under the curve for plasma glucose and insulin. Energy expenditure and substrate oxidation were measured after the meal. Satiety was measured with a visual analogue scale. The insulin response, represented by the incremental area under the curve, was significantly higher for the 30% whey compared to the 30% soy protein meal (p < 0.01) but was not significantly different between the 15% protein doses. There were no differences in the plasma glucose response across protein sources or doses. The mean peak fat and carbohydrate oxidation, satiety, and energy expenditure did not differ between the protein sources and doses. In conclusion, at higher doses, whey protein has a greater insulinogenic response, compared to soy protein, and exhibits a dose-response effect. However, at lower doses, whey and soy protein elicit similar insulinogenic responses, making them equally effective protein sources in relation to glucose homoeostasis.

Bifidobacterium as a Potential Biomarker of Sarcopenia in Elderly Women.

Gut microbial dysbiosis influences the development of sarcopenia. This case-control study explored the gut microbiota composition in elderly Chinese women with sarcopenia. The information from 50 cases and 50 controls was collected. Grip strength, body weight, body mass index, skeletal muscle mass, energy intake, and total and high-quality protein intake were lower in cases than in controls (p < 0.05). Gut microbiota metagenomic sequencing showed that phylum Bacteroides was significantly reduced in the case group, whereas genus Prevotella was more abundant (p < 0.05). Linear discriminant analysis (LDA) effect size showed that 9 and 13 distinct microbial taxa were enriched in the case and control groups, respectively (LDA > 2, p < 0.05), among which Prevotella copri and Bifidobacterium longum were significantly different (LDA > 4, p < 0.05). The AUC of Bifidobacterium longum was 0.674 (95% CI: 0.539-0.756). Elderly women with sarcopenia exhibited significantly different gut microbiota compositions than healthy controls.

Dietary pattern characterized by a balanced diet rich in high-quality protein intake is associated with mild initial clinical manifestations in tuberculosis.

BackgroundThe relationship between a single food or nutrient and pulmonary tuberculosis (TB) has been explored in many studies; however, the relationship between dietary patterns and TB is still lacking.ObjectiveOur study aims to investigate the association between dietary patterns and the initial clinical manifestations in patients with TB.Materials and methodsA cross-sectional study including 1,661 patients with active TB was conducted in Qingdao, China, from 2011 to 2019. A semiquantitative food frequency questionnaire was used to collect dietary data. Dietary patterns were determined by principal component factor analysis. Initial clinical manifestations were assessed using a combination of the patient self-reported clinical symptoms and the admission results indicated by the TB score. The associations between dietary patterns and TB scores in patients with TB were examined by the logistics regression model.ResultsThe analysis identified four dietary patterns: meat-fruit-seafood pattern; dairy-egg pattern; beans and their products-whole grain pattern; and refined grain-vegetable pattern. In a multiple-adjusted model, higher adherence to the meat-fruit-seafood pattern showed a protective effect on the TB score (OR 0.53, 95% CI 0.39, 0.84, P for trend = 0.010) and the association was stronger in patients older than 45 years (OR 0.32, 95% CI 0.16, 0.64, P for trend < 0.001). The higher adherence to beans and their products-whole grain pattern was a protective factor for TB score (OR 0.57, 95% CI 0.37, 0.87, P for trend = 0.025), and the association was also observed in patients with concurrent TB and diabetes mellitus (DM) with a more significant effect (OR 0.33, 95% CI 0.14, 0.80, P for trend = 0.025). No significant association was found between dairy-egg pattern and refined grain–vegetable dietary pattern with TB score.ConclusionDietary patterns characterized by a balanced diet rich in high-quality protein, sufficient energy, as well as marine n-3 PUFA, phytochemicals, B vitamins, and fiber are associated with mild initial clinical manifestations, and the association is stronger in patients older than 45 years and those with concurrent TB and DM.

Energy and macronutrient intakes of employees at different levels of work intensity in China from 2010 to 2012

To analyze the intake of energy and macronutrients of employees at different levels of work intensity in China. Based on the data of National Nutrition and Health Survey in 2010-2012, this paper analyzed the energy and macro nutrients intakes of employees at three levels of working strength. The energy intake of employees in China was 1952. 7 kcal/d, the intakes of protein, fat and carbohydrate were 62. 7 g/d, 76. 6 g/d and 254. 0 g/d, respectively, the energy supply ratios were 13. 1%, 34. 9% and 52. 4%, respectively. From food sources of energy, the percentage of total energy provided by cereals and animal foods was 47. 7% and 18. 2%, respectively. The percentage of protein from cereal, legumes and Animal food was 39. 8%, 6. 7% and 37. 5%, respectively. The percentage of fat from animal food was 34. 8%. From the age and work intensity groups, the energy intake was lower in the low age group and the light work intensity of employees. There was the lowest intake of protein and fats in high age groups with heavy work intensity. Energy levels form protein and fat were highest in the light intensity group. Energy supply from cereal food was the highest in the group with heavy work intensity and energy supply from animal food was the highest in the group with low age group and light work intensity. The intake of high quality protein and animal food fat decreased with the increase of age and work intensity. The energy intake of employees in China is lower than the recommended energy intake for Chinese residents, and fat provides a higher proportion of energy. Employees with high age and heavy work intensity have insufficient protein intake, and the unreasonable dietary structure was particularly prominent with them, and their nutritional status needs to be paid more attention.

The Facts about Food after Cancer Diagnosis: A Systematic Review of Prospective Cohort Studies.

Nutritional guidelines suggest specific energy and protein requirements for patients with cancer. However, cancer patients, often malnourished, use self-made or web-based diets to ameliorate the prognosis of their disease. This review aimed to investigate the associations between post-diagnostic diet and prognostic outcomes in cancer patients. A systematic literature search was performed in Pubmed and Web of Science databases from inception to 30 October 2019, based on fixed inclusion and exclusion criteria. The risk of bias was assessed. A total of 29 prospective studies was identified. Breast (n = 11), colorectal (n = 9), prostate (n = 8) cancers are the most studied. Low- fat diet, healthy quality diet, regular consumption of fiber such as vegetables and high-quality protein intake are beneficial while Western diet (WD) and high consumption of saturated fats could be associated with a higher risk of mortality. Bladder (n = 1), gynecological (n = 1), lung, stomach, and pancreatic cancers still remain almost unexplored. This systematic review suggested that detrimental dietary patterns such as WD should be avoided but none of the food categories (meat, dairy products) should be eliminated in cancer patients’ diet. Further large prospective studies are needed to assess the role of post-diagnostic diet in patients with cancer.

Analysis of dietary and metabolic characteristics in elderly sarcopenia patients with diabetes mellitus

Objective To investigate the metabolic characteristics, body composition and dietary intake in elderly sarcopenia patients with type 2 diabetes(T2DM). Methods A total of 652 T2DM patients(327 males and 325 females)aged over 60 years were selected from endocrinology department of nine different hospitals in Beijing.Body composition was measured by bioimpedance analysis, and the appendicular skeletal mass index(ASMI)was calculated.Sarcopenia was defined as body height-adjusted skeletal muscle mass(ASMI)below the lower quintile of the young reference group.The diagnostic cutoff points for sarcopenia were 7.18 kg/m2 for men and 5.73 kg/m2 for women.All patients were divided into the sarcopenia group versus the control group(below vs.not below 7.18 kg/m2 for men and 5.73 kg/m2 for women). The anthropometric parameters, body composition, biochemical laboratory results and dietary intake were compared between the two groups.The blood glucose target levels were less than 7 mmol/L of fasting plasma glucose(FPG)or less than 7% of haemoglobin A1c(HbA1c). The obesity ratio was calculated according to body fat percentage more than 25% in men and more than 30% in women as obesity. Results There were 116(17.8%)patients in the sarcopenia group(men/women, 82/34), and 536(82.2%)patients in the control group(men/women, 245/291). Age was higher in the sarcopenia group than in the control group(t=4.385, P=0.000), and body mass index and waist hip ratio(WHR)were lower in the sarcopenia group than in the control group(t=11.724 and 4.173, P=0.000 and 0.000). FPG[(7.5±2.4)mmol/L vs.(8.5±2.5)mmol/L, t=-3.450, P=0.001]and HbA1c[(7.0±1.6)% vs.(8.2±1.7)%, t=-5.745, P=0.000]were higher in male sarcopenia group than in male control group.The control rate of FPG(127.0% or 51.8% vs.27.0% or 32.9%, χ2=8.817, P=0.003)and HbA1c(131.0% or 53.5% vs.23.0% or 28.0%, χ2=15.934, P=0.000)were lower in the sarcopenia group than in the control group.The red blood cell counts, hemoglobin and serum albumin levels, estimated glomerular filtration rate(eGFR)were lower in male sarcopenia group than in the male control group(P<0.05). eGFR was lower in female sarcopenia group than in female control group(t=4.090, P=0.000). Both in men and women, ASMI, grip power, fatless circumference on upper arm, bone mineral content and basal metabolic rate were lower in the sarcopenia group than in the control group(P<0.05). The total daily intake of energy, carbohydrate, protein and fat were lower in male sarcopenia group than in male control group(P<0.05). Conclusions Compared with the control group, sarcopenia patients are older with worse glycemic control and lower levels of BMI, WHR, renal function, muscle mass and muscle strength.Sarcopenia patients are more prone to osteoporosis.Furthermore, they have poorer nutritional status with an imbalance of dietary intake.Appropriate increase of protein especially high quality protein intake should be recommended to sarcopenia patients with T2DM. Key words: Diabetes mellitus; Sarcopenia; Nutrition assessment; Metabolism

Bread from wheat flour partially replaced by fermented chickpea flour: Optimizing the formulation and fuzzy analysis of sensory data

The use of legume flours to supplement a staple food like wheat bread has immense potential, particularly in developing countries to increase the dietary intake of high quality protein. The study aimed to develop a functional wheat bread with fermented chickpea flour (FCF). The solid state fermentation conditions (1–2% yeast extract) of chickpea flour were studied upto 5 days. The minimum phytic acid content with a maximum free amino acids was obtained at 1.4% yeast extract when the flour was fermented for 83 h. The amount of FCF as a replacement of wheat flour (WF) was optimized further using D-optimal mixture design. The three constituents varied in the formulations were WF (67–94.5%), FCF (5–30%) and xanthan gum (0.5–3%), respectively. The responses analyzed were hardness, total color change in both crust and crumb, and specific loaf volume of the bread. The numerically optimized proportion in mass fraction of WF: FCF: xanthan gum was 80:18:2 having the desirability value of 0.73. Concomitantly, fuzzy analysis of the same combinations affirmed the suitability of the optimized formulation, as reflected by the similarity value (0.745).

Nutrition as a Metabolic Modulator in COPD

COPD is an important global health problem. In addition to pulmonary impairment, systemic inflammation, musculoskeletal abnormalities, and cardiovascular comorbidity influence disease burden and mortality risk. Body weight and body composition are important discriminants in classifying disease heterogeneity. The rationale for and efficacy of caloric supplementation in preventing and treating involuntary weight loss is currently well established. For maintenance of muscle and bone tissue, appropriately timed, high-quality protein intake and addressing vitamin D deficiency must be considered. Specific nutrients (eg, n-3 polyunsaturated fatty acids and polyphenolic compounds) may have the pharmacologic potential to boost decreased muscle mitochondrial metabolism and enhance impaired physical performance, particularly when the metabolic stimulus of physical activity alone is limited. At this stage, evidence is insufficient to support an intake of high doses of single nutritional supplements to modulate respiratory pathology, but some small studies have identified micronutrient modulation via the diet as a promising intervention.

Increased Lean Mass with Reduced Fat Mass in an Elite Female Cyclist Returning to Competition: Case Study

Body composition in a female road cyclist was measured using dual-energy X-ray absorptiometry (5 occasions) and anthropometry (10 occasions) at the start of the season (Dec to Mar), during a period of chronic fatigue associated with poor weight management (Jun to Aug), and in the following months of recovery and retraining (Aug to Nov). Dietary manipulation involved a modest reduction in energy availability to 30-40 kcal · kg fat-free mass(-1) · d(-1) and an increased intake of high-quality protein, particularly after training (20 g). Through the retraining period, total body mass decreased (-2.82 kg), lean mass increased (+0.88 kg), and fat mass decreased (-3.47 kg). Hemoglobin mass increased by 58.7 g (8.4%). Maximal aerobic- and anaerobic-power outputs were returned to within 2% of preseason values. The presented case shows that through a subtle energy restriction associated with increased protein intake and sufficient energy intake during training, fat mass can be reduced with simultaneous increases in lean mass, performance gains, and improved health.

Trends in dietary protein intake among Chinese children and adolescent between 1991 and 2009

To examine dietary protein intake and its trend between 1991 and 2009 among Chinese children and adolescents aged 6 to 17 years. Consecutive 3 d-24 hr Dietary Recall and household income data of 14 053 children from the seven waves of the China Health and Nutrition Survey (CHNS), 1991, 1993, 1997, 2000, 2004, 2006 and 2009 were used to describe long-term changes in dietary protein intake, percentage energy, and food sources. The median of dietary protein intake of Chinese children and adolescents decreased from 60.0 g/d in 1991 to 52.6 g/d in 2009 (χ² = 109.0, P < 0.01). The dietary protein intake among children in rural areas and in low-income households decreased 8.8 g/d (χ² = 74.8, P < 0.01) and 9.8 g/d (χ² = 100.5, P < 0.01) respectively. The percentage of total energy intake from protein was 11.3%-13.0%. The percentage of protein intake equal or higher than Recommended Nutrient Intake of protein decreased from 29.5% in 1991 to 19.2% in 2009 (χ² = 259.6, P < 0.01). Furthermore, the proportion of high-quality dietary protein increased rapidly. The percentage of animal protein increased from 12.3% in 1991 to 32.2% in 2009 (χ² = 750.3, P < 0.01), while no big increase in soybean protein (lower than 5.0%). There is a decreasing trend in dietary protein intake among Chinese children from 1991 to 2009. The high-quality protein intake was improved but we should be concerned about the high proportion of animal protein. The children living in low-income households and in rural areas may be at risk of inadequate dietary protein.

An Analysis on Dietary and Nutritional Status of Shandong Residents, China

To know the dietary and nutritional status of Shandong residents, we used data of the China National Nutrition Survey, 3284 participants (1433 in urban, l185 in rural) from 1285 households (545 in urban, 740 in rural), drawn in Shandong province by using a stratified cluster random sampling method. Dietary data were collected by a combination of weighing method and 3 consecutive 24‐h recalls. Compared to the survey in 1992, intake of cereals and starch tubers decreased while the intake of animal food has increased quickly in 2002. Intakes of milk, eggs, fish, shellfish, and lipids in Shandong residents are obviously higher than the national average. Intake of cereals is 68.6g per day in urban areas, less than in rural areas. Intake of animal foods is 97g per day in urban areas, much higher than in rural areas. Urban residents consumed less energy, protein, fat, carbohydrate, vitamin C and sodium than their rural peers did. The change of the dietary pattern of Shandong residents lies mainly on decrease in intake of cereals and starch tubers and increase in intake of animal food and high‐fat diet. Residents in urban areas experienced faster shift in diet toward high‐fat diet. The greater consumption of meat, poultry, eggs and milk bring increase in intake of high quality protein, but residents’ diet in Shandong is still unbalanced. More nutritional education should be done to guide reasonable dietary.

The effect of dietary protein on reproduction in the mare. III. Ovarian and uterine changes during the anovulatory, transitional and ovulatory periods in the non-pregnant mare.

In the main experiment the total daily protein intake and quality (essential amino-acids) was varied in 4 groups of mares. The incidence of oestrus in mares during the transitional period was unaffected by protein nutrition. Ovarian activity, as evaluated by follicular development and size of the ovaries, was affected. Mares that received low-quality protein (Groups 1 and 2) had a higher number of smaller follicles (< 10 mm) that developed during the transitional period compared to mares on a high-quality protein intake (Groups 3 and 4). The mares that received the high quality protein ovulated 2-3 weeks earlier in the breeding season in a synchronised period of 4-5 weeks compared to a period of 6-8 weeks in Groups 1 and 2. The duration of the subsequent oestrous cycles was not affected. There was no difference in the diameter of the largest follicle of mares between groups on the day before ovulation. In a separate experiment, 5 maiden Anglo-Arab mares, 4-5 years of age, were slaughtered at different stages during the anovulatory, transitional and ovulatory periods of the breeding cycle. The morphology of the ovaries and uteri of these mares was described and photographed for use as guidelines when comparing ovarian changes and follicular activity of mares.