Heterogeneous Strain Research Articles

Anisotropy in tensile properties of a high strength metastable β titanium alloy

High strength metastable β titanium alloys are widely employed in the aircraft industry due to their outstanding strength-to-weight ratio. While components can endure complex in-service mechanical loading, the anisotropy in tensile properties has been the subject of limited attention. In this study, its origin was investigated focusing on the role played by millimeter scale β grains as they were recently identified as a source of heterogeneous deformation. Tensile properties of Ti-10V-2Fe-3Al processed via different thermomechanical routes were assessed using multiple sampling directions. In particular, elongation values were observed to vary significantly depending on the testing direction. A combination of SEM, EBSD, µ-CT and in-situ DIC during tensile tests was employed to clarify the underlying causes of this behavior. Substantial differences in strain heterogeneity and localization were found related to features of β grains, including their crystallographic and morphologic orientations. Furthermore, multiple fracture mechanisms were observed to derive from the differences in deformation behavior, and eventually compete to trigger specimen failure. Elongation values are then determined by both the degree of strain heterogeneity and the operating fracture mechanisms. These findings provide a new understanding of the role of the microstructure in the tensile behavior of high strength metastable β titanium alloys.

Local Strain-Dependent Etching Patterns of Chemical Vapor Deposited Molybdenum Disulfide.

This study reveals a local strain-dependent etching behavior that enables the formation of distinguished etching patterns in differently strained chemical vapor deposited (CVD) 2D molybdenum disulfide (MoS2) monolayers. It is demonstrated that when the local tensile strain of CVD 2D MoS2 is as uniformly low as ɛ ≈ 0.33% or less, the oxidative etching pattern possesses conventional triangular etching pits (TEPs), while when the local tensile strain is as uniformly high as ɛ ≈ 0.55% or larger, the oxidative etching pattern consist of uniformly oriented hexagonal etching channels (HECs). More interestingly, when the CVD 2D MoS2 monolayer has heterogenous strain distribution from ɛ ≈ 0.55% (center region) to ɛ ≈ 0.33% (perimeter region), the oxidative etching pattern comprise of non-uniformly hexagonal-mixed-parallel etching channels (HPECs). The further characterization and analysis reveal the formation mechanism of such strain-dependent etching patterns is built on the local strain-related fractures propagation under oxidative etching, as well as the anisotropy fractures-based oxidative etching kinetics. This study may enhance the understanding of the relationship between etching and growth features of 2D TMDs, and paves the way to etching-nanostructured (or defect) engineering of 2D TMDs and other 2D materials for potential applications in electrocatalysis and optoelectronics.

Whole-genome sequencing of Streptococcus uberis isolated from cows with mastitis in Thuringia.

Introduction. Streptococcus uberis is a common cause of mastitis in cattle, leading to significant economic losses. The widespread use of antimicrobials has contributed to the emergence of resistance, which poses a severe challenge in controlling S. uberis infection.Aim. The objective of this study was to gain insights into the antimicrobial resistance (AMR) and epidemiological typing of S. uberis isolated from milk collected from bovine mastitis on dairy farms in Thuringia.Methodology. In this study, 84 S. uberis isolates were obtained from cattle with clinical mastitis in Thuringia, their phenotypic and genotypic AMR were analyzed and their phylogenetic relationship was explored using whole-genome sequencing.Results. Genetically heterogeneous strains were found on the farms, but clusters of highly similar strains also circulated within the same farms. All isolates were sensitive to ampicillin, penicillin, ceftiofur, and vancomycin. However, 42.9%, 42.9%, 22.6%, 19.0%, and 13.0% were resistant to tetracycline, doxycycline, clindamycin, pirlimycin, and erythromycin, respectively. Thirty-nine strains were phenotypically resistant to two or more tested antibiotics. We identified a plasmid associated with macrolide and lincosamide resistance in 12% of the strains.Conclusion. The emergence of S. uberis strains resistant to multiple antibiotics highlights the importance of S. uberis surveillance and the prudent use of antimicrobials.

Giant transposons promote strain heterogeneity in a major fungal pathogen.

No "one size fits all" option exists for treating fungal infections in large part due to genetic and phenotypic variation among strains. Accounting for strain heterogeneity is thus fundamental for developing efficacious treatments and strategies for safeguarding human health. Here, we report significant progress towards achieving this goal by uncovering a previously hidden mechanism generating heterogeneity in the major human fungal pathogen Aspergillus fumigatus : giant transposons called Starships that span dozens of kilobases and mobilize fungal genes as cargo. By conducting the first systematic investigation of these unusual transposons in a single fungal species, we demonstrate their contributions to population-level variation at the genome, pangenome and transcriptome levels. The Starship atlas we developed will not only help account for variation introduced by these elements in laboratory experiments but will serve as a foundational resource for determining how Starships shape clinically-relevant phenotypes, such as antifungal resistance and pathogenicity.

3D strain heterogeneity and fracture studied by X-ray tomography and crystal plasticity in an aluminium alloy

Strong correlations between measured strain fields and crystal plasticity finite element (CP-FE) predictions based on the real microstructure are found for a plane strain tensile specimen made of 6016 T4 aluminium alloy. This is achieved using multimodal X-ray lab tomography giving access to both the initial grain structure and the strain evolution. The real microstructure of the central region of interest (ROI) of the undeformed specimen is obtained non destructively using lab-based diffraction contrast tomography (DCT) and meshing. An in situ tensile test, using absorption contrast tomography (ACT) is then performed for twelve loading increments up to fracture. Taking advantage of the plane strain condition, the evolution of the internal strain field is measured by two-dimensional digital image correlation (DIC) in the material bulk using the natural speckle provided by intermetallic particles. Early strain heterogeneities in the form of slanted bands, that are spatially stable over time, are revealed and the fracture path – determined from the post mortem scan – is found to coincide with the bands exhibiting maximum strain. CP-FE simulations are performed on the meshed microstructure of the specimen acquired by DCT and are compared with image correlation measurements. The measured strain fields are well described by 3D CP-FE predictions, whilst it is shown that neither a macroscopic anisotropic plasticity model nor a CP-FE simulation with random grain orientations could reproduce the measurements.

Low-cycle fatigue properties and fracture location transition mechanism of dissimilar steel welded joints in towers of wind turbines

This study investigates the low-cycle fatigue (LCF) behavior of two types of dissimilar steel welded joints (DSWJs): AISI 1035 & S550Q and AISI 1020 & S550Q. Digital image correlation (DIC) and finite element analysis (FEA) were used to analyze the heterogeneous strain distribution. The results reveal that the location of maximum strain, which is load-dependent and related to strength mismatch, corresponds to the fracture location of the LCF specimens. Specifically, the crack initiation site shifts from the weld toe to the base metal as strain increases. Moreover, the weld reinforcement of the DSWJs significantly affects the failure site transition, introducing strain concentration at lower stress levels while enhancing deformation resistance at higher stress levels, as evidenced by DIC strain measurements and FEA. These findings highlight the importance of joint reinforcement profile, strength mismatch and external load level in designing DSWJs for wind turbine towers against LCF failure.

Deep CO2 emissions facilitated by localized shear deformation: A case study of the Karakoram fault system, western Tibet

Strike-slip faults play a significant role in creating deeply penetrating fractures with high permeability, thus promoting rapid migration of CO2-rich fluids to the surface. However, there are rare observations regarding how strike-slip movement could affect deep CO2 emissions. Here, we focus on the Karakoram fault system (KKFS), western Tibet, to estimate diffuse soil CO2 fluxes and to unravel potential controlling factors for CO2 emissions. Average CO2 fluxes of geothermal fields range in 22–2475 g m−2 d−1, significantly higher than the across-fault profiles (6–116 g m−2 d−1). A mass balance model based on δ13C-CO2 and CO2 concentration of soil gases reveals that deep carbon constitutes 49.1–91.5 % (average = 73.9 %) and 0.2–40.5 % (average = 25.5 %) of soil-gas carbon released from geothermal fields and across-fault profiles, respectively. Deep carbon could be produced by thermal decomposition of crustal rocks considering CO2-rich fluids with radiogenic helium isotopes. Strikingly, higher CO2 fluxes preferentially occur in geothermal fields along a bending segment of the KKFS, where localized shear deformation is prominent as documented by high slip rates over geological timescales, dense splay faults, clustering of earthquake events, and elevated strain rates. We suggest that high stress acting on the KKFS bend could enhance the deformation and fracturing of fault zone rocks, leading to production of metamorphic CO2 and efficient release of CO2-rich fluids through the highly permeable fault system. Our results could shed new light on CO2 origins and fluxes of strike-slip faults that are characterized by spatially heterogeneous strain partitioning and thus localized enhanced shear deformation.

Probing the Linear-to-Plastic Transition in Polymer Nanocomposites via Atomistic Simulations: The Role of Interphases.

Polymer nanocomposites have found ubiquitous use across diverse industries, attributable to their distinctive properties and enhanced mechanical performance compared to conventional materials. Elucidating the elastic-to-plastic transition in polymer nanocomposites under diverse mechanical loads is paramount for the bespoke design of materials with desired mechanical attributes. In the current work, the elastic-to-plastic transition is probed in model systems of polyethylene oxide (PEO) and silica, SiO2, nanoparticles, through detailed atomistic molecular dynamics simulations. This comprehensive, multi-scale analysis unveils pivotal markers of the elastic-to-plastic transition, highlighting the quintessential role of microstructural and regional heterogeneities in density, strain, and stress fields, featuring the polymer-nanoparticle interphase region. At the atomic level, the behavior of polymer chains interacting with nanoparticle surfaces is traced, differentiating between free and adsorbed chains, and identifying the microscopic origins of the linear-to-plastic transition. The mechanical behavior of subregions are characterized within the PEO/SiO2 nanocomposites, focusing on the interphase and bulk-like polymer areas, probing stress heterogeneities and their decomposition into various force contributions. At the inception of plasticity, a disruption is discerned in isotropy of the polymeric density field, the emergence of low-density regions, and microscopic voids/cavities within the polymer matrix concomitant with a transition of adsorbed chains to free. The yield strain also emerges as an inflection point in the local versus global strain diagram, demarcating the elastic limit, and the plastic regime shows pronounced strain heterogeneities. The decomposition of the atomic Virial stress into bonded and non-bonded interactions indicates that the rigidity of the material is primarily governed by non-bonded interactions, significantly influenced by the volume fraction of the nanoparticle. These findings emphasize the importance of the microstructural and micromechanical environment at the polymer-nanoparticle interface on the linear-to-plastic transition, which is of great importance in the design of nanocomposite materials with advanced mechanical properties.

Opportunistic Features of Non-Clostridium botulinum Strains Containing bont Gene Cluster.

The cluster of genes determining the production of botulinum toxins is an attribute of not only the Clostridium botulinum species. This cluster is also found in other members of the Clostridium genus, such as C. baratii, C. butyricum, and C. sporogenes. The occurrence of a botulinum-like cluster has also been recorded in strains of other genera, i.e., Enterococcus faecium, as well as in a Gram-negative species isolated from freshwater sediments; however, the biological activity of bont-related genes has not been noted. It can be said that the mentioned species have a dual nature. Another species with a dual nature is C. butyricum. This bacterium is a common human and animal gut commensal bacterium and is also frequently found in the environment. Although non-toxigenic strains are currently used as probiotics in Asia, other strains have been implicated in pathological conditions, such as botulism in infants or necrotizing enterocolitis in preterm neonates. Additionally, C. baratii strains are rare opportunistic pathogens associated with botulism intoxication. They have been isolated from food and soil and can be carried asymptomatically or cause botulism outbreaks in animals and humans. In addition to the mentioned clostridia, the other microorganisms considered as non-toxigenic have also been suspected of carrying botulinum cluster Gram-negative bacteria, such as Chryseobacterium piperi isolated from freshwater sediments; however, the biological activity of bont-related genes has not been noted. Additionally, Enterococcus faecium strains have been discovered carrying BoNT-related clusters (BoNT/En). Literature data regarding the heterogeneity of BoNT-producing strains indicate the requirement to reclassify C. botulinum species and other microorganisms able to produce BoNTs or possess botulinum-like gene clusters. This article aims to show the dual nature of Clostridium strains not belonging to the C. botulinum species that are sporadically able to carry bont clusters, which are usually considered saprophytic and even probiotic, and bont-like clusters in microorganisms from other genera. The aim was also to consider the genetic mechanisms of botulinum cluster expression in strains that are considered opportunistic and the microbiological safety aspects associated with their occurrence in the environment.

Strain heterogeneity in a non-pathogenic Aspergillus fungus highlights factors associated with virulence

Fungal pathogens exhibit extensive strain heterogeneity, including variation in virulence. Whether closely related non-pathogenic species also exhibit strain heterogeneity remains unknown. Here, we comprehensively characterized the pathogenic potentials (i.e., the ability to cause morbidity and mortality) of 16 diverse strains of Aspergillus fischeri, a non-pathogenic close relative of the major pathogen Aspergillus fumigatus. In vitro immune response assays and in vivo virulence assays using a mouse model of pulmonary aspergillosis showed that A. fischeri strains varied widely in their pathogenic potential. Furthermore, pangenome analyses suggest that A. fischeri genomic and phenotypic diversity is even greater. Genomic, transcriptomic, and metabolic profiling identified several pathways and secondary metabolites associated with variation in virulence. Notably, strain virulence was associated with the simultaneous presence of the secondary metabolites hexadehydroastechrome and gliotoxin. We submit that examining the pathogenic potentials of non-pathogenic close relatives is key for understanding the origins of fungal pathogenicity.

Mapping Spatial Strain Distribution and Its Effects on Optoelectronic Properties in Wrinkled Perovskite Films.

Organic-inorganic halide perovskite films, fabricated by using the antisolvent method, have garnered intense attention for their application in high-efficiency and stable solar cells. These films characteristically develop periodic wrinkled microstructures. Previous research has indicated that macroscopic residual strain significantly influences the optoelectronic behaviors of these films. However, the detailed interplay between the wrinkled morphology, strain distribution, and local photophysical properties at the micro- and nanoscale has not been fully elucidated. Here, we explore the microscopic morphology-strain-property relationship within wrinkled perovskite films employing correlative micro-optical and nanoelectrical microscopy techniques. Microphotoluminescence (PL) mapping supplemented by in situ strain PL measurements identifies a heterogeneous spatial strain distribution across the microstructural hills and valleys. Additionally, light-intensity-dependent photoconductive atomic force microscopy reveals that valleys experiencing less compressive strain exhibit a lower conductivity and a higher propensity for ion migration. The findings underscore the potential of targeted strain engineering to optimize the performance and longevity of perovskite solar cells.

Study on the grain development and dislocation evolution from dynamic to post-dynamic stage in IN718Plus superalloy; the role of second-phase

The introduction of hexagonal η-Ni3(Al,Ti)0.5Nb0.5 phase can refine and homogenize the microstructure of IN718Plus superalloy during hot working, but produces more complicated recrystallization behavior associated with second-phase modified structural mechanisms. Derived from this perspective, the role of η phase on recrystallization mechanisms and grain development from dynamic deformation to post-dynamic dwell was systematically investigated. The results indicated that η phase delayed the throughout recrystallization procedure and grain development. During dynamic deformation, boundary η inhibited discontinuous dynamic recrystallization via preventing the bulging of original grain boundaries, while intragranular lamellar η promoted heterogeneous strain concentration, stimulated random dislocations for continuous dynamic recrystallization and dislocation walls for banded dislocation substructures, which limited recrystallization occurrence in dynamic stage. During post-dynamic dwell, boundary η dissolution increased the nucleation sites for discontinuous static recrystallization, and η phase restricted grain boundary migration to provide abundant nucleation sites for continuous static recrystallization (CSRX). The banded dislocation substructures slowly evolved into subgrains to further form banded CSRX grains, which reduced grain size during early post-dynamic stage. After fully recrystallization, the residual η protected the small grains and impeded grain growth, meanwhile controlled the grain distribution. As a result, η phase induced sluggish dislocation evolution and recrystallization nucleation, consequently suppressed grain development and obtained the more homogeneous and fine grain configuration.

Influence of surface pre-deformation on the Portevin-Le Chatelier effect and the related multiscale complexity of plastic flow in an Al-Mg alloy

The influence of the surface pre-deformation on jerky flow caused by the Portevin-Le Chatelier (PLC) effect was investigated using flat tensile specimens of an Al-Mg alloy. Although jerky flow represents a macroscopic plastic instability, the underlying mechanisms stem from self-organization of dislocations, which pertains to deformation processes at mesoscopic scales. To provide a comprehensive approach, the investigation was carried out by coupling tensile tests, digital image correlation and acoustic emission techniques, each targeting a particular range of scales. Thin superficial layers were pre-deformed using surface mechanical attrition technique (SMAT). It was found that the observed effects depend on which surfaces are processed. Overall, the treated samples exhibited an enhanced yield strength without deterioration of ductility in comparison with the initial material. These changes in the general mechanical behavior are likely to be correlated with the changes in jerky flow. Using digital image correlation, a tendency to delocalization of bursts of plastic flow was found for both the PLC bands and smaller-scale strain heterogeneities outside the bands, the latter having been little studied so far. Unexpectedly, SMAT occurred to modify plastic flow drastically at this scale. In addition to clarification of the role played by the surface in the PLC effect, these findings provide new insights into relationships between deformation processes at distinct scales.

Mechanical ventilation guided by driving pressure optimizes local pulmonary biomechanics in an ovine model.

Mechanical ventilation exposes the lung to injurious stresses and strains that can negatively affect clinical outcomes in acute respiratory distress syndrome or cause pulmonary complications after general anesthesia. Excess global lung strain, estimated as increased respiratory system driving pressure, is associated with mortality related to mechanical ventilation. The role of small-dimension biomechanical factors underlying this association and their spatial heterogeneity within the lung are currently unknown. Using four-dimensional computed tomography with a voxel resolution of 2.4 cubic millimeters and a multiresolution convolutional neural network for whole-lung image segmentation, we dynamically measured voxel-wise lung inflation and tidal parenchymal strains. Healthy or injured ovine lungs were evaluated as the mechanical ventilation positive end-expiratory pressure (PEEP) was titrated from 20 to 2 centimeters of water. The PEEP of minimal driving pressure (PEEPDP) optimized local lung biomechanics. We observed a greater rate of change in nonaerated lung mass with respect to PEEP below PEEPDP compared with PEEP values above this threshold. PEEPDP similarly characterized a breaking point in the relationships between PEEP and SD of local tidal parenchymal strain, the 95th percentile of local strains, and the magnitude of tidal overdistension. These findings advance the understanding of lung collapse, tidal overdistension, and strain heterogeneity as local triggers of ventilator-induced lung injury in large-animal lungs similar to those of humans and could inform the clinical management of mechanical ventilation to improve local lung biomechanics.

Multiscale homogenization of thermo-mechanical viscoelastic response of 3D orthogonal composites with time-dependent CTEs

Viscoelastic behavior of the matrix affects the thermomechanical response of polymer composites. This study investigated the structural effects of the time-dependent thermal expansion response of 3D orthogonal woven composite (3DOWC) by combining experiments and finite element modeling (FEM), incorporating the viscoelasticity of its constituents. The deformation characteristics at three orthogonal cross-sections, in the presence of structure-related strain fields at various temperatures, were investigated using digital image correlation method. Findings reveal heterogeneity and localization of the thermal strain, characterized by periodic stripes with alternating high strain regions on the resin and low strain regions on the yarn. This localization intensifies at higher temperatures, but diminishes with increased distance from the interface. Furthermore, the composites exhibit time-dependent thermal expansion behavior derived from the time-independent thermal expansion of its constituents, which cannot be captured in classic elastic material model. FEM reveals that thermal stresses are concentrated at the interfaces between constituents with significant differences in their coefficients of thermal expansion (CTE), particularly at the interface between the resins and axial yarns. The maximum thermal stress occurs in the binder yarns over the considered temperature range due to their low volume content, which should be considered in practical applications.

Detection of pathogenic, heteropathogenic and hybrid Escherichia coli strains in psittacines from zoos and breeders in the state of Ceará, Brazil

The current study aimed to detect virulence, hetero-pathogenicity, and hybridization genes in Escherichia coli strains, previously isolated from cloacal swabs in commercial breeding psittacines and zoological collections, via multiplex PCR. A total of 68 strains of E. coli, previously isolated from psittacines in zoos and commercial breeding facilities in Ceará, Brazil, were assessed for the presence of the following genes and/or probes: eae, bfpA (EPEC - Enteropathogenic E. coli), CVD432 (EAEC - Enteroaggregative E. coli); LT gene and ST gene (ETEC - Enterotoxigenic E. coli); ipaH (EIEC - Enteroinvasive E. coli); stx1 and stx2 (STEC - Shiga toxin-producing E. coli); iroN, ompT, hlyF, iss, and iutA (APEC - Avian pathogenic E. coli). Of the 68 E. coli strains analyzed, 61 (98.7 %) were positive for the following genes and/or probes: Stx1 (61/98.7 %), ST gene (54/79.4 %), CVD432 (49/72 %), bfpA (44/64.7 %), eae (42/61.8 %), Stx2 (41/60.3 %), ipaH (34/50 %), LT gene (33/48.5 %), iroN (21/30.9 %), hlyF (11/6.2 %), iss (06/8.8 %) and iutA (06/8.8 %). The following diarrheagenic pathotypes were identified: 66 (97 %) from STEC, 49 (72 %) from EAEC, 35 (52 %) from EIEC, 25 (37 %) from ETEC, and one (1.5 %) from EPEC. Regarding hetero-pathogenicity, 50 (74 %) heterogeneous strains were identified. Positivity for APEC was seen in four (6 %) strains, all characterized as pathogenic hybrids. This study describes significant associations of virulence factors in E. coli strains DEC/DEC and DEC/APEC, which were isolated from psittacines and may be potentially harmful to One Health.

Xanthomonas Strains Isolated from Hosts in the Family Araceae Reveal Diverse Phylogenetic Relationships and Origins.

The family Araceae, comprising ornamentals including Anthurium, Dieffenbachia, Philodendron, Colocasia, and Zantedeschia, is susceptible to Xanthomonas pathogens. Previous analyses have established heterogeneity in aroid strains, yet unresolved taxonomic positions and dynamics between Xanthomonas and frequently associated Stenotrophomonas in aroids necessitate in-depth genetic investigation to resolve these complex relationships. This study utilized multilocus sequence analysis of housekeeping genes atpD, dnaA, dnaK, gltA, and gyrB to investigate 59 aroid strains, selected based on hosts, time, and geographical origins. After adding sequences from additional strains from NCBI GenBank, analysis of 161 concatenated sequences indicated that all aroid strains fell within Xanthomonas and Stenotrophomonas. Thirty-six strains isolated from Anthurium grouped under X. phaseoli, with outliers including one strain each in X. arboricola and X. sacchari and two in Stenotrophomonas. Six strains from Caladium, Dieffenbachia, and Philodendron formed host-specific subgroups within X. euvesicatoria. One strain from Dieffenbachia aligned with X. campestris, whereas strains from Colocasia, Aglaonema, and Spathiphyllum clustered with X. sacchari. Apart from the zantedeschia strain described as X. arboricola pv. zantedeschiae, two colocasia, one epipremnum, and one anthurium strain joined the X. arboricola group. Overall, this study revealed significant heterogeneity among aroid strains, with anthurium strains clustering closely despite distant geographical origins. The analysis underscores the complexity of host-pathogen specificity within Xanthomonas and emphasizes the need for further taxonomic clarification through whole-genome analysis of representative strains. The findings of this research will facilitate strain selection for inclusivity and exclusivity panels in developing diagnostic assays for X. phaseoli and xanthomonads affecting aroids.

Investigating the Hepatitis E Virus (HEV) Diversity in Rat Reservoirs from Northern Italy.

Hepatitis E virus belonging to the Rocahepevirus ratti species, genotype HEV-C1, has been extensively reported in rats in Europe, Asia and North America. Recently, human cases of hepatitis associated with HEV-C1 infection have been reported, but the zoonotic nature of rat-HEV remains controversial. The transmission route of rat-HEV is unidentified and requires further investigation. The HEV strains of the Paslahepevirus balayani species, belonging to the same Hepeviridae family, and including the zoonotic genotype HEV-3 usually found in pigs, have also sporadically been identified in rats. We sampled 115 rats (liver, lung, feces) between 2020 and 2023 in Northeast Italy and the HEV detection was carried out by using Reverse Transcription PCR. HEV RNA was detected in 3/115 (2.6%) rats who tested positive for HEV-C1 strains in paired lung, intestinal contents and liver samples. Overall, none tested positive for the Paslahepevirus balayani strains. In conclusion, our results confirm the presence of HEV-rat in Italy with a prevalence similar to previous studies but show that there is a wide heterogeneity of strains in circulation. The detection of HEV-C1 genotype of Rocahepevirus ratti species in some human cases of acute hepatitis suggests that HEV-C1 may be an underestimated source of human infections. This finding, with the geographically widespread detection of HEV-C1 in rats, raises questions about the role of rats as hosts for both HEV-C1 and HEV-3 and the possibility of zoonotic transmission.

Influence of corrugated/flat rolling on microstructure and tensile property of coarse-grained AZ31–0.25Ca cast alloy

Novel corrugated rolling technology (CR) and conventional flat rolling (FR) were employed for rolling the AZ31–0.25Ca cast alloy without prior predeformation. The impact of corrugated rolling and/or flat rolling on texture modification, microstructure evolution, and mechanical properties of the magnesium alloy during two-pass rolling was examined using optical microscopy (OM), X-ray diffraction (XRD), scanning electron microscopy (SEM), electron backscatter diffraction (EBSD), transmission electron microscopy (TEM), tensile tests at ambient temperature, and ABAQUS finite element simulation analysis. The results indicate that the AZ31–0.25Ca cast alloy, after homogenization with a coarse grain structure of ∼200 μm, can endure significant plastic deformation with a 60 % total rolling reduction in both corrugated-flat rolling (CFR) and flat-flat rolling (FFR) without experiencing edge cracking. Twinning is activated dramatically except for dislocation slip during CFR, which induced high-density dislocations piling up at the twin boundaries and grain boundaries of α-Mg grains. The CFR sheet exhibited a macroheterogeneous structure attributed to the distinctive corrugated roll profile. Elevated shear strain and intensified frictional shear stress were observed at the trough, resulting in the formation of an asymmetric cross shear band in the CFR sheet. Denser deformation twins and finer recrystallized grains are present at the trough, whereas fewer and coarser ones are observed at the peak, while there is a significant texture weakening effect for corrugated rolling in contrast to the conventional flat rolling. but the stress and strain heterogeneity and asymmetric cross shear band also induce strong stress concentration and result in a premature cleavage fracture and a lower tensile property in CFR sheet compared to that of the FFR sheet.

Data-Driven Identification unravels multiaxial mechanical response of a carbon-black filled elastomer during ageing

Under environmental exposure, the mechanical properties of elastomers change due to ageing, all while enduring mechanical service loading conditions. The influence of ageing on the multiaxial mechanical response of elastomers remains an understudied question, lacking exploration in both experimental evidence and modelling proposals. The present study describes an experimental/numerical approach to characterize the multiaxial behaviour of elastomers with consideration of ageing. This technique associates complex experimental tests conducted with a hexapod device, with a Data-Driven Identification (DDI) algorithm. Practically, heterogeneous strain fields are measured by Digital Image Correlation (DIC), and the corresponding stress and energy fields are calculated by DDI. These fields are visualized through three-dimensional maps, encompassing kinematical quantities and strain energy density. These maps convincingly capture the stiffening induced by ageing, in different deformation modes. Finally, the coupling between ageing and multiaxiality is foregathered in a material database that can be fitted for further modelling purposes.