Higher Oxidative Stress Index Research Articles

Enhancement of oxidative stress, oxidative dna damage and lowered pefr in gasoline station attendants in calabar metropolis

Exposure to gasoline fumes through inhalation or accidental ingestion has been associated with chronic inflammatory reactions leading to oxidative stress, oxidative DNA damage and increased risk of chronic lung conditions and cancer. This study assessed the peak expiratory flow rate (PEFR) and biomarkers of oxidative stress (total antioxidant capacity (TAC), total plasma peroxides (TPP), oxidative stress index (OSI)), oxidative DNA damage (8-hydroxy-2-deoxyguanosine (8-OHdG)), 1-hydroxypyrene and urine creatinine in Gasoline Station Attendants. A total of 100 consenting adults, aged 18-60 years, comprising 50 gasoline station attendants and 50 non-gasoline station attendants (controls) were enrolled into this comparative cross-sectional study. The PEFR was determined using the peak flow meter, TAC, TPP and creatinine by colorimetry, 8-OHdG by enzyme linked immunosorbent assay (ELISA), 1-hydroxypyrene (1-HOP) by high performance liquid chromatography and OSI by calculation. Data was analysed using unpaired Student t-test and Pearson's correlation analysis at p<0.05.
 The body mass index, TPP, OSI and 8-OHdG levels were significantly higher and TAC and PEFR lower in gasoline pump attendants compared to the control groups (p<0.05). Positive correlations were observed between TPP and years at work (r=0.638, p=0.000), between TPP and OSI (r=0.282, p=0.047) and negative correlation between TAC and OSI (r=-0.555, p=0.000) only in gasoline station attendants. Exposure to gasoline is associated with increased lipid peroxidation and oxidative DNA damage, reduced lung function and depletion of antioxidants which may result in oxidative stress and increased risk for the development of chronic lung conditions in gasoline station attendants.

Comparison of Oxidative Stress Levels in Healthy Children and Children with Allergic Rhinitis.

Background/aim: Allergic rhinitis (AR) is characterized by chronic inflammation of the nasal mucosa. Under the influence of exogenous factors - allergens, reactive oxygen species (ROS) are released during cellular metabolism. They induce a series of pathological changes in the mucosa. Oxidative stress is а result of an imbalance between the production of ROS and the ability to neutralize them. The aim of this study is to compare the levels of oxidative stress between healthy children and children with allergic rhinitis. Material and methods: A total number of 60 children were included (30 healthy children and 30 children with AR). The oxidative stress index was determined by using the FRAS 5 (Free Radical Analytical System) Bravo system. Demographic characteristics, medical history, children's living conditions and eating habits were obtained from the questionnaire. Anthropometric measurements and the absolute number of eosinophils in the peripheral smear were performed on each child. Results: This study showed high oxidative stress index and a significantly higher value of the absolute number of eosinophils in the peripheral smear in children with AR in comparison to healthy children (p<0.05). The group of children with AR had more atopic characteristics and was more exposed to passive smoking than healthy children. Conclusion: Compared to healthy children, children with AR have a high index of oxidative stress, despite of the very high mean value of the concentration of water-soluble antioxidants in serum (PAT test) in the group of children with AR.

Association of Smoking with Total Oxidant and Antioxidant Levels in Breast Milk

Background: Breast milk is a natural food that contains all the fluids, energy, and nutrients necessary for the optimum growth and development of newborns. Smoking is a public health problem that has harmful effects on the mother and baby. This study aimed to examine the association of exposure to smoking with total oxidant status (TOS) and total antioxidant status (TAS) in breast milk. Methods: Healthy mothers without any health problems during their pregnancy and lactation periods were selected as subjects. Eighty-eight milk samples (44 in the smoking group and 44 in the nonsmoking group) were examined. TOS and TAS were analyzed using Rel Assay commercial kits. Results: TAS level was significantly lower in the smoking group than in the nonsmoking group (p < 0.05). TOS level was higher in the smoking group than in the nonsmoking group, although the difference was not statistically significant (p > 0.05). Oxidative stress index (OSI) was significantly higher in the smoking group than in the nonsmoking group (p < 0.05). Conclusions: Exposure to smoking was associated with low TAS and high OSI in breast milk.

Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses.

The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (n = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 °C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (p < 0.001) with those of low displaying higher resilience (p < 0.01). Boars were hierarchically clustered into two groups (p < 0.001) as having either positive or negative farrowing rate and litter size deviation; the negative one showing higher seminal OSI (p < 0.05). In sum, seminal OSI was negatively related to sperm motility and the in vivo fertility of liquid-stored boar semen AI-doses, with the receiver operating characteristic curve presenting seminal OSI as a good predictive biomarker of in vivo fertility of AI-boars (area under the curve: 0.815, p < 0.05).

The role of N-acetylcysteine in preventing hepatic injury associated with systemic oxidative stress after extracorporeal shock wave treatment.

Systemic oxidative stress may cause detrimental consequences for the liver, leading to hepatic fibrogenesis. To investigate histopathological changes in liver tissues due to the increased systemic oxidative stress associated with rat extracorporeal shock wave lithotripsy (SWL) model and to document the consequences of N-acetylcysteine (NAC) administration. In this experimental SWL model, 18 Wistar albino rats were randomly assigned into 3 groups. The control group (group I) had no intervention. Group II underwent SWL treatment with intraperitoneal saline injection. Group III also had SWL with intraperitoneal NAC and was divided into short-term (group III-14 days) and long-term (group III-28 days) subgroup. Hepatectomy was performed for histopathological examinations. Histopathological alterations were evaluated with light microscopy. Immunohistological staining for p53 and myeloperoxidase was also performed. Blood samples revealed a significant increase in plasma oxidative stress index (OSI) after plasma total antioxidant status (TAS) and total oxidant status (TOS) had been measured. It was shown that this increased systemic oxidative stress adversely affected liver tissues. Predominantly, sinusoidal dilatation was remarkably observed in rats with significantly high OSI values (p = 0.043). Similarly, periportal necrosis significantly increased in rats with high OSI values (p = 0.033). p53 positivity was also remarkable in rats with systemic oxidative stress (p = 0.049). N-acetylcysteine administration provided a significant decrease in OSI. N-acetylcysteine also improved all these alterations, including p53 staining. Particularly, sinusoidal dilatation was significantly protected in the long-term NAC group (group III-28 days). We demonstrated that SWL-induced systemic oxidative stress causes histological alterations in liver tissues. Increased p53 and myeloperoxidase staining as markers of oxidative damage were also detected. N-acetylcysteine may protect from these histological and ultra-structural alterations related to oxidative stress.

Investigation of the Role of Oxidative Stress and Factors Associated with Cardiac Allograft Vasculopathy in Patients after Heart Transplantation.

Oxidative stress is defined as an imbalance between the production of free radicals and their elimination by the antioxidant defense system. However, the role of oxidative stress in cardiac allograft vasculopathy (CAV) has not been fully understood. Therefore, this study is aimed at determining the role of oxidative-antioxidative balance disturbances in patients after HT. Furthermore, we sought to analyze factors associated with the presence of CAV, with particular emphasis placed on oxidative stress markers. The study analyzed data of 194 consecutive patients after HT who underwent routine visits in the Transplantation Clinic between 2015 and 2016. Total oxidant status (TOS) and total antioxidant capacity (TAC) were measured by the method described by Erel. The oxidative stress index (OSI) was defined as the ratio of the TOS to TAC levels. Patients' mean age was 55.4 ± 15.0 years, and 73.4% were men. The frequency of CAV was 50%. The area under the receiver operating characteristic curves indicated a good discriminatory power of TAC and TOS (0.8940 (0.8515-0.9365); 0.8620 (0.8126-0.9114), respectively) as well as excellent discriminatory power of OSI (0.9530 (0.9279-0.9781)) for CAV detection. Multivariate analysis of the Cox proportional hazard model confirmed that OSI (hazard ratio (HR) = 1.294 (1.204-1.391), p < 0.0001), age (HR = 1.023 (1.006-1.041), p = 0.0091), and high-sensitivity C-reactive protein (HR = 1.049 (1.016-1.083), p = 0.0151) were independently associated with CAV presence. In conclusion, TAC and TOS had a good discriminatory power and OSI had excellent strength for detecting CAV. The independent factors of CAV were higher OSI and CRP levels, as well as older recipient age.

Lactonase activity and status of paraoxonase 1 and oxidative stress in neonates of women with gestational diabetes mellitus.

The level and lactonase activity of paraoxonase 1 (PON1) and their association with PON1 genetic variants and oxidative stress are unclear in neonates of women with gestational diabetes mellitus (GDM). This study included 362 neonates of women with GDM and 302 control neonates. The level, lactonase activity, normalized lactonase activity (NLA), and genetic polymorphisms of PON1, serum total oxidant status (TOS), total antioxidant capacity (TAC), and malondialdehyde (MDA) were analyzed. The neonates of the women with GDM had significantly higher levels, lactonase activity, and NLA of PON1, higher TOS, TAC, and MDA concentrations, and relatively higher oxidative stress index than those of the control neonates. The PON1 -108C → T variation decreased the lactonase activity, level, and NLA of PON1, while the PON1 192Q → R variation decreased the PON1 NLA in a genotype-dependent manner in the two groups. Multivariable regression analysis revealed the PON1 -108C/T or 192Q/R variation, apolipoprotein (apo)A1, or apoB as significant predictors of the level, lactonase activity, and NLA of PON1. The lactonase activity, level, and NLA of PON1 were increased in the neonates of women with GDM. The PON1 genetic variants, abnormalities in lipoproteins, and increased oxidative stress may be associated with these changes. This is the first study to report the elevated level, lactonase activity, and NLA of PON1 in the neonates of women with GDM. These neonates also exhibited increased oxidative stress and an adverse glycolipid metabolic profile. We further established that the -108C/T and/or 192Q/R genetic variants of the PON1 gene, abnormalities in lipoprotein metabolism, and/or increased oxidative stress had noticeable influences on the level and activities of PON1. Whether these changes potentially cause metabolic disorders later in life remains to be determined. Therefore, the neonates born to women with GDM require further clinical follow-ups.

Epicardial fat thickness is associated with oxidative stress index in cardiac syndrome X

Aim: We aimed to investigate the relationship between oxidative parameters- total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI)- and EFT in patients with CSX. Without clinically significant coronary artery disease, cardiac syndrome X (CSX) is characterized by cardiac ischemia, and chest pain. However, there is some evidence about increased oxidative stress in CSX patients; the relationship between oxidative stress parameters and epicardial fat thickness (EFT) is not well established in this population. Material and Methods: One hundred and twenty-two patients with normal coronary arteries (73 female, 49 males, mean age 56.71 ± 10.69 years) were enrolled in the study. The study population was divided into two groups according to their OSI. Oxidative stress index below the median was defined as the OSI low group and equal or higher than the median was defined as the OSI high group.Results: EFT was higher in the high OSI group than the low OSI group (6 (3.9 – 8.9) vs. 5.4 (0.56 – 7.2), p= 0.032). In bivariate correlation analysis EFT was positively correlated with OSI and TOS (r=0.242, p=0.036 and r=0.234, p=0.025 respectively). In binary logistic regression analysis, EFT was found to be an independent predictor of OSI.Conclusion: In our study, EFT was independently associated with OSI in CSX patients. This finding suggests that EFT may be a marker of increased oxidative stress in CSX.

Meta-analysis of some physiologic, metabolic and oxidative responses of sheep exposed to environmental heat stress

A meta-analysis was performed from 66 references retained from a total of 147 ones. The aim was to study the relationship between the THI and some physiologic, metabolic and oxidative responses in sheep. The effects of the breed, physiological state and heat relieving strategies were investigated as well. PCA, GLM, one nested ANOVA and global correlations were used to assess the fixed effects.Respiration rate, rectal temperature and heart pulse vary significantly in response to THI increase. However, the magnitude of this variance is defined by the breed and the physiological state of the animal. Moreover, Mechanical heat relieving strategies allowed alleviating the drastic effects of hyperthermia on sheep physiologic responses, whereas some nutritional heat abatement strategies increased metabolic heat load and respiration rate. Antioxidant and mineral supplementations were able to protect animals from heat stress.Environmental-induced hyperthermia reduced feed intake, body weight, circulating glucose and cholesterol levels, and increased lipid and protein catabolism. Significant increase of cortisol hormone was recorded. Indeed, ambient- induced hyperthermia resulted in oxidative stress marked by a decrease of Se contents, enhanced SOD activity, high oxidative stress index, increased protein oxidation, reactive oxygen species production and MDA levels. We report here for the first time that respiration is a second source of free radicals generation in sheep under environmental-induced heat stress.Heat stress disturbs rectal temperature, respiration and heart rates, exacerbates the post absorptive metabolism as well as the antioxidant status of sheep. Therefore, animals increase their maintenance requirements at the expense of production needs, which become not priorities at all.

Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism.

In lung cancer (LC), alterations in redox balance are extensively observed and are a consequence of disease as well as co-occurrent with smoking. We previously demonstrated that metabolic disturbances such as trace element status and carbohydrate metabolism alterations are linked with redox status. The aim of this study was to evaluate relationships between the serum parameters of lipid metabolism and redox balance in LC patients. Serum parameters of lipid metabolism, i.e. total cholesterol (T-C), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglycerides (TG), T-C:HDL-C ratio, non-HDL-C, apolipoprotein A1 (Apo-A1), apolipoprotein B (Apo-B) and Apo-B:Apo-A1 ratio, as well as systemic redox status, i.e. total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), vitamin E (VE), vitamin C (VC), malonyldialdehyde (MDA), conjugated dienes (CD), and 4-hydroxynonenal (4-HNE) were determined in 92 LC patients and 82 control subjects (CS). LC women had significantly lower T-C and LDL-C, and higher TG, while HDL-C, Apo-A1 and Apo-B were significantly decreased in LC patients regardless of sex, when compared to CS. LC men had alterations in the systemic total redox balance such as lower TAS and higher OSI than CS men. LC women had lower VC, but VE was decreased in LC patients, regardless of sex. We observed higher lipid peroxidation in LC patients expressed via higher 4-HNE and CD. Systemic redox disturbances were associated with serum lipid alterations: TOS and OSI were positively correlated with T-C:HDL-C ratio and Apo-B:Apo-A1 ratio and negatively with HDL-C. The parameters of lipid peroxidation CD and MDA were significantly associated with variables reflecting lipid disturbances. The observed correlations were strengthened by general overweight/obesity, abdominal obesity, hypertriglyceridemia and non-smoking status. In conclusion, parameters related to lipid alterations are associated with oxidative stress in LC patients. The largest contribution from lipid parameters was revealed for T-C:HDL-C ratio, HDL-C and Apo-B:Apo-A1 ratio, while the largest contribution from redox status was revealed for OSI and VE. Overweight, obesity, hypertriglyceridemia and non-smoking status intensified these relationships.

Efficacy of drumstick (Moringa oleifera) Leaf-water extract on the modulation of oxidative stress through expression of superoxide dismutase and catalase genes in high fat-fed rats

Obesity-related kidney diseases require an effective approach to avert high fat-diet induced renal damage through oxidative stress. The aim of present study was to evaluate antioxidative strength and protective effects of drumstick (Moringa oleifera) leaf-water extract (DLWE) on kidney in Wistar rats. In this study rats were either fed with control diet or control diet with DLWE or high-fat diet or high-fat diet with DLWE for 16 weeks. The body and kidney weight, oxidative status (lipid peroxidation, ferric reducing antioxidant power, non-enzymatic and enzymatic antioxidants) and gene expression of superoxide dismutase and catalase were assessed. High fat-diet fed rats showed changes in body and kidney weights, increase in lipid peroxidation and decrease in ferric reducing antioxidant power, vitamin C, glutathione, superoxide dismutase and catalase indicating a higher oxidative stress index. At molecular level, high fat-diet caused a significant reduction in gene expression of superoxide dismutase and catalase which was overcome by DLWE supplementation. The oral supplementation of DLWE restored oxidative stress in high fat-diet fed rats. The diet restored lipid peroxidation and kidney antioxidants to control group level. It seems that DLWE has therapeutic potential, so be developed as a supplement to prevent obesity and related oxidative stress.

Antioxidant and Antimicrobial Activities of Salvia multicaulis

The present study aimed to determine antioxidant and antimicrobial activities of Salvia multicaulis Vahl plant collected in Gaziantep province, Turkey. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay kits. Antimicrobial activity was determined with modified agar dilution method. The findings demonstrated that S. multicaulis had high antioxidant activity. However, it is recommended to avoid excessive consumption of the plant due to high OSI. It was also determined that plant extracts possessed antimicrobial potential. As a result, it was determined that the plant can be used as a natural antioxidant and antimicrobial resource.

Serum Paraoxonase, Arylesterase, and Glutathione-S-Transferase Activities and Oxidative Stress Levels in Patients with Mushroom Poisoning

OBJECTIVES:Consumption of toxic species of mushrooms may have detrimental effects and increase oxidative stress. Paraoxonase, arylesterase and glutathione-S-transferase are antioxidants that resist oxidative stress. In this study, we analyzed the changes in these enzymes during intoxication due to mushrooms.METHODS:The study enrolled 49 adult patients with a diagnosis of mushroom poisoning according to clinical findings and 49 healthy volunteers as the control group. The patients with mild clinical findings were hospitalized due to the possibility that the patient had also eaten the mushrooms and due to clinical findings in the late period, which could be fatal. Paraoxonase, arylesterase, and glutathione-S-transferase concentrations, as well as total antioxidant and oxidant status, were determined in the 49 patients and 49 healthy volunteers by taking blood samples in the emergency department.RESULTS:While paraoxonase, arylesterase, and total antioxidant status were significantly decreased in the patient group (p<0.05), glutathione-S-transferase, total oxidant status and the oxidative stress index were significantly higher (p<0.05). There was a positive correlation between the hospitalization time and the oxidative stress index (r=0.752, p<0.001), whereas a negative correlation was found with glutathione-S-transferase (r=-0.420, p=0.003).CONCLUSION:We observed a significant decrease in paraoxonase and arylesterase and an increase in glutathione-S-transferase and oxidative stress indexes in patients with mushroom poisoning, indicating that these patients had an oxidative status. In particular, a low total antioxidant status and high oxidative stress index may gain importance in terms of the assessment of hospitalization duration.

Evaluation of Biomarkers of Oxidative Stress in Attention-Deficit/ Hyperactivity Disorder (ADHD)

Biomarkers of oxidative stress and ADHD in children and adolescents are critically reviewed. They are divided in two groups: biomarkers of oxidative stress (general, proteins, including enzymes and non-protein biomarkers) and biomarkers of specific oxidative damage. Observed associations between concentration levels and ADHD symptoms are nearly always contradictory, which is partly due to aetiological a phenotypic complexity of the disorder. Some trends could be observed: lower ferritin and zinc levels, lower total antioxidant status (TAS), higher total oxidant stress (TOS) and higher oxidative stress index (OSI) are associated with ADHD. Even when there is a correlation most authors claim that this relationship is not causative, as illustrated by placebo-controlled trials reporting conflicting evidence on efficacy of supplementation. Well-defined studies could shed more light on their significance in this disorder by observing changes in concentration levels of the various biomarkers and ADHD symptoms before and after treatment with therapeutics.

Adana ve Antalya’dan Toplanan Ompholatus olearius’un Oksidatif Stres Durumunun Belirlenmesi

The objective of the present study is to determine and compare total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) values and heavy metal content of Omphalotus olearius samples, known as a poisonous mushroom and gathered from Adana (Karaisali) and Antalya (Konyaalti) provinces. TAS, TOS and OSI values were determined using Rel Assay kits. Heavy metal content was measured with atomic absorption spectrophotometer. The results of this study demonstrated that the sample gathered in Antalya had a higher oxidative stress index and generally higher heavy metal content.

Determination of Oxidative Stress Status of Ompholatus olearius Gathered from Adana and Antalya Provinces in Turkey

The objective of the present study is to determine and compare total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) values and heavy metal content of Omphalotus olearius samples, known as a poisonous mushroom and gathered from Adana (Karaisali) and Antalya (Konyaalti) provinces. TAS, TOS and OSI values were determined using Rel Assay kits. Heavy metal content was measured with atomic absorption spectrophotometer. The results of this study demonstrated that the sample gathered in Antalya had a higher oxidative stress index and generally higher heavy metal content.

The GG genotype of vitamin D metabolism-related gene DHCR7 rs12785878 is more prevalent in the Indians and associated with higher oxidative stress index in Singapore

The GG genotype of vitamin D metabolism-related gene DHCR7 rs12785878 is more prevalent in the Indians and associated with higher oxidative stress index in Singapore

Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats.

Chronic arsenic exposure via contaminated drinking water is a global environmental health problem associated with hematological, hepatic and many serious systemic disorders. This study on adult male rats evaluated the protective effects of vitamin E (VE) and vitamin C (VC) against arsenic-mediated hematological and hepatic toxicities. Arsenic was administered orally as arsenic trioxide (3 mg/kg body weight/day), as a single dose for 30 consecutive days or along with VC/ascorbic acid (200 mg/kg body weight/day dissolved in water) and VE/α-tocopherol (400 mg/kg body weight/day dissolved in olive oil) as supplements. Multiple hematological and hepatic parameters were assessed. Arsenic exposure caused significant reduction of erythrocyte counts (p<0.05), leukocyte counts (p<0.01) and hemoglobin (Hb) levels (p<0.01). Arsenic exposure also led to marked echinocytic transformation of erythrocytes resulting in increased morphological index (p<0.001). Altered serum oxidative balance was observed with a higher oxidative stress index (p<0.001). The results also showed a significant increase of serum cholesterol (p<0.05), low-density lipoprotein (p<0.001) and triglycerides (p<0.01), and decreased high-density lipoprotein (p<0.01) along with total protein (p<0.01). A marked elevation of hepatic thiobarbituric acid reactive substance (p<0.05) along with decreased reduced glutathione (p<0.001) levels were also observed. Interestingly, co-administration of VC and VE significantly prevented all the arsenic-induced alterations (p<0.05) except Hb content and serum protein. The present investigation offers strong evidence regarding the protective efficacy of co-administration of VC and VE against hematotoxicity and hepatotoxicity in adult male rats caused by chronic arsenic exposure.

A potential biomarker for fatigue: Oxidative stress and anti-oxidative activity

We sought to determine whether oxidative stress and anti-oxidative activity could act as biomarkers that discriminate patients with chronic fatigue syndrome (CFS) from healthy volunteers at acute and sub-acute fatigue and resting conditions. We calculated the oxidative stress index (OSI) from reactive oxygen metabolites-derived compounds (d-ROMs) and the biological antioxidant potential (BAP). We determined changes in d-ROMs, BAP, and OSI in acute and sub-acute fatigue in two healthy groups, and compared their values at rest between patients with CFS (diagnosed by Fukuda 1994 criteria) and another group of healthy controls. Following acute fatigue in healthy controls, d-ROMs and OSI increased, and BAP decreased. Although d-ROMs and OSI were significantly higher after sub-acute fatigue, BAP did not decrease. Resting condition yielded higher d-ROMs, higher OSI, and lower BAP in patients with CFS than in healthy volunteers, but lower d-ROMs and OSI when compared with sub-acute controls. BAP values did not significantly differ between patients with CFS and controls in the sub-acute condition. However, values were significantly higher than in the resting condition for controls. Thus, measured of oxidative stress (d-ROMS) and anti-oxidative activity (BAP) might be useful for discriminating acute, sub-acute, and resting fatigue in healthy people from patients with CFS, or for evaluating fatigue levels in healthy people.

Relationship between oxidative stress parameters and asymptomatic organ damage in hypertensive patients without diabetes mellitus

Objective. We aimed to evaluate the levels of oxidative stress (OS) parameters such as total antioxidant status or TAS, total oxidant status (TOS), OS index (OSI), paraoxonase 1 (PON1), arylesterase, and total thiol in hypertensive patients with and without asymptomatic organ damage (AOD), and to determine the relationship between these parameters and AOD. Design. Sixty-six patients (21 men, 45 women) with AOD and 66 patients without AOD (21 men, 45 women) were enrolled in the study. Serum OS parameters were measured by colorimetric method. Results. The OSI levels were found to be higher while PON1, PON1/high-density lipoprotein, and arylesterase levels were found to be lower in patients with AOD compared with those in the patients without AOD. Stepwise regression analysis showed high 24-h mean systolic blood pressure, OSI, and low arylesterase level to be independent predictors of AOD. Conclusion. OS level was found to be higher in hypertensive patients with AOD compared with the patients without AOD. However, it is not clear whether increased OS leads to AOD or AOD increases the level of OS. For this purpose, OS level needs to be decreased by antioxidant therapies and patients need to be followed up for a longer duration.