The Gouy phase is related to the axial phase shift experienced by any focused light beam with respect to a reference plane wave when passing through its focus. Generally, the Gouy phase ΦG for a higher-order Hermite-Gaussian (HG) beam is this for the fundamental Gaussian beam multiplied by a factor, which is the sum of the mode indices of the HG beam plus unity. Although this result is a paradigm in optics, we are not aware of its experimental verification. In this paper, we present experimental results obtained with a single-lens interferometer for higher-order HG modes generated using spatial light modulator. The retrieved Gouy phase ΦG is found in a very good quantitative agreement with the mentioned theoretical result.