High Linoleic Acid Research Articles

Characterization of Some Olive Varieties Consumed without Pre-Treatment: Naturally Debittered Olives in Türkiye

In table olive production it is necessary to remove oleuropein by brine or dry salting method because it gives bitterness. However, some olive varieties such as Throuba Thassos-Greece, Djemali-Tunisia can be consumed without any de-bittering process. Some olives in Türkiye are also consumed without any pre-treatment and these are Kilis Yağlık, Butko, Hurma (Erkence cv.), and Nizip Yağlık olives. These naturally de-bittered olives were aimed to be characterized in this research. The lowest moisture content was determined for Kilis Yağlık (6.84%) and the highest moisture content was determined for Butko (50.01%). The oil and protein content of the samples was between, 16.66-68.46% and 0.19-18.13%, respectively. Total phenolic content (mg GAE/100g) of Kilis Yağlık, Butko, Hurma and Nizip Yağlık olive varieties were determined as 458.87, 152.09, 109.73, 234.33, respectively. The lowest antioxidant capacity was determined for Butko and the highest value was determined for Kilis Yağlık. The hardness values of the olives were found between 677.44-3688.06 (g). The L*, a*, b* values of olive samples were found between 26.14-32.05, 2.02-4.78, 2.37- 7.18, respectively. Highest oleic was determined for Hurma, highest linoleic acid was determined for Butko whereas the highest linolenic acid was determined for Nizip Yağlık. Volatile component analysis results of olives showed that 24, 23, 16 and 17 volatile components were detected in Kilis Yağlık, Butko, Hurma and Nizip Yağlık olives, respectively.

Optimization of callus induction protocol from leaf explants of P<i>ortulaca oleracea</i> and assessment of fatty acid profiles

A protocol for optimization of callus culture establishment from leaf explants of Portulaca oleracea L. was developed. The effect of ethyl methanesulphonate (EMS) on callus induction and synthesis of fatty acids was evaluated. Callus culture was initiated from leaf explants on Murashige and Skoog’s medium supplemented with 2,4‑dichlorophenoxy acetic acid (2,4‑D), a combination of 2,4‑D + 6‑benzyl adenine (BA) or 2,4‑D + kinetin. The maximum callus biomass was obtained at 2.5 µM BA + 2.5 µM 2,4‑D (20.22 g leaf explant–1 fresh weight). The leaves treated with EMS (0.1 to 0.4% h–1) differentiated callus on this optimized medium combination. The gas chromatography-mass spectrometry analysis of fatty acids indicated that the leaves had a high linolenic acid content (17.72%). The callus cultures synthesized heptadecanoic, oleic, and tricosanoic acids, which were otherwise absent in the leaves. Eicosanoic and docosanoic acids in callus cultures were 2.78 and 3.18 times higher than their content in the leaves, respectively. The diversity of fatty acids in treated callus cultures decreased with increased EMS concentration, but the content of a few individual fatty acids was enhanced. Callus at the dose of 0.1% EMS synthesized linoleic acid, which was two times higher than in the untreated callus, while at 0.2% EMS stearic acid was synthesized, which was absent in the untreated callus. It was concluded that the callus of P. oleracea accumulated different fatty acids, and EMS treatment enhanced the content of a few fatty acids in the cultures.

Unraveling the genetic basis of oil quality in olives: a comparative transcriptome analysis.

The balanced fatty acid profile of olive oil not only enhances its stability but also contributes to its positive effects on health, making it a valuable dietary choice. Olive oil's high content of unsaturated fatty acids and low content of saturated fatty acids contribute to its beneficial effects on cardiovascular diseases and cancer. The quantities of these fatty acids in olive oil may fluctuate due to various factors, with genotype being a crucial determinant of the oil's quality. This study investigated the genetic basis of oil quality by comparing the transcriptome of two Iranian cultivars with contrasting oil profiles: Mari, known for its high oleic acid content, and Shengeh, characterized by high linoleic acid at Jaén index four. Gas chromatography confirmed a significant difference in fatty acid composition between the two cultivars. Mari exhibited significantly higher oleic acid content (78.48%) compared to Shengeh (48.05%), while linoleic acid content was significantly lower in Mari (4.76%) than in Shengeh (26.69%). Using RNA sequencing at Jaén index four, we analyzed genes involved in fatty acid biosynthesis. Differential expression analysis identified 2775 genes showing statistically significant differences between the cultivars. Investigating these genes across nine fundamental pathways involved in oil quality led to the identification of 25 effective genes. Further analysis revealed 78 transcription factors and 95 transcription binding sites involved in oil quality, with BPC6 and RGA emerging as unique factors. This research provides a comprehensive understanding of the genetic and molecular mechanisms underlying oil quality in olive cultivars. The findings have practical implications for olive breeders and producers, potentially streamlining cultivar selection processes and contributing to the production of high-quality olive oil.

Oil polyunsaturated fatty acid composition but not tocopherol levels determine oxylipin formation in plant and algae oils during pan-frying

The oxidation of linoleic acid (LA) and α-linolenic acid (ALA) in plant oils results in primary oxidation products known as oxylipins. It is not known whether the mechanisms of oxidation are driven primarily by oil polyunsaturated fatty acid (PUFA) composition (i.e., more double bonds resulting in more rapid oxidation) or antioxidant (tocopherol) content. The present study addressed this unknown by measuring the effects of pan-frying on LA- and ALA-derived oxylipin concentrations in plant and algae oils with different baseline fatty acid and tocopherol concentrations. Oxylipins, free fatty acids and tocopherols were quantified in algae, olive, corn, canola, and soybean oils before and after 30 minutes of pan-frying. Fatty acid epoxides and hydroxides of LA or ALA were significantly elevated after pan-frying, whereas fatty acid diols were reduced. The level of change in oxylipins was lower in low-PUFA algae and extra virgin olive oils compared to soybean, canola and corn oils (high in PUFAs). Tocopherols decreased significantly after 30 minutes of pan-frying, particularly in oils with low PUFA composition. Minor changes were observed in free fatty acids. Our findings demonstrate that oil PUFA composition is a greater determinant of lipid oxidation than tocopherol content, and that PUFAs in high LA or ALA oils may spare tocopherol oxidation by acting as free radical scavengers.

Effect of a High Linoleic Acid Diet on Pregnant Women and Their Offspring.

Nutritional intake during pregnancy can affect gestational length, fetal development, and impact postnatal growth and health in offspring. Perturbations in maternal nutrition with either an excess or deficiency in nutrients during pregnancy may have harmful effects on the offspring's development and increase the risk of developing chronic diseases later in life. In pregnancy, nutrients transfer from the mother to the fetus via the placenta. Essential fatty acids, linoleic acid (LA) and alpha linoleic acid (ALA), can only be obtained in the diet. In Western countries, the ratio of LA and ALA in the diet has increased dramatically in recent decades. Some animal and human studies have found a correlation between maternal intake of LA and birth weight; however, the association varies. In contrast, some human studies have demonstrated inconclusive findings regarding the correlation between cord blood levels of LA and birth outcomes. In addition, high dietary LA intake in animal studies in pregnancy increased the production of inflammatory markers such as prostaglandins, leukotrienes, cytokines, and tumour necrosis factor-alpha. This review aims to highlight the effect of high dietary LA intake during pregnancy on birth outcomes, obesity, maternal inflammatory markers, and the transfer of fatty acids across the placenta.

Identification of High Linoleic Acid Varieties in Tetraploid perilla through Gamma-ray Irradiation and CRISPR/Cas9.

Perilla [Perilla frutescens (L.) var frutescens] is a traditional oil crop in Asia, recognized for its seeds abundant in α-linolenic acid (18:3), a key omega-3 fatty acid known for its health benefits. Despite the known nutritional value, the reason behind the higher 18:3 content in tetraploid perilla seeds remained unexplored. Gamma irradiation yielded mutants with altered seed fatty acid composition. Among the mutants, DY-46-5 showed a 27% increase in 18:2 due to the 4-bp deletion of PfrFAD3b, and NC-65-12 displayed a 16% increase in 18:2 due to the loss of function of PfrFAD3a through a large deletion. Knocking out both copies of FATTY ACID DESATURASE3 (PfrFAD3a and PfrFAD3b) simultaneously using CRISPR/Cas9 resulted in an increase in 18:2 by up to 75% and a decrease in 18:3 to as low as 0.3% in seeds, emphasizing the pivotal roles of both genes in 18:3 synthesis in tetraploid perilla. Furthermore, diploid Perilla citriodora, the progenitor of cultivated tetraploid perilla, harbors only PfrFAD3b, with a fatty acid analysis revealing lower 18:3 levels than tetraploid perilla. In conclusion, the enhanced 18:3 content in cultivated tetraploid perilla seeds can be attributed to the acquisition of two FAD3 copies through hybridization with wild-type diploid perilla.

Plasma n6 polyunsaturated fatty acid levels and risk for total and cause-specific mortality: A prospective observational study from the UK Biobank

BackgroundThe potential role of n-6 PUFAs in major health outcomes remains controversial. ObjectivesTo examine the relationship between the major plasma n6 PUFA, linoleic acid (LA), as well as the non-LA n6 PUFAs, and total and cause-specific mortality. MethodsThis was a prospective, observational, biomarker-based study in the UK Biobank. Individuals with complete information on baseline demographic, covariate and plasma PUFA levels (percent ot total fatty acids) and mortality outcomes were included (n=257,925). Multivariable-adjusted, Cox-proportional hazards models were used to predict risk of death from all-causes, and from cardiovascular disease (CVD), cancer, and other causes as a function of plasma LA and non-LA n6 levels, both continuously and by PUFA quintile (Q). ResultsComparing LA Q5 to Q1, the hazard ratio (HR, 95% CI) for total mortality was 0.80 (0.76, 0.84; p<0.001), and this was similar for all three cause-specific death categories. On the other hand, mortality HR for non-LA n6 was 1.12 (1.08,1.17; p<0.001), and this was primarily due to increased risk for non-CVD, noncancer deaths [HR 1.29 (1.19,1.40; p<0.001)]. Exploratory analyses among the eight next most common other causes of death suggested that both the decreased risk associated with higher LA and the increased risk associated with non-LA n6 were confined to deaths from respiratory and digestive diseases. ConclusionsThese findings highlight the profound differences in mortality risk related to LA and non-LA n6 PUFA levels and underscore the inappropriateness of treating n-6 PUFAs as a homogenous class with respect to health outcomes. They also support recommendations to maintain (if not increase) current LA intakes.

Camelina sativa Seeds and Oil as Ingredients in Model Muffins in Order to Enhance Their Health-Promoting Value.

The aim of this study was to see whether it is possible to add camelina oil and seeds as ingredients in muffins in order to enhance their health-promoting value, such as their bioactive compound content, while maintaining the organoleptic attributes considered desirable by consumers. Camelina oil is characterised by a high linolenic acid content. Four types of muffins were prepared for analysis: MBnO-control muffins (containing 11.85% rapeseed oil), MCsO-muffins containing camelina oil instead of rapeseed oil, MCsS-muffins containing 6.65% camelina seeds in relation to the mass of prepared dough, and MCsOS-muffins containing both camelina oil and camelina seeds. The change in the fatty acid profile in muffins with the addition of camelina oil was significant; however, it was found that, as a result of thermal treatment, lower amounts of saturated fatty acids were formed. Among all the investigated experimental variants, muffins were characterised by the highest contents of all the phenolic acids analysed. The substitution of rapeseed oil with camelina oil had no negative effect on most of the organoleptic attributes of the muffins. Moreover, thanks to a greater content of carotenoids, camelina oil had an advantageous effect on the improvement of product colour, thus improving its overall desirability.

The Lipid–Heart Hypothesis and the Keys Equation Defined the Dietary Guidelines but Ignored the Impact of Trans-Fat and High Linoleic Acid Consumption

In response to a perceived epidemic of coronary heart disease, Ancel Keys introduced the lipid–heart hypothesis in 1953 which asserted that high intakes of total fat, saturated fat, and cholesterol lead to atherosclerosis and that consuming less fat and cholesterol, and replacing saturated fat with polyunsaturated fat, would reduce serum cholesterol and consequently the risk of heart disease. Keys proposed an equation that would predict the concentration of serum cholesterol (ΔChol.) from the consumption of saturated fat (ΔS), polyunsaturated fat (ΔP), and cholesterol (ΔZ): ΔChol. = 1.2(2ΔS − ΔP) + 1.5ΔZ. However, the Keys equation conflated natural saturated fat and industrial trans-fat into a single parameter and considered only linoleic acid as the polyunsaturated fat. This ignored the widespread consumption of trans-fat and its effects on serum cholesterol and promoted an imbalance of omega-6 to omega-3 fatty acids in the diet. Numerous observational, epidemiological, interventional, and autopsy studies have failed to validate the Keys equation and the lipid–heart hypothesis. Nevertheless, these have been the cornerstone of national and international dietary guidelines which have focused disproportionately on heart disease and much less so on cancer and metabolic disorders, which have steadily increased since the adoption of this hypothesis.

Usability of Hemp Plant in Poultry Nutrition

Hemp (Cannabis sativa L.) is an annual plant with 2n=20 chromosomes, one-year, C3 group, cultivated for its long and strong fibers and seeds. It is a plant that should be cultivated in a controlled manner due to the presence of THC in its natural structure. Producers who avoid control and monitoring have turned to alternative products instead of hemp farming. Countries that cultivate cannabis in large areas; France, China, and Canada. Some countries are updating their cannabis-related laws and making efforts to increase hemp acreage. It is known that hemp farming has been practiced in Anatolia since 1500 BC. Various products such as hemp seeds, hemp pulp, hemp oil have been used in various studies in poultry feeding studies, while no negative results have been encountered, but various positive results have been suggested and their use is recommended. It is of great importance to investigate the effects of cannabidiols, which have strong antimicrobial, antioxidant and immunostimulant effects, as they are expected to support the immune system in poultry, especially broilers. Some of the studies in which hemp products are used in poultry nutrition are as follows; Addition of hemp meal to laying hen diets has been reported to make no significant difference in egg production, feed consumption, feed utilization, body weight gain or egg quality, but results in lower palmitic acid concentrations and higher Linoleic acid (LA) and alpha-linolenic acid (ALA) concentrations, which are healthier for human consumption. Hemp seeds and hemp oil used in chicken rations caused an increase in the omega-3 polyunsaturated fatty acid content and color intensity of egg yolks, but no adverse effects were observed on the sensory profiles of cooked eggs. Addition of cannabis meal to fast growing broiler diets did not affect performance or mortality, and no effect on the number of Clostridium perfringens in the cecum was reported. Hemp seeds have a remarkable effect on the growth of broiler chicks and can help reduce feed expenditures for broiler rearing. In this paper, the use and usability of the hemp plant, whose production areas are expanding rapidly, in poultry will be examined.

Hyperspectral imaging predicts free fatty acid levels, peroxide values, and linoleic acid and oleic acid concentrations in tree nut kernels

Imaging technologies are advancing rapidly in food processing systems to reduce food waste. However, modelling techniques, sample sizes and dataset proportioning methods significantly affect the performance of models in predicting food quality variables. This study examined the potential of hyperspectral imaging to predict peroxide values (PV), free fatty acid (FFA) levels and fatty acid concentrations, including oleic and linoleic acid, in two tree nuts, canarium and macadamia. The effectiveness of artificial neural network (ANN) regression and partial least squares regression (PLSR) techniques to make these predictions was examined. Additionally, the importance of the dataset size on prediction accuracy and the dataset-proportioning method for developing predictive models were assessed. Both ANN and PLSR models predicted FFA levels and oleic acid concentrations with high accuracy, but PV and linoleic acid concentrations were predicted poorly. Changing the test-dataset proportioning method for the small dataset led to comparable R2test values by both ANN and PLSR in predicting FFA levels. Successful prediction of FFA levels could be explained partly by high variability, even in the dataset with the small number of samples. The study highlights the significance of factors like dataset size, test-dataset proportioning method, and model selection when predicting the quality attributes.

Application of rheology and interfacial rheology to investigate the emulsion stability of ultrasound-assisted cross-linked myofibrillar protein: Effects of oil phase types

This work was designed to illustrate the differences in the dispersed phase that affect the emulsion stability. The effect of oil types including soy oil, corn oil, rapeseed oil, sunflower oil, and flaxseed oil on the stability of the emulsion stabilized by modified MP (sonication after crosslinking treatment) was investigated. Results showed that the differences in fatty acid composition in the oil phase would affect the interaction between the oil phase and modified MP. Emulsion in the P-flaxseed group (with higher linolenic acid) showed lower TSI values, smaller protein-coated oil droplets, higher adsorbed protein content, storage modulus, and small elastic Lissajous curve area than other emulsion groups. While the P-rapeseed (with higher oleic acid) and P-sunflower (with higher linoleic acid) groups had large droplet sizes, poor emulsion stability, and rheological behavior. Correspondingly, the higher θo/w (86.97°), hf, π (21.55 mN/m), Kdiff, KR, Ed, E-π slope (3.22), and linear interfacial Lissajous plot in the P-flaxseed group indicated modified MP was preferred to adsorb on the surface of flaxseed oil to form an elastic-dominated interfacial protein film with higher deformation resistance. Furthermore, the poorer interfacial adsorption behavior and easily collapsed interfacial film further proved the poor emulsion stability of the P-rapeseed and P-sunflower groups. Therefore, differences in fatty acid compositions of oil phases impacted the interfacial adsorption characteristics of the modified MP, thereby influencing the emulsion stability.

RNA Sequencing Reveals the Inhibitory Effect of High Levels of Arachidonic Acid and Linoleic Acid on C2C12 Differentiation and Myogenic Biomarkers.

Over the past three decades, studies have shown that consuming polyunsaturated fatty acids (PUFAs) can enhance animal and human health and welfare through biological, biochemical, pathological, and pharmacological impacts. Furthermore, omega-6 plays key roles in the cardiopulmonary system, including promoting airway relaxation and inhibiting atherosclerosis and hypertension. However, findings from investigations of the effects of omega-6 fatty acids on molecular and cellular activity and discussions on their influence on biomarkers are still unclear. Therefore, the present study aimed to evaluate omega-6 fatty acids, the arachidonic acid (AA), and linoleic acid (LA) effects on C2C12 proliferation, myogenesis morphology, and relative myogenic biomarker expression through the Wnt pathway. C2C12 cells were cultured with and without 25, 50, 100, and 150 µM of LA and AA and then subjected to CCK8, Giemsa staining, RT qPCR, Western blotting, and RNA Sequencing. The CCK8 Assay results showed that 25, 50, 100, and 150 µM LA significantly decreased the viability after 72 h for 25, 50, 100, and 150 µM concentrations. Also, AA supplementation decreased cell viability after 24 h for 150 µM, 48 h for 150 µM, and 72 h for 50, 100, and 150 µM concentrations. Moreover, the LA and AA inhibitory effects noticed through Gimesa staining were morphological changes during myoblast differentiation. Both LA and AA showed inhibiting IGF1, Cola1, Col6a2, Col6a1, Itga10, Itga11, SFRP2, DAAM2, and NKD2 effects; however, the depressing effect was higher for AA compared to LA. The previous results were confirmed through Western blotting, which showed that 50 µM LA and AA significantly reduced DAAM2 and SFRP2 protein levels compared to the control. Regarding RNA sequencing results, LA and AA increased the number of differentially expressed (DE) Mt-rRNA and snoRNA; however, the numbers of lncRNA detected decreased compared to the control. Our findings demonstrate that high and moderate LA and AA concentrations reduce primary myoblast proliferation and differentiation. Also, they highlight novel biomarkers and regulatory factors to improve our understanding of how the nutrition of fatty acids can control and modulate the myogenesis and differentiation process through different biomarker families.

Maternal Diet High in Linoleic Acid Alters Offspring Lipids and Hepatic Regulators of Lipid Metabolism in an Adolescent Rat Model.

Linoleic acid (LA), an n-6 polyunsaturated fatty acid (PUFA), is essential for fetal growth and development. A maternal high LA (HLA) diet alters cardiovascular development in adolescent rats and hepatic function in adult rats in a sex-specific manner. We investigated the effects of an HLA diet on adolescent offspring hepatic lipids and hepatic lipid metabolism gene expression, and the ability of the postnatal diet to alter these effects. Female Wistar Kyoto rats were fed low LA (LLA; 1.44% energy from LA) or high LA (HLA; 6.21% energy from LA) diets during pregnancy and gestation/lactation. Offspring, weaned at postnatal day (PN) 25, were fed LLA or HLA and euthanised at PN40 (n = 6-8). Maternal HLA increased circulating uric acid, decreased hepatic cholesterol and increased hepatic Pparg in males, whereas only hepatic Srebf1 and Hmgcr increased in females. Postnatal (post-weaning) HLA decreased liver weight (% body weight) and increased hepatic Hmgcr in males, and decreased hepatic triglycerides in females. Maternal and postnatal HLA had an interaction effect on Lpl, Cpt1a and Pparg in females. These findings suggest that an HLA diet both during and after pregnancy should be avoided to improve offspring disease risk.

High linoleic acid levels in red blood cells predict a poor response to neoadjuvant chemotherapy in human epidermal growth factor receptor type 2-positive breast cancer patients

ObjectivePolyunsaturated fatty acids are categorized as ω-3 or ⍵-6. Previous studies demonstrate that breast cancers display a high expression of fatty acid synthase and high fatty acid levels. Our study sought to determine if changes in plasma or red blood cell membrane fatty acid levels were associated with the response to preoperative (neoadjuvant) chemotherapy in non-metastatic breast cancer patients. MethodsOur prospective study assessed fatty acid levels in plasma and red blood cell membrane. Response to neoadjuvant chemotherapy was evaluated by the presence or absence of pathologic complete response and/or residual cancer burden. ResultsA total of 28 patients were included. First, patients who achieved pathologic complete response had significantly higher neutrophil-to-lymphocyte ratio versus no pathologic complete response (P = 0.003). Second, total red blood cell membrane polyunsaturated fatty acids were higher in the absence of pathologic complete response (P = 0.0028). Third, total red blood cell membrane ⍵-6 polyunsaturated fatty acids were also higher in no pathologic complete response (P < 0.01). Among ⍵-6 polyunsaturated fatty acids, red blood cell membrane linoleic acid was higher in the absence of pathologic complete response (P < 0.01). Notably, plasma polyunsaturated fatty acid, ⍵-6, and linoleic acid levels did not have significant differences. A multivariate analysis confirmed red blood cell membrane linoleic acid was associated with no pathologic complete response; this was further confirmed by receiver operating characteristic analysis (specificity = 92.3%, sensitivity = 76.9%, and area under the curve = 0.855). ConclusionsPending further validation, red blood cell membrane linoleic acid might serve as a predictor biomarker of poorer response to neoadjuvant chemotherapy in non-metastatic human epidermal growth factor receptor type 2–positive breast cancer. Measuring fatty acids in red blood cell membrane could offer a convenient, minimally invasive strategy to identifying patients more likely to respond or those with chemoresistance.

Diversity analysis of linseed germplasm for quantitative traits

132 linseed germplasm evaluated for ten morphological traits and fatty acid profiles indicated ample variability for the studied traits. BRLS 120 recorded high yield and 1000 grain weight along with high linolenic acid content. The genotypes grouped in to seven clusters indicating the diversity among the germplasm.

Identification of genes associated with fatty acid biosynthesis based on 214 safflower core germplasm

BackgroundSafflower (Carthamus tinctorius L.) is an oilseed crop with substantial medicinal and economic value. However, the methods for constructing safflower core germplasm resources are limited, and the molecular mechanisms of lipid biosynthesis in safflower seeds are not well understood.ResultsIn this study, 11 oil-related quantitative traits and 50 pairs of InDel markers were used to assess the diversity of a collection of 605 safflower germplasms. The original safflower germplasm exhibited rich phenotypic diversity, with high variation for most of the phenotypic traits under investigation. Similarly, high genetic diversity was evaluated in the original germplasm, in which the mean Shannon’s information index (I), observed heterozygosity (H0), and expected heterozygosity (He) were 0.553, 0.182, and 0.374, respectively. Four subgroups with strong genetic structures were identified and a core germplasm of 214 cultivars was constructed, which is well represented in the original germplasm. Meanwhile, differential expression analysis of the transcriptomes of high and low linoleic acid safflower varieties at two stages of seed development identified a total of 47 genes associated with lipid biosynthesis. High expression of the genes KAS II and SAD enhanced the synthesis and accumulation of oleic acid, while FAD genes like FAD2 (Chr8G0104100), FAD3, FAD7 and FAD8 promoted the consumption of oleic acid conversion. The coordinated regulation of these multiple genes ensures the high accumulation of oleic acid in safflower seed oil.ConclusionsBased on these findings, a core germplasm of 214 cultivars was constructed and 47 candidate genes related to unsaturated fatty acid biosynthesis and lipid accumulation were identified. These results not only provide guidance for further studies to elucidate the molecular basis of oil lipid accumulation in safflower seeds, but also contribute to safflower cultivar improvements.

Causal relationship between linoleic acid and type 2 diabetes and glycemic traits: a bidirectional Mendelian randomization study.

To investigate the causal relationships between linoleic acid and type 2 diabetes, and between linoleic acid and glycemic traits in European populations. This study employed a two-sample Mendelian randomization approach to infer causality between linoleic acid and type 2 diabetes, as well as between linoleic acid and glycemic traits, leveraging genetic variations. Data were sourced from genome-wide association study summary datasets. Random-effects inverse-variance weighted, weighted median, and MR-Egger methods were used for the two-sample Mendelian randomization analyses. Results were presented as odds ratios with a 95% confidence interval. Multiple sensitivity analyses were conducted to assess result robustness. MR findings indicated a correlation between linoleic acid levels and the risk of type 2 diabetes, fasting blood glucose, and glycated hemoglobin (HbA1c), but not with fasting insulin. Specifically: type 2 diabetes (OR: 0.811, 95% CI: 0.688-0.956, P=0.013<0.05),fasting blood glucose (β_IVW): -0.056, 95% CI: (-0.091,-0.021), P=0.002< 0.0125), glycated hemoglobin (β_IVW: -0.032, 95% CI: (-0.048,-0.015), P=0.0002< 0.0125) and Fasting insulin (β_IVW: -0.024, 95% CI: (-0.056,-0.008), P=0.136 >0.05).Reverse MR analyses showed a correlation between type 2 diabetes and reduced levels of linoleic acid (β_IVW: -0.033, 95% CI: (-0.059,-0.006), P=0.014<0.05). Multiple sensitivity analyses also detected study heterogeneity but found no evidence of horizontal pleiotropy. High levels linoleic acid can reduce the risk of type 2 diabetes, fasting blood glucose, and glycated hemoglobin, but has no significant relation with fasting insulin. Type 2 diabetes can lower linoleic acid levels; however, no significant causal relationship was observed between the three glycemic traits and reduced levels of linoleic acid.

Morphological, Biochemical, and Molecular Characterization of Exotic Brassica Germplasm.

Oilseed rape (Brassica napus L.) is an important oilseed crop. We examined the diversity of germplasm expressed at three distinct levels (i.e., morphological, biochemical, and DNA levels). In this study, 150 B. napus L. accessions with three check varieties were provided by Bioresources Conservation Institute. The germplasm was grown in field conditions for data collection of 15 quantitative and nine qualitative agro-morphological traits. The result indicated that for 15 quantitative agro-morphological traits, the highest coefficient of variation was recorded for plant height and days to flowering initiation. For nine qualitative traits, most of the accessions have a spatulate leaf, brown color seeds, yellow flowers, and erect silique attitude. The best adoptable genetically diverse exotic Brassica germplasms were selected, i.e., accessions 24178, 24881, 24199, 24214, 24242, and 24192. Based on biochemical analysis for high oil content and high oleic acid content, chakwal sarsoon and accession 24192 were selected. For high oleic and linoleic acids, accession 24181 performed best, for low erucic acid accessions 24177 and 24195. Based on molecular (SSR) markers, the top 50 selected genotypes were evaluated with 30 SSR markers. The 47 genotypes with three check varieties were clustered in six major groups; the coefficient of similarity ranged between 0.18 and 1.00. Based on SSR data, the germplasms accession 24178 and Abasin were the most diverse genotypes. These genotypes have the capacity and could be used in future breeding programs. High genetic variations were investigated through the SSR among the studied genotypes of Brassica napus L. The present study also concluded that SSR is a better technique for intraspecific genetic diversity. Other modern techniques should be applied such as SNIP for the investigation of a high level of genetic diversity among crop plants in the future.

Screening deep eutectic solvents as green extractants for oil from plant seeds based on COSMO-RS model

Deep eutectic solvents (DESs) have recently attracted considerable interest as eco-friendly alternatives for conventional solvents in many industrial separation processes. However, due to its vast number of possible combinations, reliable approaches for guiding the selection of task-specific DES remain limited. Using conductor-like screening model for real solvent (COSMO-RS), the solubility capacity was applied to screen 126 deep eutectic solvents to estimate their potential use as plant seed oil extractants. Moreover, DESs’ σ-profiles were matched to σ-profiles of linoleic acid and glutamine, representing oil and oil protective protein membrane, to evaluate their molecule interactions. Results of COSMO-RS revealed that the DESs with higher linoleic acid solubility capacity showed lower solubility capacity for protein and vice versa. Moreover, σ-profiles of representative DESs demonstrated this behavior by comparing the similarity of DESs to both linoleic acid and protein. Consequently, DESs with high linoleic acid solubility could be used as individual solvents, while DESs with high protein solubility could be used as co-solvents with organic solvents. However, the experimental results proved that the use of DESs having high protein capacity, as co-solvents with diethyl ether, n-pentane and n-heptane raised the extracted oil yield. Utilizing ChCl:Gly (1:2) as a co-solvent with diethyl ether produced the highest yield of 30.7% compared to pure diethyl ether, which produced 27.0%.