The use of herbicide mixtures is widely adopted by farmers in agriculture worldwide. Glyphosate and ametryn are herbicides commonly applied in tank mixtures, increasing the spectrum of weed control in sugarcane crops. After application, herbicides can reach the soil layer, which is susceptible to retention, transport, and degradation processes. Herbicide interactions in mixtures may affect their bioavailability and persistence in soil, leading to different behavior in the environment compared to when applied alone. This study evaluated the sorption-desorption and transformation of 14C-glyphosate and 14C-ametryn in soil when applied alone or in a tank mixture. The sorption of 14C-ametryn in soil was increased by 25% when mixed with glyphosate (14C-ametryn + glyphosate). This increase in sorption was not evident for 14C-glyphosate when mixed with ametryn (14C-glyphosate + ametryn). The amount desorbed of 14C-ametryn (20%) applied alone was higher than in a mixture (10%). Both herbicide's mineralization half-live time (MT50) was influenced when applied in mixtures. 14C-glyphosate applied alone showed lower MT50 (55 days) compared to 14C-glyphosate + ametryn (119 days). 14C-ametryn also showed lower MT50 when applied alone than when applied with glyphosate (>120 days in both cases). These results suggest that the presence of ametryn in mixture with glyphosate decreases the desorption and mineralization of both herbicides, potentially affecting their bioavailability for weed root absorption and degradation in environment.