High-performance Electrochemical Biosensors Research Articles

Molecularly imprinted electrochemical biosensor for thrombin detection by comparing different monomers.

Aim: Investigating molecularly imprinted polymers (MIPs) in electrochemical biosensors for thrombin detection, an essential protein biomarker. Comparing different monomers to showcase distinct sensitivity, specificity and stability advantages. Materials & methods: Dopamine, thionine and ethanolamine serve as monomers for MIP synthesis. Electrochemical methods and atomic force microscopy characterize sensor surfaces. Performance is evaluated, emphasizing monomer-specific electrochemical responses. Results: Monomer-specific electrochemical responses highlight dopamine's superior signal change and stability over 30days. Notably, a low 5pg/ml limit of detection, a broad linear range (5-200pg/ml) and enhanced selectivity against interferents are observed. Conclusion: Dopamine-based MIPs show promise for high-performance electrochemical thrombin biosensors, suggesting significant applications in clinical diagnostics.

In Situ Exfoliation Growth Strategy Realizing Controlled Synthesis of 3D to 2D MOF Materials as High-Performance Electrochemical Biosensors.

Two-dimensional (2D) metal-organic framework (MOF) nanosheets with large surface area, ultrathin thickness, and highly accessible active sites have attracted great research attention. Developing efficient approaches to realize the controllable synthesis of well-defined 2D MOFs with a specific composition and morphology is critical. However, it is still a significant challenge to construct thin and uniform 2D MOF nanosheets and resolve the reagglomeration as well as poor stability of target 2D MOF products. Here, an "in situ exfoliation growth" strategy is proposed, where a one-step synthetic process can realize the successful fabrication of PBA/MIL-53(NiFe)/NF nanosheets on the surface of nickel foam (NF) via in situ conversion and exfoliation growth strategies. The PBA/MIL-53(NiFe)/NF nanosheets combine the individual advantages of MOFs, Prussian blue analogues (PBAs), and 2D materials. As expected, the resulting PBA/MIL-53(NiFe)/NF as a glucose electrode exhibits an extremely high sensitivity of 25.74 mA mM-1 cm-2 in a very wide concentration range of 180 nM to 4.8 μM. The present exciting work provides a simple and effective strategy for the construction of high-performance nonenzymatic glucose electrochemical biosensors.

Non-enzymatic electrochemical sensor based on ZnO nanoparticles/porous graphene for the detection of hypoxanthine in pork meat

In this study, we developed a pioneering non-enzymatic electrochemical sensor utilizing a flexible porous graphene electrode modified with ZnO nanoparticles (ZnO/fPGE sensor) to assess hypoxanthine (HXA) content in pork at post-mortem time. The ZnO/fPGE sensor was synthesized via hydrothermal method and direct laser writing with a CO2 laser on a polyimide film at ambient conditions. Its characterization was analyzed by Raman, Fourier-transform infrared spectroscopy, field-emission scanning microscopy, energy-dispersive x-ray spectroscopy, and cyclic voltammetric techniques. Linear response, the limit of detection, and sensitivity to the HXA were enhanced with the values of the range from 1.5 to 150, 0.14 µM, and 6.6 µA μM−1 cm−2, respectively. Effective resistance to common physiological interferences (such as uric acid, ascorbic acid, dopamine, glucose, and xanthine) was indicated, and additionally, the determination of HXA concentration in real samples with good selectivity is attributed to the synergistic effects between ZnO nanoparticles and fPGE. Therefore, the ZnO/fPGE has provided a favorable electrical environment for developing high-performance electrochemical biosensors to determine hypoxanthine in pork meat.

A high-performance electrochemical biosensor using an engineered urate oxidase.

We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM-1 mM), and more than 28 days lifetime.

Peptide Nanosheet-Inspired Biomimetic Synthesis of CuS Nanoparticles on Ti3C2 Nanosheets for Electrochemical Biosensing of Hydrogen Peroxide.

Hydrogen peroxide (H2O2) is one of the intermediates or final products of biological metabolism and participates in many important biological processes of life activities. The detection of H2O2 is of great significance in clinical disease monitoring, environmental protection, and bioanalysis. In this study, Ti3C2-based nanohybrids are prepared by the biological modification and self-assembled peptide nanosheets (PNSs)-based biomimetic synthesis of copper sulfide nanoparticles (CuS NPs), which show potential application in the fabrication of low-cost and high-performance electrochemical H2O2 biosensors. The synthesized CuS-PNSs/Ti3C2 nanohybrids exhibit excellent electrochemical performance towards H2O2, in which CuS NPs can catalyze the decomposition of H2O2 and realize the transformation from a chemical signal to an electrical signal to achieve the purpose of H2O2 detection. The prepared CuS-PNSs/Ti3C2-based electrochemical biosensor platform exhibits a wide detection range (5 μM-15 mM) and a low detection limit (0.226 μM). In addition, it reveals good selectivity and stability and can realize the monitoring of H2O2 in a complex environment. The successful biomimetic synthesis of CuS-PNSs/Ti3C2 hybrid nanomaterials provides a green and friendly strategy for the design and synthesis of functional nanomaterials and also provides a new inspiration for the construction of highly effective electrochemical biosensors for practical detection of H2O2 in various environments.

A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1.

High-performance electrochemical biosensors for the rapid detection of aflatoxin B1 (AFB1) are urgently required in the food industry. Herein, a multi-scaled electrochemical biosensor was fabricated by assembling carboxylated polystyrene nanospheres, an aptamer and horseradish peroxidase into a free-standing carbon nanofiber/carbon felt support. The resulting electrochemical biosensor possessed an exceptional performance, owing to the unique structures as well as the synergistic effects of the components. The 3D porous carbon nanofiber/carbon felt support served as an ideal substrate, owing to the excellent conductivity and facile diffusion of the reactants. The integration of carboxylated polystyrene nanospheres with horseradish peroxidase was employed as a signal amplification probe to enhance the electrochemical responses via catalyzing the decomposition of hydrogen peroxide. With the aid of the aptamer, the prepared sensors could quantitatively detect AFB1 in wine and soy sauce samples via differential pulse voltammetry. The recovery rates of AFB1 in the samples were between 87.53% and 106.71%. The limit of detection of the biosensors was 0.016 pg mL−1. The electrochemical biosensors also had excellent sensitivity, reproducibility, specificity and stability. The synthetic strategy reported in this work could pave a new route to fabricate high-performance electrochemical biosensors for the detection of mycotoxins.

Nanomaterial-based electrochemical enzymatic biosensors for recognizing phenolic compounds in aqueous effluents

With the rapid development of industrial society, phenolic pollutants already identified in water are severe threats to human health. Traditional detection techniques like chromatography are poor in the ability of cost-effectiveness and on-site detection. In recent years, electrochemical enzymatic biosensors have attracted increasing attention for use in the recognition of phenolic compounds, which is considered an effective strategy for the product transfer of portable analytical devices. Although electrochemical enzymatic biosensors provide a fast, accurate on-site detection technique, the difficulties of enzyme deactivation, poor stability and low sensitivity remain to be solved. Thus, effective immobilization methods of enzymes and nanomaterials with excellent properties have been extensively researched to obtain a high-sensitivity and high-stability biosensing platform. Simultaneous detection of multiple phenols may become the focus of further research. In this review, we provide an overview of recent progress toward electrochemical enzymatic biosensors for the detection of phenolic compounds, including enzyme immobilization approaches and advanced nanomaterials, especially nanocomposites with attractive properties such as good conductivity, high specific surface area, and porous structure. We will comprehensively discuss the features and mechanisms of the main enzymes adopted in the construction of different phenolic biosensors, as well as traditional methods (e.g., adsorption, covalent bonding, entrapment, encapsulation, cross-linking) of enzyme immobilization. The most effective method is based on the properties of enzymes, supports and application objective because there is no one-size-fits-all method of enzymatic immobilization. The emphasis will be given to various advanced nanomaterials, including their special nanostructures, preparation methods and performance. Finally, the main challenges in future research on electrochemical phenolic biosensors will be discussed to provide further perspectives for practical applications in dynamic and on-site monitoring. We believe this review will deliver an important inspiration for the construction of novel and high-performance electrochemical biosensors from enzyme selection to nanomaterial design for the detection of various hazardous materials. We believe this review will deliver an important inspiration on the construction of novel and high-performance electrochemical biosensors from the enzyme selection to the nanomaterial design for detections of various hazardous materials.

Dual nanozyme based on ultrathin 2D conductive MOF nanosheets intergraded with gold nanoparticles for electrochemical biosensing of H2O2 in cancer cells

The development of facile, rapid and cost-effective strategies for sensitive detection of cancer biomarkers in human samples is of great significance for early diagnosis of malignant tumors related diseases. In this work, we develop a high-performance electrochemical biosensor based on highly active dual nanozyme amplified system, i.e., ultrathin two-dimension (2D) conductive metal–organic framework (C-MOF) nanosheets (NSs) decorated with high-density ultrafine gold nanoparticles (Au-NPs), and explore its application in sensitive detection of cancer biomarker H2O2 in live cells. The C-MOF NSs {i.e., Cu-HHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene)-NSs} provide large surface area and abundant active open metal sites (Cu–O4), which could improve the catalytic activity of Cu-HHTP-NSs towards H2O2. Moreover, abundant exposed O atoms also serve as anchor sites for the deposition of high-density ultrafine Au-NPs (∼3 nm) without agglomeration. Owing to the synergistic contributions of high catalytic activity of Cu-HHTP-NSs and Au-NPs as well as their unique structural and electrical properties, the as-prepared nanohybrid modified electrode exhibits good sensing performances to H2O2 with an extremely low detection limit of 5.6 nM (3σ rules) and a high sensitivity of 188.1 μA cm−2 mM−1. Furthermore, the proposed nanozymatic electrochemical biosensor has been applied in real-time tracking H2O2 released from different human colon cells to identify colon cancer cells from normal colon epithelial cell, which demonstrates its great prospect for early diagnosis and management of various cancer diseases.

Metal Nanocomposites Based Electrochemical Sensor Platform for Few Emerging Biomarkers

:Design of high-performance electrochemical sensor and biosensor platforms has attracted much devotion for the sensitive and selective detection of emergent biomarkers. Electrochemical sensor offers numerous advantageous features include cost-effective and ease of miniaturization, rapid and online monitoring, simultaneous detection ability, etc., which have captivated the potential interdisciplinary research. In this review, the advances and challenges towards the electrochemical detection of emergent biomarkers such as hydrogen peroxide, nitric oxide, β-nicotinamide adenine acetaminophen, dinucleotide (NADH) in biofluids are highlighted based on the recent research outcomes. In fact, the effective utility and benefits of transition metal nanocomposites without the utilization of biological materials, including enzymes, antibodies, etc. as electrode materials towards the detection of selected biomarkers in practical biofluids, monitoring early-stage and diagnosis of disease related biomarkers, are also described. These promising metal nanomaterials based electrochemical sensor platforms concrete the tactic for a new generation of sensing devices.

Tamarindus indica seed-shell nanoparticles‑silver nanoparticles-Ceratonia silique bean gum composite for copper-micro mesh grid electrode fabrication and its application for glucose detection in artificial salivary samples

This study used a new approach to fabricate a glucose detection system based on nano-engineered biomaterials. The fabrication steps included strategic synthesis, integration and stabilization of biological and metal nanoparticles in superabsorbent hydrogel gum matrix. The design of the high-performance electrochemical biosensor platform includes copper-micro mesh grid electrode modified with polymer phase comprising of silver nanoparticles surface coroneted with Ceratonia silique locust bean gum (LBG), Tamarindus indica seed-shell nanoparticles and glucose oxidase (GOx). Fundamental assessment of catalytic properties of the nanobiocomposite films on copper grid probe were performed by cyclic voltammetry, amperometry, differential pulse voltammetry. Probes showed good repeatability, reproducibility, selectivity, and long-term stability. The GOx was well-immobilized and stabilized by C. siliqua nano-matrix, with 85% and 98% activity retention when stored at different condiions for 6 month and 3 months, respectively. The fabricated grid-platform exhibited linear response in a wide range of glucose concentration, with detection limit of 1.0 nM (S/N = 3) and sensitivity 38.7 mA nM−1 cm−2. The bionanomaterial-based sensor was successfully applied for ultra-low glucose detection in artificial salivary samples. The designed sensor, perhaps with further modifications, has potential for the next generation of sensing platform in various biological fluids especially for non-invasive glucose detection for diabetic patients.

Electrochemical chloramphenicol sensors-based on trace MoS2 modified carbon nanomaterials: Insight into carbon supports

Carbon nanomaterials are considered as potentially promising sensing materials for fabrication of high-performance electrochemical chloramphenicol (CAP) sensors. However, it is highly required to investigate the structure effect of carbon supports on electrochemical sensing performances. Herein, three typical carbon nanomaterials were selected as carbon supports to deposition of trace MoS2, including reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs) and carbon black (CB), which were subsequently used to construct electrochemical CAP sensors. The combined characterizations of Raman spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and transmission electron microscopy indicate that these MoS2 modified carbon nanomaterials show the morphology and microstructures similar with carbon supports as used. Electrochemical tests indicate that the sensing performances of these CAP sensors are dependent on the structure of carbon supports. Among all these MoS2 modified carbon nanomaterials, MoS2 modified rGO hybrids display large electrochemically active surface area and low interfacial resistance between electrolyte and electrode, resulting in the highest sensitivity of 3.581 μA μM−1 cm−2. As a result, rGO is regarded as good candidate for fabrication of high-performance CAP sensors, compared to MWCNTs and CB. The present work not only clarifies structure effect of carbon supports on electrochemical sensing performances, but also provides help for the construction of high-performance electrochemical sensors and biosensors based on carbon nanomaterials.

A Nanometer-Sized Graphite/Boron-Doped Diamond Electrochemical Sensor for Sensitive Detection of Acetaminophen.

A boron-doped diamond (BDD) has been widely used as an outstanding electrode for constructing high-performance electrochemical biosensors. In this paper, we fabricated a novel electrode combined of nanometer-sized graphite-BDD film (NG-BDD) by chemical vapor deposition. The nanometer-sized graphite (NG) is formed on the (111) facet of BDD via converting an sp3 diamond structure to an sp2 graphitic phase at high temperature in boron-rich ambient. The electrode was characterized by means of scanning electron microscopy, Raman spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. This NG-BDD was performed as an electrode of electrochemical biosensor to detect trace acetaminophen (APAP) accurately. Cyclic voltammetry and differential normal pulse voltammetry are used to investigate the overall performance of the electrochemical device. The sensor has a linear electrochemical response to APAP in the concentration range of 0.02–50 μM, and the detection limit is estimated to be as low as 5 nM. The research has resulted in a solution of constructing a reusable NG-BDD sensor to detect APAP with stability and show potential in extensive application.

Magnetic-Assisted Methylene Blue-Intercalated Amplified dsDNA for Polarity-Switching-Mode Photoelectrochemical Aptasensing.

Organic dyes are typically applied as photosensitizers in photoelectrochemical (PEC) cells but have not been reported in polarity-reversal-mode PEC sensors with excellent sensitivity and accuracy. Herein, an elegant and robust PEC biosensor for carcinoembryonic antigen (CEA) has been designed by photocurrent polarity switching of CdTe quantum dots (QDs), which is obtained by embedding methylene blue (MB) into amplified double-stranded DNA (dsDNA) anchored to the superparamagnetic Fe3O4@SiO2. The target-triggered Exo III-assisted cyclic amplification strategy and in situ magnetic enrichment enable the remarkable sensitivity. The extraction of target-analogue single-stranded DNA (output DNA) contributes to high selectivity resulting from the elimination of possible interferences in real samples or matrixes. Particularly, this exclusive polarity-reversal-mode PEC aptasensing can efficiently eliminate the false-positive or false-negative signals, leading to accurate measurements. Moreover, different from the probes and layer-by-layer assembled photoelectric beacons on electrodes in advance, this rational split-type approach is doomed to help the PEC biosensor with additional merits of convenient fabrication, short time consumption, wider linearity, as well as outstanding reproducibility and stability in practical applications. In light of the ability of MB acting as a kind of signal probe in typical electrochemical sensors, certainly, this ingenious design can not only be extended to a wide variety of target monitoring but also provide new ideas for the construction of high-performance electrochemical and PEC biosensors.

Nanomaterial-based electrochemical sensors and biosensors for the detection of pharmaceutical compounds

The surging growth of the pharmaceutical industry is a result of the rapidly increasing human population, which has inevitably led to new biomedical and environmental issues. Aside from the quality control of pharmaceutical production and drug delivery, there is an urgent need for precise, sensitive, portable, and cost-effective technologies to track patient overdosing and to monitor ambient water sources and wastewater for pharmaceutical pollutants. The development of advanced nanomaterial-based electrochemical sensors and biosensors for the detection of pharmaceutical compounds has garnered immense attention due to their advantages, such as high sensitivity and selectivity, real-time monitoring, and ease of use. This review article surveys state-of-the-art nanomaterials-based electrochemical sensors and biosensors for the detection and quantification of six classes of significant pharmaceutical compounds, including anti-inflammatory, anti-depressant, anti-bacterial, anti-viral, anti-fungal, and anti-cancer drugs. Important factors such as sensor/analyte interactions, design rationale, fabrication, characterization, sensitivity, and selectivity are discussed. Strategies for the development of high-performance electrochemical sensors and biosensors tailored toward specific pharmaceuticals are highlighted to provide readers and scientists with an extensive toolbox for the detection of a wide range of pharmaceuticals. Our aims are two-fold: (i) to inspire readers by further elucidating the properties and functionalities of existing nanomaterials for the detection of pharmaceuticals; and (ii) to provide examples of the potential opportunities that these devices have for the advanced sensing of pharmaceutical compounds toward safeguarding human health and ecosystems on a global scale.

Hierarchically Porous Copolymer Film as Immobilization Matrix for Phenol Biosensor with High Sensitivity

A hierarchically porous structure of the matrix for immobilization of bioactive molecules is of crucial importance in improving the performance of electrochemical biosensors. In the present work, a block copolymer film was prepared via spinodal-decomposition-induced macro- and mesophase separation plus extraction by rinsing (SIM2PLE). The film exhibited an interconnected porous structure including macropores and mesopores. Thus, the specific surface area of the film was greatly enhanced, which was a benefit to the subsequent immobilization of enzyme. Then the resultant porous block copolymer film was adopted to construct a high-performance electrochemical biosensor to detect phenolic compounds. The as-developed phenolic biosensor exhibited a high sensitivity (3.0 × 103 mA·M–1·cm–2), quick response (<5 s), and extremely low detection limit (7 nM at an S/N ratio of 3). Moreover,a decent linear range (0.02–35 μM) was also observed. This work demonstrates that the hierarchically porous film based on copolymer...

(Invited) Design of Nanomaterial-Based Electrochemical Sensors for Environmental and Biological Applications

Electrochemical sensing strategies have spurred intense scientific interest owing to having the advantages of instrumental simplicity, rapid response, high sensitivity, cost-effectiveness, and portability. Nanomaterials and nanotechnology have facilitated the development of high-performance electrochemical sensors and biosensors with unique functionalities and processes for enhanced medical and environmental applications. Recently, my research team has designed and fabricated a variety of functional nanomaterials and nanocomposites. In this talk, the synthesis of advanced gold, metal oxides and graphene-based nanomaterials as well as their promising applications in electrochemical sensing will be presented. The electrochemical detection of biomarkers (e.g. nitric oxide), food additives (e.g. nitrite, sulfite, caffeic acid) and pollutants (e.g. mercury) will be discussed. The significant roles of nanomaterials in the development of high-performance electrochemical sensors will be highlighted.

A Novel Electrochemical Biosensor Based on Hematite (α-Fe2O3) Flowerlike Nanostructures for Sensitive Determination of Formaldehyde Adulteration in Fruit Juices

High-performance electrochemical enzymatic biosensor based on flowerlike α-Fe2O3 nanostructures was successfully developed for the detection of potential food adulterant, formaldehyde (formalin). The biosensor was found to be highly sensitive (744.15 μA mg−1 Lcm−2) with linear range of detection (0.01–0.3 mg/L) and showed high shelf-life (9 weeks) and precision (0.73% RSD) with reasonably good reproducibility. The biosensor application in real sample analysis was successfully accomplished using cyclic voltammetry (CV) technique. The developed biosensor exhibited detection limits of 0.02 mg/L and 0.04 mg/L in extracted and commercial orange juice samples, respectively, while 0.03 mg/L in extracted mango juice and 0.05 mg/L in commercial mango juice were obtained. The obtained detection limits are well below the maximum daily dose reference set by Environmental Protection Agency (EPA), USA, for formaldehyde. Biosensor results were found in good agreement with those obtained with HPLC (p < 0.05) and highlight market acceptability with usefulness and effectiveness of the proposed method for food quality and safety evaluation.

Rational Construction of 3D-Networked Carbon Nanowalls/Diamond Supporting CuO Architecture for High-Performance Electrochemical Biosensors.

Tremendous demands for highly sensitive and selective nonenzymatic electrochemical biosensors have motivated intensive research on advanced electrode materials with high electrocatalytic activity. Herein, the 3D-networked CuO@carbon nanowalls/diamond (C/D) architecture is rationally designed, and it demonstrates wide linear range (0.5 × 10-6 -4 × 10-3 m), high sensitivity (1650 µA cm-2 mm-1 ), and low detection limit (0.5 × 10-6 m), together with high selectivity, great long-term stability, and good reproducibility in glucose determination. The outstanding performance of the CuO@C/D electrode can be ascribed to the synergistic effect coming from high-electrocatalytic-activity CuO nanoparticles and 3D-networked conductive C/D film. The C/D film is composed of carbon nanowalls and diamond nanoplatelets; and owing to the large surface area, accessible open surfaces, and high electrical conduction, it works as an excellent transducer, greatly accelerating the mass- and charge-transport kinetics of electrocatalytic reaction on the CuO biorecognition element. Besides, the vertical aligned diamond nanoplatelet scaffolds could improve structural and mechanical stability of the designed electrode in long-term performance. The excellent CuO@C/D electrode promises potential application in practical glucose detection, and the strategy proposed here can also be extended to construct other biorecognition elements on the 3D-networked conductive C/D transducer for various high-performance nonenzymatic electrochemical biosensors.

Surface N-doped graphene sheets induced high electrocatalytic activity for selective ascorbic acid sensing

In this study, the graphene sheets embedded carbon (GSEC) film was modified with surface N-doping for highly selective ascorbic acid (AA) sensing. We found that the N-doped graphene sheets formed in-situ at the carbon film surface and induced high electrocatalytic activity for AA oxidation. Although surface N-doping slightly decreased the ID/IG and surface roughness of carbon film, the graphene sheets embedded in amorphous were preserved. With surface N-doping, the charge-transfer resistance was reduced from 22.5 Ω cm2 to 3.9 Ω cm2, the oxidation-reduction peak separation decreased to a low value of 65.7 mV in Fe(CN)64−/3− redox system, and the standard rate constant was increased from 0.24×10-2 to 1.36×10-2 cm·s-1. The surface N-doped graphene sheets embedded carbon (SN-GSEC) decreased the oxidation potential of AA from 0.186 V to 0.025 V (vs. Ag/AgCl) and realized selective detecting of AA. The mechanism may be that the surface N-doping induce the adjacent carbon in graphene sheets to obtain a higher partial density of states and more positive compensating charge. This study provides a simple method for preparing high performance electrochemical biosensor.

High Performance Electrochemical Biosensor for Bisphenol A Using Screen Printed Electrodes Modified with Multiwalled Carbon Nanotubes Functionalized with Silver-Doped Zinc Oxide

This study reports on a novel electrochemical biosensor for the detection of Bisphenol A (BPA) using a carbon-screen printed electrode modified with multiwalled carbon nanotubes that are functionalized with silver doped zinc oxide nanoparticles (Ag-ZnONPs) on which laccase enzyme was immobilized. The nanocomposite was characterized in stages by UV–visible, Fourier Transform Infrared Spectroscopy, scanning and transmission electron microscopes. The synthesized hybrid Ag-ZnONPs were spherical in shape with an average size of ~ 20 nm. The surface of Lac/Ag–ZnO/MWCNTs/C-SPE was also characterized using electrochemical methods. The presence of laccase on the surface of the nanocomposite-modified electrode reduced the charge transfer resistance of the redox couple and thereby improved the sensitivity towards the oxidation of Bisphenol A. The catalytic activity was further evaulated with 80 µM solution by cyclic voltammetry. Under optimum conditions, the biosensor displayed outstanding performance for BPA with a linear range 0.5–2.99 µM (R2 > 0.9943) and a limit of detection (LOD) of 6.0 nM. Furthermore, this proposed lacasse biosensor is more selective and stable with a high reproducible response factor (RSD of 0.86%), and was able to adequately quantify BPA in plastic bottle samples. In the light of our results, the biosensor showed promising and trustworthy analytical performance that is ideal for routine sensing applications for BPA in plastic bottles.