High Pumping Rates Research Articles

Groundwater Model for Karst and Pelitic Aquifer Systems from a Semi-Arid Region Under Climate Change Scenarios: A Case Study in the Vieira River Watershed, Brazil

Water scarcity is a global issue, especially in semi-arid and arid regions where precipitation is irregularly distributed over time and space. Predicting groundwater flow in heterogeneous karst terrains, which are essential water sources, presents a significant challenge. This article integrates geology, hydrology, and water monitoring to develop a pioneering conceptual and numerical model of groundwater flow in the Montes Claros Region (Vieira River Watershed, Brazil). This model was evaluated under various climate change scenarios, considering changes in rainfall, groundwater consumption, and population growth over the current century. The results indicate that a decline in water table levels is inevitable, primarily driven by population growth and high pumping rates rather than rainfall fluctuations. This underscores the urgent need for improved monitoring, model upgrading, and more importantly, targeted water resource management for Montes Claros.

Numerical investigation into the acid flow and reaction behavior in the tight, naturally fractured carbonate reservoir during acid fracturing

Tight naturally fractured carbonate reservoirs require acid fracturing to build the connectivity between the wellbore and natural fractures (NFs), where hydrocarbon is stored. The high leakoff nature of the NF complicates the acid flow and etching pattern, raising the difficulty in acid fracturing design and optimization. To explore the acid flow and reaction behavior in such reservoirs, an acid fracturing model accounting for the NF distribution is developed, which consists of a fracture surface characterization model, a fracture propagation model, and an acid-etching model. Based on the model, the effects of injection parameters and the NF properties on the effectiveness of acid fracturing are investigated. Then, strategies for acid fracturing the tight naturally fractured carbonate reservoirs are proposed. Results show that high pumping rates and retarded acids with low hydrogen ion (H+) diffusion coefficient is conducive to achieving a long acid penetration distance, while a low pumping rate and acids with a high H+ diffusion coefficient facilitates the NF etching. Therefore, a large stimulated area can be achieved by applying a multi-stage alternating injection of the crosslinked acid with a high pumping rate followed by the gelled acid with a low injection rate. NFs impact acid fracturing in two distinct ways: enhancing the non-uniform etching of the fracture surface and reducing the effective acid-etched fracture length through high leakoff. When NF density is high, leakoff control techniques should be employed; and when the NF inclination is high, non-uniform etching techniques should be used to generate acid-etched channels in flow barriers.

Aquiferous system, filtration rates and hydrodynamics of the syconoid calcareous sponge Urna sp.

The aim of the present study was to gain insight into the hydrodynamic characteristics of the relatively simple aquiferous system in specimens of a calcareous syconoid sponge, Urna sp. Data on the morphology and ultrastructure of the sponge combined with measured pumping rates were provided and used for subsequent estimates of the pressure drops of water flow through the aquiferous system. The pumping rates were estimated from microscope video-recordings as the product of osculum-cross sectional area and exhalant jet speed. Estimates are given of the sensitivity of pressure drops to dimensional changes associated with observed dynamic, contractile structures (e.g., osculum, apopyles), as well as possible artefacts introduced in the preparations for ultrastructural studies. The estimated pressure losses showed that the choanocyte pumps provide a pressure of 5.5 ± 3.9 Pa at a pumping rate of 1533 ± 1089 µm3 s−1 per choanocyte. Such high pumping rates, comparable to those of some choanoflagellates, have not been reported for syconoid and leuconoid sponges before. However, the corresponding sponge volume-specific pumping rates (about 10–30 min−1) are comparable to values reported in the literature for small sponges and explants that also have relatively higher pumping rates of choanocytes than larger sponges.

Fracture-controlled fracturing mechanism and penetration discrimination criteria for thin sand-mud interbedded reservoirs in Sulige gas field, Ordos Basin, China

Considering the problems in the discrimination of fracture penetration and the evaluation of fracturing performance in the stimulation of thin sand-mud interbedded reservoirs in the eighth member of Shihezi Formation of Permian (He-8 Member) in the Sulige gas field, a geomechanical model of thin sand-mud interbedded reservoirs considering interlayer heterogeneity was established. The experiment of hydraulic fracture penetration was performed to reveal the mechanism of initiation–extension–interaction–penetration of hydraulic fractures in the thin sand-mud interbedded reservoirs. The unconventional fracture model was used to clarify the vertical initiation and extension characteristics of fractures in thin interbedded reservoirs through numerical simulation. The fracture penetration discrimination criterion and the fracturing performance evaluation method were developed. The results show that the interlayer stress difference is the main geological factor that directly affects the fracture morphology during hydraulic fracturing. When the interlayer stress difference coefficient is less than 0.4 in the Sulige gas field, the fractures can penetrate the barrier and extend in the target sandstone layer. When the interlayer stress difference coefficient is not less than 0.4 and less than 0.45, the factures can penetrate the barrier but cannot extend in the target sandstone layers. When the interlayer stress difference coefficient is greater than 0.45, the fractures only extend in the perforated reservoir, but not penetrate the layers. Increasing the viscosity and pump rates of the fracturing fluid can compensate for the energy loss and break through the barrier limit. The injection of high viscosity (50–100 mPa·s) fracturing fluid at high pump rates (12–18 m3/min) is conducive to fracture penetration in the thin sand-mud interbedded reservoirs in the Sulige gas field.

Extreme massive hydraulic fracturing in deep coalbed methane horizontal wells: A case study of the Linxing Block, eastern Ordos Basin, NW China

Deep coal seams show low permeability, low elastic modulus, high Poisson’s ratio, strong plasticity, high fracture initiation pressure, difficulty in fracture extension, and difficulty in proppants addition. We proposed the concept of large-scale stimulation by fracture network, balanced propagation and effective support of fracture network in fracturing design and developed the extreme massive hydraulic fracturing technique for deep coalbed methane (CBM) horizontal wells. This technique involves massive injection with high pumping rate + high-intensity proppant injection + perforation with equal apertures and limited flow + temporary plugging and diverting fractures + slick water with integrated variable viscosity + graded proppants with multiple sizes. The technique was applied in the pioneering test of a multi-stage fracturing horizontal well in deep CBM of Linxing Block, eastern margin of the Ordos Basin. The injection flow rate is 18 m3/min, proppant intensity is 2.1 m3/m, and fracturing fluid intensity is 16.5 m3/m. After fracturing, a complex fracture network was formed, with an average fracture length of 205 m. The stimulated reservoir volume was 1 987×104 m3, and the peak gas production rate reached 6.0×104 m3/d, which achieved efficient development of deep CBM.

Global analysis of groundwater pumping from increased river capture

In many regions globally, groundwater overuse exceeds natural replenishment, leading to immediate consequences such as reduced river flows and devastating impacts on freshwater ecosystems. In alluvial aquifers in particular, groundwater pumping contributes to river flow reduction in two significant ways: first, by intercepting water that would naturally discharge into the river, and second, by lowering groundwater levels below the riverbed, causing river water to infiltrate. Despite these critical interactions, large-scale water resources assessments often overlook the relationship between groundwater and surface water, hindering a comprehensive understanding of the consequences of groundwater pumping on both the groundwater and surface water systems. Our study, utilizing a coupled global-scale groundwater–surface water model, reveals that approximately 20% of globally pumped groundwater stems from diminished streamflow, while 16% results from reduced storage. Projections for the end of the century, accounting for climate change, suggest potential increases to 30% from reduced streamflow and a decrease to 12% from reduced storage. Notably, our results highlight that the impact on streamflow is more widespread and linked to smaller pumping rates, contrasting with impacts on storage associated with higher pumping rates. This study shows the crucial need to include groundwater–surface water interactions in large-scale water resources assessments, not only for accurate estimates of freshwater availability but also for a comprehensive understanding of the far-reaching impacts of groundwater overuse related to increasing water demands and climate change.

Mud infiltration and travel paths during mud loss experiments on horizontal borehole stability

Excessive mud pressure may endanger stability of horizontal boreholes and the success of drilling and reaming processes during Horizontal Directional Drilling (HDD). However, no laboratory studies have been undertaken to investigate the relationship between horizontal borehole instability (resulting in mud loss rather than borehole collapse) and mud infiltration and travel paths from the borehole to the ground surface. In this paper, exhumations after a series of mud loss experiments in a medium-scale test pit are used to explore mud infiltration into the sand surrounding the borehole, and the paths used to travel from the borehole to the ground. Different factors are investigated including burial depths, pump rates and the effect of multi-layered soil systems (dense sand covered by different thicknesses of loose sand or dense sandy gravel). Details of the mud travel paths from the experiments are presented. Water contents and average radial extent of mud infiltration around the boreholes are summarized. It is concluded that shear failure of the sand has a dominant role in defining the mud transport through coarse-grained soil. A primary crack appearing on the ground surface approximately above and parallel to the borehole, and secondary cracks transverse to the primary cracks then developed, linking the volumetric expansion of the sand due to mud intrusion and uplift at the ground surface. Ground movements are more dramatic in experiments involving high pump rate but the movements were reduced in experiments involving multi-layered soil systems.

Electrostatic-hydraulic coupled soft actuator for micropump application

The development of a soft actuator with high displacement is crucial for the effective operation of micropumps, ensuring a high fluid pump rate. This study introduces an innovative approach by presenting the design and fabrication of a novel electrostatic-hydraulic coupled soft actuator for a micropump within a microfluidic system. This pioneering soft actuator, leveraging electrostatic-hydraulic coupling, showcases a unique solution to enhance the performance of micropumps. The versatility of such a soft actuator makes it particularly promising for biomedical applications. The actuator comprises dielectric fluid in an elastomeric shell and electrodes to form the out-of-plane fluid-amplified displacement. This displacement amplification was used to generate a pumping actuation in the micropump. The actuator was characterized in terms of dielectric fluid volume, electrode size, temporal response, and amplification displacement. The soft actuator showed a maximum amplified displacement of 0.51 mm at 10 kV of the applied voltage, but a higher voltage caused a dielectric breakdown. Moreover, the actuator demonstrated the ability to operate at frequencies of 0.25 Hz and 0.1 Hz. The results of the study indicate that the fabricated electrostatic-hydraulic coupled soft actuator is a dependable and effective method of actuation for a micropump in a microfluidic system. The experimental characterization of the micropump revealed a maximum flow rate of 2304 μl min−1.

Impact of connected conduit on pumping-induced seawater intrusion in a coastal karst aquifer

IntroductionCoastal karst aquifers face the risk of seawater intrusion due to groundwater development. Based on the conceptualization of Woodville Karst Plain (WKP), this study investigates the effect of karst conduit and pumping conditions on the aquifer vulnerability and pumping security.MethodsAccording to the purposes of this study, two cases are considered: one with conduit and one without. Salinization levels are compared between two cases, considering pumping rates ranging from 50 to 200 m3/day and various pumping locations throughout the on-shore region.ResultsThe results reveal that the aquifer with conduit is more susceptible to seawater intrusion at low pumping rates, whereas both scenarios experience significant salinization at high pumping rates. Specifically, in the non-conduit case, contamination is most prevalent when wells are located in the middle of the aquifer, while in the conduit case, pumping from inland areas poses high vulnerability. Moreover, conduit case and non-conduit case display different regions for pumping clean water. At low pumping rates, both cases show saline water being pumped from wells near the shore, and clean water is obtained from inland wells. At high pumping rates, the non-conduit case allows for clean water extraction from wells situated further inland, while in the conduit case, no wells within the entire aquifer are deemed safe.DiscussionThe analysis and findings of this study offer valuable insights for the management of groundwater in coastal karst aquifers, encompassing vulnerability assessment, selection of pumping locations, and determination of pumping rates.

Underbalanced Coiled Tubing Drilling: Delivering Well Production Safely in High H2S and Tight Gas Reservoirs, UAE

Summary United Arab Emirates (UAE) is seeking to become self-sufficient in gas supply by 2030. This has led the country to initiate several exploratory and appraisal projects to achieve this goal. This study covers one such pilot project targeting production from tight gas reservoirs in three wells through a coiled-tubing (CT) underbalanced drilling (UBD) project in ADNOC Onshore. CT pressure control equipment (PCE) was rigged up on top of production trees with wells already completed and cemented. A CT tower was used to accommodate the drilling bottomhole assembly (BHA) and eliminate risks related to its deployment. CT strings were designed to reach target intervals with sufficient weight on bit (WOB), suitable for sour environments, and able to withstand high pumping rates with mild circulating pressures. To address the hazards of H2S handling at the surface, a custom-fit closed-loop system was deployed. The recovered water was treated on the surface and reused for drilling to decrease the water consumption throughout the operations. The plan was to drill 3 3/4-in. horizontal laterals in all candidate wells. Each well was completed with a combination of a 4 1/2-in. and a 5 1/2-in. tubing and a 7-in. liner. Five laterals were drilled across the three candidate wells targeting carbonate reservoirs with each lateral having an average length of ~4,000 ft. The achieved rates of penetration varied significantly from 15 ft/min to 30 ft/min while drilling through the various formations. Over the course of the pilot project, several challenges had to be addressed, such as material accretion on the CT string during wiper trips, treatment of return fluids having high H2S content and rock cuttings, and ensuring the integrity of the CT pipe while operating in severe downhole environments. Solutions and lessons learned from each well were implemented subsequently in the campaign, such as the use of increased concentrations of H2S inhibitor to coat the CT string, the use of nitrified fluids based on changing well parameters to maintain underbalance, thorough pipe management through real-time CT inspection, and adding a fixed quantity of fresh water to the drilling system every day to avoid chemical reactions between the drilling fluid additives and hydrocarbons. The wells completed with this method exceeded production expectations by 35–50% across the project while reconfirming the value of the technology. The use of CT for UBD is still considered a challenging intervention worldwide. Such cases in high H2S environments are rare. This study outlines best practices for a CT UBD and a setup that can be replicated in other locations to implement this methodology with high H2S and when rig sourcing is a concern.

Field Experiments on 3D Groundwater Flow Patterns in the Deep Excavation of Gravel-Confined Aquifers in Ancient Riverbed Areas

In ancient riverbed areas, the hydro-geological conditions are extremely complex because of the cutting of ancient river channels during the sedimentary process. How to lower groundwater level in water-riched gravel-confined aquifer during deep excavation is vital for underground engineering. Groundwater flow patterns had to be understood during foundation pit dewatering. This paper presents a field case study conducted at the deep foundation pit of the Qianjiang Century City station on Hangzhou Metro Line 6, which is notable for its 52 m deep unclosed waterproof curtain. A total of 34 pumping wells were installed within the pit. During the tests, one well was subjected to a pumping well, while the others served as observation wells. The research included two sets of multi-depth pumping tests, which differed in terms of their filter lengths, aimed at investigating the flow pattern around pump wells and the roots of diaphragm walls. The study found that the use of longer filters, higher pump rates, and filters placed nearer to aquifer roofs enhances dewatering efficiency and minimizes impact on the surrounding geological environment. This paper introduces a novel concept known as the diaphragm wall–pumping well effect, which regulates the water head outside the pit and the subsidence, thereby optimizing the drawdown of the deep foundation pit with an unclosed waterproof curtain. The findings were applied in the foundation pit dewatering of Qianjiang Century City station, and the drawdown in and outside the pit was effectively controlled.

Evaluation of recharge estimation methods applied to fissure and karst aquifers of the Lagoa Santa Karst Environmental Protection Area, Brazil

AbstractThe Lagoa Santa Karst Environmental Protection Area is known worldwide as the place where the oldest human fossil in the Americas was found. Beyond its archaeological richness is speleological, faunistic and floristic richness across this remarkable karstic landscape. It is also a region with important aquifers for public and private water supply. Located in the state of Minas Gerais, Brazil, despite its environmental relevance, the area is characterized by increased anthropic environmental impacts. Regarding the relationship between surface water and groundwater, in order to investigate the annual input of water into the aquifers, this study aimed to estimate the annual recharge rate in the hydrological years 2017 to 2021 using three methods: the recession curve displacement method of Rorabaugh (International Association of Scientific Hydrology, 1964, 63:432–441), the recursive digital filter method by Lyne and Hollick (Institute of Engineers Australia National Conference, 1979, pp. 89–93), and the method based on water‐table fluctuation in tubular wells proposed by Healy and Cook (Hydrogeology Journal, 2002, 10(1):91–109). The average percentage of rainfall that is recharged into the aquifer, considering the overall period studied from 2017 to 2021, found with the three methods was 13%, 9% and 9%, respectively, which indicates that recharge rates within the study area are low and that higher values are observed within karst basins in comparison with basins located in pelitic zones. Overall, the methods yielded coherent values among each other and were compared with previous studies in the area. In relation to the automatized recursive digital filter method, the automatized recession curve displacement method was more adherent because it considers daily streamflow measurements. The WTF method was handy for the local recharge rate analysis and enabled an evaluation of recharge rates in each aquifer unit. Despite the similarity of the values obtained in the methods, they are likely underestimated due to the high pumping rates of groundwater. Furthermore, it is recommended to deepen the correlation between lithotype, karst geomorphology, and its dissolution processes to improve the understanding of recharge processes.

Experimental study on hydraulic fracture propagation behavior of horizontal well on multilayered rock

A series of true axial hydraulic fracturing experiments were conducted to understand the complex hydraulic fracture initiation and propagation behavior of multilayered rocks. Moreover, a new and convenient grid measurement method was presented to describe hydraulic fracturing effectiveness. The experimental results revealed that transverse vertical and non-planar axial fractures could be created in the hydraulic fracturing of multilayered rocks. Moreover, the fracture area scanning results by the grid measurement method match the results indicated by the traditional Geomagic method. The fracture behavior close to the layer contact determines how complex the produced fishbone structure fracture system is. Near the hole zones, secondary axial and deviated transverse fractures were found due to multiple layers. The fracture system can be managed by modifying the fracturing treatment parameters. Low-principle horizontal stress contrast and low-viscosity fracturing fluid can produce complicated fractures, and an increase in perforation numbers can make the fracture more complex and produce abnormally high breakdown pressure. Larger fracture areas benefit from high perforation rates and pump rates, but larger fracture areas may not always benefit from a decreased fracturing fluid viscosity. The breakdown pressure was the highest for the samples with two sets of oriented perforations, and the stress shadowing effect should be considered in the multiple fracture treatment. The increase in the pump rate generates a more complex fracture path in the horizontal well despite the fracturing fluid types but also yields a high breakdown pressure. The increased fracturing fluid viscosity can constrain the random fracture extension, which is beneficial for decreasing the near-wellbore tortuosity. The occurrence of shear fracture along the interface in a multilayered formation could be a risk for proppant injection and placement.

Numerical and Experimental Simulation of Hydraulic Fracture Propagation Mechanism in Conglomerate Formation Based on Hybrid Finite-Discrete Element Method

Hydraulic fracturing was the main technology to achieve the economic development of conglomerate reservoirs, knowing that the hydraulic fracture propagation mode was of great significance for improving the development of conglomerate reservoirs. This paper proposed a new method to understand the hydraulic fracture behavior based on a hybrid finite-discrete element method. The simulation indicated that a complex fracture network was created near the wellbore in the studied conglomerate reservoir, and hydraulic fracture propagation around the gravel layer was the main failure mode when the hydraulic fracture reached the gravel layer. From the simulations, it was shown that under small differences in horizontal stress and tensile strength, the hydraulic fracture propagated more easily around the gravel layer, while it could cross the gravel under large differences in horizontal stress and tensile strength. Greater tensile strength differences can reduce the complexity of the fracture network. In addition, higher pumping rates and viscosities of fracturing fluid contribute to the complex fracture network and also can produce more gravel crosses when the hydraulic fracture is met. The main reason was that a higher pumping rate and higher viscosity of fracturing fluid can obtain a higher net pressure, which can ensure the hydraulic fracture crosses the gravel layer.

In-situ measurement of the electron spin polarization by controlling its distribution in atomic ensembles.

The determination of electron spin polarization by controlling the atomic population distributions of ground states has been proposed. The polarization could be deduced by generating different population symmetries by polarized lights. The polarization of the atomic ensembles was decoded from optical depth in different transmissions of linearly and elliptic polarized lights. The feasibility of the method has been validated theoretically and experimentally. Moreover, the influences of relaxation and magnetic fields are analyzed. The transparency induced by high pump rates are investigated experimentally, and the influences of ellipticity of lights are also discussed. The in-situ polarization measurement was achieved without changing optical path of atomic magnetometer, which provides a new way to interrogate the performance of atomic magnetometer and in-situ monitoring the hyperpolarization of nuclear spins for atomic co-magnetometer.

Experimental study on hydraulic fracture propagation in heterogeneous glutenite rock

To understand the fracture behavior and discuss the possibility of the reservoir-stimulated volume concept on glutenite rock, true triaxial hydraulic fracturing experiments were performed on glutenite rocks with acoustic emission monitoring. Experimental results indicate that a curved hydraulic fracture (HF) can be created. In addition, in situ stress plays the greatest role in HF propagation direction regardless of gravel characteristics; however, the gravel with high mechanical strength could affect hydraulic fracture path locally. Intense fluctuate extension pressure and discrete surged acoustic emission (AE) events not only occur during the breakdown stage but also during the shut-in stage, indicating embedded gravels tend to influence HF process. The breakdown pressure is the highest for sample with low horizontal stress difference, while breakdown pressure for large-size sample with small gravel numbers is the lowest. The highest AE amplitude can be observed for sample where fracture positions on gravels are at the initial stage, while continuous high AE amplitude is observed for samples with high pump rates during the entire process, indicating intense fracture and gravel interactions. Moreover, four possible interaction behaviors between HF and gravels, namely, fracture penetration, deflection, diversion and arrest, can be identified near wellbore and far-field failure zones. In general, fracture penetration behavior is exclusively observed within the near wellbore failure zone. Additionally, the fracture width for a penetration fracture is the largest due to high fracturing energy, while fracture diversion and arrest exhibit the smallest fracture width. Given the existence of gravels, branch fractures are difficult to merge with residual fracturing energy. An extremely coarse HF surface and tortuous HF path can be found for all samples; thus, the selection of a suitable pump rate and fracturing fluid is necessary to migrate near wellbore tortuosity.

Studies on the Numerical Control Programming for Multi-Axis Machining of Turbomolecular Pump Rotor

Turbomolecular pumps (TMPs), boasting advantageous high pumping rates, stability, and cleanliness, have been widely used in the semiconductor and photoelectric industries. In the aviation industry, the lightweight rotors of turbomolecular pumps can enhance the performance of generators. With technological advancements and increased industrial performance demands, various designs for turbomolecular pump rotors utilizing twisted and curved blade surfaces have been proposed. This increase in complexity runs parallel with machining difficulties. Contact and noncontact reverse engineering equipment was used to reconstruct a computer-aided design (CAD) model of turbomolecular pump rotors. The machining of thin and long blades, cutting tool arrangement, and toolpath was planned. Postprocessing was used to convert the toolpath into numerical control (NC) programming codes, which were combined with solid model cutting simulation software to verify the efficacy of the generated machining NC program for turbomolecular pump rotors. A five-axis horizontal machining center (CK type) with aluminum alloy AL6061-T6 was used to conduct actual machining tests measuring the efficiency of the machining methods. The rapid prototyping (RP) blocks can be creatively used as a jig and stuffed between the blades to suppress the chatter problem during processing, and the roughness of the surface of the blades can be reduced from 4.4 μm to 1.3 μm. The processed rotor can meet the flow test requirements, and the overall research can be used as a reference for the industry.

Experimental and 3D Numerical Investigation on Proppant Distribution in a Perforation Cluster Involving the Artificial Neural Network Prediction

Summary Uniform proppant distribution in a cluster and a stage with multiple clusters is a primary objective to optimize fracturing parameters and improve the production from each cluster. Because fracturing slurry is typically pumped at high pressure and rate in fields, it is a big challenge to study proppant transport behavior and distribution characteristics through laboratory experiments. There is still a lack of an effective model to quantitatively evaluate proppant distribution based on an actual wellbore configuration. The objective is to propose a novel method to accurately evaluate the distribution uniformity and quickly optimize fracturing parameters based on field conditions. This paper conducts particle transport experiments in a horizontal pipe with six holes at the helical distribution. A 3D numerical model coupling of the computational fluid dynamics (CFD) and discrete element method (DEM) is used to study proppant distribution. Proppant distribution is quantitatively evaluated by the proppant transport efficiency (E) and normalized standard deviation (NSD). The effects of 10 parameters are investigated. An artificial neural network (ANN) model is developed to predict proppant distribution in a cluster. The results identify that proppant distribution among perforations is generally toe-biased in a horizontal wellbore due to a high pumping rate. Proppants with large inertia easily miss the heel-side holes and are suspended to the toe side. The complex vorticity flow carries them to the toe-side perforation regardless of hole orientation. Fluid distribution can significantly change proppant distribution regardless of fluid velocity. The heel-biased fluid distribution leads to the same bias of proppant, and the downward perforations receive more proppants. Proppant transport reaches equilibrium quickly, and the distribution is hard to change unless the injection condition varies. It is a good choice to increase fluid viscosity, add perforation sealers, and inject small mesh proppant, especially for the low density. The ANN model trained by extensive experimental and numerical samples can accurately evaluate proppant distribution uniformity. The study provides an efficient way to optimize injection parameter design and achieve real-time optimization coupled with the fiber-optic downhole diagnostic. It can be a crucial part of artificial intelligence hydraulic fracturing.

Human butyrylcholinesterase in Cohn fraction IV-4 purified in a single chromatography step on Hupresin.

Protection from the toxicity of nerve agents is achieved by pretreatment with human butyrylcholinesterase (BChE). Current methods for purifying large quantities of BChE from frozen Cohn fraction IV-4 produce 99% pure enzyme, but the yield is low (21%). Our goal was to simplify the purification procedure and increase the yield. Butyrylcholinesterase was extracted from frozen Cohn fraction IV-4 in 10 volumes of water pH 6. The filtered extract was pumped onto a Hupresin affinity column. The previously utilized anion exchange chromatography step was omitted. Solvent and detergent reagents used to inactivate lipid enveloped virus, bacteria and protozoa did not bind to Hupresin. BChE was eluted with 0.1 M tetramethylammonium bromide in 20 mM sodium phosphate pH 8.0. BChE protein was concentrated on a Pellicon tangential flow filtration system and demonstrated to be highly purified by mass spectrometry. A high pump rate produced protein aggregates, but a low pump rate caused minimal turbidity. Possible contamination by prekallikrein and prekallikrein activator was examined by LC-MS/MS and by a chromogenic substrate assay for kallikrein activity. Prekallikrein and kallikrein were not detected by mass spectrometry in the 99% pure BChE. The chromogenic assay indicated kallikrein activity was less than 9 mU/mL. This new, 1-step chromatography protocol on Hupresin increased the yield of butyrylcholinesterase by 200%. The new method significantly reduces production costs by optimizing yield of 99% pure butyrylcholinesterase.

Watt-Level High-Efficiency Deep-Red Ho3+:ZBLAN Fiber Laser

To date, Ho <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> -doped fluoride fibers have been investigated as a viable route for deep-red fiber lasers. However, high output performance deep-red fiber lasers around 750 nm have been proven challenging to achieve due to limitations in both the excitation source and pumping mechanism. In this study, we use a home-made 640 nm pump source to develop a deep-red laser with a high pumping rate, and obtain a compact watt-level high efficiency deep-red laser output. Population inversion is achieved by strong excited state absorption in a Ho <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> -doped ZBLAN fiber. We realize a maximum 1.46 W deep-red fiber laser operating at 752.6 nm, and its slope efficiency is as high as 53%. The improved rate equations are used to describe the quasi-four-level system, and the experimental results agree well with the simulations. Moreover, with the development of blazed grating, we demonstrate a tunable deep-red fiber laser with a tuning range of 13.7 nm, from 746.5 to 760.2 nm, which is the widest tuning range of Ho <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> -doped fiber laser at deep-red wavelengths.