High Antioxidant Properties Research Articles

Association between dietary vitamin E and osteoporosis in older adults in the United States

BackgroundIncreased oxidative stress due to aging can lead to increased bone loss. The most abundant form of vitamin E, namely α-tocopherol, has high antioxidant properties and biological activity; however, its effect on osteoporosis has not been well studied in humans. We aimed to investigate the association between dietary vitamin E (α-tocopherol) and osteoporosis among older adults in the United States.MethodsThis cross-sectional study analyzed data on older adults in the United States aged ≥50 years from the 2007–2010, 2013–2014 and 2017–2020 pre-pandemic cycles of the National Health and Nutrition Examination Survey. Sample-weighted multivariate regression models were used, with adjustments for relevant confounders.ResultsThis study comprised 5,800 individuals with available data on dietary intake and bone mineral density of hip and spine. The mean participant age was 61.4 (standard deviation, 8.7) years, and approximately 9.9% had osteoporosis. High vitamin E intake was significantly associated with a reduced risk of osteoporosis (odds ratio, 0.96, 95% confidence interval, 0.93–0.98). In addition, there was evidence of interaction between dietary vitamin E and prior fracture on preventing osteoporosis.ConclusionsOur study indicated a linear association between dietary vitamin E levels and osteoporosis in an older population in the United States. Further research is required to explore the potential effects of different forms of vitamin E on osteoporosis.

Trehalulose: Exploring its benefits, biosynthesis, and enhanced production techniques

The increasing concern over sugar-related health issues has sparked research interest in seeking alternatives to sucrose. Trehalulose, a beneficial structural isomer of sucrose, is a non-cariogenic sugar with a low glycemic and insulinemic index. Besides its potential as a sugar substitute, trehalulose exhibits high antioxidant properties, making it attractive for various industrial applications. Despite its numerous advantages and potential application in various sectors, the industrial adoption of trehalulose has yet to be established due to lack of studies on its characteristics and practical uses. This review aims to provide a comprehensive overview of the properties of trehalulose, emphasizing its health benefits. The industrial prospects of trehalulose as sweetener and reducing agent, particularly in food and beverages pharmaceutical, and cosmeceutical sectors, are explored. Additionally, the review delves into the sources of trehalulose and the diverse organisms capable of producing trehalulose. The biosynthesis of this sugar primarily involves an enzyme-mediated process. Thus, these enzymes' properties, mechanisms, and the heterologous expression of genes associated with trehalulose production are explored. The strategies discussed in this review can be improved and applied to establish trehalulose bio-factories for efficient synthesis of trehalulose in the future. With further research and development, trehalulose holds promise as a valuable component across various industries.

Unlocking the Antioxidant Potential of Calendula Officinalis: A Comparative Study of Extraction Methods

Calendula officinalis which is also known as marigold is a species of plant in the daisy family that has antioxidant, antiinflammatory, cytotoxic, and antitumor properties. In this study we tried to analyze the antioxidant activity of calendula officinalis flowers extract. Three different extraction methods that are soxhlet, maceration, and ultrasonic procedures are applied to the calendula officinalis flowers and antioxidant activities for each extract is analyzed and compared. Methods: In this study, soxhlet, maceration and ultrasonic extraction methods and ethanol solvent are used to obtain calendula officinalis extracts. The antioxidant activity of the extracts with different concentration are investigated under DPPH solution. Results: DPPH radical scavenging activity for the extract obtained using soxhlet procedure is higher than the other two procedures that are maceration and ultrasonic. With increasing calendula officinalis extract concentration, it is observed that the antioxidant activity is also increased as expected. Conclusions: In conclusion, calendula officinalis flowers perform very high antioxidant property and the soxhlet method can be considered as the best method for the extraction. Further researches for the application of the extract can be studied.

Sea buckthorn and its flavonoids isorhamnetin, quercetin, and kaempferol favorably influence bone and breast tissue health.

Bone tissue and breast tissue are interrelated, as demonstrated by breast microcalcifications, breast cancer bone metastases, bone morphogenetic proteins, and Wnt signaling. In addition, osteoblasts and osteoclasts represent an important switch of tumor cell dormancy during bone metastasis. Damage to both types of tissues mentioned above is highly prevalent, especially in postmenopausal women, and manifests itself in osteoporosis and breast cancer. Sea buckthorn (Elaeagnus rhamnoides L.), a botanical drug with high antioxidant, antitumor, anti-inflammatory, immunomodulatory, and regenerative properties, has great therapeutic potential due to the unique composition of its bioactive metabolites. This review aimed to summarize the current knowledge from in vitro and in vivo studies on the effect of sea buckthorn, as well as its most widespread flavonoids isorhamnetin, quercetin, and kaempferol, on bone and breast tissue health. In vitro studies have revealed the beneficial impacts of sea buckthorn and aforementioned flavonoids on both bone health (bone remodeling, mineralization, and oxidative stress) and breast tissue health (cancer cell proliferation, apoptosis, tumor growth, and metastatic behavior). In vivo studies have documented their protective effects against disturbed bone microarchitecture and reduced bone strength in animal models of osteoporosis, as well as against tumor expansion and metastatic properties in animal xenograft models. In any case, further research and clinical trials are needed to carefully evaluate the potential therapeutic benefits of sea buckthorn and its flavonoids. Based on the available information, however, it can be concluded that these bioactive metabolites favorably affect both bone and breast tissue health.

Electrospun gelatin/tea polyphenol@pullulan nanofibers for fast-dissolving antibacterial and antioxidant applications.

Bio-based active food packaging materials have a high market demand. We use coaxial electrospinning technology to prepare core-shell structured nanofibers with sustained antibacterial and antioxidant properties. The fiber core layer was composed of gelatin and tea polyphenols, whereas tea polyphenols provide antibacterial and antioxidant properties; the fiber sheath was composed of pullulan polysaccharides with antioxidant properties. By using a scanning electron microscope, it can be seen that the diameter distribution of the prepared nanofibers was uniform and the surface is smooth; using a transmission electron microscope, it can be clearly seen that the nanofibers have a core-shell structure; Fourier Transform Infrared and X-ray diffraction analysis indicate that the nanofibers have an amorphous structure; the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging shows that nanofibers have higher antioxidant properties with the addition of tea polyphenols; antibacterial test showed that nanofibers had obvious inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli; and the nanofiber film dissolution test shows that nanofibers can be used as fast soluble active packaging. Finally, core-sheath-structured nanofibers can serve as active packaging for instant food, possessing both rapid water solubility and excellent antibacterial and antioxidant activity, making water-soluble nanofibers interesting applications in the field of food packaging.

Unveiling the Chemical Constituents and Inhibitory Roles of Extracts from Pinus Pinea L. Nut and Nutshell: A Novel Source for Pharmaceutical Antimicrobials.

Antibiotic resistance in infectious diseases has been a serious problem for the last century, and scientists have focused on discovering new natural antimicrobial agents. Pinus pinea has been used as a natural pharmacotherapeutic agent with antimutagenic, anticarcinogenic, and high antioxidant properties. In this study, GC-MS and LC-HR/MS were employed to analyze Pinus pinea L. nut and nutshell extracts. DPPH radical scavenging assay was performed to analyze the antioxidant properties of the extracts, but no activity was determined. GC-MS analysis showed that linoleic, oleic, and palmitic acids were the three most dominant fatty acids in nut and nutshell extracts, with ratios between 6.75 % and 47.06 % (v/v). LC-HR/MS revealed that the nutshell methanol extract had a higher phenolic content than other extracts, with vanillic acid (1.4071 mg/g). Antimicrobial activity assays showed that the minimum inhibitory concentrations (MIC) of the extracts varied between 5.94 and 190 mg/mL, and the most significant inhibition was seen in the nutshell methanol extract (MICs: between 5.94 and 47.5 mg/mL). Consequently, the antimicrobial activity of the extracts can be attributed to the dense fatty acids they contain, and the nutshell methanol extract showed the most potent inhibition related to the abundance of phenolic compounds in the extract.

Extending More than One Week the Shelf Life of Fresh-Cut Lettuce Using Vinegar Enriched in Bioactive Compounds Encapsulated in α-Cyclodextrins.

Fresh-cut salads are highly appreciated by consumers due to their healthy and convenient nature. Fresh-cut (FC) lettuce is one of the best-selling FC products due to its freshness and low preparation time for salads. However, FC lettuce is very perishable and, in addition, has been recently associated with severe foodborne illness alerts. Alternative natural sanitizing treatments to conventional sodium hypochlorite are needed. The antimicrobial activity of vinegar has been well-known since ancient times. In addition, bioactive compounds from plant byproducts are widely recognized for their antioxidant properties. This work aimed to evaluate the use of a novel and encapsulated vinegar powder enriched with bioactive compounds from fruit byproducts with high antimicrobial and antioxidant properties to preserve the physicochemical (titratable acidity, total soluble solids, weight loss, and color), microbial (psychrophiles, enterobacteria, lactic-acid bacteria, molds, and yeasts), and sensory quality of FC lettuce at 4 °C for up to 10 days. Small to no differences were observed in terms of physicochemical quality (≈0.1% titratable acidity; 2.3-3.3% total soluble solids; <1% weight loss) in comparison to control samples through storage. Vinegar treatments reduced by 2-4 log CFU g-1 the microbial loads after 10 days. In addition, a synergistic sensory antibrowning effect (greener and less yellowish appearance, showing -a* and -b*) between organic acids and the released polyphenols from the encapsulated plant byproduct extracts was observed. Hence, the enriched encapsulated vinegar represents an effective green alternative to conventional sanitizers to maintain the quality of FC lettuce through storage time.

Metabolite concentrations and the expression profiles of the corresponding metabolic pathway genes in eggplant (Solanum melongena L.) fruits of contrasting colors.

Eggplant (Solanum melongena L.) ranks fifth in importance among vegetable crops of the Solanaceae family, in part due to the high antioxidant properties and polyphenol content of the fruit. Along with the popular purple-fruited varieties of S. melongena, there are cultivars, the fruits of which are rich in phenolic compounds, but are white-colored due to the lack of anthocyanin biosynthesis. Determination of the amount of anthocyanins and other phenolic compounds, as well as carotenoids and sugars, is included in the assessment of the quality of eggplant fruits of commercial (technical) ripeness. In addition to antioxidant and taste properties, these metabolites are associated with fruit resistance to various stress factors. In this study, a comparative analysis of the content of anthocyanins, carotenoids and soluble sugars (sucrose, glucose, fructose) in the peel and pulp of the fruit of both technical and biological ripeness was carried out in purple-fruited (cv. Vlas) and white-fruited (cv. Snezhny) eggplant accessions of domestic selection. The peel and pulp of biologically ripe fruits of the cvs Vlas and Snezhny were used for comparative transcriptomic analysis. The key genes of the flavonoid and carotenoid metabolism, sucrose hydrolysis, and soluble sugar transport were shown to be differentially expressed between fruit tissues, both within each cultivar and between them. It has been confirmed that the purple color of the peel of the cv. Vlas fruit is due to substantial amounts of anthocyanins. Flavonoid biosynthesis genes showed a significantly lower expression level in the ripe fruit of the cv. Vlas in comparison with the cv. Snezhny. However, in both cultivars, transcripts of anthocyanin biosynthesis genes (DFR, ANS, UFGT) were not detected. Additionally, the purple fruit of the cv. Vlas accumulated more carotenoids and sucrose and less glucose and fructose than the white fruit of the cv. Snezhny. Biochemical data corresponded to the differential expression pattern of the key genes encoding the structural proteins of metabolism and transport of the compounds analyzed.

Different Combinations of Nitrogen and Carbon Sources Influence the Growth and Postbiotic Metabolite Characteristics of Lactiplantibacillus plantarum Strains Isolated from Malaysian Foods.

Postbiotic metabolites produced by Lactiplantibacillus plantarum strains isolated from Malaysian food have been extensively reported for their positive effects on health. Understanding the effects of different combinations of carbon and nitrogen sources on the growth and corresponding characteristics of postbiotic metabolites produced by different strains of L. plantarum is important for various applications. Hence, the effects of different combinations of carbon (glucose, lactose, sucrose and dextrose) and nitrogen (X-SEED Kat, X-SEED Peptone, X-SEED Nucleo Advanced, Nucel875 MG, FM888 and FM902) sources on the growth of six strains of L. plantarum (RG11, RG14, RI11, RS5, TL1 and UL4) and the functional characteristics (bacteriocin inhibitory activity, antioxidant activity and lactic acid concentration) of their respective postbiotic metabolites were investigated in this study. UL4 produced the highest viable cell population with sucrose and Nucel875 nitrogen source. The UL4 strain also produced the strongest bacteriocin inhibitory activity with dextrose and FM888 nitrogen source. In comparison, the RI11 strain produced the highest lactic acid concentration with dextrose and Nucel875 nitrogen source and the highest reducing power of RS5 and TL1 postbiotic metabolites was achieved with MRS medium. In the combination of sucrose and X-Seed KAT nitrogen source, RG14 produce the highest hydroxyl radical scavenging activity. The effects of different combinations of carbon and nitrogen sources on the viable cell population of L. plantarum strains and the respective functional characteristics of postbiotic metabolites were strain dependent. The current study also revealed that fermentation media were an important factor that greatly impacted the functionalities of postbiotic metabolites due to the presence of various bioactive compounds that contributed to high antioxidant and antimicrobial properties. The results of this study will facilitate the subsequent medium design and optimisation for the development and production of specific postbiotic metabolites produced by the respective L. plantarum strain for their applications in various industries.

Assessment of antioxidant properties in selected pigmented and non-pigmented rice (Oryza sativa L.) germplasm and determination of its association with Rc gene haplotypes

BackgroundAntioxidant properties of rice provide various health benefits due to its ability to inhibit cellular oxidation. Antioxidant content of rice is known to be linked with the pericarp pigmentation. The Rc gene of rice (Os07g0211500) codes for a basic helix-loop-helix (bHLH) protein, acting as a transcriptional factor in regulating proanthocyanidin biosynthesis. The current study was carried out to evaluate the variation of antioxidant properties in a selected panel of rice accessions and assess the possibility of using haplotypes defined based on the Rc gene to predict pericarp pigmentation and antioxidant content in rice.ResultsThirty-two rice accessions were evaluated for grain pericarp colour and antioxidant properties; total phenolic content (TPC), total flavonoids (TFC), proanthocyanidins (PAC) and radical scavenging activity (RSA). The parameters TPC, TFC and PAC showed significant positive correlation with RSA (r > 0.69; P < 0.01). The study panel showed a wide variation for antioxidant properties and rice accessions such as Sudu Heenati, Deweraddiri, Madathawalu, Masuran, Ld 368, At 311, Kalu Heenati, Bw 272-6B, Pokkali, At 362 and Wanni Dahanala exhibited profound potential with respect to antioxidant properties. Based on three-target sites previously reported as critical for the function of the coded bHLH protein (an A/C SNP at 1,353-bp, a 1-bp insertion/deletion at 1,388-bp, and a 14-bp insertion/deletion at 1,408-1,421-bp positioned in the mRNA corresponding to the exon 6 of rice Rc gene), three haplotypes were defined (H1-H3). Pigmentation of the rice pericarp could be successfully explained based on the defined haplotypes (H1 (C/G/+): red, and H2 (A/G/+) and H3 (C/G/-): white), and the H1 haplotype corresponded to a significantly (P < 0.05) higher TPC, TFC, PAC and RSA compared to the other haplotypes.ConclusionsThe studied rice accessions showed a significant variation with respect to antioxidant properties. Haplotype H1 defined based on the three-target sites in the exon 6 of Rc gene can detect rice accessions with red pigmented pericarp and high antioxidant properties effectively. Hence, its use can be recommended as an alternative to biochemical assays for screening during rice breeding programs.

Plant-Origin Additives from Boswellia Species in Emulgel Formulation for Radiotherapy Skin Care

The research objective of this study was to include plant-origin additives of the Boswellia species in the formulation of topical preparations for skin care after radiotherapy. The main factor damaging the skin during radiotherapy is the free radicals that form from water molecules and granulocytes in the inflammatory area; hence, the use of substances with antioxidant properties, including plant extracts rich in antioxidants, seems to be an alternative therapy in radiodermatitis treatment. A series of cosmetic preparations containing plant-origin additives from Boswellia species and corresponding placebo formulations were prepared. In order to assess the applicability of emulgels as oncocosmetics, their stability, physicochemical properties, rheological properties, and antioxidant capacity were determined. Somatosensory analysis was also performed. An attempt was also made to correlate the effect of plant-derived additives on the functional properties of the product determined via instrumental methods and the sensory properties. The most promising preparation was the emulgel containing the Soxhlet extract and essential oil (Em_SO) due to its high antioxidant properties compared to other preparations (% inhibition of 11.69) and polyphenol content (3.63 mg/dm3). Additionally, probands positively assessed all its features, including consistency (4.00), absorption (4.43), and hydration (4.71). The presence of significant correlations for % inhibition and polyphenols content with sensory and physicochemical characteristics of samples was indicated. There were low ratings for placebo preparations by probands, and the demonstrated correlations of odor with moisturization and distribution, oiliness and hydration with the % inhibition of the sample, and the content of polyphenols with the pH and size of the dispersed phase droplets proved the positive effect of the addition of plant-origin additives from Boswellia to the emulgel formulation on the functional and sensory properties.

Development of multilayer composite film based on protein‐polysaccharides with the addition of onion waste extracts and their impact on the shallot quality

AbstractAt present, multilayer composite film is attractive in the production of biodegradable films due to its essential film properties. The current study mainly focused on overcoming the problems of poor water barrier and mechanical strength observed in monolayer films (SA‐CMC and G/SA‐CMC) made from gluten (G), sodium alginate (SA), and carboxymethylcellulose (CMC). The film was developed with the addition of onion waste extracts (OWEs), primarily from peel and stalk, to enhance functional properties. The multilayer composite film consists of three layers: an outer layer made of G, an inner layer made of SA, CMC, and a middle layer made of G/SA‐CMC. The developed composite film exhibited improved physical properties (thickness: 0.348–0.629 mm and moisture: 20.889%–21.403%), mechanical properties (tensile strength: 21.943–20.640 MPa), and barrier properties (WVP: 0.212–0.516 g/m.s.Pa × 10−14) compared to monolayer films (SA‐CMC and G/SA‐CMC). The addition of OWEs enhanced the multilayer film's phenolic (20.076 and 36.175 mgGAE/g) and antioxidant activity (73.850% and 42.667%). The study found that the multilayer control film had minimal changes in weight loss (9.75% ± 0.18%) and firmness (6.68 ± 0.64 N) compared to OWEs‐added films (9.93% ± 0.49–10.02% ± 0.14% and 6.61 ± 0.37 to 6.64 ± 0.18 N). However, fresh‐peeled shallot onion packed with control film exhibited higher bacterial counts (6.70 ± 0.42 CFU/g) and yeast and mold counts (5.08 ± 0.20 CFU/g) than those packed with the OWEs‐added film (6.49 ± 0.28–6.56 ± 0.27 CFU/g and 4.75 ± 0.18–4.89 ± 0.21 CFU/g), indicating that OWEs protect the onions from microbial degradation. Overall, the multilayer control film (without OWEs) showed better results for storing the fresh‐peeled shallot onion at 4°C for 21 days.Highlights Onion waste extracts have potent high antioxidant and antimicrobial properties Multilayer film's physical, mechanical, barrier, and functional properties improved Addition of onion waste extracts increased the film's antimicrobial activity Control film showed a better effect for storing fresh‐peeled shallot onion Developed film can store fresh‐peeled shallot onion for 21 days at 4°C.

Beta-cyclodextrin–Phyllanthus emblica emulsion for zinc oxide nanoparticles: Characteristics and photocatalysis

Abstract This study presents a new approach for producing zinc oxide nanoparticles (ZnONPs) utilizing an emulsion of beta-cyclodextrin (β-CD) and Phyllanthus emblica fruit extract. When synthesized, the particles were around 90 ± 5 nm in size. The zeta potential investigation yielded a value of −27.1 mV. The generated ZnONPs exhibited 83.70% anti-oxidant activity as well as good radical scavenging capabilities. The catalytic activity of ZnONPs was investigated using rhodamine B dye, and they displayed that maximum photocatalytic degradation was found to be 90.32% when exposed to UV light and 50.5% when not exposed to UV light. The kinetic investigation of photocatalytic degradation employs pseudo-first-order rate kinetics, with a rate constant of 2.5 × 10−2 under UV irradiation and 1.1 × 10−2 without irradiation. Anti-bacterial tests against Escherichia coli bacteria demonstrated the particles’ anti-bacterial characteristics. Anti-cancer studies were carried out using KB-31 cancer cells at varying particle concentrations. The studies confirmed that the particles produced are anti-cancer. In conclusion, this study reports a new green synthesis method to fabricate ZnONPs with high anti-microbial, anti-oxidant, and anti-cancer properties and suitable for various biomedical applications.

Assessment of Nutritional, Physiochemical, Antioxidant and Sensory Properties of Seasoning Powder Containing Asplenium unilaterale Lam

The current study used Asplenium unilaterale Lam. with a natural alternative to monosodium glutamate (MSG) , along with plant mixtures to develop a seasoning powder. Four formulations (2-8 w/w of A. unilaterale Lam.) were characterized. Furthermore, the nutritional, physiochemical, amino acid profiles, antioxidant, and sensory properties were determined. The ash and carbohydrate content differed significantly among the four seasoning powders containing different concentrations of A. unilaterale Lam. The protein content significantly increased in seasoning powders as the A. unilaterale Lam. content increased.. The developed products had a water activity range of 0.33–0.40, and the color value ranges, L*, a*, and b*, were 60.42–78.38, 1.14–4.53, and 8.49–13.81, respectively. High concentrations of the non-essential amino acids aspartic acid and glutamic acid were observed, causing an umami taste that increased with A. unilaterale Lam. content. The highest antioxidant properties were measured in the sample with added A. unilaterale Lam. content. The sensory scores in taste for Sample D (8 w/w of A. unilaterale Lam.) received the highest scores, but the overall acceptability of the samples did not significantly vary. Therefore, this seasoning powder potentially has flavor and nutritional properties that provide health benefits and could be used as an alternative plant-derived seasoning product.

Preparation and characterization of peptide-modified core-shell fibrous substrates with UV-blocking properties for corneal regeneration applications

Effective UV protection is a key aspect of substrates directly exposed to UV radiation. Therefore, in the present study, fibrous substrates of core–shell morphology (PCL-core, PVP-shell) containing peptides based on tryptophan, tyrosine and cysteine (W6, YYC2 and YYC3) were prepared. Spectrophotometric studies showed UV absorption by peptides containing tyrosine and tryptophan in the UVB (up to 80%) and UVA (up to 40%) ranges. Cysteine, in turn, contributed to high antioxidant properties, confirmed by DPPH assay. The presence of peptides contributed to a nonwoven fabric characterized by the ability to absorb UV radiation and prevent the occurrence of oxidative stress (caused by the presence of free radicals). In turn, the increase in the surface zeta potential of the nonwoven after UV irradiation and higher thermal stability (demonstrated by DSC studies) indicated the crosslinking of the PVP layer under UVR, which further contributes to the increased protection of the nonwoven against its effects. In summary, obtained nonwoven exhibited functional similarity to the native cornea, providing a potential solution for enhancing corneal tissue engineering and regenerative medicine applications.

Balancing Maillard reaction products formation and antioxidant activities for improved sensory quality and health benefit properties of pan baked buns

This study investigated the impact of processing temperatures (190 °C, 210 °C, and 230 °C) and durations (7 min, 10 min, and 14 min) on the formation of Maillard reaction products (MRPs) and antioxidant activities in pan baked buns. Key Maillard reaction indicators, including glyoxal (GO), methylglyoxal (MGO), 5-hydroxymethylfurfural (5-HMF), melanoidins, and fluorescent advanced glycation end products (AGEs) were quantified. The results demonstrated significant increases in GO, MGO, 5-HMF contents (p < 0.05), and antioxidant activities (p < 0.05) when the buns were baked at 210 °C for 14 min, 230 °C for 10 min and 14 min. However, the interior MRPs of baked buns were minimally affected by the baking temperature and duration. Prolonged heating temperatures and durations exacerbated MRPs production (43.8 %–1038 %) in the bottom crust. Nonetheless, this process promoted the release of bound phenolic compounds and enhanced the antioxidant activity. Heating induces the thermal degradation of macromolecules in food, such as proteins and polysaccharides, which releases bound phenolic compounds by disrupting their chemical bonds within the food matrix. Appropriate selections of baking parameters can effectively reduce the formation of MRPs while simultaneously improve sensory quality and health benefit of the pan baked buns. Considering the balance between higher antioxidant properties and lower MRPs, the optimal thermal parameters for pan baked buns were 210 °C for 10 min. Furthermore, a normalized analysis revealed a consistent trend for GO, MGO, 5-HMF, fluorescent AGEs, and melanoidins. Moreover, MRPs were positively correlated with total contents of phenolic compounds, ferric-reducing antioxidant power (FRAP), and color, but negatively correlated with moisture contents and reducing sugars. Additionally, the interaction between baking conditions and Maillard reactions probably contributed to enhanced primary flavors in the final product. This study highlights the importance of optimizing baking parameters to achieve desirable MRPs levels, higher antioxidant activity, and optimal sensory attributes in baked buns.

Determination of the Effect of Thymoquinone on DNA Damage in Kidney Cells Treated to High Glucose Depending on Time and Dosage by Comet Assay

The purpose of this study was to assess the anti-genotoxic potential of thymoquinone (TQ) against DNA damage in NRK-52E cells treated with high glucose using the comet assay technique single cell gel electrophoresis method. Cells were propagated by regular passages in in vitro conditions. TQ proliferative concentration (10μM) and IC25 (3rd-hour: 550 mM, 12th-hour: 240 mM, 24th-hour: 200 mM) and IC50 (3rd-hour: 760 mM, 12th-hour: 400 mM, 24th-hour: 280 mM) values for each hour of high glucose and were determined separately with MTT method. At these concentrations, the cells were divided into control(C), Thymoquinone (TQ), high glucose(G) and high glucose plus thymoquinone (GT) groups; It was incubated with the indicated substances for 3, 12, 24 hours. DNA damage was evaluated by applying the comet assay protocol and the results were calculated as DNA damage index (DDI). While DDI levels were observed to be significantly higher (p&lt;0.05) in all groups administered high glucose compared to the control, a significant decrease was determined in all groups in which TQ was added along with high glucose. It was determined that high concentrations of glucose had genotoxic effects on kidney cells, and TQ administration together with high glucose, depending on concentration and time, had a significant effect on reducing DNA damage. However, it was concluded that the application of only thymoquinone significantly increased the DDI value compared to the control, and this was a data worth investigating in future studies. Additionally, TQ inhibited DNA damage. These results demonstrated the importance of TQ against nephrotic syndrome with its high antioxidant properties.

Investigation of the Antioxidant Capacity of Taraxacum Officinale L. Leaf Extract

The aim of this study is to investigate the potent antioxidant capacities of Taraxacum officinale leaf extract. Asteraceae family, the genus Taraxacum is widespread in the Mediterranean countries, with approximately 2000 species worldwide. Taraxacum officinale, a species that stands out in phytotherapy, is a perennial plant with yellow flowers commonly found in the warm regions of Asia and Europe. Initially used in folk medicine for liver diseases, it has been observed over time that it is also used for various diseases such as dyspepsia and urinary system disorders. T. officinale contains numerous phenolic compounds contributing to antioxidant, anti-inflammatory, and antimicrobial activities. This research aimed to evaluate the antioxidant capacities of aqueous and ethanol-water extracts prepared from T. officinale leaves. Antioxidant capacities were assessed using spectrophotometric methods in DPPH, FRAP, and CuPRAC forms. The results showed that the DPPH, FRAP, and CuPRAC values were higher in the 50% ethanol extract of T. officinale leaves: 137.1 mg Trolox/g (DPPH method), 132.7 mg Trolox/g (FRAP method), and 409.9 mg Trolox/g (CuPRAC method). In conclusion, Taraxacum officinale leaves clearly demonstrate to be a rich source of polyphenols with high antioxidant properties. It has no seious side effect or toxicity. Considering its lack of serious side effects and toxicity, when formulated into herbal medicine, it could be effective in the treatment of various diseases, especially common liver and bile diseases, based on its biological activity studies.

Multi-component synthesis and invitro biological assessment of novel pyrrole derivatives and pyrano[2,3-c]pyrazole derivatives using Co3O4 nanoparticles as recyclable nanocatalyst

In this study, Co3O4 nanoparticles were used as nanocatalyst for two different series of nitrogen-containing heterocyclic compounds, including pyrrole (Pyo) derivatives and pyrano [2, 3-c]pyrazole (Pya[2, 3-c]Pyz) derivatives. In the synthesis of derivatives, using 15 mol% and 10 mol% of the catalyst for Pyo derivatives and Pya[2, 3-c]Pyz derivatives, respectively, an efficiency between 83% and 96%, were observed. In addition, novel derivatives of Pyo and Pya[2,3-c]Pyz were synthesized and their structures were confirmed. In general, the advantages of using cobalt nanoparticles compared to previous reports include the synthesis of new derivatives, lower temperature used in the synthesis of derivatives, shorter synthesis time and high efficiency. The biological properties of the synthesized products, such as antibacterial, antifungal, and antioxidant properties, were tested and investigated. In antibacterial and antifungal tests, IZD, MIC, MBC, and MFC were measured and reported. In antioxidant activity, IC50 was calculated and reported. High reusability, green and environmentally friendly, synthesis of new derivatives and synthesis of products with higher efficiency and shorter time were the important benefits of using cobalt nanoparticles as a catalyst. In antioxidant tests, the IC50 for synthesized Pyo derivatives and Pya[2, 3-c] Pyz derivatives were between 12.2 and 13.71 μg/mL, and 16.18–17.75 μg/mL, respectively. In antimicrobial testes, the MIC for synthesized Pyo derivatives and Pya[2, 3-c]Pyz derivatives were between 2 and 4,096 μg/mL, and 2–2048 μg/mL, respectively. The results showed that the antioxidant property of Pyo derivatives were more than Pya[2, 3-c] Pyz derivatives, but the antimicrobial effect of Pya[2,3-c] Pyz derivatives were more than Pyo derivatives. The antioxidant results proved that the activity of Pyo derivatives and Pya[2, 3-c] Pyz derivatives does not depend on the substitutions of the derivatives and is close to each other. Therefore, based on this, a proposed mechanism for stability of DPPH by Pyo derivatives and Pya[2, 3-c] Pyz derivatives were suggested. Finally, based on the more stable resonance structures of Pyo derivatives, compared to Pya[2, 3-c] Pyz derivatives, its high antioxidant property was justified. Pya[2, 3-c] Pyz derivatives has two heterocyclic rings connected together pyrano and pyrazole, but Pyo derivatives has only one heterocyclic ring (pyrrole). So high antimicrobial property of Pya[2, 3-c] Pyz derivatives compared to Pyo derivatives can be attributed to having two bioactive heterocyclic rings.

Synthesis of novel hydrophilic celastrol nanoformulation by entrapment within calcium phosphate nanoparticle and study of its antioxidant activity against neurotoxin-induced damage in human neuroblastoma cells

Celastrol, a pentacyclic triterpenoid found in Chinese herb Tripterygium wilfordii, is considered as one of the top-five natural medicinal compounds with high antioxidant property. However, celastrol has poor aqueous solubility and thereby low bioavailability, restricting its clinical application as drug. To overcome this problem, we nanonized celastrol by entrapping it within hydrophilic nanocarrier – calcium phosphate nanoparticle. The synthesized calcium phosphate celastrol nanoparticle (CPCN) had average size of 35 nm, spherical shape, significant stability with (−) 37 mV zeta potential, celastrol entrapment efficiency around 75 % and low celastrol release kinetics spanning over 7 days, as measured by different techniques like FESEM, AFM, DLS, and spectrophotometry. Studies on the antioxidant potency of CPCN by flow cytometry and fluorescence microscopy depicted that the toxicity developed in human neuroblastoma cells SH-SY5Y by treatment with the selective neurotoxin MPP+ iodide (N-Methyl-4-phenylpyridinium iodide) got reduced by pretreatment of the cells with CPCN. Determination of cellular ROS content, depolarization level of mitochondrial membrane potential, cell cycle analysis and nuclear damage in MPP+-exposed cells demonstrated that CPCN had about 65 % more antioxidant efficacy over that of bulk celastrol. Thus, the nanonization process transformed hydrophobic celastrol into hydrophilic CPCN, having high potentiality to be developed as an effective antioxidant drug.