High Crowd Research Articles

Protein-binding dynamics imaged in a living cell

Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated by microinjection and monitored by Förster resonance energy transfer. Association rate constants for the wild-type and an electrostatically optimized mutant were only 25% and 50% lower than in vitro values, whereas no change in the rate constant was observed for a slower binding mutant. These changes are much smaller than might be anticipated considering the high macromolecular crowding within the cell. Single-cell analyses of association rate constants and fluorescence recovery after photobleaching reveals a naturally occurring variation in cell density, which is translated to an up to a twofold effect on binding rate constants. The data show that for this model protein interaction the intracellular environment had only a small effect on the association kinetics, justifying the extrapolation of in vitro data to processes in the cell.

Changes in the crown angulation and dental arch widths after nonextraction orthodontic treatment: Model analysis of mild crowding with high canines

This study was undertaken to examine which factors contributed to the correction of crowding in two patients who underwent nonextraction orthodontic treatment. A study model analysis was conducted to determine the effects of the orthodontic treatment for crowding with high canines on crown angulation and dental arch width in two patients. The results showed that the crown angulation was significantly increased, indicating distal tipping in the maxillary dental arch. This tendency was most commonly observed in the premolars among the lateral teeth. With respect to the dental arch width, the largest change was evident in the first molar and first premolar regions in cases 1 and 2, respectively. On the basis of these results, up-righting of mesially tipped lateral teeth and expansion of narrow dental arches could prove to be the keys to the success of space regaining or correction of high canines and mild crowding.

Multidimensional trade‐offs in species responses to disturbance: implications for diversity in a subtropical forest

Species employ diverse strategies to cope with natural disturbance, but the importance of these strategies for maintaining tree species diversity in forests has been debated. Mechanisms that have the potential to promote tree species coexistence in the context of repeated disturbance include life history trade-offs in colonization and competitive ability or in species' ability to survive at low resource conditions and exploit the temporary resource-rich conditions often generated in the wake of disturbance (successional niche). Quantifying these trade-offs requires long-term forest monitoring and modeling. We developed a hierarchical Bayes model to investigate the strategies tree species employ to withstand and recover from hurricane disturbance and the life history trade-offs that may facilitate species coexistence in forests subject to repeated hurricane disturbance. Unlike previous approaches, our model accommodates temporal variation in process error and observations from multiple sources. We parameterized the model using growth and mortality data from four censuses of a 16-ha plot taken every five years (1990-2005), together with damage data collected after two hurricanes and annual seed production data (1992-2005). Species' susceptibilities to hurricane damage as reflected by changes in diameter growth and fecundity immediately following a storm were weak, highly variable, and unpredictable using traditional life history groupings. The lower crowding conditions (e.g., high light) generated in the wake of storms, however, led to greater gains in growth and fecundity for pioneer and secondary-forest species than for shade-tolerant species, in accordance with expectation of life history. We found moderate trade-offs between survival in high crowding conditions, a metric of competitive ability, and long-distance colonization. We also uncovered a strong trade-off between mean species fecundity in low crowding conditions, a metric of recovery potential, and competitive ability. Trade-offs in competitive and colonization ability, in addition to successional niche processes, are likely to contribute to species persistence in these hurricane-impacted forests. The strategies species employ to cope with hurricane damage depend on the degree to which species rely on sprouting, repair of adult damage, changes in demographic rates in response to enhanced resource availability after storms, or long-distance dispersal as recovery mechanisms.

CagA Helicobacter pylori Seropositivity in Asymptomatic, Apparently Healthy, Young Adult Egyptian Food Handlers

Background/Aim: Infection with CagA Helicobacter pylori(H. pylori) positive strain is a risk factor for the development ofatrophic gastritis and gastric adenocarcinoma. This studyinvestigated the seroprevalence of H. pylori and its virulentstrains CagA in Egyptian food handlers.Subjects and methods: A total of 365 eligible male subjectsparticipated in the initial study. Venous blood samples werecollected from all participants for assessment of H. pylori specificIgG and serum CagA antibodies.Results: Out of 365 subjects, 310 were reactive to H. pylori.Out of them, CagA was detected in 143 H. pylori-infectedsubjects. CagA antibodies were more common in cigarettesmokers (< 0.001), living in high crowding index (< 0.001), withlow family income (< 0.01) and sharing their bedrooms duringchildhood (< 0.001).Conclusion: Our data revealed a high prevalence of CagAvirulent strains among asymptomatic apparent food handlersof Egypt. Emergency surveillance mechanisms should bedeveloped for containment of H. pylori infection in Egypt.Abbreviations: H. pylori: Helicobacter pylori; CagA: Cytotoxinassociated gene A.

Smoothed Particle Hydrodynamics 기반 고 밀집 군중 시뮬레이션 기법

고 밀집 상태의 군중 시뮬레이션은 객체 수에 따라 복잡도와 제작 비용이 크게 증가함으로 사실적인 움직임을 표현하는데 어려움이 있다. 본 논문에서는 고 밀집 군중 시뮬레이션 시 사실적인 움직임을 표현하기 위해 Smoothed Particle Hydrodynamics (SPH) 기법을 도입하였다. 본 연구에서는 유체 시뮬레이션에 사용되는 SPH 모델을 응용하여 객체 움직임에 필요한 회피력, 거리 유지력, 그룹 응집력을 새로이 제안하였다. 제안된 객체 운동 수식은 고 밀집 상태에서 유체와 같이 자연스런 객체 움직임을 표현하는데 효과적이다. 실험 결과, 본 시스템은 밀집도 높은 군중 시뮬레이션을 실시간으로 생성 가능함을 보였다.

International Perspectives on Emergency Department Crowding

The maturation of emergency medicine (EM) as a specialty has coincided with dramatic increases in emergency department (ED) visit rates, both in the United States and around the world. ED crowding has become a public health problem where periodic supply and demand mismatches in ED and hospital resources cause long waiting times and delays in critical treatments. ED crowding has been associated with several negative clinical outcomes, including higher complication rates and mortality. This article describes emergency care systems and the extent of crowding across 15 countries outside of the United States: Australia, Canada, Denmark, Finland, France, Germany, Hong Kong, India, Iran, Italy, The Netherlands, Saudi Arabia, Catalonia (Spain), Sweden, and the United Kingdom. The authors are local emergency care leaders with knowledge of emergency care in their particular countries. Where available, data are provided about visit patterns in each country; however, for many of these countries, no national data are available on ED visits rates or crowding. For most of the countries included, there is both objective evidence of increases in ED visit rates and ED crowding and also subjective assessments of trends toward higher crowding in the ED. ED crowding appears to be worsening in many countries despite the presence of universal health coverage. Scandinavian countries with robust systems to manage acute care outside the ED do not report crowding is a major problem. The main cause for crowding identified by many authors is the boarding of admitted patients, similar to the United States. Many hospitals in these countries have implemented operational interventions to mitigate crowding in the ED, and some countries have imposed strict limits on ED length of stay (LOS), while others have no clear plan to mitigate crowding. An understanding of the causes and potential solutions implemented in these countries can provide a lens into how to mitigate ED crowding in the United States through health policy interventions and hospital operational changes.

Associations between sleep-disordered breathing symptoms and facial and dental morphometry, assessed with screening examinations

Chronic snoring is considered abnormal in a pediatric population. This disorder is often attributed to enlarged tonsils and adenoids, but multiple anatomic obstructions should also be considered. Facial and dental morphometry associations with various sleep-disordered breathing symptoms were investigated at an orthodontic clinic. Parents or guardians were asked to complete a 4-part questionnaire on behalf of their children (n = 604; <18 years of age), including medical and dental history, bruxism and temporomandibular disorder habits, sleep and daytime behavior, and sleep duration and quality. All subjects underwent a clinical screening assessment by the same orthodontist to identify standard dental, skeletal, functional, and esthetic factors. In contrast to sleep-disordered breathing or sleep apnea in adults, which is predominantly associated with obesity, sleep-disordered breathing symptoms in this pediatric cohort were primarily associated with adenotonsillar hypertrophy, morphologic features related to a long and narrow face (dolichofacial, high mandibular plane angle, narrow palate, and severe crowding in the maxilla and the mandible), allergies, frequent colds, and habitual mouth breathing. Because of the recognized impact of pediatric snoring on children's health, the determination of these good predictors can help in preventing and managing sleep-disordered breathing. If a health professional notices signs and symptoms of sleep-disordered breathing, the young patient should be referred to a sleep medicine specialist in conjunction with an orthodontist if there are dentoskeletal abnormalities.

Visitor Effects on the Behavior of Captive Western Lowland Gorillas: The Importance of Individual Differences in Examining Welfare

Given the conflicting and somewhat limited findings available on the effect of zoo visitors on primate behavior, the primary purpose of this study was to provide additional data on gorillas' response to variations in crowd size and to look at what other factors-both intrinsic (e.g. personality, sex, and rearing history) and extrinsic (e.g. group)-might influence such responses. Subjects included four groups (three mixed-sex and one bachelor) of captive western lowland gorillas living at Zoo Atlanta. Overall activity budgets and rates of social behaviors in high and low crowd conditions were compared. Behaviors suggestive of general welfare did not vary as a function of crowd size alone, although we did observe one significant interaction and several trends. Specifically, two groups spent more time engaged in undesirable behaviors in the high crowd condition, whereas the other two groups showed the opposite response pattern. Additionally, males, but not females, showed a trend toward greater rates of contact aggression in the high crowd condition, although wounding rates did not vary with crowd size. We also found trends towards variation in response to crowd size as a function of individual personality ratings. These results suggest that although the visitor effect may constitute an impediment to optimal animal welfare, this may only apply to some individuals or groups. We emphasize the importance of continuing to explore individual differences and the limitations of a one-size-fits-all approach when describing influences on animal welfare.

CDES: A pixel-based crowd density estimation system for Masjid al-Haram

CDES is an automatic crowd density estimation system that can be used to estimate crowd density from digital images taken at Masjid al-Haram. Developed using a combination of image processing and artificial intelligence (AI) technologies, CDES possesses the capability to count the number of people in moderately high crowds from a flexibly selected region of interest (ROI). Background removal and edge detection are first applied to the image for crowd feature extraction. Then, the extracted crowd foreground blob pixels are scaled accordingly to correct perspective distortion. Finally, the corrected pixel blobs act as input for the backpropagation (BP) neural network to estimate the number of people within the blob. Using the area of the selected ROI, the crowd density is calculated and classified into five ranges from very low to very high. The experimental results are presented.

Sparsity Driven People Localization with a Heterogeneous Network of Cameras

This paper addresses the problem of localizing people in low and high density crowds with a network of heterogeneous cameras. The problem is recast as a linear inverse problem. It relies on deducing the discretized occupancy vector of people on the ground, from the noisy binary silhouettes observed as foreground pixels in each camera. This inverse problem is regularized by imposing a sparse occupancy vector, i.e., made of few non-zero elements, while a particular dictionary of silhouettes linearly maps these non-empty grid locations to the multiple silhouettes viewed by the cameras network. The proposed framework is (i) generic to any scene of people, i.e., people are located in low and high density crowds, (ii) scalable to any number of cameras and already working with a single camera, (iii) unconstrained by the scene surface to be monitored, and (iv) versatile with respect to the camera's geometry, e.g., planar or omnidirectional. Qualitative and quantitative results are presented on the APIDIS and the PETS 2009 Benchmark datasets. The proposed algorithm successfully detects people occluding each other given severely degraded extracted features, while outperforming state-of-the-art people localization techniques.

On the performance of bioanalytical fluorescence correlation spectroscopy measurements in a multiparameter photon-counting microscope

Fluorescence correlation spectroscopy (FCS) data acquisition and analysis routines were developed and implemented in a home-built, multiparameter photon-counting microscope. Laser excitation conditions were investigated for two representative fluorescent probes, Rhodamine110 and enhanced green fluorescent protein (EGFP). Reliable local concentrations and diffusion constants were obtained by fitting measured FCS curves, provided that the excitation intensity did not exceed 20% of the saturation level for each fluorophore. Accurate results were obtained from FCS measurements for sample concentrations varying from pM to μM range, as well as for conditions of high background signals. These experimental constraints were found to be determined by characteristics of the detection system and by the saturation behavior of the fluorescent probes. These factors actually limit the average number of photons that can be collected from a single fluorophore passing through the detection volume. The versatility of our setup and the data analysis capabilities were tested by measuring the mobility of EGFP in the nucleus of Drosophila cells under conditions of high concentration and molecular crowding. As a bioanalytical application, we studied by FCS the binding affinity of a novel peptide-based drug to the cancer-regulating STAT3 protein and corroborated the results with fluorescence polarization analysis derived from the same photon data.

Thresholds in the Finance-Growth Nexus: A Cross-Country Analysis

Thresholds of inflation, government size, trade openness, and per capita income for the finance-growth nexus are investigated using five-year averages of standard variables for 84 countries from 1965 to 2004. The results suggest that: (i) high inflation crowds out positive effects of financial depth on long-run growth, (ii) small government sizes hurt the finance-growth nexus in low-income countries, while large government sizes hurt high-income countries, (iii) low levels of trade openness are sufficient for finance-growth nexus in high-income countries, but low-income countries need higher levels of trade openness for similar magnitudes of the finance-growth nexus, and (iv) catch-up effects through the finance-growth nexus are higher for moderate per capita income levels.

Evaluation of ligand effects in the modified cobalt hydroformylation of 1-octene. Crystal structures of [Co(L)(CO)3]2 (L = PA–C5, PCy3 and PCyp3)

A series of phosphine ligands with different electronic and steric properties were evaluated at fully modified conditions in cobalt catalysed hydroformylation of 1-octene. The steric demand of the ligands was based on the Tolman cone angle model covering a range of 132-175°. The electron donating ability was evaluated through the first order Se-P coupling constants as determined from the corresponding phosphine selenides covering a range of 672-752 Hz. Crystal structures of three phosphine modified cobalt dimers, [Co(CO)(3)(L)](2) (L = PA-C(5), PCy(3) and PCyp(3) with PA-C(5) = 1,3,5,7-tetramethyl-8-pentyl-2,4,6-trioxa-8-phosphatricyclo[3.3.1.1(3,7)]decane), are reported. The Phoban and Lim ligands (Phoban = mixture of 9-phosphabicyclo[3.3.1 and 4.2.1]nonane, Lim = 4,8-dimethyl-2-phosphabicyclo[3.3.1]nonane) resulted in systems about twice as active as most of the other ligands investigated, these ligands have a high Lewis basicity with (1)J(Se-P) values from 684-687 Hz. The linearity of the alcohol product in general decreased for the less electron donating ligands while no clear relationship was evident as a function of steric size. The parallel competing hydrogenation of 1-octene to octane varied from 9-15% for a cone angle range of 132-172°, but a sharp increase of up to 40% was observed for PA-C(5), PCy(3) and PCyp(3), all with cone angles > 169°. The catalytic behaviour provides evidence that is contrary to the dissociative substitution of CO by an alkene as the rate limiting step in all cases. For large symmetrical ligands, such as PA-C(5), PCy(3) and PCyp(3) the rate limiting step may move within the catalytic cycle and may now be situated at the carbonylation step where the chemoselectivity is also determined. The lack of clear correlation between the steric and electronic effect of the ligands and all catalytic parameters may serve as additional proof that the same system, especially in terms of the rate determining step, is not operative in all cases. The Phoban and Lim systems are superior with the highest reactivity and lowest alkene loss through hydrogenation. The unsymmetrical nature of the Phoban and Lim ligands may provide flexibility to adopt geometries inducing both high and low steric crowding, which may be a reason for its beneficial catalytic properties.

Asymmetric Coordination Chemistry by Chiral‐Auxiliary‐Mediated Dynamic Resolution under Thermodynamic Control

A method is presented for the asymmetric synthesis of chiral ruthenium polypyridyl complexes that starts from racemic cis-[Ru(pp)(2)Cl(2)] (pp=2,2'-bipyridine or 1,10-phenanthroline ligands). The chiral bidentate ligands (R)-2-(isopropylsulfinyl)phenol, (R)-SO, and preferably the more electron-rich derivative (R)-2-(isopropylsulfinyl)-4-methoxyphenol, (R)-SO', serve as convenient chiral auxiliaries for the conversion of racemic starting complexes (1a: pp=2,2'-bipyridine; 1b: pp=5,5'-dimethyl-2,2'-bipyridine; c: pp=1,10-phenanthroline) into single diastereomers Λ-[Ru(pp)(2){(R)-SO}]PF(6) (Λ-(S)-2a-c) or Λ-[Ru(pp)(2){(R)-SO'}]PF(6) (Λ-(S)-2a') under a thermodynamically controlled dynamic transformation. The complexes Λ-(S)-2a-c and Λ-(S)-2a' themselves are direct precursors for the generation of optically active ruthenium-polypyridyl complexes by trifluoroacetic-acid-induced replacement of the sulfinylphenolate auxiliaries with bidentate pp ligands under retention of configuration, thereby affording Λ-[Ru(pp)(3)](PF(6))(2) (3a-c) complexes with high enantiomeric ratios of ≥98:2. In particular, by employing the methoxy-modified chiral auxiliary (R)-SO', enantiomeric ratios of >99:1 were reached. In the strategy introduced here, the high steric crowding of an octahedral coordination sphere was exploited by placing a sulfur-based stereocenter in direct proximity to the ruthenium stereocenter, thereby leading to a large difference in the stabilities of the intermediate Λ-S and Δ-S diastereomers and thus providing the opportunity to find suitable reaction conditions for conversion of the destabilized diastereomer into the thermodynamically more-stable one. This method should be of high practical value for the asymmetric synthesis of ruthenium-polypyridyl complexes because it allows one to use readily available racemic ruthenium complexes as starting materials.

Efficient Cable Shovel Excavation in Surface Mines

The cable shovel is widely used in surface mining. High operating and ownership costs necessitate efficient use of the cable shovel. Operator practices have long been suspected to contribute towards the inefficient use of the shovel. Crowd arm and hoist rope speeds are key measures of operator practices. The objective of this work is to find the crowd arm and hoist rope speeds for optimal shovel performance for given initial conditions and material properties. Shovel kinematics and dynamic modeling, using shovel geometry and the simultaneous constraint method, respectively, have been employed to build models of the excavation process. Dynamic models of the shovel payload and the material cutting resistance have also been developed using geometric simulation and passive soil pressures techniques, respectively. These models are solved numerically by combining Runge–Kutta and Gaussian elimination algorithms to compute the work done and the resistive forces during shovel excavation. The algorithms have been combined into a shovel simulator. The simulator has been used to simulate the P&H 2100BL shovel. The simulation results indicate that input energy and digging time increase with increasing crowd arm and decreasing hoist rope speeds. The input energy per unit loading rate is proposed as an appropriate measure of shovel performance. High energy per unit loading rate occurs for high crowd speeds and low hoist rope speeds. For the simulated conditions and crowd arm and hoist rope speeds ranging from 0.25 to 0.5 ms−1 and 0.5 to 0.7 ms−1, respectively, the optimal crowd arm and hoist rope speeds were found to be 0.25 ms−1 and 0.7 ms−1, respectively, and the objective function value was 0.21 KJs/kg. This work establishes, theoretically, the fact that operator practices have an effect on shovel performance and is useful in establishing optimum practices. The results are the initial steps towards full automation of the excavation process.

Is melanism in pygmy grasshoppers induced by crowding?

Color polymorphisms in animals may result from plasticity of the developmental system in response to genetic cues in the form of allelic variation at polymorphic loci, environmental cues, or a combination of genetic and environmental cues. An increased understanding of the evolution of color polymorphisms requires better knowledge of when we should expect genetic and environmental cues respectively to influence phenotype determination. Theory posits that the developmental systems of organisms should evolve sensitivity to such cues that most accurately predict coming selective conditions. Pygmy grasshoppers (Orthoptera, Tetrigidae) vary in color pattern within and among populations and show fire melanism, i.e., an increased frequency of black and dark colored phenotypes in high density populations inhabiting fire-ravaged areas. We examined if the population density experienced by individuals during development influenced the phenotypic expression of color pattern in Tetrix subulata. Individuals were experimentally reared either in solitude, at intermediate density or under crowded conditions. We found that color patterns of experimental individuals were independent of rearing density but strongly influenced by maternal color pattern. High population density and crowding may not constitute reliable predictors of the selective regime that characterizes post-fire environments.

The role of crowd theory in determining the use of force in public order policing

Social psychological research suggests that where police hold a theoretical view of the crowd in line with the ‘classic’ crowd psychology of Gustavé Le Bon this can lead to police practices that inadvertently escalate public disorder. This research reflects debates within the criminology literature which suggests that a primary factor governing police tactics is police knowledge. However, the existing research on the specific relationships between police theoretical knowledge of crowds and their practice towards them is limited by its reliance on post hoc data. This paper addresses this limitation by examining the role of police theoretical view of crowd psychology and their operational practice during a high risk football crowd event. The analysis supports the argument that when the police hold a view of the crowd as inherently irrational and dangerous they rely upon tactics of mass containment and dispersal. This study advances the literature by suggesting that this ‘classic’ theoretical view of the crowd is leading to missed opportunities for the police to develop more efficient, effective and less confrontational approaches to the management of public order during crowd events.

Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988-2004.

BACKGROUND. Congenital cytomegalovirus (CMV) infection causes permanent disabilities in more than 5500 children each year in the United States. The likelihood of congenital infection and disability is highest for infants whose mothers were CMV seronegative before conception and who acquire infection during pregnancy. METHODS. To provide a current, nationally representative estimate of the seroprevalence of CMV in the United States and to investigate trends in CMV infection, serum samples from the National Health and Nutrition Examination Survey (NHANES) 1999-2004 were tested for CMV-specific immunoglobulin G antibody, and results were compared with those from NHANES III (1988-1994). Individuals aged 6-49 years (21,639 for NHANES III and 15,310 for NHANES 1999-2004) were included. RESULTS. For NHANES 1999-2004, the overall age-adjusted CMV seroprevalence was 50.4%. CMV seroprevalence was higher among non-Hispanic black and Mexican American children compared with non-Hispanic white children and increased more quickly in subsequent age groups. CMV seropositivity was independently associated with older age, female sex, foreign birthplace, low household income, high household crowding, and low household education. Compared with NHANES 1988-1994, the overall age-adjusted CMV seroprevalence for NHANES 1999-2004 was not significantly different. CONCLUSIONS. Many women of reproductive age in the United States are still at risk of primary CMV infection during pregnancy. There is an urgent need for vaccine development and other interventions to prevent and treat congenital CMV. The substantial disparities in CMV risk among seronegative women suggest that prevention strategies should include an emphasis on reaching racial or ethnic minorities and women of low socioeconomic status.

The differential effects of retail density: An investigation of goods versus service settings

Two studies investigate the impact of retail density and time pressure on shoppers' store attitudes and behavioral intentions. In a goods setting, experiment 1 results show a curvilinear pattern as the level of retail density increases. That is, individuals prefer a medium level of crowding to a store with low or high crowding. This inverted-U shaped crowding effect has not previously been tested in the retailing literature. Experiment 2 employs a service setting, where the relationship between retail crowding and outcome variables is found to be linear rather than curvilinear, except under conditions of time pressure. In contrast to the goods setting, individuals have more favorable attitudes and expect to pay more for a service as the level of crowding increases. Thus, our study findings suggest that the optimal level of crowding depends on the type of retail setting.

Special series: Molecular chaperones in protein folding and disease

Special series: Molecular chaperones in protein folding and disease