The transformation of industrial heritage buildings into cultural clusters has emerged as a prominent topic of academic research in urban planning, urban studies, heritage conservation, and architecture. Cultural clusters, defined as geographically concentrated cultural activities and organisations, have become a key instrument in urban regeneration, fostering economic growth and cultural development. Despite the benefits that cultural clusters offer in terms of fostering cultural activity, they often prove to be short-lived due to various external factors, including urban regeneration pressures, shifts in policy, and changes in zoning regulations. This thematic issue presents seven case studies that offer insights into the current state of cultural clusters, their transient nature, and the conditions necessary to guarantee their long-term sustainability in industrial heritage sites. The research is particularly relevant in light of the mounting pressure on urban land, where industrial heritage sites are frequently repurposed for residential, commercial, or industrial purposes.