Abstract In this study, we investigated landscape variability of the carbon and nitrogen isotopic composition of nonvolant small mammals in the 3 main Brazilian biomes (Amazon, Atlantic Forest, and Cerrado) while also considering the differential spatial distribution of C4 plants in these biomes. We compiled a subset of data on stable carbon and nitrogen isotope ratios from nonvolant small mammals of the order Rodentia and compared the aggregated isotopic variability at the biome level using classical δ13C–δ15N biplot. The concept of isotopic niche width was used to test whether different foraging attributes drive their isotopic composition, while a Bayesian isotopic mixing model was used to estimate the proportion of 3 main food categories available to these small mammals. We also developed isoscapes in the Brazilian biomes, interpolating animals δ13C and δ15N based on the geographic coordinates of each sample and “sourcescapes” considering geographic variation of the dietary sources. The findings of this study advance our understanding of the foraging ecology of small mammals in biodiversity-rich regions of the Neotropics. Classical methodologies such as stomach content confirmed dietary choices revealed by the stable isotopic composition and also highlighted the importance of C3 and C4 plants in the diet of this group of animals, especially in biomes such as the Atlantic Forest and Cerrado, where there is a predominance of highly altered landscapes. These results confirm that replacing the original vegetation with C4 crops has altered the feeding patterns of small mammals, which could lead to critical ecological changes in the trophic structure of these areas. Vertical stratification of the dietary niche and the interaction between biome and foraging lifestyle were also observed. However, in each biome, there was significant intraspecific and interspecific variation caused by preferences for ingestion of plant and animal material, leading to different degrees of omnivory. Finally, the high local variability between individuals and species requires a larger sampling design that may also contribute to improved spatial resolution of the isoscapes.
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