High Fertility Research Articles

Effect of Black Soldier Fly Larvae Frass as Organic Fertilizer on Postharvest Quality and Shelf Life of Open-field-grown Tomato (Solanum lycopersicon L.)

Open-field-grown tomatoes in Saudi Arabia are stressed by poor soil fertility because of the low organic matter content, low nutrient availability, and high pH. Thus, high chemical fertilizer inputs are crucial for commercial production; however, they cause economic loss as well as negatively affect environmental sustainability. The use of efficient organic fertilizers would be useful for developing more cost-effective, environmentally friendly, and sustainable agriculture. The current study evaluated the impact of Black soldier fly (Hermetia illucens L.) frass (BSFF) fertilizer applications as organic fertilizer on postharvest fruit quality and shelf life of open-field-grown F1 hybrid ‘Sinag Tala’ tomatoes compared with those grown under control treatment (received recommended doses of chemical fertilizers for tomato production under open field conditions). The experiment was conducted using a randomized complete block design with four replicates. The results showed that the application of BSFF fertilizer, especially at the higher rate (5 ton/ha; BSFF3), improved the overall quality of tomato fruit. The BSFF fertilizer treatments reduced weight loss, maintained higher firmness, antioxidants [vitamin C, total phenol content (TPC) and total flavinoid (TFC)], total soluble solids (TSS), and titratable acidity (TA) contents, and lowered the TSS/TA ratio of fruits during shelf life compared with those of the control. The decay incidence was only detected after 9 days of shelf life and was significantly lower in all the BSFF fertilizer treatments (range, 8.40%-12.05%) than in the control (15.9%). In addition, BSFF fertilizer treatments reduced both disease incidence and severity of gray mold following pathogen inoculation during shelf life compared with those of the control. These results might be attributable to the higher antioxidants content (vitamin C, TPC, and TFC) and higher polyphenol oxidase activity in BSFF-fertilized tomatoes. In conclusion, BSFF fertilizer could be used as a potential eco-friendly alternative to chemical fertilizers to improve tomato fruit quality during shelf life.

The Secondary Sex Ratio and Male Mortality at Pre-Reproductive Ages: A Test of Selection In Utero.

The secondary sex ratio (i.e., the ratio of male to female live births; hereafter referred to as the SSR) falls in populations encountering ambient stressors. Much theory and some empirical work indicates that males born to low SSR cohorts may be "positively selected" in that excess culling in utero may correspond with greater than expected survival among live-born males in that cohort. We extend prior work by testing, in historical Utah, whether the SSR varies positively with male mortality at pre-reproductive ages. This study uses detailed records from the Utah Population Database to focus on Utahns born 1850-1940. We use rigorous time-series methods, which control for strong secular declines in mortality as well as ambient perturbations shared equally among males and females, to investigate the male culling inference. We observe a positive relation between the SSR and male mortality during youth (i.e., 5 to < 20 years; p < 0.05) but not in infancy or early childhood. In this historical population, the SSR appears to gauge hardiness of surviving male cohorts. However, whether the high fertility and/or family structure context of Latter-day Saints in historical Utah explains the age-specific pattern of male mortality warrants further scrutiny.

Genetic Diversity of Maize (Zea mays L.) Genotypes under Low Level of Nitrogen

Maize (Zea mays L.) is India's second most significant crop, behind rice. As a cross-pollinated crop, hybrid vigour is commonly used to achieve the best production potential. The selection of various genotypes is a primary goal in the maize hybrid breeding programme. Maize is a voracious feeder and requires a lot of fertiliser. The biggest limits on maize production in India are low soil nitrogen and high fertiliser costs. Jharkhand consumes only 82.5 kg of total fertiliser nutrients per acre. In order to discover genetically varied maize genotypes for nitrogen usage efficiency, the current study evaluated 121 maize genotypes in a randomised block design with two replications at low nitrogen levels. Plant height, days to 50% pollen shed, days to 50% silk, anthesis-silking interval (ASI), days to75% maturity, number of leaves per plant, root length, stover yield per plant, grain yield per plant, and harvest index were all recorded. Statistical analysis was performed using the morphological trait data. Analysis of variance revealed substantial (p≤0.05) variations in morphological features between maize genotypes. For statistical analysis, a clustering method was used to locate various genotypes with greater genetic distance in order to develop good heterotic combinations. One hundred twenty one genotypes were divided into six clusters using the dendogram-by-ward minimum variance approach. Cluster III and V had the greatest inter-cluster distance (79.26), followed by cluster III and VI (74.77) and cluster II and III (73.80). The many heterotic combinations developed from these clusters can be employed in subsequent breeding programmes to produce low nitrogen-requiring hybrids with a low yield penalty.

Biological Characterization of Geo-Referenced Soil Samples from Major Rice-Growing Tracts of Southern Kerala

A survey was conducted from January to March 2023 to assess the soil biological fertility index across five agro-ecological units in the rice-growing belts of Southern Kerala. These units included Onattukara sandy loam (AEU 3), Kari soil (AEU 4), Pokkali soil (AEU 5), Southern laterites (AEU 8), and Midland laterites (AEU 9). The study was carried out in the College of Agriculture, Vellayani Thiruvananthapuram. The survey aimed to assess microbial activity and organic carbon content, thereby enhancing our understanding of the biological fertility across these diverse soil types. Twenty geo-referenced surface soil samples @ 0- 15 cm were collected in every AEU by prescribed method. The collected samples were subjected to the characterization of soil biological properties such as dehydrogenase activity, organic carbon, microbial biomass C, microbial biomass N and soil respiratory rate following the standard protocol. The findings showed that organic carbon in AEU 5 with the highest mean value (3.03 %), followed by AEU 4 (2.19 %), AEU 9 (2.01 %), AEU 8 (1.01 %), and AEU 3 (0.56 %). Dehydrogenase activity was highest in AEU 5 (682.54 µg TPF g-1 hr-1), followed by AEU 4, AEU 9, and AEU 8, and lowest in AEU 3 (76.71 µg TPF g-1 hr-1). AEU 5 exhibited the highest microbial biomass carbon and microbial biomass nitrogen activities, while AEU 8 showed the lowest. Soil respiration was highest in AEU 5 and lowest in AEU 3. Based on these soil microbiological indicators, soils were classified into three categories: low, medium, and high fertility. The Biological Fertility Index (BFI) scores indicated favorable conditions in AEU 4 and 5, moderate in AEU 9, and low in AEU 3 and 8. By establishing a comprehensive understanding of soil biological fertility across various agro-ecological units, the research aims to contribute to improved soil health and crop productivity in the region.

The Residual Activity of Fatty Acyl-CoA Reductase Underlies Thermo-Sensitive Genic Male Sterility in Rice.

Photoperiod/thermo-sensitive genic male sterility (P/TGMS) is critical for rice two-line hybrid system. Previous studies showed that slow development of pollen is a general mechanism for sterility-to-fertility conversion of TGMS in Arabidopsis. However, whether this mechanism still exists in rice is unknown. Here, we identified a novel rice TGMS line, ostms16, which exhibits abnormal pollen exine under high temperature and fertility restoration under low temperature. In mutant, a single base mutation of OsTMS16, a fatty acyl-CoA reductase (FAR), reduced its enzyme activity, leading to defective pollen wall. Under high temperature, the mOsTMS16M549I couldn't provide sufficient protection for the microspores. Under low temperature, the enzyme activity of mOsTMS16M549I is closer to that of OsTMS16, so that the imperfect exine could still protect microspore development. These results indicated whether the residual enzyme activity in mutant could meet the requirement in different temperature is a determinant factor for fertility conversion of P/TGMS lines. Additionally, we previously found that res2, the mutant of a polygalacturonase for tetrad pectin wall degradation, restored multiple TGMS lines in Arabidopsis. In this study, we proved that the osres2 in rice restored the fertility of ostms16, indicating the slow development is also suitable for the fertility restoration in rice.

Unlocking Potential: The Role of Zinc Fortification Combating Hidden Hunger and Enhancing Nutritional Security

Micronutrient shortage is rapidly becoming apparent have drawn more attention in the cultivation of crops. The main causes of this deficit are the introduction of high-yielding varieties, an intensified cropping strategy, and advanced irrigation systems etc. A further aspect contributing to this issue is the increased use of high analysis chemical fertilizers instead of organic plant nutrition (composts, farmyard manure, etc.). Most countries have acute shortages of micronutrients due to the significant depletion of soil reserves caused by current agricultural production technologies. In order to increase both the quantity and quality of crops, micronutrients are crucial. Through the integration of agronomic, breeding and transgenic techniques, researchers seek to strengthen the zinc concentration in field crops, so improving their nutritional value and mitigating the risk of zinc deficiency in human diets. The availability and absorption of micronutrients in crops are improved by agronomic techniques such as foliar spraying, and soil fertilizer treatment including organic amendments. Meanwhile, biofortification of vegetable and fruit crops has also been achieved by transgenic and breeding strategies. In other hand, Rhizobacteria-based biofortification, Chelated Zn biofortification, nutri-priming are also important techniques in Zinc fortification programs to ensure food security and nutritional quality, bio-fortification of micronutrients in crops is vital. In addition, bio-fortification improved quality and crop output, reducing hidden hunger and demonstrating that it was a viable and economical approach. The present review addresses several aspects of zinc insufficiency in human populations, including public health and socioeconomic issues, bio-fortification and ferti-fortification studies, and future efforts to mitigate zinc deficiency in soil and the population at large.

Promoting Male Involvement in Family Planning: Insights From the No-Scalpel Vasectomy Program of Davao City, Philippines.

Despite global consensus on the importance of male involvement in family planning, disparities persist in low- and middle-income countries, where women continue to bear the responsibility for these initiatives. The Philippines, with a high fertility rate and unmet family planning needs, exemplifies this challenge. We present the experiences and lessons learned from implementing the no-scalpel vasectomy (NSV) program in Davao City, showcasing its potential for increasing male engagement in family planning decisions. Launched in 2008, the program aimed to address gender disparity by promoting NSV as a safe and effective contraceptive alternative to female-centric methods. Through the use of culturally sensitive information campaigns and couple-focused counseling, the program challenged traditional notions of masculinity and encouraged shared decision-making. Strong local government commitment and public-private partnerships played key roles in driving the program's success. Results showed an average annual increase of 80% in NSV clients over the past 3 years compared to before the COVID-19 pandemic, underscoring its effectiveness. The program presents a compelling intervention model for similar initiatives, highlighting how overcoming cultural barriers, infrastructure limitations, and budgetary constraints through policy advocacy, strategic partnerships, and tailored approaches can significantly boost male involvement in family planning and improve reproductive health outcomes within communities.

Nutrient circularity of an anaerobic digestion project in a typical intensive rice cultivation village in the Vietnamese Mekong delta region: a regional material flow analysis and discussion of modeling practices

AbstractRice is the third largest agricultural commodity in the world. Intensive rice production is associated with high fertilizer application, and vast quantities of rice straw are often disposed of by open burning. As emissions of N and P from fertilizer and their impacts are highly spatially disparate, it is crucial to locally assess potential solutions for nutrient circularity. A model framework was established to evaluate the potential impact of local N, P and C cycles along with suitable circularity indicators. This framework was applied for a case study in the typical intensive rice cultivation village Hậu Mỹ Bắc B in the Mekong Delta using STAN modeling software. A novel technique, based on a patented temperature-phased anaerobic digestion (TPAD) loop-reactor design was investigated as a measure to reduce rice straw open burning, generate bioenergy and recover digestate for nutrient recirculation. The case study showed that rice straw TPAD could reduce open burning by 60%, while the N recovery rate increased to 21% when 200 ± 34 Mg/a of inorganic fertilizer was replaced with digestate. For C and P, the recovery rates increased to 14% and 11%, respectively. Based on the results, we concluded that TPAD has a strong impact on local nutrient circularity in terms of recirculation and sufficiency. Furthermore, the developed framework is suitable to use for master planning of nutrient circularity and for technological impact assessments at local scale. However, versatile, dynamic, and comparable monitoring frameworks with harmonized indicators to identify options for nutrient circularity are required for the detailed modeling of regional nutrient management.

Performance of different wheat varieties and their associated microbiome under contrasting tillage and fertilization intensities: Insights from a Swiss long-term field experiment

Winter wheat is an important global cereal crop. However, conventional farming practices, characterised by intensive tillage and high fertilizer inputs, pose significant threats to the environment. In response, more conservative management practices are being applied aiming to maintain wheat production while promoting a beneficial microbiome. Here, we evaluated the suitability of three different wheat varieties for less intensive agricultural systems, focusing on reduced tillage and fertilizer intensity. The study was conducted over two consecutive years in a Swiss long-term field experiment comparing conventional versus reduced tillage and full fertilization versus half fertilization. In addition, we investigated the composition of plant-associated microbial communities using amplicon sequencing of phylogenetic marker genes, specifically targeting bacteria and fungi in rhizosphere samples and fungi in root samples. Our results revealed that in our study wheat variety most strongly predicted grain yield and quality, independent of tillage and fertilization intensity. Specifically, wheat varieties demonstrated higher yields and N uptake in plots subjected to conventional ploughing and full fertilization compared to those under reduced tillage and half fertilization. We found no significant effect of wheat variety on the composition of microbial communities. However, tillage emerged as the primary factor influencing microbial community composition in the rhizosphere, while fertilization intensity significantly impacted fungal communities in the root system. These findings underscore the complex interplay between agronomic practices, plant genetics, and microbial dynamics in agroecosystems, emphasizing the need for holistic and adaptive approaches and their further development to ensure sustainable crop production.

Comparative study of the reproductive activity of Calliptamus barbarus (Costa, 1839) and Calliptamus wattenwylianus (Pantel, 1836) in a subhumid bioclimatic region

The reproductive activity of Calliptamus barbarus and Calliptamus wattenwylianus was investigated to understand population dynamics and reproductive potential in a subhumid bioclimatic zone. Specimens were collected from July to October and dissected to assess ovarian development and egg-laying activity. The number of ovarioles, determined at birth, ranged from 62 to 67 for C. wattenwylianus and 48 to 58 for C. barbarus. Higher fertility was observed in C. wattenwylianus, with egg production increasing during the first oviposition for both species. The average reproductive yield was notable for C. barbarus (85.99%) and C. wattenwylianus (86.56%). Oocyte resorption occurred mainly during drought (August-September) and cold periods (October-November). C. wattenwylianus females reached maturity earlier, beginning in August, compared to C. barbarus. Egg production began in July for C. wattenwylianus and in September for C. barbarus. Oocyte retention rates were 5% for C. barbarus and 9% for C. wattenwylianus. These findings provide insights into reproductive patterns in North African Orthoptera, with implications for pest control in subhumid regions.

Advanced Nuclear Magnetic Resonance, Fourier Transform–Infrared, Visible-NearInfrared and X-ray Diffraction Methods Used for Characterization of Organo-Mineral Fertilizers Based on Biosolids

Biosolids from stabilized sludge present a high fertilization potential, due to their rich content of nutrients and organic matter. The intrinsic and subtle properties of such fertilizers may greatly influence the fertilization efficiency. In this sense, the utility, advantages and limitations of advanced characterization methods, for the investigation of structural and dynamic properties at the microscopic scale of slightly different formulations of fertilizers were assessed. For that, three formulas of organo-mineral fertilizers based on biosolids (V1, V2 and V3), having at least 2% N, 2% P2O5, and 2% K2O, were characterized by advanced methods, such as 1H NMR relaxometry, 1H MAS and 13C CP-MAS NMR spectroscopy, 1H double-quantum NMR and FT-IR spectroscopy. Advanced structural characterization was performed using SEM, EDX and X-ray diffraction. Four dynamical components were identified in the NMR T2 distribution showing that the rigid component has a percentage larger than 90%, which explains the broad band of NMR spectra confirmed by the distributions of many components in residual dipolar coupling as were revealed by 1H DQ-NMR measurements. SEM and EDX measurements helped the identification of components from crystalline-like X-ray diffraction patterns. To evaluate the release properties of organo-mineral fertilizers, dynamic measurements of classical electric conductivity and pH were performed by placing 0.25 g of the formulas (V1, V2 and V3) in 200 mL of distilled water. The content of N and P were quantified using specific reactants, combined with VIS-nearIR spectroscopy. Two release mechanisms were observed and characterized. It was found that V3 presents the smallest release velocity but releases the largest number of fertilizers.

Effect of Crop Protection Intensity and Nitrogen Fertilisation on the Quality Parameters of Spelt Wheat Grain cv. ‘Rokosz’ Grown in South-Eastern Poland

Spelt wheat is one of the oldest wheats cultivated by humans. It is characterised by high nutritional values and is sought after by consumers. Additionally, it does not have high habitat and fertilisation requirements and is resistant to diseases. The aim of this study was to determine the effect of different levels of nitrogen fertilisation and the intensification of fungicide protection on the grain quality characteristics of spelt cv. ‘Rokosz’ grown under south-eastern Polish conditions. The present research showed that the intensification of fungicide crop protection and increasing the nitrogen dose from 70 to 130 kg ha−1 had a positive effect on the quality features of spelt grains. The highest protein, gluten and starch contents were found after four fungicide treatments. These parameters increased their values under the influence of fungicides. After the application of 130 kg ha−1, spelt wheat grain had the most favourable chemical composition, containing the most protein, gluten, soluble dietary fibre, insoluble dietary fibre and fat. It also had a positive effect on the Zeleny sedimentation index and the amino acid content of the grain. Due to the favourable response of the spelt cv. ‘Rokosz’ to intensified fungicide protection and nitrogen fertilisation, it should be recommended for cultivation in integrated technology.

Harnessing Nitrogen Use Efficiency for Enhancing Productivity in Rice: A Review

Nitrogen fertilizers have significantly enhanced rice yields over the past decades, but they also have substantial negative impacts on the environment. Excessive application of nutrients, particularly nitrogen, beyond crop requirements is linked to environmental losses. High nitrogen fertilizer input leads to low nitrogen use efficiency (NUE) due to the rapid loss of nitrogen through ammonia volatilization, denitrification, surface runoff, and leaching in the soil-flood water system. Enhancing the yield potential of rice- based cropping systems in response to the global call for carbon neutrality is a crucial research target. New approaches are needed to boost yields while maintaining or ideally reducing nitrogen application to maximize crop NUE. Improving NUE is crucial for addressing the intertwined challenges of environmental degradation, climate change, and food security. This review provides an in-depth exploration of strategies for improving nitrogen use efficiency, including soil health management, optimized fertilizer application, crop management practices, physiological and morphological approaches, exploiting symbiotic relationships, and genetic approaches, all aimed at enhancing rice productivity while addressing agricultural and environmental challenges caused by nitrogen losses, such as ammonia volatilization and leaching and promoting more sustainable farming practices. It also reduces the input costs, leading to a more sustainable agricultural economy and improved living standards for farming communities.

Nitrogen input strategies impact fertilizer nitrogen saving by intercropping: A global meta-analysis

ContextSeveral meta-analyses have demonstrated that intercropping increases land use efficiency and fertilizer use efficiency of grain-producing crop species, but no overarching synthesis has been made on the effects of fertilizer management on fertilizer use efficiency. Research questionsHere we assess the relative N use efficiency of species mixtures compared to sole crops under different N input strategies using global data. MethodsWe built a global database of yield and fertilizer N input with 600 data records representing the results of 136 independent experiments from 80 publications with four main types of species functional combinations (SFCs), based on key traits that were found relevant in previous intercropping studies: C3-cereal/legume, maize/legume, C4-non-maize/legume and maize/C3-cereal. ResultsWe found that the literature reports results for four main N input strategies in the intercrop and sole crops: (1) zero fertilizer N input (Nic=N1=N2=0), (2) equal fertilizer N input (Nic=N1=N2>0), (3) intermediate fertilizer N input (N1≥Nic≥N2 and N1>N2), and (4) transgressive fertilizer N input (Nic>N1≥N2), where N1, N2 and Nic represent the N input in sole crop 1, sole crop 2 and the intercrop. With zero N input, high land equivalent ratio was found in cereal/legume intercrops but not in maize/C3-cereal intercrops. Intermediate N input (strategy 3) resulted in high LER (land equivalent ratio) and FNER (fertilizer N equivalent ratio) because of the high intercropping advantage of cereals in C3-cereal/legume, maize/legume, and C4-non-maize/legume intercrops and maize in maize/C3-cereal intercrops. Equal N input in the sole crops and the intercrops resulted in LER and FNER being equal and N saving was entirely due to land saving, regardless of SFCs. Transgressive N input resulted in high LER but low FNER. ConclusionsThe study confirms that cereal/legume intercropping increases LER at zero N input. Strategies that tailor N input in intercropping to species demand (strategy 3) pair high productivity and LER to high fertilizer N use efficiency. The transgressive N input strategy maximizes LER at the expense of FNER, thus potentially generating high N losses.

On-farm experimentation of precision agriculture for differential seed and fertilizer management in semi-arid rainfed zones

Abstract Introduction This study explores the integration of precision agriculture technologies (PATs) in rainfed cereal production within semi-arid regions. Methods utilizing the Veris 3100 sensor for apparent soil electrical conductivity (ECa) mapping, differentiated management zones (MZs) were established in experimental plots in Valsalada, NE Spain. Site-specific variable dose technology was applied for seed and fertilizer applications, tailoring inputs to distinct fertility levels within each MZ. Emphasizing nitrogen (N) management, the study evaluated the impact of variable-rate applications on crop growth, yield, nitrogen use efficiency (NUE), and economic returns. For the 2021/2022 and 2022/2023 seasons, seeding rates ranged from 350 to 450 grains/m2, and basal fertilizer dosages varied between high and low levels. Additionally, the total nitrogen units were distributed differently between the two seasons, while maintaining a uniform topdressing fertilizer dose across all treatments. Results Results revealed a significant increase in yield in MZ 2 (higher fertility) compared to MZ 1 (lower fertility). NUE demonstrated notable improvement in MZ 2, emphasizing the effectiveness of variable-rate N applications. Economic returns, calculated as partial net income, showed a considerable advantage in MZ 2 over MZ 1, resulting in negative outcomes for low-fertility areas in several of the analyzed scenarios, and highlighting the financial benefits of tailored input management. Conclusion This research provides quantitative evidence supporting the viability and advantages of adopting PATs in rainfed cereal production. The study contributes valuable insights into optimizing input strategies, enhancing N management, and improving economic returns in semi-arid regions.

Higher incomes are increasingly associated with higher fertility: Evidence from the Netherlands, 2008–2022

Higher incomes are increasingly associated with higher fertility: Evidence from the Netherlands, 2008–2022

Financial Feasibility of Cucumber Cultivation in Polyhouse: A Case Study from Jaipur, Rajasthan, India

Cucumber (Cucumis sativus L.) is one of the popular vegetable crops grown broadly throughout the world. Cucumber crop grows successfully under conditions of high light, high humidity, high soil moisture, temperature, and fertilizers in the greenhouse. Aim of the Study: The present study was carried out to evaluate financial matrix such as payback period or net present value benefit cost ratio and internal rate of return of polyhouse by cucumber cultivation financial metrics evaluated, for 2023-24. Sampling Design: A multistage sampling design were used for the study of Cucumber cultivation in a polyhouse in the Jaipur district of Rajasthan for the 2023-24. Two tehsils were selected purposively based on the highest number of polyhouse from Jaipur district, namely Jhotwara and Jobner. A sample of 60 farmers were selected for the study from these two tehsils. Results: The finding of the study revealed that the payback period of polyhouse by cucumber cultivation, for small farmers was favorable after covering the subsidy i.e. 0.75 years. The net present worth of polyhouse for small farmers was ₹ 14222249.12, for 20-year expected life at a 7.5 percent discount rate. It was ₹ 13406743.12 for large farmers and ₹ 11164102.12 for assumed non-subsidized farmers. The benefit-cost ratio for the subsidized small farmer was 10.41 and it was 6.76 for the subsidized large farmer. The benefit-cost ratio for the assumed non-subsidized farmer was 3.44 for the cucumber cultivation in polyhouse. The Internal Rate of Return was 133 percent, 84 percent, and 36 percent for the small, large, and non-subsidized cucumber-cultivating polyhouse farmers. Thus, result of the study in all three condition shows profitability of polyhouse by cucumber cultivation.

Greenhouse gas emissions during alfalfa cultivation: How do soil management and crop fertilisation of preceding maize impact emissions?

ContextThe use of alfalfa in rotation with intensive crops is common practice to mitigate the physical and chemical issues arising from intensive farming practices. However, there is a dearth of studies on this practice. Given the current concern regarding climate change and the significant impact agriculture has on greenhouse gas (GHG) emissions, understanding the emissions associated with this practice, as well as the most suitable soil and crop management techniques for their mitigation, is of paramount importance. ObjectiveThe present study aimed to (i) quantify emissions of N2O, CO2 and CH4 in an alfalfa crop following a maize cropping scenario; (ii) to determine which tillage system generates the lowest GHG emissions, and; (iii) to determine how N fertilisation from a preceding intensive maize crop affects GHG emissions during alfalfa cropping period. MethodsA three-year field experiment (2019, 2020 and 2021) was conducted to assess the emissions of N2O, CO2 and CH4 from alfalfa cultivation following a three-year period of irrigated maize. Two soil management practices (no-tillage and conventional tillage) were implemented during both the maize cropping period and the alfalfa establishment. Additionally, the nitrogen (N) fertilisation rates applied to the preceding maize crop were included as a treatment (0, 200, and 400 kg N ha⁻¹, corresponding to zero, medium, and high fertilisation levels, respectively) in a randomized block design with two factors. ResultsEmissions of N2O in alfalfa ranged from 0.05 to 0.32 mg N2O-N m⁻² day⁻¹, being significantly higher only during first month of sampling in the treatments that had received fertilisation. CO2 emissions ranged from 1158 to 4258 mg CO2-C m⁻² day⁻¹. Year-average CH4 fluxes were −0.27 g C ha⁻¹ day⁻¹. The average total dry matter produced by alfalfa was 17700 kg ha⁻¹ year⁻¹, being higher for the no-tillage treatment, though significantly so only during first month of sampling. ConclusionsUnder Mediterranean conditions, the tillage system and mineral N fertilizer rates have a relative effect on greenhouse gas emissions during the alfalfa cropping period. Plots without N fertilization initially produced lower N2O emissions and higher total dry matter, resulting in the lowest scaled emissions. For the tillage treatment, no significant differences were found in emission dynamics, which may be due to the fact that alfalfa does not involve soil disturbance, leading to a homogenization of the treatments. However, the NT treatment showed lower scaled emissions due to higher yields in the first year. Therefore, alfalfa cultivation is characterized by low GHG emissions, high yields, and a notable capacity to mitigate the negative effects of previous intensive crops. SignificanceThis study provides data on GHG emissions during alfalfa cropping in the typical Maize-alfalfa crop rotation under Mediterranean irrigated systems, which is useful for new agricultural policies aimed at reducing pollution in the agricultural sector. Additionally, it demonstrates the capability of this crop to mitigate adverse agronomic and environmental conditions caused by preceding intensive farming practices.

Barriers to modern contraceptive utilization in Ethiopia.

Contraception is a critical service for women to control their reproductive health, allowing them to determine the number and spacing of their children prevent unintended pregnancies, reduce the risk of morbidity and mortality from associated with childbirth, and reduce the likelihood of abortions. Despite its benefits, the utilization of modern contraceptive methods remains low in certain regions of Ethiopia, Particularly in Afar and Somali, which are also experiencing high fertility rates. However, there is a substantial gap in understanding the sociocultural barriers that hinder the adoption of modern contraception in these regions. This study aims to explore these barriers to contraceptive use among women of reproductive age in Afar and Somali regions, providing qualitative insights that are essential for designing effective strategies to improve contraceptive service utilization. Asequential mixed method approach was employed, including scoping review and qualitative interviews. Scoping review focused on qualitative or mixed-methods studies conducted in Ethiopia and published between 2013 and 2023 in English, Selecting 14 articles Additionally, qualitative data were collected from Afar and Somali regions through In-depth interviews with women aged 15-49 who are married or in consensual union, and key informant interviews with health extension workers and contraception providers at health center. A total of 20 in-depth interviews and 07 key informants were conducted. Thematic analysis was used to analyze the data. The integrated findings from the scoping review and qualitative study reveal various barriers and determinants influencing contraceptive use among women in Ethiopia. Barriers includes religious or cultural beliefs, myths and misconceptions, fear of side effects, lack of knowledge and misinformation, negative attitude towards contraceptives, partner opposition, socio-cultural factors, fear of being judged by family and friends, and lack of communication between husband and wife, husband altitude, distance from health facility, availability of service and different contraceptive choice, separate room for family planning services, and cost of contraceptive method and transportation. The scoping review corroborates these findings, emphasizing on the role of socio-demographic, economic, cultural, religious, health service, and knowledge-related factors. Higher education, urban residence, higher income, mass media exposure, spousal communication, family size, and access to quality health services were associated with increased utilization, while lack of awareness, misconceptions, myths, side effects, fear of infertility, partner opposition, social stigma, and cultural norms decreased utilization. The study recommended promoting contraceptive use, challenging socio-cultural norms through Social Behavioural Communication and Counselling (SBCC), engaging partners and community members, and improving the quality of care.

Identification of structural variation related to spawn capability of Penaeus vannamei

BackgroundThe genetic basis underlying spawning abilities in the Pacific white shrimp, Penaeus vannamei, remains largely unexplored. To investigate genetic variations potentially related to reproductive performance, a systematic bioinformatic analysis was conducted to identify structural variations (SVs) with different polymorphic spectra in P. vannamei with high fertility (HF) and low fertility (LF).ResultsA total of 2,323 and 1,859 SV events were identified exclusively in the HF and LF groups, respectively. These SVs were mapped to 277 genes in the HF group and 231 genes in the LF group. Gene Ontology (GO) enrichment analysis based on SNPs (single nucleotide polymorphism) and SVs revealed several neural-related processes, suggesting the importance of neural regulation in reproduction. Notably, we identified a set of promising genes, including Cttn, Spast, Ppp4c, Spire1, Lhcgr, and Ftz-f1, which may enhance fertility in shrimp.ConclusionIn conclusion, this study is the first to establish a link between SVs and reproductive traits in P. vannamei. The promising genes discovered have the potential to serve as crucial markers for enhancing reproductive traits through targeted genotyping.