Herpetofaunal Diversity Research Articles

Three Continents Claiming an Archipelago: The Evolution of Aegean’s Herpetofaunal Diversity

The area of the Aegean can be described as one of nature’s most active laboratories. The contemporary geomorphology of the Aegean is a result of diverse and still ongoing geological events, which coupled with climate changes, have created mountains and thousands of islands. The Aegean bridges three continents, where human activity has been recorded for at least 10,000 years. Herpetofauna diversity offered early researchers the possibility of describing patterns in the Aegean, especially as the distributional limit for several species and faunal elements. The patterns initially described at a rather coarse scale formed the frame on which the application of new techniques opened new views and permitted finer analyses. Here, we assess recent works on the Aegean’s herpetofauna, outlining the role of sea barriers, especially the Mid Aegean Trench (MAT). We propose four basic patterns (pre-MAT, post-MAT, newcomers, and that of an outlier) and discuss exceptions to these patterns, to interpret the diversity recorded. The interdisciplinary study of taxonomy helps explaining the observed diversity and provides powerful arguments for how exploring diversity can be used to explain more than biological processes.

Reptile and Amphibian Responses to Restoration of Fire‐Maintained Pine Woodlands

Abstract Fire‐maintained woodlands and savannas are important ecosystems for vertebrates in many regions of the world. These ecosystems are being restored by forest managers, but little information exists on herpetofaunal responses to this restoration in areas dominated by shortleaf pine (Pinus echinata). We compared habitat characteristics and herpetofaunal communities in restored pine woodlands to relatively unmanaged, second‐growth forests in the Ouachita Mountains of western Arkansas, USA. We found woodland restoration with periodic burning affected species differently; some species benefited, some species appeared negatively affected, but most species did not respond clearly either way. Overall reptile captures were significantly (p = 0.041) greater in pine‐woodlands than in unrestored forest; one species of snake and three species of lizards were captured more often in woodlands than unrestored forests. Among anurans, we found no significant difference in captures between woodlands and unrestored forests for any species. Among salamanders, we captured western slimy salamanders (Plethodon albagula) almost exclusively in unrestored forest, but captures of other species did not differ between the two treatments. Historically, the Ouachita region likely consisted of a mosaic that included both fire‐maintained habitats (woodlands, savannas, and prairies) and areas of denser forest on mesic sites that were less likely to burn. Consequently, landscapes that retain both open woodlands and denser, less‐intensely burned forest (in the form of unharvested greenbelts or separate stands) would likely promote and maintain a greater diversity of herpetofauna.

Amphibians and Reptiles of Dinghushan in Guangdong Province, China’s Oldest Nature Reserve

Dinghushan is the only nature reserve at the Tropic of Cancer in mainland China with extensive old growth monsoonal hardwood forest. We here assemble a comprehensive list, with life history notes, of amphibians and reptiles of Dinghushan. Relevant records were scattered over three centuries since the first in 1886. A total of 71 species are known to occur in the reserve, and this number of species per area far exceeds that predicted by classic island biogeographic theory. Among these 71 species, nine are new records: Five frogs (Amolops ricketti, Hylarana macrodactyla, Hylarana taipehensis, Odorrana schmackeri, and Occidozyga lima) and four snakes (Oligodon cinereus, Sinonatrix percarinata, Sinomicrurus macclellandi, and Trimeresurus stejnegeri). One undetermined skink may confound the generic definitions for the genera of Scincella and Sphenomorphus. Among these 71 species, 21 that are not closely associated with old growth forest have not been seen recently. Forest recovery and reforestation of farmland over five decades since the nature reserve was established in 1956 may have contributed to the decline of these species. Dinghushan has 97% of its species occurring strictly in the Oriental zone; among them, about 78% (55) occur both in the South China and Central China regions, 21% (15) occur only in the South China region, and one species was previously known only from the Central China region. The unique location of Dinghushan makes long-term monitoring of its herpetofaunal diversity important for future collaborative studies on a global scale.

The herpetofauna of Sonora, Mexico, with comparisons to adjoining states

Situated in the topographically complex transition between the Neotropics and the temperate biomes of North America, the state of Sonora, Mexico, has an extraordinarily diverse herpetofauna. Surprisingly little research has been conducted on the state’s amphibians and reptiles and many systematic and biogeographic questions remain unanswered. To facilitate future research, we provide a checklist of Sonora’s herpetofauna, documenting species presence based on museum specimens, our fieldwork, and published research. Sonora’s herpetofauna is placed in a regional biogeographic perspective via a checklist for the six adjoining states together with faunal analyses. A total of 402 species of amphibians and reptiles are recorded from these seven states. Sonora has the greatest species richness (187 species), followed by Chihuahua (169 species), and Sinaloa (146 species). Sonora's herpetofauna is most similar to that of Chihuahua, with which it shares a long border. Eleven biogeographic affinity-based faunal groups are recognized. Of these, three are dominant in Sonora: a core group classified as "Sonoran" demonstrates strong affinity to Sonoran Desertscrub and Sinaloan Thornscrub communities; a Tropical group - with many species reaching their northern distributional limits in the state; and a Madrean group consisting largely of montane species. Our state-level faunal analysis provides some evidence of peninsular depauperization of the herpetofauna on the Baja California peninsula due in part to the small number of Neotropical species present in Baja California Sur. Our faunal analysis points toward distinctive mainland and peninsular Sonoran Desert herpetofaunas centered on Sonora and the Baja California Peninsula, respectively, each with about 50 non-insular species, and each with species-level endemism nearing 50%.

Land‐cover data improve bioclimatic models for anurans and turtles at a regional scale

AbstractAim We investigated whether accounting for land cover could improve bioclimatic models for eight species of anurans and three species of turtles at a regional scale. We then tested whether accounting for spatial autocorrelation could significantly improve bioclimatic models after statistically controlling for the effects of land cover.Location Nova Scotia, eastern Canada.Methods Species distribution data were taken from a recent (1999–2003) herpetofaunal atlas. Generalized linear models were used to relate the presence or absence of each species to climate and land‐cover variables at a 10‐km resolution. We then accounted for spatial autocorrelation using an autocovariate or third‐order trend surface of the geographical coordinates of each grid square. Finally, variance partitioning was used to explore the independent and joint contributions of climate, land cover and spatial autocorrelation.Results The inclusion of land cover significantly increased the explanatory power of bioclimatic models for 10 of the 11 species. Furthermore, including land cover significantly increased predictive performance for eight of the 11 species. Accounting for spatial autocorrelation improved model fit for rare species but generally did not improve prediction success. Variance partitioning demonstrated that this lack of improvement was a result of the high correlation between climate and trend‐surface variables.Main conclusions The results of this study suggest that accounting for the effects of land cover can significantly improve the explanatory and predictive power of bioclimatic models for anurans and turtles at a regional scale. We argue that the integration of climate and land‐cover data is likely to produce more accurate spatial predictions of contemporary herpetofaunal diversity. However, the use of land‐cover simulations in climate‐induced range‐shift projections introduces additional uncertainty into the predictions of bioclimatic models. Further research is therefore needed to determine whether accounting for the effects of land cover in range‐shift projections is merited.

Conservation value of cacao agroforestry for amphibians and reptiles in South‐East Asia: combining correlative models with follow‐up field experiments

Summary 1. Although agricultural expansion is a primary threat to tropical biodiversity, experimental studies evaluating the conservation value of tropical agricultural habitats are scarce. In particular, little is known about the sensitivity of amphibians and reptiles to habitat disturbance in areas of very high diversity such as South‐East Asia. 2. We used a two‐step approach to determine the relationship between habitat complexity and conservation value of cacao agroforestry for herpetological diversity in Sulawesi (Indonesia). Indonesia is the third largest cacao‐exporting country globally and forest conversion to cacao plantations is a major threat to its biodiversity. We first sampled 43 cacao plantations six times to determine the environmental variables that best explained herpetofaunal diversity patterns using a Bayesian model selection approach. Based on these results, we experimentally manipulated leaf litter thickness (LLT), number of branch piles (LOGS) and LLT + LOGS combinations in the cacao plots. The experimental data were analysed using Bayesian hierarchical regression. 3. The best supported correlative models incorporated LLT, LOGS, air temperature and the ratio between leaf litter and shrub cover, showing the importance of habitat heterogeneity and suggesting climate change sensitivity. The subsequent structural manipulation of these attributes changed amphibian and reptile species richness, and reptile abundance, but only addition of leaf litter did so in a biologically meaningful way, providing microhabitat resources. However, the main beneficiaries were common disturbance‐tolerant reptiles. 4. Synthesis and applications. The different results from the correlative model and the independent manipulative experiments showed how important such a combined approach is to derive adequate conservation management recommendations. Increasing leaf litter in cacao agroforestry will work best if implemented on a landscape scale to incorporate sufficient environmental variation and species life histories. This will mainly enhance the richness and abundance of disturbance‐tolerant species, which still may maintain ecosystem functions such as pest removal. Particularly for rare species, native forests remain critical for herpetological richness. The direct temperature sensitivity suggests that future climate change impacts may be severe for herpetological diversity in plantation habitats and, hence, demand further research.

Herpetofauna em uma área de Floresta Atlântica na Ilha Anchieta, município de Ubatuba, sudeste do Brasil

Este trabalho teve como objetivo o estudo da diversidade da herpetofauna do Parque Estadual da Ilha Anchieta, município de Ubatuba, localizada no litoral norte do estado de São Paulo, enfocando os seguintes aspectos: diversidade, distribuição e padrões de atividade sazonal. Entre julho de 2005 e junho de 2006 foram realizadas viagens mensais à ilha, totalizando 55 dias de trabalhos de campo. Para a amostragem foram utilizados os métodos busca ativa, armadilhas de interceptação e queda, armadilhas de funil e coleta por terceiros, Busca ativa e armadilhas de interceptação e queda apresentaram melhor desempenho, e os demais métodos se mostraram complementares. Foram inventariadas 17 espécies de anfíbios anuros distribuídas em oito famílias, e oito espécies de répteis das quais cinco são espécies de lagartos de quatro famílias e três de serpentes de duas famílias. Como observado na grande maioria das pesquisas em áreas tropicais, a área de estudo apresentou relativamente baixa equabilidade, porém, diferente de outras comunidades estudadas, apresentou um maior número de espécies comuns em relação às espécies raras. A riqueza de anfíbios anuros foi mais alta nas fisionomias consideradas fechadas (mata latifoliada densa e mata latifoliada rala) em relação às abertas, enquanto que a riqueza de répteis foi mais representativa no campo antrópico (fisionomia considerada aberta). A análise de correlação demonstrou que a riqueza de anfíbios não foi significativa em relação aos elementos climáticos, enquanto a riqueza de répteis foi significativamente correlacionada às médias de temperaturas máxima e mínima. A presença de inúmeras peculiaridades reforça a importância da conservação do Parque Estadual da Ilha Anchieta.

Herpetofauna dos remanescentes de Mata Atlântica da região de Tapiraí e Piedade, SP, sudeste do Brasil

Apresentamos aqui a lista de espécies de anfíbios e répteis registradas nos remanescentes de Mata Atlântica contínua e fragmentada da região de Tapiraí e Piedade, Planalto Atlântico de São Paulo, sudeste do Brasil. Amostramos 21 localidades na região, sendo 15 fragmentos florestais e seis localidades do interior e entorno do Parque Estadual do Jurupará, único remanescente legalmente protegido da região. Registramos 47 espécies de anfíbios anuros, uma espécie de gymnophiona, oito espécies de lagartos, uma espécie de anfisbena, 46 espécies de serpentes e uma espécie de quelônio. Esta lista conta com espécimes capturados pelos métodos de armadilhas de interceptação e queda, procura visual e auditiva, encontros ocasionais, capturas por terceiros e, no caso das serpentes, também por registros em coleção científica. A combinação entre métodos foi eficiente na amostragem da herpetofauna da região, evidenciando a necessidade de amostragem com métodos complementares. Os remanescentes de Tapiraí e Piedade apresentam espécies de anfíbios e répteis típicas de áreas de Mata Atlântica e alta riqueza de espécies, em relação a outros estudos realizados no planalto. A presença de espécies de distribuição restrita, ou presentes em listas de fauna ameaçada, reforçam a necessidade de conservação dos remanescentes de Mata Atlântica na região, que apesar de abrigar grande diversidade está ameaçada pelos efeitos da fragmentação florestal.

Effects of hurricanes Ivan, Katrina, and Rita on a southeastern Louisiana herpetofauna

Tropical weather disturbances are a major influence on coastal wetlands in North America. However, studies of their impact on biotic communities are rare. The abundance and species composition of amphibians and reptiles were investigated within levee, herbaceous marsh, and forested swamp habitats in southeastern Louisiana from 2002 to 2004 and again in 2005 to 2006. During the course of this study, three major hurricanes (Ivan, Katrina, and Rita) affected our study sites. This allowed us to opportunistically document the effect of major storm disturbances by comparing species richness, diversity, community assemblage, and abundance of amphibians and reptiles before and after hurricane events. We also used a previous study conducted in the same area during the late 1980's to assess long- term community composition changes. We documented the highest species richness in the forested swamp habitat (23 species), but the most diverse assemblage was found in marsh habitat (H 95 2.082). Overall, herpetofaunal diversity decreased and evenness increased in each habitat following hurricanes Ivan and Katrina/Rita. Drastic decreases in overall abundance of amphibians occurred, while the effect on reptile abundance varied with habitat. Reduced abundance of reptiles in marsh was recorded over the course of the study, while abundances in adjacent levee habitat increased, suggesting displacement of certain reptiles from the marsh to the levee. Significant saltwater intrusion was recorded in marsh and levee habitats, but not in the forested swamp. The hurricanes altered community composition and increased species evenness within each habitat, potentially affecting long-term community dynamics and species interactions.

A rapid assessment of herpetofaunal diversity in variously altered habitats on Dominica

In June 2008, we conducted a survey of Dominican herpetofaunal communities in habitats variously disturbed by human activity. Our rapid assessment found the highest abundance and species richness in moderately to substantially modified areas. We found relatively few species and low numbers of individuals in relatively natural high-elevation sites, dramatically altered urban areas, and active agricultural fields. In habitats subjected to intermediate levels of disturbance, such as a residential area and an inactive agricultural site invaded by dense exotic vegetation, complex physical habitat structure appeared to support the greatest herpetofaunal diversity. These results suggest that conservation efforts in the West Indies need not be restricted to pristine habitats, but also might include protection of slightly to moderately disturbed areas that may sustain greater biodiversity.

The herpetofauna of Faure Island, Shark Bay, Western Australia

The currently recorded Fame Island herpetofauna comprises 24 species of reptile. This is a strict subset of the more diverse herpetofauna recorded from adjacent Peron Peninsula. Wc describe the broader geographic distributions and habitat associations of each species, and discuss the biogeography of the island herpelofauna in the context of the Carnarvon Basin biogeographic region. The reduced island diversity is presumably due to a combination of lesser sampling effort and'drop out' extinctions following drowning of the continental shelf and severance of the island around 6S00 years ago.

Genetic diversity in the gecko Tarentola mauritanica within the Iberian Peninsula

AbstractThe Mediterranean gecko Tarentola mauritanica is one of the most abundant reptile species in the western Mediterranean Basin. We use mitochondrial DNA sequences, 12sRNA and 16sRNA, to analyse the patterns of distribution in the Iberian Peninsula. The results show three well defined lineages. The first one, already known to be widespread throughout Europe, is distributed along the eastern coast and southern areas of the Iberian Peninsula. The second one, endemic to the Iberian Peninsula, is widely found in central regions; and the third lineage, found so far only in two southern localities is unrelated to the other Iberian lineages, but rather to a Northern Moroccan clade. The genetic variability found within the Iberian haplogroup and the existence of a single haplotype within the European lineage suggests a complex pattern of rapid radiations and anthropogenic introductions. The third clade seems to be the result of a natural colonization from North Africa. All the data indicate that T. mauritanica is a species complex. The Iberian Peninsula, due to its geological complex history, appears to be both a hotspot and a melting pot of biodiversity, with several studies of diverse herpetofauna indicating that cryptic species occur there. More studies of additional species will be needed to develop a comparative phylogeographic framework for the region.

Patterns of alpha, beta and gamma diversity of the herpetofauna in Mexico's Pacific lowlands and adjacent interior valleys

The latitudinal distribution patterns of alpha, beta and gamma diversity of reptiles, amphibians and herpetofauna were analyzed using individual binary models of potential distribution for 301 species predicted by ecological modelling for a grid of 9,932 quadrants of ~25 km2 each. We arranged quadrants in 312 latitudinal bands in which alpha, beta and gamma values were determined. Latitudinal trends of all scales of diversity were similar in all groups. Alpha and gamma responded inversely to latitude whereas beta showed a high latitudinal fluctuation due to the high number of endemic species. Alpha and gamma showed a strong correlation in all groups. Beta diversity is an important component of the herpetofauna distribution patterns as a continuous source of species diversity throughout the region.

Emergent spatial pattern of herpetofauna in Alabama, USA

Analyzing spatial pattern of regional biodiversity and its relationships with environmental factors is important for biodiversity conservation at large scales. The emergent spatial pattern of herpetofauna in Alabama is examined by combining thousands of historical records from 132 species of 24 families and environmental conditions. Our results indicate that species richness of herpetofauna increases with the increase of latitude, while it decreases with the increase of elevation. A negative spatial association exists between amphibians and reptiles on the scale of 10 km2, but 40% of habitats are still shared by amphibians and reptiles at this scale. The highest species richness of herpetofauna is in the Mobile and Baldwin Counties. Power-law relation exists between the county size and the average species richness. Total stream length, and road density are highly correlated with species richness at the county level. With the increase of annual precipitation, species richness decreases. Species richness is higher in the area with the annual average temperature around 17-18 °C. Herpetofaunal diversity in the Coosa/Tallapoosa River, the Alabama River, and the Tombigbee River basins is relatively higher than in the Perdido River and the Escatawpa River basins. The highest species richness exists at the Gulf Coastal Plain, but its species density is the lowest. The highest species richness of herpetofauna exists in the Longleaf-Slash Pine and Loblolly-Shortleaf Pine forests, while lower in Oak- Hickory forest. The emergent spatial pattern may provide important implications for herpetofauna conservation in the face of global climate change and large-scale habitat destruction. The spatial pattern and the possible underlying ecological processes have to be considered for the large scale land zoning and planning.

A rapid assessment survey of the herpetofauna at Befotaka-Midongy National Park, south-eastern Madagascar

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Conserving the Puerto Rican herpetofauna

With a total area of 8900 km2, Puerto Rico is the smallest of the Greater Antilles. It is divided in three physiographic regions or areas of relief: the mountainous interior, the karst region, and the coastal plains and valleys. The island comprises six ecological life zones: subtropical dry forest, subtropical moist forest, subtropical wet forest, subtropical rain forest, lower montane wet forest and lower montane rain forest. The herpetofauna of Puerto Rico consists of 25 species of amphibians (19 native, six introduced) and 56 species of reptiles (52 native, four introduced). The goal of this paper is to describe some of the present studies directed towards the conservation of Puerto Rican herpetofauna. Eleutherodactylus karlschmidti, E. jasperi and E. eneidae have not been seen or heard since 1976, 1981 and 1990, respectively, and are probably extinct. Since 2000, the potential causes of amphibian declines in Puerto Rico have been studied, and a synergistic interaction between climate change (increased dry periods) and disease (chytridiomycosis) have been proposed as an explanation for the patterns observed. Recovery efforts for Peltophryne lemur include a captive-breeding program, reintroductions island-wide educational outreach, protection and restoration of existing habitat, and the creation of new breeding ponds. Among reptiles, the first conservation efforts to protect Epicrates inornatus were limited to trying to halt collection and hunting. However, current strategies to preserve the boa include gathering basic biological information, habitat conservation, and educational outreach. Recent efforts for the conservation of Trachemys s. stejnegeri combine three research approaches to clarify the status of local populations: a mark-recapture-release study, field monitoring of reproductive activity (i. e., nocturnal patrolling to identify nesting activity), and field assessment of the potential impact of introduced species, particularly identification of predatory species and exotic turtles. Recovery initiatives for Cyclura stejnegeri include management of invasive mammals, a headstart program for hatchling iguanas, and the assessment of the etiology of a condition causing blindness in adult iguanas. A reforestation project aimed at recovering a local herpetofaunal assemblage after disturbances in a limestone valley in northern Puerto Rico is discussed. As population sizes of common colonizers such as Eleutherodactylus and Anolis increased, larger forest-interior and predatory species like Epicrates inornatus, Alsophis portoricensis and Anolis cuvieri followed. Finally, the Mona Island marine turtle monitoring program is discussed and compared to other similar programs in Puerto Rico. As these and other similar conservation efforts provide scientifically based management recommendations, we hope to succeed in conserving the diverse herpetofauna that characterizes Puerto Rico.

Community structure and differential responses of aquatic turtles to agriculturally induced habitat fragmentation

Several studies have shown that wetland loss and habitat fragmentation can alter diversity and abundance of herpetofauna, but taxonomic attention has been skewed towards amphibians. We assessed responses of aquatic turtles to features at multiple spatial scales in an intensively farmed region of the Midwestern United States. Spatially hierarchical sampling was conducted from 2001 to 2003 in 35 randomly selected 23-km2 cells throughout the upper Wabash River basin in Indiana. Hoop nets were used at wetlands to capture common snapping turtles (Chelydra serpentina serpentina) (n=258), midland painted turtles (Chrysemys picta marginata) (151), eastern spiny softshells (Apalone spinifera spinifera) (70), red-eared sliders (Trachemys scripta elegans) (59), northern map turtles (Graptemys geographica) (27), false map turtles (Graptemys pseudogeographica pseudogeographica) (6), Blanding’s turtles (Emydoidea blandingii) (3), and stinkpot turtles (Sternotherus odoratus) (3). We examined the degree to which these aquatic species were nonrandomly distributed in 14 landscapes. Assemblages of turtles generally were random and the extent of nestedness was influenced by the diversity of landcover, the proportion of grassland, and the total length of roads in each landscape. The occurrence and abundance of several species also were modeled to test hypotheses regarding the importance of site, patch, and landscape-level variables. Red-eared sliders appeared to be most sensitive to habitat fragmentation, whereas painted turtles, snapping turtles, map turtles, and spiny softshells were less affected. Factors at multiple spatial scales affect turtle distributions, suggesting differential responses to landscape fragmentation.

Herpetofaunal Diversity of Alligator River National Wildlife Refuge, North Carolina

In the past century, habitat alteration and fragmentation have increased dramatically, which increases the need for improving our understanding of how species and biological communities react to these modifications. A national strategy on biological diversity has focused attention on how these habitat modifications affect species, especially herpetofauna (i.e., changes in species richness, community evenness and similarity, and dominant/rare species). As part of this strategy, we surveyed Alligator River National Wildlife Refuge, a coastal, mixed second-growth forested swamp (MFS) and pocosin wetland (PW), in North Carolina for amphibians and reptiles from September 2000 to August 2001. We randomly selected three sites (3 x 3 km) in two major habitat types (MFS, PW) and completed random surveys and trapping using transects, quadrats, nighttime aural road surveys, drift fences, canal transects, coverboards, incidental captures, and evening road surveys. We also col- lected herpetofauna opportunistically throughout the refuge to establish an updated species list. For analysis, we used Shannon-Weiner species diversity (H'), evenness (J'), species richness and species detectability (COMDYN4), and community percent similarity index to determine herpetofaunal community differences. We estimated 39 species in MFS and 32 species in PW (P < 0.10). Species detectability was similar between habitats (0.84 to 0.86). More reptilian species (+ 31%) inhabited MFS than PW, but estimated amphibian species richness was identical (17 spp.). H' was higher (P < 0.0001) for PW (2.6680) than for MFS (2.1535) because of lower J' in the latter (0.6214 vs. 0.8010). Dominance of three Rana species caused lower J' and H' in MFS. Similarity between the communities was 56.6%; we estimated 22-24 species in common for each habitat (95% CI = 18 to 31 spp.). We verified 49 of the 52 herpetofaunal species on the refuge that were known to exist in the area. Restoration of natural water flows may affect herpetofaunal diversity, which may be monitored during a restoration project. Currently, the refuge retains historical levels of herpetofaunal diversity for the region.

Herpetofauna diversity and microenvironment correlates across a pasture–edge–interior ecotone in tropical rainforest fragments in the Los Tuxtlas Biosphere Reserve of Veracruz, Mexico

We evaluated the relationship between amphibian and reptile diversity and microhabitat dynamics along pasture–edge–interior ecotones in a tropical rainforest in Veracruz, Mexico. To evaluate the main correlation patterns among microhabitat variables and species composition and richness, 14 ecotones were each divided into three habitats (pasture, forest edge and forest interior) with three transects per habitat, and sampled four times between June 2003 and May 2004 using equal day and night efforts. We measured 12 environmental variables describing the microclimate, vegetation structure, topography and distance to forest edge and streams. After sampling 126 transects (672 man-hours effort) we recorded 1256 amphibians belonging to 21 species (pasture: 12, edge: 14, and interior: 13 species), and 623 reptiles belonging to 33 species (pasture: 11, edge: 25, and interior: 22 species). There was a difference in species composition between pasture and both forest edge and interior habitats. A high correlation between distance to forest edge and temperature, understorey density, canopy cover, leaf litter cover, and leaf litter depth was found. There was also a strong relationship between the composition of amphibian and reptile ensembles and the measured environmental variables. The most important variables related to amphibian and reptile ensembles were canopy cover, understorey density, leaf litter cover and temperature. Based on amphibian and reptile affinity for the habitats along the ecotone, species were classified into five ensembles (generalist, pasture, forest, forest edge and forest interior species). We detected six species that could indicate good habitat quality of forest interior and their disappearance may be an indication of habitat degradation within a fragment, or that a fragment is not large enough to exclude edge effects. Different responses to spatial and environmental gradients and different degrees of tolerance to microclimatic changes indicated that each ensemble requires a different conservation strategy. We propose to maintain in the Los Tuxtlas Biosphere Reserve the forest remnants in the lowlands that have gentler slopes and a deep cover of leaf litter, a dense understorey, and high relative humidity and low temperature, to buffer the effects of edge related environmental changes and the invasion of species from the matrix.

Effects of Different Burn Regimes on Tallgrass Prairie Herpetofaunal Species Diversity and Community Composition in the Flint Hills, Kansas

The Flint Hills region of Kansas is the largest contiguous area of tallgrass prairie remaining today. Historically, the tallgrass prairie burned every 2–3 yr on average, but current land managers have altered burn regimes, resulting in a range of habitats from annually burned to long-term unburned. We used drift fence/funnel trap arrays and coverboards to estimate species richness, evenness, and diversity of herpetofauna within three different burn regimes: annual, 4-yr, and long-term unburned at Konza Prairie Biological Station, Riley County, Kansas. During the spring and fall of 2003–2004, 315 individuals from 20 species were captured across all burn regimes. Herpetofaunal species richness, evenness, and diversity estimates were not different between the three burn treatments. However, because of species-specific responses to individual burn regimes, community composition was significantly different between the habitats (χ2 = 158.19, df = 20, P < 0.001). Four species exhibited preferences among burn regimes, which differed significantly from independent assortment, with Eumeces obsoletus and Phrynosoma cornutum preferring annual burn treatments, Scincella lateralis preferring 4-yr burn treatments, and Diadophis punctatus preferring long-term unburned treatments. Species-specific responses were likely because of changes in vegetation structure and microhabitat (temperature and moisture content) created through different frequencies of fire disturbances. Maximizing large-scale herpetofaunal diversity across the Flint Hills' rangelands could be accomplished by creating a large number of small scale habitat types through a mosaic style burning plan.