Accurate wind speed measurement in low-pressure conditions is crucial for the thermal performance validation and attitude control of stratospheric aircraft. As air density decreases, traditional wind speed measurement systems based on principles such as dynamic pressure, heat transfer, ultrasound, and particle velocimetry face significant challenges when applied in low-pressure environments, often failing to achieve the required measurement accuracy. This paper presents the development of a wind speed simulation system based on a rotation method designed to operate in low-pressure conditions, utilizing a space environment simulation chamber in conjunction with a high-precision turntable. The system was employed to conduct response tests on a constant heat flow thermal sphere anemometer within a stratospheric pressure range of 1 kPa to 30 kPa. The experimental results revealed that at extremely low Reynolds numbers, the probe signal exhibited increasing nonlinearity, significantly affecting the response curve at pressures below 15 kPa. While the sensitivity of the hot-sphere probe remained relatively stable at wind speeds above 5 m/s, it decreased nonlinearly as the pressure dropped when wind speeds fell below 5 m/s. Furthermore, this paper analyzes the impact of various interpolation methods on wind speed conversion errors, providing valuable data to support the future development and validation of stratospheric aircraft.