Abstract Active stabilization of neoclassical tearing modes (NTMs) is critical for high beta plasma operation in the KSTAR tokamak. In recent device operation, an experiment was conducted to develop a m/n = 2/1 NTM stabilization in high normalized beta (βN) plasmas having βN > 3 by using electron cyclotron current drive (ECCD). The experiment is designed to first demonstrate the direct mode stabilization effect of the device EC system by varying the ECCD deposition location around the mode rational surface to prepare for future NTM stabilization in KSTAR using feedback schemes. In the experiment, the toroidal magnetic field strength, BT, is reduced to 1.5–1.6 T to create a highly localized ECCD profile on a large island width expected to produce a high stabilization effect. To align the ECCD with the mode, BT is varied between discharges by keeping the EC wave injection angles fixed to move the current deposition location across the q = 2 mode rational surface in ~1 cm steps along the plasma midplane. The result shows a prompt reduction of the mode amplitude by ~60% with a partial recovery of the loss of stored energy when the ECCD with 0.7 MW power is applied promptly after the mode onset at BT = 1.54 T. This abrupt reduction of the mode amplitude is significantly weaker or disappears in other discharges having slightly different BT in which the ECCD is deposited a few centimeters away. This result indicates a direct interaction between the driven EC current and the radially localized island structure first observed in the device. The EC ray-tracing analysis using the TORAY code shows that the applied EC-driven current aligns with the mode rational surface when the mode amplitude is observed to decrease. The stability of the observed 2/1 NTM is examined by constructing the modified Rutherford equation (MRE). The calculated EC power requirement for complete mode stabilization by assuming perfect alignment of ECCD on the mode is 0.8-1.7 MW by considering the uncertainties in the equation. This MRE-computed mode stability agrees with the experiment. The result projects that the present KSTAR EC system can stabilize the 2/1 NTM disrupting high βN plasmas, and provides the requirement of the mode-ECCD alignment for complete mode stabilization in future experiments in which an accurate alignment is planned to be made by feedback schemes being developed.