High-altitude Conditions Research Articles

A Deep Learning Approach for Enhanced Real-Time Prediction of Winter Road Surface Temperatures in High-Altitude Mountain Areas

Low temperatures and icing in winter are significant factors that severely affect highway safety and traffic mobility. To enhance the precision and reliability of real-time winter road surface temperature (RST) prediction, a short-term prediction model is developed that harnesses both feature selection and deep learning. Leveraging meteorological data from a mountain highway in Yunnan, China, the key environmental variables affecting road surface temperature were first extracted using a random forest (RF) model for feature selection. These features were then combined with RST data to construct multiple groups of input variable combinations for the prediction model. A short-term prediction model with a 10-minute update frequency was built using a long short-term memory neural network (LSTM), namely RF-LSTM. The best input variable combination and preset parameters for the prediction model were determined through comparative testing with prevalent machine learning models, and the transferability of the prediction model was verified. The results showed that the best input variable combination for the RF-LSTM prediction model was road surface temperature and air temperature. The model recognised that the short-term RST was affected by long and short-term memory characteristics within a two-hour timeframe. When compared to the RF model, backpropagation (BP) neural network model and the standard LSTM model, the proposed model reduces prediction errors by 59.15%, 31.10% and 20.26%, respectively, while the prediction accuracy is 99.13% within an error margin of ±0.5℃. On the verification dataset, the proposed model maintains its time transferability with an average prediction absolute error of 0.0478. In all, the proposed model not only achieves a higher level of precision in real-time RST predictions but also ensures a more consistent and reliable performance under the challenging conditions of high altitude and mountainous terrain, offering enhanced support for traffic safety and road maintenance decision-making.

Experimental Investigations on the On-Site Crack Control of Pier Concrete in High-Altitude Environments

Concrete structures in high-altitude environments face many challenges. Establishing concrete crack control methods in high-altitude environments is crucial for enhancing the service capacity of concrete structures. In this study, a multi-field (hydration-temperature–humidity-constraint) coupling model was used to quantitatively assess the cracking risk of pier bodies at high altitude. On-site crack control tests were conducted on pier bodies using a micro-expansion anti-cracking agent to demonstrate the effectiveness of deformation shrinkage compensation in crack control at high altitudes. The results indicated that there was a risk of cracking in the pier body at high-altitude conditions, especially within 0.3 m from the pile cap and ±2.5 m from the center of the pier side surface. Compared with conventional piers, the micro-expansion anti-cracking agent approximately doubled the unit expansion deflection of piers at high temperatures while reducing the unit shrinkage deflection of piers by 11% to 12% at low temperatures. The concrete in conventional pier bodies was in a tension state after long-term hardening, while the concrete treated with the micro-expansive anti-cracking agent was in compression. Therefore, the deformation compensation effect of the micro-expansive anti-cracking agent was significant and reduced the risk of concrete cracking. In addition, early freezing had a significant impact on concrete strength, underscoring the importance of effective temperature control during the early stages of concrete placement in high-altitude environments.

Transcriptome and Metabolome Analyses Reveal High-Altitude Adaptation Mechanism of Epididymis Sperm Maturation in Tibetan Sheep

In this study, the epididymal histology, caepididymal sperm physiological parameters, serum reproductive hormones, and antioxidant enzyme SOD levels of Tibetan sheep at a 2500 m and 3500 m altitude were compared by using a combination of transcriptome and metabolomics methods. This was conducted to investigate the effects of a high-altitude environment on spermatogenesis and the maturation of Tibetan sheep. The results showed that compared to the low-altitude group, the high-altitude group had a smaller epididymal lumen, thicker epididymal wall, significantly decreased sperm survival rate, and significantly increased sperm deformation rate, but no difference in sperm motility and sperm respiratory intensity. With increasing altitude, Tibetan sheep showed a decreasing trend in serum reproductive hormones (FSH and T), while the antioxidant enzyme SOD activity was significantly reduced. Transcriptomic analysis revealed 139 differentially expressed genes in the Tibetan sheep epididymis under high-altitude conditions. The SYCP2 gene is involved in multiple biological processes related to reproduction and plays an important role in the regulation of epididymal function and sperm quality in Tibetan sheep. Genes like ADCYAP1R1, CABP2, CALN1, and ATP6V1B1 can help maintain sperm viability and maturation by regulating the cAMP signaling pathway, calcium ion homeostasis, and cellular signaling. Metabolomic analysis found that the high-altitude group had increased adenosine content and decreased prostaglandin I2 content in the epididymis. These metabolites are involved in spermatogenesis, motility, fertilization, and early embryonic development. The integrated omics analysis suggests that Tibetan sheep adapt to the high-altitude hypoxic environment by regulating cAMP signaling pathway genes like ADCY and PRKACA, as well as metabolites like adenosine and prostaglandin I2, to maintain epididymal function and sperm motility. These genes and metabolites play an important role in maintaining normal epididymal function and sperm motility at high altitudes.

Comparative effect of FYM and Azotobacter on morphology and nutritional quality of high and low altitude grown knol khol (Brassica oleracea var. gongylodes L.) cultivar White Vienna

Extreme environmental conditions of high altitude (HA) expose the food crops to abiotic stresses that may impact their growth and nutritional composition, which also varies depending on agricultural practices being used. Therefore, the present study is designed to a compare the efficacy of organic agri-treatments viz. farm yard manure (FYM) and Azotobacter on morphological and nutritional traits of knol-khol cultivar White Vienna grown at HA and low altitude (LA). The field trial was conducted as a two-factorial experiment in a randomized block design. The first factor was treatments (T1-FYM, T2-Azotobacter, T3-FYM+Azotobacter and T4-(control) and second factor was cultivation locations (HA vs. LA). The findings revealed that the application of treatment T3 at HA resulted in higher total soluble solids (1.38-fold), titratable acidity (0.06-fold), total carbohydrate (1.9-fold), crude protein (3.7-fold), crude fat (3-fold) and dietary fiber content (78-fold) whereas, yield (137.6-fold) and ash content (0.85-fold) content were found higher at LA. The current study emphasizes upon the superior efficiency of combination treatment of FYM and Azotobacter at HA to improve the nutritional quality of food crops, with added benefits of environmental sustainability and nutritional security.

Whole genome re-sequencing reveals high altitude adaptation signatures and admixture in Ladakhi cattle

Ladakhi cattle, native to the high-altitude region of Ladakh in northern India (ranging from 3,000 to 5,000 m above sea level), have evolved unique genetic adaptations to thrive in harsh environmental conditions, such as hypoxia, extreme cold, and low humidity. This study explored the genome of Ladakhi cattle to investigate genetic structure, selection signatures, and adaptive mechanisms. Whole genome sequencing reads, generated on Illumina NovaSeq 6000 platform, were aligned to the Bos taurus reference genome with BWA-MEM. SNPs were identified and filtered using GATK and bcftools, and functionally annotated with SnpEff. For population genomic analysis, PCA and admixture modeling assessed genetic structure, while Neighbor-Joining trees, LD decay, nucleotide diversity (π), and FST evaluated phylogenetic relationships and genetic variation. Selective sweeps were detected using RAiSD, and gene-set enrichment and protein–protein interaction analyses were conducted to explore functional pathways related to adaptation. The study revealed 3,759,279 unique SNPs and demonstrated that Ladakhi cattle form a distinct genetic cluster with an estimated admixture of 68 % Bos indicus and 32 % Bos taurus ancestry. Key findings include rapid linkage disequilibrium decay, low inbreeding level, and the identification of selection signatures and genes associated with hypoxia response, energy metabolism, and cold adaptation. Mean nucleotide diversity (π, 0.0037) and FST values indicated moderate genetic differentiation from other breeds. The analysis highlighted selection signatures for genes like HIF1A, ENO4, ANGPT1, EPO, NOS3, MAPK3, HMOX1, BCL2,CAMK2D, MTOR, AKT2,PIK3CB, and MAP2K1, among others, including various keratin and heat shock proteins. The interaction between genes associated with hypoxia signaling (HIF-1) and other enriched pathways such as PI3K, mTOR, NFκB, ERK, and ER stress, reveals a complex mechanism for managing hypoxic stress in Ladakhi cattle. These findings offer valuable insights for breeding programs aimed at enhancing livestock resilience in extreme environments and enhance understanding of mammalian adaptation to high-altitude conditions.

Biofermentation of aquatic plants: Potential novel feed ingredients for dairy cattle production

The study assessed the impacts of aquatic plant silages on feeding efficiency and dairy cattle health as an alternative to conventional corn silage under high altitude conditions. Mid-lactation Holstein cows were assigned to treatment groups according to a randomized complete block design of parity, previous 105-d milk yield, and body weight. Cows (n = 8 per group) were fed with aquatic plant silage inoculated with Bacillus subtilis (BS), Yeast (YS), or conventional corn silage without inoculants (control) in addition to [standard grain feed] for 75 consecutive days. BS and YS had higher protein contents than control silage (111.20 ± 7.68, 112.10 ± 6.83 vs 76.94 ± 3.48 g/kg DM), while feeding efficiency was comparable between treatments (1.07, 0.99, and 0.90, respectively). In addition, the addition of aquatic plant silage in ruminant diets enhanced immunity and antioxidant capacity when compared with control group. Metagenomic analysis showed similar composition in rumen microbiota between YS and control groups, with higher enrichment for energy and nitrogen utilization pathways in YS-treated cows. This study highlights the use of aquatic plant silage as an alternative feed for dairy cattle with higher protein than corn silage. Our results suggest YS or BS could potentially boost immune and antioxidant functions, improving adaptation to high-altitudes and reducing demand for high input corn production on the Qinghai-Tibetan Plateau.

Study on the performance of catalytic reactor for aircraft fuel tank inerting system

The catalytic reactor, which plays a pivotal role in the novel aircraft fuel tank green inerting system, encounters complex and alternating inlet boundary conditions. In this study, a transient theoretical model of the catalytic reactor was established and solved through programming, and its accuracy was verified through self-built experimental setups. The dynamic performance of the catalytic reactor and the impact of operational parameters were investigated. Additionally, a method for determining the operational range of the catalytic reactor was proposed. The results indicate that the bed temperature gradually increases to 370°C along the axial direction as the reaction progresses over time. In addition, the concentrations of fuel vapor and oxygen gradually decrease along the axial direction or with time, while achieving a fuel vapor conversion rate of 70.13% under design conditions. The promotion of catalytic reactions can be achieved by lowering the inlet gas velocity, elevating temperature, or increasing the concentrations of fuel vapor and oxygen. However, the bed's maximum temperature may surpass the self-ignition temperature of fuel vapor by 425°C. A novel indicator for oxygen consumption rate is proposed, which should be comprehensively evaluated in conjunction with the conversion rate to determine the performance of catalytic reactors. Moreover, increasing the reactor diameter can enhance both conversion rate and oxygen consumption rate. Pressure drop is detrimental to the catalytic reaction, therefore, high altitude and low-pressure conditions should be considered as the standard for reactor design. Meanwhile, this paper proposes a theoretical model to determine the operational range of the catalytic reactor based on criteria such as suitable catalytic efficiency and flight temperature. The research findings presented in this study provide a solid theoretical foundation for optimizing catalytic reactor design, thereby significantly promoting the application of green inerting systems.

Assessment of the health risks associated with heavy metal contamination in the groundwaters of the Leh district, Ladakh.

There has been a significant rise in cancer-related mortality in the Ladakh region during the past 10years. The most common type of case is gastrointestinal cancer, which has been linked in theory by medical research to lifestyle factors, high altitude conditions, and the prevalence of Helicobacter pylori bacteria brought on by poor hygiene. Nevertheless, the precise cause of the rise in cancer cases is still unknown. Concurrently, there has been a significant change in Ladakh's water use practices due to development, improved basic utilities, and related vocational shifts. The local population has become increasingly reliant on groundwater since it provides a year-round, continuous water supply for home and agricultural uses. In this study, we assessed heavy metal contamination in groundwaters and associated human health risks. The results indicate that 46-96% of the groundwater samples have heavy metal pollution with a health hazard index > 1, which means using these groundwaters for drinking, food preparation, and agriculture is likely to result in carcinogenic and non-carcinogenic health hazards. The main heavy metal contaminants found in the groundwater of the Leh district include Cr, As, Hg, and U. According to the health risk assessment, 46-76% of the groundwater samples contain unsafe levels of Cr and As. Prolonged exposure to these levels is likely to cause gastrointestinal cancer in the local population. Acute to chronic exposure to U and Hg concentrations present in some groundwater samples is likely to result in various non-carcinogenic health risks.

High biological N fixation potential dominated by heterotrophic diazotrophs in alpine permafrost rivers on the Qinghai‒Tibet Plateau

Biological nitrogen (N) fixation is a pivotal N source in N-deficient ecosystems. The Qinghai‒Tibet Plateau (QTP) region, which is assumed to be N limited and suboxic, is an ideal habitat for diazotrophs. However, the diazotrophic communities and associated N fixation rates in these high-altitude alpine permafrost QTP rivers remain largely unknown. Herein, we examined diazotrophic communities in the sediment and biofilm of QTP rivers via the nitrogenase (nifH) gene sequencing and assessed their N fixing activities via a 15N isotope incubation assay. Strikingly, anaerobic heterotrophic diazotrophs, such as sulfate- and iron-reducing bacteria, had emerged as dominant N fixers. Remarkably, the nifH gene abundance and N fixation rates increased with altitude, and the average nifH gene abundance (2.57 ± 2.60 × 108 copies g−1) and N fixation rate (2.29 ± 3.36 nmol N g−1d−1) surpassed that documented in most aquatic environments (nifH gene abundance: 1.31 × 105 ∼ 2.57 × 108 copies g−1, nitrogen fixation rates: 2.34 × 10−4 ∼ 4.11 nmol N g−1d−1). Such distinctive heterotrophic diazotrophic communities and high N fixation potential in QTP rivers were associated with low-nitrogen, abundant organic carbon and unique C:N:P stoichiometries. Additionally, the significant presence of psychrophilic bacteria within the diazotrophic communities, along with the enhanced stability and complexity of the diazotrophic networks at higher altitudes, clearly demonstrate the adaptability of diazotrophic communities to extreme cold and high-altitude conditions in QTP rivers. We further determined that altitude, coupled with organic carbon and phosphorus, was the predominant driver shaping diazotrophic communities and their N-fixing activities. Overall, our study reveals high N fixation potential in N-deficient QTP rivers, which provides novel insights into nitrogen dynamics in alpine permafrost rivers.

Complete mitochondrial genome of Hippophae tibetana: insights into adaptation to high-altitude environments.

Hippophae tibetana, belonging to the Elaeagnaceae family, is an endemic plant species of the Qinghai-Tibet Plateau, valued for its remarkable ecological restoration capabilities, as well as medicinal and edible properties. Despite being acknowledged as a useful species, its mitochondrial genome data and those of other species of the Elaeagnaceae family are lacking to date. In this study, we, for the first time, successfully assembled the mitochondrial genome of H. tibetana, which is 464,208 bp long and comprises 31 tRNA genes, 3 rRNA genes, 37 protein-coding genes, and 3 pseudogenes. Analysis of the genome revealed a high copy number of the trnM-CAT gene and a high prevalence of repetitive sequences, both of which likely contribute to genome rearrangement and adaptive evolution. Through nucleotide diversity and codon usage bias analyses, we identified specific genes that are crucial for adaptation to high-altitude conditions. Notably, genes such as atp6, ccmB, nad4L, and nad7 exhibited signs of positive selection, indicating the presence of unique adaptive traits for survival in extreme environments. Phylogenetic analysis confirmed the close relationship between the Elaeagnaceae family and other related families, whereas intergenomic sequence transfer analysis revealed a substantial presence of homologous fragments among the mitochondrial, chloroplast, and whole genomes, which may be linked to the high-altitude adaptation mechanisms of H. tibetana. The findings of this study not only enrich our knowledge of H. tibetana molecular biology but also advance our understanding of the adaptive evolution of plants on the Qinghai-Tibet Plateau. This study provides a solid scientific foundation for the molecular breeding, conservation, and utilization of H. tibetana genetic resources.

Flying to high-altitude destinations.

Every year millions of people fly to high-altitude destinations. They thereby expose themselves to specific high-altitude conditions. The hypoxic environment (low ambient oxygen availability) constitutes a major factor affecting health and well-being at high altitude. While the oxygen availability is already moderately reduced inside the aircraft cabin, this reduction becomes aggravated when leaving the plane at high-altitude destinations. Especially if not pre-acclimatized, the risk of suffering from high-altitude illnesses, e.g., acute mountain sickness, high-altitude cerebral or pulmonary edema, increases with the level of altitude. In addition, diminished oxygen availability impairs exercise tolerance, which not only limits physical activity at high altitude but may also provoke symptomatic exacerbation of pre-existing diseases. Moreover, the cold and dry ambient air and increased levels of solar radiation may contribute to adverse health effects at higher altitude. Thus, medical pre-examination and pre-flight advice, and proper preparation (pre-acclimatization, exercise training, and potentially adaptation of pharmacological regimes) are of utmost importance to reduce negative health impacts and frustrating travel experiences.

The role of the heart in the evolution of aerobic performance.

Aerobic metabolism underlies vital traits such as locomotion and thermogenesis, and aerobic capacity influences fitness in many animals. The heart is a key determinant of aerobic capacity, but the relative influence of cardiac output versus other steps in the O2 transport pathway remains contentious. In this Commentary, we consider this issue by examining the mechanistic basis for adaptive increases in aerobic capacity (thermogenic V̇O2,max; also called summit metabolism) in deer mice (Peromyscus maniculatus) native to high altitude. Thermogenic V̇O2,max is increased by acclimation to cold hypoxia (simulating high-altitude conditions), and high-altitude populations generally have greater V̇O2,max than their low-altitude counterparts. This plastic and evolved variation in V̇O2,max is associated with corresponding variation in maximal cardiac output, along with variation in other traits across the O2 pathway (e.g. arterial O2 saturation, blood haemoglobin content and O2 affinity, tissue O2 extraction, tissue oxidative capacity). By applying fundamental principles of gas exchange, we show that the relative influence of cardiac output on V̇O2,max depends on the O2 diffusing capacity of thermogenic tissues (skeletal muscles and brown adipose tissues). Functional interactions between cardiac output and blood haemoglobin content determine circulatory O2 delivery and thus affect V̇O2,max, particularly in high-altitude environments where erythropoiesis can increase haematocrit and blood viscosity. There may also be functional linkages between cardiac output and tissue O2 diffusion due to the role of blood flow in determining capillary haematocrit and red blood cell flux. Therefore, the functional interactions between cardiac output and other traits in the O2 pathway underlie the adaptive evolution of aerobic capacities.

Phytochemical Characteristics of Some Grape Cultivars Grown in High Altitude Conditions at Lake Van Basin

Phytochemical Characteristics of Some Grape Cultivars Grown in High Altitude Conditions at Lake Van Basin

Meteorological regime of the Elbrus high-mountain zone during the accumulation period

Unique automated meteorological observations were carried out on the southern slope of Elbrus, near Pastukhov Rocks, at 4700 m a.s.l., during the 2021–2022 accumulation season. Data were obtained on air temperature, humidity, wind speed and direction, snowdrift and radiation fluxes with a temporal resolution of 1 minute or less. Analysis of the data series showed that the representative winter air temperature at this altitude on the southern slope of Elbrus is –10 °С, and the minimum is –36.4 °С; the partial pressure of water vapor does not exceed 3.5 hPa. At the same time, the average daily maximum of wind speed amounted 13.1 m s–1 with the absolute maximum of 54.1 m/s. Snowstorms with a snow transport intensity of more 0.1 kg/m2s–1 are quite common phenomenon in winter, while the maximum average value of the transport reaches 0.87 kg/m2s–1. An empirical relationship was established between the average hourly wind speed and the maximum gust speed for the same period, and it was shown that for these conditions the wind gust exceeds the average hourly wind speed by 1.8 times, while the representative value of the standard deviation of wind speed is 5.8 m s–1. This information may be useful not only for the glaciologic problems and modeling, but also for construction and engineering surveys, which are relevant in view of the present-day active development of the mountain ski infrastructure on the southern macro-slope of the Elbrus. In addition, the obtained series of instrumental observations were used to assess the quality of reanalysis data for high mountain regions taking as an example the ERA5. The ERA5 reanalysis was demonstrated to reproduce rather successfully the air temperature, wind speed and humidity in high mountain conditions, but extreme values for all these parameters are underestimated. Thus, the minimum temperature in winter turned out to be overestimated by 2 °C, and the maximum was underestimated by 4 °C, while the wind speed, according to the ERA5 reanalysis, never exceeded 40 m/s during the above observation period. It is also shown that the FlowCapt4 acoustic blizzard gauge (driftometer) can be used to estimate average wind speeds since it is less sensitive to severe high-altitude conditions compared to acoustic and cup anemometers.

The State of Lipid Metabolism in Old Rats in High Altitude Conditions Against the Background of the Use of Coenzyme Q10

The problem of population aging is relevant both in Kyrgyzstan and in the global space. At the same time, the preservation of the functional capabilities of the body of elderly people living in adverse climatic conditions is of particular importance. 20-30% of the inhabitants live in the mountainous area of Kyrgyzstan at an altitude of 2000-4000 meters above sea level. It is known that in high-altitude conditions, the body is influenced by extreme factors of the highlands, which accelerate the aging process and reduce life expectancy. This article presents data from experimental studies of energy metabolism disorders in old rats and its correction of coenzyme Q10 at an altitude of 3200 m above sea level. It was found that in old rats, under conditions of short-term adaptation in the mountains, an increase in the level of total cholesterol, triglycerides and low-density lipoproteins was shown, and the level of high-density lipoproteins decreased, indicating a violation of lipid metabolism. Also, due to oxidative stress in high-altitude conditions, the level of malondialdehyde, superoxide dismutase and catalase in the blood of animals was increased. Oral administration of coenzyme Q10 at a dose of 30 mg/kg of body weight 1 time per day for 30 days to old rats led to a decrease in total cholesterol, low-density lipoproteins, triglycerides and an increase in high-density lipoproteins, due to the fact that coenzyme Q10 protects the plasma membrane of the cell from lipid peroxidation, preventing oxidative modifications lipids, which leads to a reduced risk of coronary heart disease, heart attack and stroke.

Experimental and numerical simulation of the attenuation effect of blast shock waves in tunnels at different altitudes

Traffic engineering such as tunnels in various altitudinal gradient zone are at risk of accidental explosion, which can damage personnel and equipment. Accurate prediction of the distribution pattern of explosive loads and shock wave propagation process in semi-enclosed structures at various altitude environment is key research focus in the fields of explosion shock and fluid dynamics. The effect of altitude on the propagation of shock waves in tunnels was investigated by conducting explosion test and numerical simulation. Based on the experimental and numerical simulation results, a prediction model for the attenuation of the peak overpressure of tunnel shock waves at different altitudes was established. The results showed that the peak overpressure decreased at the same measurement points in the tunnel entrance under the high altitude condition. In contrast, an increase in altitude accelerated the propagation speed of the shock wave in the tunnel. The average error between the peak shock wave overpressure obtained using the overpressure prediction formula and the measured test data was less than 15%, the average error between the propagation velocity of shock waves predicted values and the test data is less than 10%. The method can effectively predict the overpressure attenuation of blast wave in tunnel at various altitudes.

Assessment of metabolomic variations among individuals returning to plain areas after exposure to high altitudes: a metabolomic analysis of human plasma samples with high-altitude de-acclimatization syndrome.

High altitude de-acclimatization (HADA) is gradually becoming a public health concern as millions of individuals of different occupations migrate to high-altitude areas for work due to economic growth in plateau areas. HADA affects people who return to lower elevations after exposure to high altitudes. It causes significant physiological and functional changes that can negatively impact health and even endanger life. However, uncertainties persist about the detailed mechanisms underlying HADA. We established a population cohort of individuals with HADA and assessed variations in metabolite composition. Plasm samples of four groups, including subjects staying at plain (P) and high altitude (H) as well as subjects suffering from HADA syndrome with almost no reaction (r3) and mild-to-moderate reaction (R3) after returning to plain from high altitude, were collected and analyzed by Liquid Chromatography-Mass Spectrometry metabolomic. Multivariate statistical analyses were used to explore significant differences and potential clinical prospect of metabolites. Although significantly different on current HADAS diagnostic symptom score, there were no differences in 17 usual clinical indices between r3 and R3. Further multivariate analyses showed isolated clustering distribution of the metabolites among the four groups, suggesting significant differences in their metabolic characteristics. Through K-means clustering analysis, we identified 235 metabolites that exhibited patterns of abundance change consistent with phenotype of HADA syndrome. Pathway enrichment analysis indicated a high influence of polyunsaturated fatty acids under high-altitude conditions. We compared the metabolites between R3 and r3 and found 107 metabolites with differential abundance involved in lipid metabolism and oxidation, suggesting their potential role in the regulation of oxidative stress homeostasis. Among them, four metabolites might play a key role in the occurrence of HADA, including 11-beta-hydroxyandrosterone-3-glucuronide, 5-methoxyindoleacetate, 9,10-epoxyoctadecenoic acid, and PysoPC (20:5). We observed the dynamic variation in the metabolic process of HADA. Levels of four metabolites, which might be provoking HADA mediated through lipid metabolism and oxidation, were expected to be explore prospective indices for HADA. Additionally, metabolomics was more efficient in identifying environmental risk factors than clinical examination when dramatic metabolic disturbances underlying the difference in symptoms were detected, providing new insights into the molecular mechanisms of HADAS.

Cold start performance of diesel engine at extremely low ambient temperatures and high altitudes: The superiority of premixing diethyl ether

The cold-start performance poses a primary challenge influencing the reliability of diesel engines operating in plateau and low-temperature regions. An experimental investigation was conducted to assess the cold-start performance of diesel engines with premixing diethyl ether (DEE), spanning altitudes from sea level to 4000 m and ambient temperatures of −20 °C and −40 °C. The diesel engine with premixing DEE exhibited successful cold starts at all these test conditions. In comparison, the diesel engine employing intake heating failed to reach idle speed in −40 °C high-altitude conditions. The premixed DEE could generate radicals, accompanied by a minor heat release preceding the injection of diesel, facilitating ignition. Furthermore, the premixed DEE enhanced fuel evaporation and facilitated the formation of combustible mixtures with higher equivalence ratios at low temperatures. Moreover, the fuel film resulting from wall impingement with premixing DEE was thinner than that with intake heating, contributing to decreased incomplete combustion losses. Energy analysis results revealed that the diesel engine employing premixing DEE demonstrated higher indicated thermal efficiency. The cold start assistance by premixing DEE proved to be much more effective, and the test engine could start smoothly under extremely low temperatures of −40 °C and high altitudes up to 4000 m.

Contents of IL-1β and IL-10 in Blood Plasma Dynamics in Experimental Animals during Simulation of Wound Process of Soft Tissue in Low Altitude Conditions and During the Period of Deadaptation to High Altitudes

Presents the dynamics of the content of IL-1β and IL-10 in the blood plasma of experimental animals when modeling a wound process in low-altitude conditions and during the period of deadaptation to high-altitude conditions. The animals were divided into 3 series: Series I — control (permanently living in the conditions of Bishkek); Series II — experimental, after a 3-day stay at the experimental high-mountain base of KSMA named after. A. A. Raimzhanov as part of the Central Research Laboratory (moved to the conditions of Bishkek with subsequent modeling and observation of the course of the wound process); III series — experimental, after a 30-day stay at the experimental high-mountain base of KSMA named after. A. A. Raimzhanov as part of the Central Scientific Research Laboratory (moved to the conditions of Bishkek with subsequent modeling and observation of the course of the wound process). Analysis of the data obtained shows that during aseptic inflammation, the opposite dynamics of the production of some of the key interleukins was observed, with IL-1β with a maximum concentration 12 hours from the onset of aseptic inflammation. The identified changes in the content of cytokines during experimental aseptic inflammation determined the timely change and adequate duration of cellular reactions. During purulent inflammation, the dynamics of cytokine secretion was unidirectional, which led to a delay in time of all cellular phases of the inflammatory process. During the period of deadaptation after a long stay in high altitude conditions, low secretion of IL-1 and IL-10 is observed, associated with the depletion of protective and adaptive mechanisms.

Analysis of Altitude and Ambient Temperature Effects on the Reactivity of Oxidation Catalysts in the Presence of H2

Worldwide emission standards are now required to cover engine operation under extreme ambient conditions, which affect the raw emissions and the efficiency of the exhaust aftertreatment systems. These regulations also target new combustion technologies for decarbonization, such as neat hydrogen (H2) combustion or dual-fuel strategies, which involve a challenge to the analysis of exhaust aftertreatment system requirements and performance. This work addresses the impact of high altitude and low ambient temperature conditions on the reactivity of an oxidation catalyst in the presence of H2. A reaction mechanism is proposed to cover the main conversion paths of CO, HC, and H2, including the formation and consumption of high-energy surface reaction intermediates. The mechanism has been implemented into a faster-than-real-time reduced-order model for multi-layer washcoat honeycomb catalytic converters. The model was utilized to investigate the effect of H2 concentration on the reactivity of CO and HC within the catalyst under various operating and ambient conditions. By applying the model and examining the selectivity towards different reaction pathways in the presence of H2, insights into surface intermediates and reactivity across different cross-sections of the monolith were obtained. This analysis discusses the underlying causes of reactivity changes promoted by H2 and its relative importance as a function of driving boundary conditions.