High Molecular Resin Research Articles

Determination of Taxol, Cephalomannine and 7‐epi‐Taxol in Taxus by PRP‐6 Solid Phase Extraction and High Performance Liquid Chromatography

A simple, sensitive, and precise method for the determination of Taxol,® cephalomannine, and 7‐epi‐taxol in Taxus by solid phase extraction followed by high performance liquid chromatography (HPLC) was developed. In this paper, we describe the use of a novel high molecular resin (PRP‐6) as the loading material in the solid phase extraction column to pretreat the digestion solutions of Taxus. The variables affecting the separation and determination were investigated in detail. Under the selected optimal conditions, Taxol, cephalomannine, and 7‐epi‐taxol in Taxus were determined; the recoveries were in the range of 92.6%–110.9%, RSD were in the range of 4.9%–6.3%, the detection limits were 0.1 µg mL−1. These results can be considered satisfactory.

スメクタイトを含む軟岩の劣化防止に関する研究

Soft rocks, especially smectite bearing tuffs and mud stones, sometimes cause the serious troubles such as ground pressure, landslide and crumbling of cut slope.To study preventive methods of the surface deterioration for smectite bearing soft rocks, experiments in laboratory were carried out. In these experiments, four kinds of preventive methods such as a soak method, a wet curing by covering with mat, an ion exchange method and a seal method have been attemped to six samples of soft rocks. In result, it has become clear to be closely related with exchangeable cation composition of smectite and effect of preventive method. All of the methods are effective to Ca-type smectite bearing soft rocks. On the other hand, only the seal method is applicable to Na-type one.Field test by the seal method was carried out in tunnel consisted of Na-type smectite bearing tuff. The preventive affect using polymer cement or high molecular resin has been ascertained to extend from three to six months. This result indicates that the prevention of deterioration by the seal method is applicable in the case of short term to foundation rock at dam and cut slope etc.

Gel Chromatographic Isolation of Resins and Asphaltene Material in Crude Oils

A new method is described for fast, simple, and quantitative isolation of high molecular resinous and asphaltene material in crude oils without any alteration of the petroleum constituents. The method is based on gel permeation on Sephadex LH-20 using chloroform as the mobile phase which isolates the high molecular weight resins and asphaltene material from the remainder of the crude oil, and a subsequent gel filtration on Sephadex LH-60 separates resins from asphaltenes if required.