High Hydrostatic Pressure Research Articles

Effects of high hydrostatic pressure treatment on in vitro digestibility and in vivo glycemic response of sweet potato flour: Effects of HHP on digestibility and glycemic response of sweet potato starch

Sweet potato is a rich source of resistant starch, fibre, vitamins and minerals, which is associated with healthy benefits. In this study, the effects of high hydrostatic pressure (HHP) treatment at 200–600 MPa on the digestibility of sweet potato flour (SPF) through starch composition analysis, in vitro digestion assays, and glycemic index (GI) and response in diabetic mice. Changes in the morphology of starch granules post-treatment were observed using an scanning electron microscope (SEM). Treatments at 400 and 600 MPa increased amylose in sweet potato starch and significantly increased the proportion of slowly digestible starch (SDS), as indicated by in vitro digestion assays. Resistant starch content decreased, with no significant change in rapidly digestible starch. Peak glucose levels in diabetic mice post-consuming SPF treated at 400 and 600 MPa were 476 and 514, respectively, significantly lower than 608 in the control group. The area under the curve (AUC) of 400 MPa treated-SPF is 14% lower than control SPF. The GI of SPF in the control group was 78.6, reducing to 66.2 and 68.3 post-HHP treatment at 400 and 600 MPa, respectively, with no significant difference in the 200 MPa group from that of the control group. SEM observations showed that damage to starch granules increased with increasing processing pressure. HHP caused the starch granules to gelatinize and recoagulate, forming an irregular shape. The HHP treatment changed the composition of SPF, significantly increasing SDS content and it stabilized postprandial blood glucose levels and reduced GI, rendering it a potential method to develop processed products of sweet potatoes with a low GI, providing a novel technical option for developing health-promoting products.

Changes in the microbiota and colour of pork meat batter during refrigerated storage in relation to the application of High Hydrostatic Pressure

The aim of this work was to study the effect of High Hydrostatic Pressures (HHP) on microbial degradation pattern and colour stability of pork meat batters used to produce fresh sausages. Besides treatments at 300 or 600 MPa for 5 min, pH (5.8 vs 5.4) and nitrite addition (100 vs. 0 mg/kg) were considered as variables. A not treated control was also monitored. The different samples were stored at 4°C and periodically analysed for their microbiota and colour. At the end of shelf life (TMC > 6.5 log CFU/g) a metagenomic analysis was also carried out to better clarify the effect of the treatments on microbial spoilage patterns. The results showed that the treatment at 300 MPa slightly affected initial cell load, but slowdown growth kinetics, especially at low pH, while 600 MPa strongly reduced microbial spoilage. The application of HHP was able to prolong shelf life of meat batters, from about two weeks (control) to 40 and 80 days in the case of treatments at 300 and 600 MPa, respectively. Metagenomic analysis revealed different degradation patterns in relation to tested variables: untreated samples showed the prevalence of Brochothrix spp., while the application of HHP determined the presence also of Carnobacterium, Bacillus, and Staphylococcus, depending on the treatment and the meat batter pH. Concerning colour, differences were mainly observed in redness (a*) and were due to the presence of nitrite. These findings suggest that HHP can be a feasible strategy to stabilize fresh sausages, although further studies are needed to assess their safety.

High hydrostatic pressure technology promotes the formation of lotus seed starch and epigallocatechin gallate complex: An evaluation of structure and digestive characteristics

High hydrostatic pressure technology promotes the formation of lotus seed starch and epigallocatechin gallate complex: An evaluation of structure and digestive characteristics

Exploring robustness of hybrid membranes under high hydrostatic pressure and temperature

Bacterial membranes are typically composed of ester-bonded fatty acid (FA), while archaeal membranes consist of ether-bonded isoprenoids, differentiation referred to as the ‘lipid divide’. Some exceptions to this rule are bacteria harboring ether-bonded membrane lipids. Previous research engineered the bacterium Escherichia coli to synthesize archaeal isoprenoid-based ether-bonded lipids together with the bacterial FA ester-linked lipids, showing that heterochiral membranes are stable and more robust to temperature, cold shock, and solvents. However, the impact of ether-bonded lipids, either bacterial or archaeal, on membrane robustness, remains unclear. Here, we investigated the robustness, as survival after shock, of E. coli synthesizing either archaeal or bacterial ether-bonded membrane lipids, under high temperature and/or high hydrostatic pressure (HHP). Our findings reveal E. coli with bacterial ether-bonded lipids is more robust under HHP and high temperature. On the contrary, the presence of archaeal ether-bonded membrane lipids in E. coli does not affect the robustness under HHP nor high temperature under the tested conditions. We observed morphological changes induced by the shock treatments including reduced length under high temperature or HHP, and the presence of elongated cells after a shock of HHP and high temperature combined, suggesting the combined treatments impaired cell division. Our results contribute to a deeper understanding of membrane adaptation to extreme environmental conditions and highlight the significance of HHP as a key parameter to investigate the differentiation of membranes during the lipid divide.

Impact of High Hydrostatic Pressure and UV-C Radiation on Total Aerobic Bacteria in Minas Frescal Cheese.

Minas Frescal cheese (MFC) is a perishable product with high water activity and neutral pH, conditions that favor the development of microorganisms. Total aerobic bacteria (TAB) can deteriorate the cheese, negatively affecting its sensory characteristics. By controlling TAB, the shelf life of the product is extended and its quality is maintained, contributing to meeting consumer expectations. This study aimed to evaluate the individual and combined effect of technological treatments of high hydrostatic pressure (HHP) and ultraviolet C radiation (UV-C) on the TAB count present in the natural microbiota of MFC, as an alternative to control the microbiological quality of this product. After production, MFC were subjected to treatments with different levels of HHP (100 to 400 MPa/10 min) and UV-C (0.097 to 0.392 J/cm2.s-1). The combinations of HHP and UV-C doses were determined by a central composite rotational design (CCRD). The model efficiently described the individual and combined effect of HHP and UV-C on TAB, demonstrating that gradually increasing HHP levels reduces TAB counts in MFC, regardless of the UV-C dose applied. This study contributes significantly to the literature by providing new insights into how these technologies can be used to improve the microbiological quality of fresh cheeses.

Pressure-tolerant survival mechanism of Schizophyllum commune 20R-7-F01 isolated from deep sediments 2 kilometers below the seafloor

In anaerobic high hydrostatic pressure (HHP) sedimentary environments below the seafloor, fungi are found to dominate the eukaryotic communities, playing crucial ecological roles. However, the specific mechanisms by which fungi adapt to anaerobic HHP environments remain unclear. In this study, we investigated Schizophyllum commune 20R-7-F01 isolated from coal-bearing sediments at a depth of 2 km below the seafloor. By assessing the cell viability, biomass, and cell wall thickness changes of strain 20-7-1 under different HHP conditions, we observed that, compared to 0.1 MPa, strain 20-7-1 exhibited slower growth rates and decreased cell viability at 15 MPa and 35 MPa, yet demonstrated significant pressure tolerance. Transcriptomic and metabolomic analyses revealed that this strain activated the carbohydrate metabolic process to simultaneously utilize ethanol and lactic acid fermentation pathway. Additionally, it activates the oxidoreductase activity and hydrolase activity pathways to detoxify intracellular reactive oxygen species (ROS). Activation of the metal ion binding pathway increases the proportion of unsaturated fatty acids in the cell membrane, while instigation of the integral component of membrane pathway maintains cell wall structural stability. Furthermore, activation of the DNA repair pathway repairs DNA damage, demonstrating its comprehensive adaptive mechanisms against the HHP stress. These research findings deepen our understanding of fungal survival strategies and adaptation mechanisms in extreme environments, laying the groundwork for further exploration of their roles in cycling of carbon, nitrogen, sulfur, and other elements in the deep biosphere.

Solvent- and Catalyst-Free Environmentally Benign High Hydrostatic Pressure-Assisted Synthesis of Bioactive Hydrazones and the Evaluation of Their Stability Under Various Storage Conditions

Our group has seen great promise in using substituted diaryl-hydrazones to alleviate oxidative stress in preeclampsia. Specifically, fluorinated diaryl-hydrazones have shown great efficacy, confirmed via antioxidant assays and animal trials using pregnant mice. In addition to efficient antioxidant properties, these diaryl-hydrazones are also considered non-toxic. While the synthesis of these compounds is relatively simple, it commonly utilizes undesirable solvents and glacial acetic acid as the catalyst; additional solvents are needed for the isolation of the desired products, which negatively affects the green synthesis of the hydrazones. To combat this possible industrial roadblock, we have begun incorporating the use of hydrostatic high pressure (HHP) in the synthesis. The use of HHP allowed us to synthesize substituted diaryl-hydrazones in a 1:1 molar ratio without the need for solvents or acid catalysts. The optimized procedure can produce nearly quantitative yields, leading to an easier isolation of the products. Different HHP methodologies, such as constant high-pressure treatment and cycling (with different number of cycles, holding and decompression times) were applied and cycling was observed to be the most efficient activation for the majority of the reactions. Stability experiments were also conducted with one of the products and observed that although the solid-state storage does not alter the hydrazone, storing it in various solvents may significantly decrease the concentration of the active component which should be considered when performing the biochemical/biological assays.

Sea Buckthorn Flavonoid Extracted with High Hydrostatic Pressure Alleviated Shrimp Allergy in Mice through the Microbiota and Metabolism.

Sea buckthorn (Hippophaë rhamnoides L.) known as the deciduous shrub has been reported to have effects of antioxidant, anti-inflammatory, and immunomodulatory activities. Tropomyosin (TM) induced a regulatory immune response associated with food allergy. In this study, a mouse model of food allergy sensitized to tropomyosin (TM) was established to assess the antiallergic properties of sea buckthorn flavonoid extract (SBF). SBF alleviated mice's allergic symptoms and exhibited a significant reduction in the levels of IgE and histamine. Meanwhile, SBF repaired the allergic Th2 cell overpolarization generated by TM, via downregulating the IL-4 production and upregulating IFN-γ production to restore the balance of Th1/Th2 cells. Furthermore, the 16S RNA analysis showed that SBF primarily restored the gut microbiota via increasing the abundance in Chitinophilidae and decreasing in Burkholderiaceae, Pneumatobacteriaceae, and Sphingomonadaceae. Gut metabolomes determined by liquid chromatography-mass spectrometry (LC-MS) suggested that TM upregulated PE (14:0/22:1(13Z)) and SBF decreased formimino-l-glutamic acid and urocanic acid levels. According to the KEGG pathway analysis, SBF treatment has been shown to modulate glycerophospholipid and histidine metabolism to improve allergic reactions. SBF holds great promise as a novel potential agent for the treatment of food allergies.

Local measurement on oedometric compression tests: Time and temperature ageing effects on a fluorosilicone

O-ring durability is a key issue for engineering structures that require sealing over a long service life. Rubber-like materials are used for this type of component because of the assumption of incompressibility, i.e. a high bulk modulus K. However, this assumption has been called into question in the literature, particularly for elastomers under high hydrostatic pressures. This work examines the compressibility of rubber-like materials, with a particular focus on fluorosilicone elastomers (FVMQ). A method of confined compression using a transparent crucible is presented, which allows local measurement of the displacement field. This technique provides a better understanding of the analysis of oedometric compression data and a reliable way to determine the K value. Furthermore, this approach allows following the evolution of K over time with accelerated ageing. Three ageing temperatures - 200, 220 and 250 °C - were tested up to 34, 15 and 1 weeks respectively. For the three ageing temperatures, the FVMQ results show a decrease in K values with ageing. In particular, at 250 °C, a turning point was observed after 72 h of ageing. These results highlight the influence of ageing on the compressibility of the FVMQ and the presence of two different ageing mechanisms affecting the K evolution.

Effects of high hydrostatic pressure on folate and isoflavone contents, bioaccessibility, and physicochemical properties of soybean powder

Effects of high hydrostatic pressure on folate and isoflavone contents, bioaccessibility, and physicochemical properties of soybean powder

Phase-Changeable Metafabric Enables Dynamic Subambient Humidity and Thermal Regulation.

A promising approach to prevent heat- and cold-related illnesses is the integration of zero-energy input control technology into personal thermal management (PTM) systems while reducing energy consumption. However, achieving optimal wearing comfort while maintaining subambient metabolic temperatures using thermally regulating materials without an energy supply remains challenging. In this study, we provide a simple and reliable methodology to produce a phase-changeable metafabric made of thermoplastic polyurethane and phase change capsule (PCC) particles with high moisture permeability and thermal comfort. This approach skillfully incorporates spray-formed PCC particles into a three-dimensional nanofibrous aggregate, forming a stable self-entangled network structure in a single step through simultaneous humidity-assisted electrospraying and electrospinning processes. Additionally, the metafabric demonstrates prominent water resistance and superhydrophobicity, which are attributed to the integration of PCC particles and nanofibers, resulting in the formation of a microporous/nanoporous structure resembling the surface of a lotus leaf. As a result, the phase-changeable metafabric shows an active and passive thermal control performance, with a water vapor transmittance rate of 13.1 kg m-2 d-1 and a phase change enthalpy of 115.05 J g-1 even after 100 thermal cycles. Furthermore, it displays excellent waterproofing capability, characterized by a water contact angle of 158.7° and the ability to withstand a high hydrostatic pressure of 87 kPa. In addition, the metafabric exhibits a good mechanical performance, boasting a tensile strength of 10.5 MPa. Overall, the proposed economical metafabric is an exemplary candidate material for next-generation PTM systems.

Comparative study of lysine acetylation in Vesicomyidae clam Archivesica marissinica and the manila clam Ruditapes philippinarum: adaptation mechanisms in cold seep environments

BackgroundThe deep-sea cold seep zone is characterized by high pressure, low temperature, darkness, and oligotrophy. Vesicomyidae clams are the dominant species within this environment, often forming symbiotic relationships with chemosynthetic microbes. Understanding the mechanisms by which Vesicomyidae clams adapt to the cold seep environment is significant. Acetylation modification of lysine is known to play a crucial role in various metabolic processes. Consequently, investigating the role of lysine acetylation in the adaptation of Vesicomyidae clams to deep-sea environments is worthwhile. So, a comparative study of lysine acetylation in cold seep clam Archivesica marissinica and shallow water shellfish Ruditapes philippinarum was conducted.ResultsA total of 539 acetylated proteins were identified with 1634 acetylation sites. Conservative motif enrichment analysis revealed that the motifs -KacR-, -KacT-, and -KacF- were the most conserved. Subsequent gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses were conducted on significantly differentially expressed acetylated proteins. The GO enrichment analysis indicated that acetylated proteins are crucial in various biological processes, including cellular response to stimulation, and other cellular processes ( p < 0.05 and false discovery rate (FDR) < 0.25). The results of KEGG enrichment analysis indicated that acetylated proteins are involved in various cellular processes, including tight junction, motor proteins, gap junction, phagosome, cGMP-PKG signaling pathways, endocytosis, glycolysis/gluconeogenesis, among others (p < 0.05 and FDR < 0.25). Notably, a high abundance of lysine acetylation was observed in the glycolysis/glycogenesis pathways, and the acetylation of glyceraldehyde 3-phosphate dehydrogenase might facilitate ATP production. Subsequent investigation into acetylation modifications associated with deep-sea adaptation revealed the specific identification of key acetylated proteins. Among these, the adaptation of cold seep clam hemoglobin and heat shock protein to high hydrostatic pressure and low temperature might involve an increase in acetylation levels. Acetylation of arginine kinase might be related to ATP production and interaction with symbiotic bacteria. Myosin heavy chain (Ama01085) has the most acetylation sites and might improve the actomyosin system stability through acetylation. Further validation is required for the acetylation modification from Vesicomyidae clams.ConclusionA novel comparative analysis was undertaken to investigate the acetylation of lysine in Vesicomyidae clams, yielding novel insights into the regulatory role of lysine acetylation in deep-sea organisms. The findings present many potential proteins for further exploration of acetylation functions in cold seep clams and other deep-sea mollusks.

Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments

Fungi inhabiting deep subseafloor sediments have been shown to possess anaerobic methane (CH4) production capabilities under atmospheric conditions. However, their ability to produce CH4 under in situ conditions with high hydrostatic pressure (HHP) remains unclear. Here, Schizophyllum commune 20R-7-F01, isolated from ~2 km below the seafloor, was cultured in Seawater Medium (SM) in culture bottles fitted with sterile syringes for pressure equilibration. Subsequently, these culture bottles were transferred into 1 L stainless steel pressure vessels at 30 °C for 5 days to simulate in situ HHP and anaerobic environments. Our comprehensive analysis of bioactivity, biomass, and transcriptomics revealed that the S. commune not only survived but significantly enhanced CH4 production, reaching approximately 2.5 times higher levels under 35 MPa HHP compared to 0.1 MPa standard atmospheric pressure. Pathways associated with carbohydrate metabolism, methylation, hydrolase activity, cysteine and methionine metabolism, and oxidoreductase activity were notably activated under HHP. Specifically, key genes involved in fungal anaerobic CH4 synthesis, including methyltransferase mct1 and dehalogenase dh3, were upregulated 7.9- and 12.5-fold, respectively, under HHP. Enhanced CH4 production under HHP was primarily attributed to oxidative stress induced by pressure, supported by intracellular reactive oxygen species (ROS) levels and comparative treatments with cadmium chloride and hydrogen peroxide. These results may provide a strong theoretical basis and practical guidance for future studies on the contribution of fungi to global CH4 flux.

Origin of Pressure Resistance in Deep-Sea Lactate Dehydrogenase.

High hydrostatic pressure has a dramatic effect on biochemical systems, as exposure to high pressure can result in structural perturbations ranging from dissociation of protein complexes to complete denaturation. The deep ocean presents an interesting paradox since it is teeming with life despite the high-pressure environment. This is due to evolutionary adaptations in deep-sea organisms, such as amino acid substitutions in their proteins, which aid in resisting the denaturing effects of pressure. However, the physicochemical mechanism by which these substitutions can induce pressure resistance remains unknown. Here, we use molecular dynamics simulations to study pressure-adapted lactate dehydrogenase from the deep-sea abyssal grenadier (Coryphaenoides armatus), in comparison with that of the shallow-water Atlantic cod (Gadus morhua). We examined structural, thermodynamic and volumetric contributions to pressure resistance, and report that the amino acid substitutions result in a decrease in volume of the deep-sea protein accompanied by a decrease in thermodynamic stability of the native protein. Our simulations at high pressure also suggest that differences in compressibility may be important for understanding pressure resistance in deep-sea proteins.

Author Correction: Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

Author Correction: Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

Enhanced antioxidant activities, polyphenols, flavonoids, and free amino acid contents of Nelumbo nucifera bee pollen via high hydrostatic pressure treatment

ABSTRACT The aim of the present study was to address the effect of high hydrostatic pressure (HHP) on bioactive compounds and antioxidant activity of Nelumbo nucifera (Lotus) bee pollen (LBP). The total phenolic contents (TPC) and free amino acids were determined and flavonoid composition was identified by UPLC-Triple-TOF-MS. Five analytical methods including DPPH, ABTS, reducing power, FRAP, and silver nanoparticles antioxidant capacity were used for the antioxidant activity analysis. The results revealed a significant increase in the TPC and free amino acid compositions after HHP treatment. The DPPH, ABTS, reducing power, FRAP, and AgNPAC of the extracted fractions were augmented by over 13.94, 2.9, 2.68, 85, and 2.6 times, respectively. PCA analysis showed a strong correlation between TPC, some distinct flavonoids, and antioxidant activities. These results indicated that HHP enhances the antioxidant activity of LBP via increase in amino acids, TPC, and some flavonoids such as Licoagrone, Kuwanon K, Cyanidin 3-rutinoside, etc.

Modification of pepper seed protein isolate to improve its functional characteristic by high hydrostatic pressure

Pepper seed protein isolate (PSPI) is a valuable plant-based protein source, yet the impact of processing methods such as high hydrostatic pressure (HHP) on its properties remains unclear. The impact of HHP on the structural and functional properties of PSPI at pH 7 and pH 9 was evaluated. Structural changes in PSPI were analyzed using spectral techniques, revealing significant alterations in the secondary and tertiary structures induced by HHP treatment. HHP treatment caused the unfolding of the PSPI structure, leading to the exposure of previously hidden chromophores and hydrophobic groups. The treatment also led to changes in free sulfhydryl groups and increased average particle size suggesting the formation of macromolecular polymers or insoluble aggregates. Consequently, the water-holding capacity, oil-holding capacity, foaming characteristics, and emulsifying activity index of the modified PSPI were significantly enhanced both at pH 7 and pH 9, with maximum improvements of 121.98 %, 157.29 %, 100.00 %, and 265.78 %, respectively. In conclusion, HHP is a promising strategy for enhancing the physicochemical properties of PSPI for various applications.

Determining the effect of high hydrostatic pressure on the refrigerated stability of avocado puree

The present study aimed to extend refrigerated stability of locally grown avocado fruit by applying High Hydrostatic Pressure (HHP) treatment. HHP was applied at 600 MPa for 3 min to avocado puree and stored at 4 °C for 28 days. Physicochemical (colour CIE L*, a*, b*, total oil amount, chlorophyll, pH, moisture) and microbiological (mesophilic, yeast-mould) analyses were performed at seven-day intervals on both control (untreated) and HHP-treated sample packs. It could be judged that HHP treatment effectively controlled the colour indices, preventing undesired changes during the cold storage period of avocado puree. In conclusion, this study demonstrated that HHP-processed avocado puree exhibited stability for 28 days compared to unprocessed puree at 4 °C. The shelf-life stability of avocado puree under chilled conditions was extended from 7 to 28 days.Graphical

High hydrostatic pressure processing of whole buckwheat grains to obtain functional gluten-free ingredients: Effect of pressure and holding time

High Hydrostatic pressure (HHP) represents an environmental-friendly and efficient non-thermal technology capable of inducing changes that can lead to functional improvements on starchy materials preserving the nutritional value of the original flours. The development of gluten-free (GF) flours and ingredients remain a challenge due to the need to improve the nutritional and techno-functional aspects of these commodities. Pre-soaked whole buckwheat (BW) grains were subjected to HHP treatment under different processing conditions: two pressure levels (300–600 MPa) and three holding times (0-5-15 min) to evaluate the techno-functional and nutritional response of the resulting flours. Significant (p < 0.05) increases in water absorption capacity and swelling capacity were observed in the flours resulting from longer treatment times (5–15 min) and the highest-pressure level (600 MPa). Higher thermal stability was also observed in the pasting profiles obtained for these flours. Flours resulting from HHP treatments at 600 MPa for 15 min also showed higher antioxidant capacity (DPPH and ORAC). The resulting flour from these processing conditions was used at different concentrations (15, 30, 50, and 70 %) in a GF bread formulation. An increase in the elastic modulus values of the doughs, the specific volume of the resulting bread and a reduction in the crumb firmness were observed in the formulations containing the BW-modified flour compared to the counterparts made with a native flour. Therefore, it can be concluded that the HHP treatment of whole buckwheat grains represents an interesting alternative for the development of ingredients with suitable technological and nutritional properties for gluten-free bakery formulations.

Modulating the techno-functional properties of quinoa (Chenopodium quinoa Wild) protein concentrate using high-pressure technologies and their impact on in vitro digestibility: A comparative study

High hydrostatic pressure (HHP; 200–600 MPa at 24 °C for 20 min) and dynamic high pressure (DHP; 50–180 MPa) were applied to modulate the techno-functional properties of quinoa protein concentrate (QPC) produced from non-defatted flour using a lab-scale extraction. QPC's unique composition, with 74.6 % protein and significant levels of unsaturated fatty acids, influenced the effects of HHP and DHP. HHP significantly (P < 0.05) enhanced hydration properties, peaking at 400 and 500 MPa (on average 4.34 g/g), while DHP reduced the oil absorption capacity from 2.5 to 1.8 g/g. Both processing techniques decreased QPC protein solubility. This reduction corresponded to decreased foaming capacity and emulsifying properties in HHP-processed QPC. DHP increased emulsifying activity and stability indices, with optimized improvements at 100 MPa of 39.4 and 414.4 %, respectively. These modifications in QPC's properties occurred without noticeable loss in in vitro protein digestibility (IVPDaverage = 86.1 %). The findings support the potential of high-pressure technologies to uniquely modulate different techno-functional properties of QPC, produced from non-defatted flour, while maintaining high digestibility, thereby offering greater versatility in its use. Industrial relevanceQuinoa is a strategic choice for diversifying plant protein sources, with the use of non-defatted flour in QPC production offering new insights into its composition and properties, reducing both costs and environmental impact. In this study, HHP and DHP, recognized as “green” physical processing technologies, demonstrated advantageous potential to develop innovative quinoa protein ingredients with added value and clean-label appeal, while maintaining their high nutritional value. The distinctive effects of high-pressure technologies and varying pressure levels on modulating QPC's techno-functional properties underscore the creation of multifunctional QPC and the optimization of key properties, addressing the urgent demands of the plant-based food segment.