High Content Research Articles

Temporary immersion bioreactor as an efficient method for in vitro propagation of Agave marmorata

Agave marmorata is an important wild species in Mexico for mezcal and pulque production. Propagation by suckers and seeds is not sufficient to restore wild populations. The aim of this study was to develop a protocol for large-scale micropropagation of A. marmorata using a temporary immersion bioreactor (TIB). The effect of liquid medium using a TIB and semi-solid medium as the control treatment was compared. Different immersion frequencies and culture medium volumes per explant were evaluated in the TIB. In all treatments, the percentage of hyperhydricity, number of shoots per explant, shoot length, and total chlorophyll and carotenoid content were evaluated. In addition, the survival rate during acclimatization was evaluated. The results obtained show that the TIB with an immersion of 2 min every 8 h and with a culture medium volume of 50 mL had the best developmental parameters, with 19.60 shoots per explant, a shoot length of 1.74 cm and a percentage of hyperhydricity of 3.33 %. The highest chlorophyll content was observed in temporary immersion every 8 h, with 100, 75 and 50 mL of culture medium per explant. Regarding carotenoid content, the highest content was found at the 8 and 12 h immersion frequencies, with the culture medium volumes of 100, 75 and 50 mL per explant. For acclimatization, no differences were found in the survival rate between the culture systems evaluated. In conclusion, this study demonstrates that the use of a TIB is an alternative for large-scale micropropagation of A. marmorata.

Effect of combined additions of Sc, Zr and Ti on hot-cracking resistance and precipitation behaviour in Al-Mg alloy by L-PBF

The request for high-performance aluminum components prompts research into innovative alloys compatible with laser-based additive manufacturing processes, leveraging grain refiners in their composition to mitigate hot-cracking and enhance strength. While the addition of Sc and Zr in Al-Mg alloys has been widely investigated, the high cost and supply risks associated with Sc necessitate reducing its amount and replacing, at least partially, with other inoculants. In this preliminary study, the amount of Sc replaceable with different concentrations of Zr or Ti is evaluated investigating the laser powder bed fusion processability of three powder feedstocks: a pre-alloyed Al-Mg-Zr-Sc powder, a blend of the previous alloy with addition of Zr particles, and a further blend of an alloy depleted of Zr with added Ti particles. The experimental analyses on the laser-processed alloys showed that the standard and the Zr-enriched alloys featured a bimodal microstructure free from cracks with fine equiaxed grains at the edge of the molten pools and coarser grains at their centers. In the alloy variant depleted in Zr with addition of Ti particles a columnar structure was observed and hot-cracks appeared. Thermodynamic simulations of phase formation allowed defining the precipitation kinetics during solidification and direct aging, showing increased precipitation in alloys with higher Zr content, while the presence of Ti resulted in sluggish precipitation. Experimental aging tests demonstrated significant increases in microhardness, with peak values of the modified alloys achieved after 12 h at 375°C.

Metabolome and transcriptome reveal high abundance of bioactive substances in albino jujube fruit as potential function food

Little is known about the fruit quality of albino mutants, due to generally exhibited lethal phenotypes caused by restrained photosynthesis and energy metabolism. In this study, a non-lethal bud mutant of jujube that could bear fruit with higher nutrient content after leaf albinism was described. Compared to normal mature fruit, the albino fruit contained lower soluble sugar and titratable acid, as well as higher ascorbic acid, phenols and flavonoids contents. Through metabolome and transcriptome analyses, 109, 117, and 126 differentially accumulated metabolites (DAMs) and 1169, 797, and 3059 differentially expressed genes (DEGs) were identified in the young, white-ripened, and mature stages of albino and normal fruits, respectively. KEGG annotation indicated that DEGs and DAMs were mainly enriched in phenylpropanoid and flavonoid biosynthesis, glutathione metabolism, and α-linolenic acid metabolism. Moreover, ADH, GST17, and POD16 were identified as hub genes that play major roles in high abundance of chalcone, (−)-epigallocatechin, GSH, and total ascorbate. The alteration of bioactive substance content in albino fruits was an adaptive molecular mechanism that responded to adverse mutations, and reflected a higher antioxidant capacity. These findings provide new insights into bioactive compounds accumulation in albino mutants, and provide experimental evidence for albino fruit as a potential nutraceutical or functional food.

Produção de alface crespa utilizando água residuária da mandioca como fonte de potássio

ABSTRACT Cassava wastewater (CWW) is a natural biofertilizer successfully tested as a source of K in long-cycle crops; however, it still needs to be better evaluated in short-cycle crops. This study aimed to evaluate the use of CWW as a source of K in the growth, yield, and physicochemical quality of curly lettuce. The study was conducted in a greenhouse, in a randomized block design arranged in a 5 × 2 + 1 factorial scheme, with four replicates. Five CWW doses were evaluated as an exclusive source of K: 0, 60, 120, 180, and 240 kg ha-1 of K2O; two forms of application: 1-total dose at planting: 2-application of 50% of the dose at planting + 50% of the dose in topdressing, and an additional treatment: 100% mineral fertilization at 120 kg ha-1 of K2O dose, using KCl as the source. The number of leaves (NL), plant height (PH), fresh mass (FM), dry mass (DM), moisture (MO), ashes (ASH), fibers (FB), lipids (LIP), proteins (PTN), and carbohydrates (CHO) contents were evaluated. All agronomic attributes of lettuce showed lower values when fertilized only with CWW than mineral fertilization. Lettuce fertilized only with CWW, depending on total or split application and dose, showed higher ASH, FB, LIP, PTN, and CHO contents reaching 4.05, 3.97, 1.75, 7.11, and 3.63%, respectively, compared to mineral fertilization. The CWW can potentially be used as a complementary K source in lettuce cultivation, as its exclusive use does not replace traditional mineral fertilization.

Effect of carbon dioxide mineralization curing on mechanical properties and microstructure of Portland cement–steel slag–granulated blast furnace slag ternary paste

To investigate the synergistic effects of steel slag (SS) and granulated blast furnace slag (GBFS) on cementitious systems during carbon dioxide (CO2) mineralization curing. Ternary paste specimens containing SS, GBFS, and Portland cement (PC) were prepared to analyze the influence of drying pre-treatment time and SS–GBFS mixing ratios on mechanical properties, CO2 fixation rate, and microstructure evolution. Results emphasize the significance of appropriate drying pre-treatment in enhancing mineralization degree and compressive strength. At 60 °C drying pre-treatment temperature, extending drying pre-treatment time up to 12 h enhances both compressive strength and CO2 fixation rate. However, beyond 12 h, a decline is observed in both parameters. Moreover, under the optimal drying pre-treatment time, the compressive strength of ternary paste specimens post-mineralization increases with higher SS content, with SS exerting a more pronounced positive effect on CO2 fixation rate and compressive strength compared to GBFS. After 4 h of mineralization curing, specimens with 50% SS and 10% GBFS demonstrate a 13.16% increase in compressive strength and a 9.16% increase in CO2 fixation rate compared to specimens with 10% SS and 50% GBFS. Microscopic test results reveal that the primary crystalline product in ternary paste specimens after mineralization is calcite, with traces of aragonite. The SS–GBFS mixing ratio has minimal effect on the type of reaction products but has a predominant influence on the total content and crystallinity of calcarea carbonica.

Khảo sát hàm lượng flavonoid tổng số và hoạt tính chống gốc tự do của cao chiết từ một số loại đậu trên thị trường Việt Nam

Total flavonoid content and DPPH free radical scavenging activity of extracts from four types of bean, such as Vigna radiata, Vigna angularis, Vigna unguiculata, and Phaseolus vulgaris, in the market have been evaluated. The extracts from Phaseolus vulgaris had significantly higher flavonoid contents than the three other ones and showed a considerable free radical scavenging effect.

Mechanical and durability performance of self-compacting mortars made with different industrial by-products as fillers

This study evaluated the performance over time of Self-Compacting Mortars (SC mortars) with different industrial by-products simultaneously; Electric Arc Furnace Dust (EAFD) and fly ash, conforming (FA) or not conforming (NcFA), from coal-fired power plant or recovery filler from hot-mix asphalt plant (RF). Different SC mortars mixes were produced; three mixes with different EAFD ratio incorporation (0 %, 10 %and 20 %) for each the other industrial by-products (FA, NcFA and RF) substituted of cement at 30 %; and additional three mixes with 60 % of cement replacement by FA and the same EAFD incorporation ratios. To study the performance over time of these SC mortars, tests such as electrical resistivity, ultrasonic pulse velocity, drying shrinkage, capillarity, compressive and flexural strength were carried out up to 91 days of curing. The SC mortar compositions was evaluated by TGA/DTA and DRX analysis. Previously, the by-products used were characterized. The EAFD incorporation showed a strong influence in SC mortars, independently of the industrial by-product used. The initial inhibition of development of hardening in SC mortars due to the presence of ZnO in EAFD was lifted over time up to some extent. The simultaneous use of different industrial-by products in SC mortars in high volume content walks towards the sustainability in the construction sector.

Purification of graphite from spent carbon cathodes through the reduction of the mechanical entrainment of cryolite using polyaluminium chloride as a flocculant

Spent carbon cathodes (SCCs) generated during electrolytic aluminum production pose significant environmental hazards. To optimize resource utilization, flotation is used as an early process for the clean recovery of valuable graphite from waste cathodes. However, the mechanical entrainment in flotation restricts the further improvement of product purity, resulting in an increase in waste gas and wastewater discharge in the subsequent purification process. In this study, to increase the fixed carbon content of flotation products, flocculant was first applied to the flotation separation of SCCs, and polyaluminum chloride (PAC) was used to flocculate cryolite with high impurity content to reduce mechanical entrainment in the flotation process. Zeta potential measurements, particle size analysis, optical microscopy, and scanning electron microscopy confirmed the agglomeration of cryolite under the action of PAC. Additionally, the DLVO (Derjaguin, Landau, Verwey, and Overbeek) theory was used to estimate the interaction energy to further explain the mechanism underlying cryolite agglomeration. Interaction energy calculation revealed that the attraction energy between particles was dominant at the PAC concentration of 2000 mg/L. Flotation experiments indicated that under a constant pulp pH of 7.8, the absence of sodium silicate, and a stirring speed of 1200rpm, mechanical entrainment decreased from 91.53 ± 1.53g/L to 69.80 ± 1.90g/L, and the carbon grade of the product increased from 83.07 ± 0.28% to 88.94 ± 0.30%. Overall, the proposed purification method provides a new approach for the efficient recovery of graphite in waste cathodes, which is of great significance for the sustainable development of graphite resources.

Mineralization of Zr-REE-Y in the Ngaoumbol iron formations, central Cameroon: Insights from petrography, mineral chemistry and whole rock geochemistry

The increasing importance of rare earth elements (REE) and critical metals in contemporary society has led us to investigate the mineral potential of the Ngaoumbol area, located within Cameroon's Central African Fold Belt (CAFB). In this study, we have conducted a comprehensive analysis that includes petrography, whole rock geochemistry, and mineral chemistry, along with the application of an outlook coefficient known as Koult, with the aim to evaluate the prospectivity of the Ngaoumbol iron formations as a potential source of REE-Y resources. This coefficient is defined as the ratio of the relative abundance of critical Rare Earth Elements to the relative abundance of excess REE. The iron formations in the Ngaoumbol area are fine-to medium-grained foliated rocks with alternating magnetite and actinolite bands and quartz bands, suggesting a sedimentary parentage. These rocks have an average REE-Y content of 1438.43 ppm and a Koult of 0.77, indicating their potential as raw sources for REE. Furthermore, the investigated samples exhibit high average Zr content (7748 ppm), suggesting that the rocks may host potentially economic Zr ore. The Zr-REE-Y mineralization in the Ngaoumbol area is hosted in detrital zircon, monazite and xenotime, probably deriving from the weathering of alkaline/subalkaline rocks surrounding the deposits. Our findings suggest that the Ngaoumbol area has promising REE and Zr resources. However, further exploration and evaluation are necessary to determine the extent and economic viability of these resources.

Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations

Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.

Optimal growth conditions to enhance Chlorella vulgaris biomass production in indoor phyto tank and quality assessment of feed and culture Stock

Commercial microalgae cultivation is a dynamic field with ongoing efforts to improve efficiency, reduce costs, and explore new applications. We conducted a study to examine how different light exposure periods affect Chlorella vulgaris's growth. We employed a Phyto tank batch system of approximately 3.5 liters with LED light control, controlled airflow, and sterilized bags, maintained at 22.0 ± 2.0°C indoors. Various methods, including spectrophotometry, and cell counter were employed to monitor Chlorella vulgaris growth under different light exposure cycles. Additionally, quality analysis as feed source was employed by proximate, amino acid, beta-glucan, and microbial content analysis. The results revealed significant variations in C. vulgaris biomass production based on light exposure duration. Notably, the 16:8-hour light-dark photoperiod exhibited the highest biomass concentration, reaching 6.48 × 107 ± 0.50 cells/mL with an optical density (OD) of 1.165 absorbance at 682 nm. The 12:12-hour light-dark photoperiod produced the second-highest biomass concentration, with 2.305 × 106 ± 0.60 cells/mL at an OD of 0.489. Proximate analysis of dry algae powder revealed low lipid content (0.48%), high protein content (37.61%), variable ash concentration (average 10.75%), and a significant carbohydrate fraction (51.16%) during extended daylight and shorter dark periods. Amino acid analysis identified nine essential amino acids, with glutamic acid being the most abundant (17.7%) and methionine the least (0.4%). Furthermore, quality analysis and microbiological assays demonstrated that the C. vulgaris biomass is well-suited for fish and livestock use as a feed source and possibility as human nutraceuticals. These findings can be considered more environmentally friendly and ethically sound due to the absence of genetic modification.

Exploring the application of hydrazine hydrate in protonic ceramic fuel cells: Strategies and performance improvements

Hydrazine hydrate, characterized by its high hydrogen content and eco-friendliness, garners extensive attention in the field of fuels. Its high energy density and rapid decomposition capabilities render it a promising fuel option for Proton-Conductor Solid Oxide Fuel Cells (PCFCs). Given the unique properties of hydrazine hydrate, this study employs a dendritic porous anode structure to facilitate faster fuel transport. Additionally, the influence of operational temperature and concentration of hydrazine hydrate as fuel on cell performance is analyzed. An effective vapor management strategy is implemented to address its decomposition into water vapor, resulting in a significant 23.17 % increase in the maximum power density (MPD) of the cell. To further enhance the performance of hydrazine hydrate fuel in PCFCs, a Pt–Al spherical catalyst is integrated within the anode structure of the cell. This approach notably improves the cell performance, yielding an additional 14.98 % increase in MPD. This design not only augments the electrical output but also effectively mitigates the degradation of the interface between the anode and the electrolyte. These optimization measures significantly improve the lifespan and reliability of the cell. The outcomes of this study demonstrate the practical feasibility of using hydrazine hydrate as a fuel in PCFCs.

Enhancement the linear/nonlinear optical and magnetic properties of ZnCo2O4 nanostructures through Ni/Fe dual doping

Nano Zn0.9Ni0.1Co2-xFexO4 samples were prepared using the hydrothermal procedure in order to enhance the functional characteristics of ZnCo2O4 sample. The structural and microstructural parameters of the prepared samples were determined through Rietveld and FTIR analyses. The morphologies and compositions of the samples were examined by SEM and EDS techniques. Upon Ni2+ doping, the bandgap energy of nano ZnCo2O4 decreased from 2.22 eV to 3.61 eV–2.18 eV and 3.51 eV. Doping with Fe caused a slight decrease in bandgap energy for x = 0.05, then an increase for a higher content of Fe. Several empirical equations were used to determine the refractive index from the corresponding obtained optical energy gap values. As the Fe content rose to 5 %, the value of refractive index and nonlinear optical parameters increased; thereafter, it declined with additional Fe doping. The photoluminescence technique was used to analyze defects and processes of charge carrier recombination in the samples. ZnCo2O4 sample exhibits paramagnetic performance. With the addition of Ni to ZnCo2O4, the magnetic characteristics of the sample became superparamagnetic. Samples doped with Ni and various quantities of Fe exhibit a weak ferromagnetic behavior. The effect of doping on the magnetic transition, coercivity, saturation magnetization and remanent magnetization was explored.

Chemical characteristics and health risk evaluation of natural waters in the Du River Source National Nature Reserve: A case study in Zhushan County, Hubei Province, China

To study the distribution of trace elements in natural water of the Du River Source National Nature Reserve and to assess the water quality and health risks, Zhushan County in Hubei Province was selected as the study area. Element content in 361 natural water samples collected from Zhushan County were measured by ICP-MS, ICP-OES, and HG-AFS. The main anions and cations present in water samples from Zhushan County are Ca2+ and HCO3−. The water chemistry is predominantly influenced by the weathering of carbonate rocks. The water samples with high content of selenium (Se) (0∼82.9 μg/L, mean 4.6 μg/L) in natural water in Zhushan County are mainly distributed in the northern part of Zhushan. The strontium (Sr) content of 49.6% of the water samples (0.001–2.177 mg/L, mean 0.234 mg/L) reached the criteria of natural mineral water for drinking in China (Sr ≥ 0.2 mg/L), which is distributed throughout the county. The high content of metasilicic acid (H2SiO3) (0.026–35.910 mg/L, mean 12.598 mg/L) and zinc (Zn) (0∼407.218 μg/L, mean 12.406 μg/L) are concentrated in northern Zhushan County. 99.7% water samples were freshwater and 98.9% meet the criteria of “good” water quality. All of the natural water samples have low health risk and low heavy metal pollution. 6.1% water samples meet the criteria of Se-type mineral water, while 45.4% meet the criteria of Sr-type mineral water, and 4.4% water samples meet the criteria of “low sodium, high Se, and high Sr” mineral water. Zhushan County has the potential for Se-type mineral water and Sr-type mineral water development. The findings of this study hold immense significance for the public health implications of drinking water in Du River Source, thereby offering valuable insights for effective water resources management.

Alkanediol–hexafluoroisopropanol amphiphilic supramolecular solvent: Fabrication, characterization, and application potential for doping control of hormones and metabolic modulators in human urine

A novel amphiphilic supramolecular solvent (SUPRAS) was developed by hexafluoroisopropanol (HFIP)-induced self-assembly of 1,2-hexanediol in an aqueous matrix. Its application potential in multiclass determination was assessed by extracting from urine 10 prohibited hormones and metabolic modulators (log P from 2.38 to 7.57) listed by the World Anti-Doping Agency (WADA). This SUPRAS is stably formed across a wide pH range (2–12) and is unaffected by salt concentrations (<10 % g mL−1 of NaCl), and it has a higher density than water. The dehydration and hydrogen bonding ability of HFIP, as well as hydrophobic interactions between alkanediols, dominates the formation of the SUPRAS. The nanostructure of the SUPRAS, composed of 1,2-hexanediol, HFIP, and a high content of water (>25 %, w/w), forms large spherical micellar aggregates (10–100 μm) that exhibit amphiphilic properties. A 1,2-hexanediol-HFIP SUPRAS-based liquid-phase microextraction (LPME) method for detection of selected prohibited drugs in urine coupled with liquid chromatography–tandem mass spectrometry (LC-MS/MS) was established, optimized, and validated. The method showed fast extraction time (30 s of vortex) and low solvent consumption (160 μL of organic solvent). Under the optimal conditions (7 % g mL−1 of 1,2-hexanediol, 10 % v/v of HFIP), the SUPRAS achieved high extraction efficiencies (>80 %) and an enrichment factor of 6.0, with minimal matrix effects for all analytes due to its high amphiphilicity and large surface area. The method demonstrated excellent linearity (R ≥ 0.9909), with method limits of detection ranging from 0.018 to 0.18 ng mL−1 and method limits of quantitation ranging from 0.060 to 0.60 ng mL−1, which are well below the minimum required performance levels set by authorities. Furthermore, the intra- and inter-day relative recoveries for spiked human urine samples varied in the ranges of 90.3 %–121 % and 88.1–119 %, respectively, with relative standard deviations less than 13 % and 19 %, respectively. This SUPRAS-based LPME method proves to be simple, highly efficient, and eco-friendly, and it has promising potential for multiclass determination in doping control.

Herbal Anti-fungal Cream: A Natural Approach to Onychomycosis Treatment

Abstract: Onychomycosis is a frequent fungal infection of the nails that is difficult to cure and is not well controlled by standard medications, therapeutic options are limited. Herbal remedies have drawn interest as a result of this due to their potential as complementary therapies. The composition and effectiveness of a novel herbal anti-fungal cream that combines the powerful anti-fungal qualities of clove oil, turmeric, and pure peppermint are examined in this abstract. The strong anti-fungal properties of clove oil's high eugenol content are enhanced by curcumin, an anti-inflammatory and anti-microbial compound found in turmeric. Pure peppermint contributes antifungal and calming qualities as well as a pleasant element. The combination of these components offers a viable way to cure onychomycosis, possibly more effectively than conventional therapies because it is allnatural.

Studies of the Carbohydrates Content in Tissues of Hamster (Mesocricetus Auratus) Infected with Hookworm (Ancylostoma Ceylanicum)

Hookworm infections are the helminthic infection that affects tropical and subtropical areas. Several laboratory animals have been infected with hookworms. Due to parasitic infection, the structure and function of tissues undergo severe derangements and metabolic disorders. In the present study, a hamster, Mesocricetus auratus was infected with a hookworm, Ancylostoma ceylanicum. Investigation of the Carbohydrate content of A.ceylanicum in M. auratus was studied in control and infected ones. Understanding the level of pathogenicity caused by an A. ceylanicum infection can be aided by examining the total amount of carbohydrates present in the tissues of the host, M. auratus. A biochemical estimation of the total carbohydrate content was made. The brain and spleen were found to have higher carbohydrate contents. There was decreased carbohydrate activity in the kidney, liver, gut, and muscle. The present study depicted the alterations in the total carbohydrate content in various tissues of the host, M. auratus due to the infection of A. ceylanicum. The result of the data obtained in the various tissues due to infection was found to be statistically significant. It was further plotted graphically plotted and interpreted. The research work done in the present study helped to understand the biochemical changes taking place in the host, Mesocricetus auratus due to hookworm, Ancylostoma ceylanicum infection.

Sensory evaluation of pumpkin flour-wheat flour composite cookies: Exploring nutritional enrichment and consumer acceptance

The study comprehensively evaluated the consumer acceptability and sensory characteristics of cookies made from varying ratios of pumpkin flour and wheat flour. The researchers incorporated pumpkin flour at four different levels - 0%, 40%, 50%, and 80% - into the cookie formulations. To assess the sensory profile of these cookie samples, the researchers conducted a thorough evaluation using a 7-point hedonic scale. This scale allowed panelists to rate their level of liking or disliking for key sensory attributes, including color, aroma, taste, aftertaste, and texture. The results of this sensory analysis revealed some interesting trends. As the substitution level of pumpkin flour increased, there was a significant decrease in consumer preference for the color, aroma, and taste of the cookies. However, an intriguing finding was that the aftertaste was actually improved with higher pumpkin flour content. Notably, the cookies made with a 50% pumpkin flour to 50% wheat flour ratio were most preferred by panelists in terms of texture. This suggests that a balanced approach to the flour blend, incorporating a considerable amount of pumpkin flour, can produce cookies with an acceptable and even desirable textural profile. The study demonstrates the potential of pumpkin flour as a nutritious ingredient that can be incorporated into cookie production. The nutritional benefits of pumpkin flour, such as its high content of beta-carotene, fiber, and other vitamins and minerals, make it an attractive alternative to traditional wheat flour. However, the researchers caution that higher substitution levels of pumpkin flour, beyond the 50% ratio, may require further optimization to enhance the overall sensory acceptance of the cookies. Finding the right balance between nutritional enhancement and maintaining desirable sensory attributes will be crucial for the successful development of pumpkin flour-based cookies that appeal to health-conscious consumers.

Comparative study of Scleromitrion diffusum and Oldenlandia corymbosa: Microscopy, TLC, HPLC, and antioxidant activity.

Quality control of herbal medicines is crucial, especially the role of herbal drug identification. This is essential for preventing the misuse of herbs, which can affect efficacy or cause toxicity. Scleromitrion diffusum is a common herb, yet it is often mistaken for Oldenlandia corymbosa. This study analyzed the morphology, microscopy, thin-layer chromatography (TLC), and high-pressure liquid chromatography (HPLC) using two markers, asperuloside and scandoside methyl ester, to distinguish between S. diffusum and O. corymbosa with the analysis included 10 samples of S. diffusum and 10 samples of O. corymbosa collected from the Taiwan market. By quantifying the total polyphenols and flavonoids, we investigated the antioxidant activity, including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) scavenging effect, and reducing power to further elucidate the biological effects of the two herbs. The results of this study revealed notable differences in microscopy and suggested a TLC method for distinguishing between the two herbs in the market. In HPLC, the ratios of asperuloside and scandoside methyl ester differed between the two herbs. S. diffusum contained a higher asperuloside content. In contrast, O. corymbosa contained higher concentrations of scandoside methyl esters. With more total polyphenols and flavonoids in S. diffusum than those in O. corymbosa, the antioxidant activity of S. diffusum was superior to that of O. corymbosa. This study provides a comprehensive understanding for the identification and quality evaluation of S. diffusum in the market. RESEARCH HIGHLIGHTS: The study consolidates and clarifies the morphological and microscopic differences between Scleromitrion diffusum and Oldenlandia corymbosa - a common adulterant species of S. diffusum on the Taiwan markets. Using Asperuloside and Scandoside methyl ester as two chemical markers, the study proposes a TLC method for rapidly testing S. diffusum and O. corymbosa on the market. Through HPLC analysis, our results showed that S. diffusum and O. corymbosa had a clear difference in the ratio of two markers, Asperuloside and Scandoside methyl ester: Asperuloside/Scandoside methyl ester in S. diffusum is higher than that in O. corymbosa. Through phytochemicals contents, including total phenols content, flavonoids content, and antioxidant activity, including DPPH, ABTS•+ scavenging activity, and reducing power, S. diffusum showed slightly higher levels of phenols and flavonoids as well as a better antioxidant activity than O. corymbosa.

Creation of Single Cell Protein-Producing Mutants of Phaffia Rhodozyma

Recently, there has been an increasing demand for sustainable protein sources for aquaculture. In aquaculture, sustainable protein includes sources that can be produced with a minimal environmental footprint, such as single-cell proteins, proteins from algae, insects, and by-products from the agricultural industry. Single-cell proteins that are derived from microorganisms show a sustainable alternative to traditional protein sources due to their high protein content and rapid growth. This research focuses on creating mutants of the yeast Phaffia rhodozyma to increase single-cell protein production. This yeast has significant industrial potential and biological features. The yeast Phaffia rhodozyma is already known for its unique ability to synthesize astaxanthin, so this case will focus on its ability to produce protein. In the study, we used wild strain yeast and previously obtained white mutants on inorganic nitrogen media for further random mutagenesis methods to introduce specific mutations into the genome aimed at improving their ability to biosynthesize protein. Amino acid inhibitor was used to select potential protein-producing mutants. The protein content of the biomass obtained from the experiments was analyzed in detail using BCA (Protein Assay Reagent) analysis, which confirmed their potential as a source of nutrients for aquaculture. This method is based on the reaction between bicinchoninic acid and the peptide bonds of proteins, which results in the formation of a color whose intensity is proportional to the amount of protein in the sample. The results obtained allow further research into the development of cost-effective and environmentally friendly alternatives for feed in aquaculture.