High-level Activity Research Articles

Co/Co3O4@NC-CNTs modified separator of Li-S battery achieving the synergistic effect of adsorption-directional migration-catalysis via built-in electric field

The shuttle effect of lithium polysulfides (LiPSs) and sluggish sulfur conversion kinetics have seriously hindered the commercial application of lithium-sulfur (Li-S) batteries. Currently, the adsorption and catalysis processes are emphasized; however, the diffusion process is often neglected. The delayed diffusion of the adsorbed LiPSs significantly reduce battery performance. Herein, the directional migration of Sn2- was realized by adjusting the characteristics of heterostructure materials. The heterostructure consists of Co with a high Fermi level and excellent catalytic activity and Co3O4 with a low Fermi level and strong adsorption ability. This configuration regulated the direction of the built-in electric field (BIEF) at the heterogeneous interface, which promoted the migration of Sn2− from Co3O4 to Co side and realised a continuous “adsorption-directional migration-catalysis” mechanism. Experimental and theoretical results indicated that the Co/Co3O4 heterostructure modified by nitrogen-doped carbon nanotubes (Co/Co3O4@NC-CNTs), as the separator of Li-S batteries, not only enhanced the adsorption of LiPSs but also accelerated the kinetic conversion process. Consequently, the battery modified by the Co/Co3O4@NC-CNTs separator exhibited a high initial specific capacity of 1423 mAh g−1 at 0.2C, and maintained 735.5 mAh g−1 at a current density of 1C after 400 cycles.

Brain-Inspired Image Perceptual Quality Assessment Based on EEG: A QoE Perspective.

Human-oriented image communication should take the quality of experience (QoE) as an optimization goal, which requires effective image perceptual quality metrics. However, traditional user-based assessment metrics are limited by the deviation caused by human high-level cognitive activities. To tackle this issue, in this paper, we construct a brain response-based image perceptual quality metric and develop a brain-inspired network to assess the image perceptual quality based on it. Our method aims to establish the relationship between image quality changes and underlying brain responses in image compression scenarios using the electroencephalography (EEG) approach. We first establish EEG datasets by collecting the corresponding EEG signals when subjects watch distorted images. Then, we design a measurement model to extract EEG features that reflect human perception to establish a new image perceptual quality metric: EEG perceptual score (EPS). To use this metric in practical scenarios, we embed the brain perception process into a prediction model to generate the EPS directly from the input images. Experimental results show that our proposed measurement model and prediction model can achieve better performance. The proposed brain response-based image perceptual quality metric can measure the human brain's perceptual state more accurately, thus performing a better assessment of image perceptual quality.

Association of overweight and obesity with gestational diabetes mellitus among pregnant women attending antenatal care clinics in Addis Ababa, Ethiopia: a case-control study

ObjectiveMaternal obesity and gestational diabetes mellitus (GDM) are becoming major public health concerns in developing countries. Understanding their relationship can help in developing contextually appropriate and targeted prevention strategies and...

Interpretable adversarial example detection via high-level concept activation vector

Deep neural networks have achieved amazing performance in many tasks. However, they are easily fooled by small perturbations added to the input. Such small perturbations to image data are usually imperceptible to humans. The uninterpretable nature of deep learning systems is considered to be one of the reasons why they are vulnerable to adversarial attacks. For enhanced trust and confidence, it is crucial for artificial intelligence systems to ensure transparency, reliability, and human comprehensibility in their decision-making processes as they gain wider acceptance among the general public. In this paper, we propose an approach for defending against adversarial attacks based on conceptually interpretable techniques. Our approach to model interpretation is on high-level concepts rather than low-level pixel features. Our key finding is that adding small perturbations leads to large changes in the model concept vector tests. Based on this, we design a single image concept vector testing method for detecting adversarial examples. Our experiments on the Imagenet dataset show that our method can achieve an average accuracy of over 95%. We provide source code in the supplementary material.

The substantial role of sleep, stress and physical activity in persistent high levels of fatigue in patients with inflammatory bowel disease: A longitudinal trajectory study.

Fatigue is a common and burdensome problem for patients with inflammatory bowel disease (IBD). Previous studies lack insight into the individual variability in fatigue severity and course over time, and the characteristics of patients at risk of severe and persistent fatigue. This study aimed to identify distinct groups of IBD patients based on their level and course of fatigue over five years. Subsequently, we examined the relationship between these trajectories, patient characteristics and trajectories of perceived stress, sleep and physical activity. This longitudinal cohort study used prospectively collected data from the myIBDcoach telemedicine monitoring tool, including 320 IBD patients who completed three or more online consultations between 2016 and 2021. Latent class growth analyses were performed to identify distinct trajectories. We found five subgroups with distinct trajectories of fatigue, differing in level and course over time, with 33% of patients experiencing chronic stable and high levels of fatigue. Few differences in patient characteristics were found between trajectories, yet the chronic high fatigue subgroup was more likely to report persistent stable sleep problems, perceived stress, and little physical activity over time compared to the other groups. Distinct subgroups of IBD patients can be identified based on longitudinal fatigue trajectories. The relatively stable levels of fatigue, stress, sleep, and physical activity suggest that a one-time screening of patients on these topics may be sufficient to identify those at risk. Interventions aiming to reduce fatigue should target persistent stress, sleep problems, and low levels of physical activity.

한반도 주변 해역 표층 수온 그래프를 해석하는 과정에서 중학생들이 인식하는 인지적 부담에 대한 연구

Objectives The purpose of this study is to identify the cognitive load that middle school students perceive in interpreting of sea surface temperature around the Korean Peninsula according to the type of graph, and to help middle school teachers develop teaching and learning materials by providing information related to cognitive load. Methods For This, we collected and analyzed data on the fixation duration and pupil diameter of middle school students(N=32) while interpreting of sea surface temperature around the Korean Peninsula using the eye tracker(Tobii Pro Glass 2 model). And we conducted an independent sample t-test using the Exel on two eye tracking data to confirm the cognitive load between the two groups. Results The results were as follows. First, the fixation duration rate was higher in the data area than in the legend area when middle school students read the sea surface temperature at the contoured graph. However, it was the opposite when they read the sea surface temperature at the colored graph. They depended on the legend area when reading the colored graph rather than when reading the contoured graph. Second, In all the processes of middle school students interpreting the graph, their pupil diameter was expanded compared to the standard pupil diameter. Their pupil diameter expanded more and more as they progressed to Step 1(the activity of reading temperature), Step 2(the activities to compare temperature gradients), and Step 3(the activities to find cause of the same temperature). The middle school students’ pupil diameter was further expanded when interpreting the colored graph rather than when interpreting the contoured graph. Conclusions Middle school students had more cognitive load when interpreting the colored graph rather than when interpreting the contoured graph. And they had more cognitive load in high-level activities than in low-level activities in the process of interpreting graphs. Therefore, it is necessary to reconstruct the graph of sea surface temperature around the Korean Peninsula to relieve the middle school students’ cognitive load.

Optimal lifestyle patterns for delaying ageing and reducing all-cause mortality: insights from the UK Biobank

BackgroundWith the rapid aging of the global population, identifying lifestyle patterns that effectively delay aging and reduce mortality risk is of paramount importance. This study utilizes the UK Biobank to analyze the associations of the Dietary Inflammatory Index, physical activity, and sleep on biological aging and all-cause mortality.MethodsA prospective cohort study was conducted using data from over half a million UK Biobank participants. Two datasets were created by subjective and objective measurements of physical activity: the Subjective Physical Activity (SPA) and Objective Physical Activity (OPA) datasets. Lifestyle patterns, including diet habits, exercise levels, and sleep quality, were assessed within these datasets. Biological aging was quantified using validated methods, including Homeostatic Dysregulation, Klemera-Doubal Method Biological Age, Phenotypic Age, and Telomere Length. All-cause mortality data were obtained from the National Health Service. Statistical analyses included weighted linear regression and Cox proportional hazard models, adjusted for a range of covariates.ResultsThe findings indicate that, in most cases, maintaining an anti-inflammatory diet, engaging in at least moderate physical activity, and ensuring healthy sleep conditions are associated with delayed physiological aging (Cohen’s d ranging from 0.274 to 0.633) and significantly reduced risk of all-cause mortality (HR-SPA: 0.690, 95% CI: 0.538, 0.884; HR-OPA: 0.493, 95% CI: 0.293, 0.828). These effects are particularly pronounced in individuals under 60 years of age and in women. However, it was observed that the level of physical activity recommended by the World Health Organization (600 MET-minutes/week) does not achieve the optimal effect in delaying biological aging. The best effect in decelerating biological aging was seen in the high-level physical activity group (≥ 3000 MET-minutes/week). The study also highlights the potential of biological age acceleration and telomere length as biomarkers for predicting the risk of mortality.ConclusionsChoosing healthy lifestyle patterns, especially an anti-inflammatory diet, at least moderate physical activity, and healthy sleep patterns, is crucial for delaying aging and reducing mortality risk. These findings support the development of targeted interventions to improve public health outcomes. Future research should focus on objective assessments of lifestyle to further validate these associations.

Massively parallel reporter assays identify enhancer elements in oesophageal Adenocarcinoma.

Cancer is a disease underpinned by aberrant gene expression. Enhancers are regulatory elements that play a major role in transcriptional control and changes in active enhancer function are likely critical in the pathogenesis of oesophageal adenocarcinoma (OAC). Here, we utilise STARR-seq to profile the genome-wide enhancer landscape in OAC and identify hundreds of high-confidence enhancer elements. These regions are enriched in enhancer-associated chromatin marks, are actively transcribed and exhibit high levels of associated gene activity in OAC cells. These characteristics are maintained in human patient samples, demonstrating their disease relevance. This relevance is further underlined by their responsiveness to oncogenic ERBB2 inhibition and increased activity compared to the pre-cancerous Barrett's state. Mechanistically, these enhancers are linked to the core OAC transcriptional network and in particular KLF5 binding is associated with high level activity, providing further support for a role of this transcription factor in defining the OAC transcriptome. Our results therefore uncover a set of enhancer elements with physiological significance, that widen our understanding of the molecular alterations in OAC and point to mechanisms through which response to targeted therapy may occur.

Impact of patent infringement damages on firms' innovation performance: the moderating effects of patent-intensive industries

PurposeThe patent thickets exacerbate patent infringement, especially in patent-intensive industries. Damages are a crucial remedy for patent infringement, upholding innovation incentives in the patent system. This paper aims to examine the impact of damages on the subsequent innovation performance of plaintiff and defendant firms and the moderating role of patent-intensive industries in these effects.Design/methodology/approachBased on data from 1,062 Chinese firms involved in patent infringement litigation, this paper uses Poisson panel regression models to examine the dynamic impact of damages on incremental and breakthrough innovation performance for plaintiff and defendant firms and further validates the moderating role of patent-intensive industries on the impacts. Additionally, this paper conducts heterogeneity analysis by categorizing the sample into micro and small enterprises (MSEs) and medium and large enterprises (MLEs).FindingsThis paper shows that MLE plaintiffs skew toward incremental rather than breakthrough innovations after awarding damages, whereas MSEs transiently engage in high-level innovation activities. (2) Plaintiffs with higher payout ratios are more inclined toward undertaking breakthrough innovations. (3) Awarded damages significantly inhibit the innovative output of defendants at varying levels of innovation. (4) Plaintiff firms in patent-intensive industries show more innovation after awarding damages than those in other sectors, but damages do not effectively encourage MSEs to innovate. Defendants in patent-intensive industries are also more reluctant to innovate after damages.Originality/valueThis paper empirically investigates the relationship between patent damages and firms' subsequent innovation performance in terms of litigation status, organizational scale and level of technological innovation. Besides, this paper reveals the differences in the impact of patent protection on firms' innovation in patent-intensive industries versus other industries.

Surface electromyographic analysis of the bilateral abdomen and back muscle during selected yoga posture

Sedentary lifestyles lead to postural deformities, Lower Back Pain(LBP) and Colorectal cancer(CRC). Practicing Yoga attains definite strength in muscles by activating muscle fibers. The Yoga poses analyzed are often used in strengthening and stability programs for the abdomen and back muscles. ObjectiveTo determine the potential use of Dolphin pose and Dolphin plank in specific strengthening endurance and rehabilitation programs via sEMG analysis in the selected bilateral abdomen and lower back muscles. MethodsSurface electromyographic analysis was conducted on bilateral muscles, including the Rectus Abdominis(RA), External oblique(EO), Internal oblique(IO), and Erector Spinae(ES), resulting in Dolphin pose(DS), Dolphin plank(DP), and High plank(HP). Data were expressed by Root Mean Square(RMS) of each bilateral muscle during each Pose normalized as 100% of maximum voluntary isometric contraction(MVIC). ResultsDolphin plank(DP) generated a very high-level muscle activation than 100% MVIC in all bilateral muscles, and there was a significant main effect of Pose e (p < 0.001). Erector spinae generated more activation in the Dolphin pose (p < 0.05) than in the HP and DS. No significant difference between bilateral activity differences in DP and DS. There was a significant difference between RRA and LRA (p < 0.001) and RIO and LIO (p < 0.0001). ConclusionFindings have suggested that the Dolphin plank could be used for abdomen strengthening, performance enhancement, and rehabilitation programs. Dolphin pose may benefit lower back stability by providing endurance to the Erector spinae muscle. So, this yoga pose can be incorporated into the yoga training module for addressing lower back pain(LBP) caused by longer sitting in a sedentary lifestyle.

Multidisciplinary Approach to Hypnosis: Psychiatric Basis, Neuroimaging and Genetic Findings

Hypnosis, a practice often misunderstood and surrounded by misconceptions, has a rich historical lineage dating back to ancient civilizations. Our review explores the relationship between neuroanatomy, genetics, and hypnotic susceptibility, investigating organic factors influencing an individual's responsiveness to hypnosis. This review highlights the importance of hypnosis as a high-level cognitive activity, especially in pain and anxiety management, and emphasizes the potential benefits of integrating hypnosis into healthcare practices. Recent advancements in neuroimaging have provided insights into the neurological mechanisms of hypnosis, while genetic research has expanded its applications. However, persistent misconceptions hinder its acceptance. This article offers a multidisciplinary basic exploration of hypnosis, focusing on its origins, historical development related to psychiatry, the basic neuroimaging findings mainly affecting the limbic system responsible for emotion, and genetic underpinnings. We aim to inspire clinicians, social scientists, and healthcare professionals to effectively integrate the scientific basics of hypnosis into therapeutic practice, contributing to a better understanding of its role in augmenting outcomes.

Automatic detection and interpretable analysis of learners’ cognitive states based on electroencephalogram signals

The development of higher-order thinking skills (HOTS) in learners is one of the educational objectives of the 21st century. Detecting learners’ higher and lower cognitive states based on electroencephalogram (EEG) signals is crucial for promoting the development of HOTS. In this study, we investigated the feasibility of using an EEG-based deep learning (DL) model to classify higher and lower cognitive states and employed eXplainable Artificial Intelligence (XAI) techniques to examine the neural mechanisms associated with the cognitive states. To trigger learners’ cognitive states, both high-level and low-level learning activities were developed based on a revised Bloom's taxonomy and the Interactive-Constructive-Active-Passive (ICAP) framework and used with 22 subjects whose EEG signal was recorded and detected with the Convolutional Neural Network-Long Short-Term Memory (CNN-LSTM) model. The CNN-LSTM model yielded a remarkable recognition accuracy of around 99 %. Finally, we proposed LIME-Brain Area (LIME-BA), an improvement of Local Interpretable Model-agnostic Explanation (LIME), to identify the distinctive attributes of brain area activities for different levels of cognitive states. According to the XAI interpretable analysis, we found that the frontal and temporal areas were activated in a higher cognitive state, and the occipital and parietal regions were activated in a lower cognitive state. This study provides further evidence for educators to design cognitive-guided instructional activities to enhance learners’ development of HOTS.

Technical vocational students’ higher learning institution preference and level of academic and skills preparedness

Vocational education is increasingly recognized for its role in creating gainful employment opportunities and stimulating economic growth. As such, this paper studied the higher learning institution preferences of the technical vocational students and their academic and skills readiness. Specifically, this study focused on the senior high school students enrolled in the technical and vocational livelihood track at Buruanga Vocational School and their regards on the growing importance of vocational education in job creation and national support. Utilizing a descriptive-correlational research design, this research examined the relationship between academic performance, skills preparedness, and higher learning institution preference 50 purposively selected completers as grounded on Vocational Identify Theory to understand how students perceived different career alternatives. Each student responded to self-report survey questionnaire that revealed academic preparedness and interest in various institutions. The results demonstrated a significant relationship between their readiness and preference for the institution. Additionally, the graduates indicated that their performance and literal understanding were lacking compared to their academic track. Hence, the outcomes confirmed that schools need to check their program levels and outreach and plan to offer higher-level services like the high school career guidance programs. The results provide hallmark-witted assortment preparations for strengthening undergraduates for high-level activities, thus preparing vocational schooling to attain a degree.

The effects of physical activity prescription on mental health: Evidence from primary care

While the benefits of physical activity on health are well documented, in high-income countries 1 in 3 adults do not reach the recommended levels. Thus, policy makers have developed interventions to promote physical activity. The aim of this research is to evaluate the effectiveness of physical activity prescription on mental health outcomes, by studying an intervention that prescribes physical activity at the primary care level in Catalonia (PAFES). This intervention specifically targets the adult population with high cardiovascular risk. We use data from the Health Survey of Catalonia (2011–2016) and exploit the variation in the number of trained General Practitioners that prescribe physical activity. Our results show that physical activity prescription reduces the probability of suffering from poor mental health. This effect is mainly driven by females within the targeted population. We also explore the main effect (or the output) of the intervention. While PAFES increases the probability of patients undertaking high-level physical activity, it does not affect rates of sedentarism or minutes walked per day. Results are consistent when using alternative mental health outcome measures, including self-reported depression and anxiety. We conclude that the prescription of physical activity not only contributes to the improvement of physical health but is also a useful tool to help preserve mental wellbeing.

Patellar Tendon Debridement and Reconstruction With Hamstring Tendon Autografts in the Setting of Severe Patellar Tendinopathy

Background: Patellar tendinopathy is a multifactorial condition characterized by micro-degenerative changes in the patellar tendon. This results from repeated stress to the knee extensor mechanism from explosive movement, axial malalignment, and/or abnormal patellar height. Currently, there is no standard surgical procedure for the treatment of severe patellar tendinopathy. Indications: Patellar tendon reconstructions for patellar tendinopathy may be indicated when &gt;50% of the tendon attachment is degenerative and detached from the distal patella and non-operative treatment, like physical therapy, bracing, and injections, have not relieved symptoms. Debridement may not offer long-term viability against augmentation of the patellar tendon with autografts, with a risk of recurrent breakdown of the patellar tendon attachment with resumption of previous activities that initially lead to overload. Technique Description: An anterior incision was made from the superior pole of the patella to the tibial tubercle. Then, dissection was performed medially to harvest the gracilis and semitendinosus tendons. Open harvesters were used, and the hamstring tendons were released proximally, keeping the tibial insertions attached. A horizontal pin was drilled across the tibial tubercle and reamed with a 4.5 mm reamer. A passing stitch was fed through the tunnel. The proximal and distal margins of the patella were localized with spinal needles and a pin was drilled across its midportion, 5 mm deep to the dorsal cortical margin, and then rearmed. Degenerative tissue at the patellar tendon was excised. The gracilis tendon was passed medial to lateral through the tibial tubercle, and a Q-Fix anchor anchored the grafts medially and laterally. The semitendinosus graft was passed medial to lateral through the patella followed by the gracilis tendon lateral to medial. Appropriate reconstruction graft tension was confirmed and grafts were whipstitched together. Results: Clinical outcomes for the debridement of severe patellar tendinopathy report 77% symptomatic relief. With many unable to return to activity, patellar tendon reconstruction can be indicated. Outcomes for reconstructions for severe patellar tendinopathy are not reported. Discussion: Patellar tendon reconstruction with hamstring autografts for the treatment of severe patellar tendinopathy can provide a strong reconstruction graft to allow for the resumption of high-level activities. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.

Cohort profile: the CARTaGENE Cohort Nutrition Study (Quebec, Canada)

PurposeTo address emerging nutritional epidemiological research questions, data from contemporary cohorts are needed. CARTaGENE is the largest ongoing prospective cohort study of men and women in Québec, Canada. Dietary information...

Comparison of short- and midterm outcomes in patients following ream-and-run and anatomic total shoulder arthroplasties

Anatomic total shoulder arthroplasty (aTSA) may not be an ideal treatment option for young and active patients because of potential activity restriction and concerns about glenoid loosening. The ream-and-run procedure (RnR) allows for the continuance of high-level activity without concerns of a glenoid component failure. Initial RnR publications are promising, although more outcomes studies are needed. Therefore, our primary purpose was to compare outcomes at multiple time points between matched aTSA and RnR cohorts. Second, we sought to examine relationships between patient-reported outcomes and preoperative glenoid pathoanatomy in our RnR cohort Last, we examined postoperative radiographs to determine if the RnR successfully corrected glenoid pathoanatomy and humeral head decentering. We performed a retrospective matched-cohort study comparing patients who underwent an RnR vs. patients who underwent the aTSA procedure between 2017 and 2019. All patients had primary diagnoses of shoulder osteoarthritis and a minimum of 2-year follow-up. Simple Shoulder Test, American Shoulder and Elbow Surgeons Standardized Shoulder Assessment Form (ASES), and daily and worst pain outcomes were compared between groups at 3 and 6 months, and 1 and 2 years postarthroplasty. Pre- and postoperative glenoid anatomy and humeral decentering were measured radiographically, and correlation analyses were conducted to explore relationships between these factors and 2-year pain and function scores. Forty-six shoulders (23 RnR and 23aTSA) belonging to 43 male patients with an average age of 56.2±8.3 years were included. Eighteen matched pairs were available at 3 and 6 months, 21 matched pairs at 1 year, and all 23 matched pairs at the 2-year time point. RnR patients reported significantly higher daily pain ratings (P=.047) and lower ASES scores (P=.031) compared with the aTSA group 3 months after arthroplasty but reported similar outcomes at 6 months and beyond. Preoperative pathoanatomy outcomes were not directly related to final reported pain or function in the RnR group. Additionally, the RnR was able to correct posterior humeral head decentering in our cohort. Young male patients undergoing RnR can likely expect similar short-term results as young male patients who undergo aTSA. Additionally, the RnR outcomes were not affected by preoperative glenoid wear or humeral head decentering. Our findings support the RnR as a viable surgical alternative for young, active patients with shoulder arthritis.

Acute aerobic exercise and creative thinking: comparison of transient hypofrontality state and strength model of self-controls’ theoretical predictions

Creativity is a high-level cognitive activity that can" be affected by acute aerobic exercise. This paper aims to determine how acute aerobic exercise intensities may influence aspects of divergent and convergent creative thinking. In this regard, to give an insight into the results, the predictions of the transient hypofrontality thesis and the strength model of self-control have been taken into consideration. Ninety-three healthy male college students (age = 21.18 ±1.73 years) were randomly assigned to anaerobic threshold intensity (%85 HRmax, n = 31), low intensity (%60 HRmax, n = 31), and control (n = 31) groups Participants performed divergent (Alternate Uses Task) and convergent (Raven's Advanced Progressive Matrices Test) creative thinking tasks before, during, and after cessation of two anaerobic thresholds and low intensities and control conditions. For the divergent creative thinking, flexibility and fluency performances during and after cessation of exercise in anaerobic threshold and low-intensity groups were improved in comparison to the control group.These results were consistent with the transient hypofrontality thesis and inconsistent with the strength model of self-control pre-dictions. In contrast, the performance of convergent creative thinking was relatively stable during and after cessation of exercise in anaerobic threshold and low-intensity groups which was inconsistent with both the transient hypofrontality thesis and the strength model of self-control predictions. The findings suggest that theoretical predictions of transient hypofrontality thesis are more adaptable with literature than the strength model of self-control to consider the exercise-creative processes relationship and further study of the issue would be of interest. Key words: Cognition, Convergent, Creativity, Divergent, Self-depletion, Physical Activity

The neurotransmitter calcitonin gene-related peptide shapes an immunosuppressive microenvironment in medullary thyroid cancer

Neurotransmitters are key modulators in neuro-immune circuits and have been linked to tumor progression. Medullary thyroid cancer (MTC), an aggressive neuroendocrine tumor, expresses neurotransmitter calcitonin gene-related peptide (CGRP), is insensitive to chemo- and radiotherapies, and the effectiveness of immunotherapies remains unknown. Thus, a comprehensive analysis of the tumor microenvironment would facilitate effective therapies and provide evidence on CGRP’s function outside the nervous system. Here, we compare the single-cell landscape of MTC and papillary thyroid cancer (PTC) and find that expression of CGRP in MTC is associated with dendritic cell (DC) abnormal development characterized by activation of cAMP related pathways and high levels of Kruppel Like Factor 2 (KLF2), correlated with an impaired activity of tumor infiltrating T cells. A CGRP receptor antagonist could offset CGRP detrimental impact on DC development in vitro. Our study provides insights of the MTC immunosuppressive microenvironment, and proposes CGRP receptor as a potential therapeutic target.

Characterization of the ddt1 Mutant in Rice and Its Impact on Plant Height Reduction and Water Use Efficiency.

Rice (Oryza sativa L.), a fundamental global staple, nourishes over half of the world's population. The identification of the ddt1 mutant in rice through EMS mutagenesis of the indica cultivar Shuhui527 revealed a dwarf phenotype, characterized by reduced plant height, smaller grain size, and decreased grain weight. Detailed phenotypic analysis and map-based cloning pinpointed the mutation to a single-base transversion in the LOC_Os03g04680 gene, encoding a cytochrome P450 enzyme, which results in a premature termination of the protein. Functional complementation tests confirmed LOC_Os03g04680 as the DDT1 gene responsible for the observed phenotype. We further demonstrated that the ddt1 mutation leads to significant alterations in gibberellic acid (GA) metabolism and signal transduction, evidenced by the differential expression of key GA-related genes such as OsGA20OX2, OsGA20OX3, and SLR1. The mutant also displayed enhanced drought tolerance, as indicated by higher survival rates, reduced water loss, and rapid stomatal closure under drought conditions. This increased drought resistance was linked to the mutant's improved antioxidant capacity, with elevated activities of antioxidant enzymes and higher expression levels of related genes. Our findings suggest that DDT1 plays a crucial role in regulating both plant height and drought stress responses. The potential for using gene editing of DDT1 to mitigate the dwarf phenotype while retaining improved drought resistance offers promising avenues for rice improvement.