The estimation of the maximum displacement of steel beams with hexagonal web openings under impact loads is crucial for the anti-impact design of structures. The dynamic characteristics are complex, and the maximum displacement can be obtained experimentally, although the time cost, and expense of such experiments are high. In this context, a 6-5-1 artificial neural network model based on the Levenberg-Marquardt backpropagation algorithm was developed, incorporating 5-fold cross-validation techniques. Due to limited experimental data, 324 high-precision finite element models were created in bulk using Python-based ABAQUS software scripts to provide additional numerical data, and 26 specimens were designed for drop hammer tests to validate the finite element models. The artificial neural network model on the test data yielded a mean squared error of 0.0268, a mean absolute error of 0.0014, and a coefficient of determination value of 0.9806, with samples having an error of less than 10% comprising 77.16% of the total samples. Using Garson’s algorithm, the contribution of input features was estimated, and a full parameter analysis of the proposed model was conducted. The results indicate that the most significant factor is the impact mass, which accounts for 37.58%. When the impact mass increased from 530 kg to 630 kg, the maximum displacement of Q235 surged by 93.75%. The factor with the least impact is the opening spacing, accounting for 2%, with an average increase in maximum displacement of only 3.45%. Finally, ANN-based equations that can be used as design tools are established.