Context: The COVID-19 outbreak has made working from home (WFH) the new way of working for information technology professionals. WFH can affect the well-being and productivity of employees with no remote work experience. Aim: The objective of this study is to estimate the prevalence of burnout syndrome among information technology professionals during the COVID-19 pandemic. Setting and Design: Cross sectional on line survey was done with google form. Materials and Methods: A online survey was conducted by sending a burnout questionnaire consisting of 28 questions to the information technology professionals through the Google Forms and the data were collected and analyzed. A total of 125 information technology professionals participated in this study. Statistical Analysis: Descriptive statistics and frequency distribution was done to calculate the prevalence. Results: The findings of this study revealed that 39.2% of the population had moderate stress, 30.4% of the population had high stress, and 5.6% of the population had dangerous stress. This could be because of too much work allotted to finish, repetitive and monotonous work, change in the working environment, extended working hours, too much distraction, and social isolation. Conclusion: This study concluded that there is a high amount of stress among the information technology professionals and the higher rates of work-related stress may significantly increase the incidence of other health disorders.