Herbicide Active Ingredient Research Articles

Investigating the effects of a novel herbicide active ingredient on soybean growth and yield

Investigating the effects of a novel herbicide active ingredient on soybean growth and yield

Sustainable Paddy Farming in Edirne: Evaluating the Impacts of Excessive Fertilizer and Pesticide Use

This study examines the environmental and economic impacts of chemical fertilizer and pesticide use in paddy fields in Edirne Province, Türkiye. Chemical fertilizers, especially nitrogen and phosphorus, are crucial for boosting paddy yield and ensuring food security. However, recent trends indicate significant potassium deficiency and excessive pesticide use, leading to environmental degradation and higher production costs. Incorrect fertilization techniques and pesticide usage can lower product quality and yield, adversely affecting farmers’ incomes and the national economy. Surveying 139 paddy farmers, the research found that farmers apply the herbicidal active ingredients Tefuryltrione, Clomazone, Tembotrione, and Quinclorac at rates of 129.35%, 34.27%, 18.06%, and 155.29% above the recommended levels, respectively. The excessive use of pesticides costs each farmer an additional USD 925.67, totaling USD 5.32 million for Edirne. The majority of farmers (69.2%) do not conduct soil analysis, indicating a potential gap in adopting the best practices for soil management and fertilization. Farmers use 84% less potassium than recommended while applying nitrogen and phosphorus 13% and 14% above the recommended levels, respectively. The extra cost of fertilizer use in Edirne is calculated as USD 833,135.49. This situation harms the economy and may have adverse effects on the environment and potential health effects on consumers.

Study of Amino Acid-Derived 3-Acyltetramic Acids as Herbicidal Agents.

The growing problem of herbicide resistance necessitates the development of novel herbicidal active ingredients, together with other integrated weed management approaches. Natural products are a major source of inspiration for novel actives. In previous research, we identified a 3-acyltetramic acid of microbial origin that inhibited algal growth in marine biofilms, at least in part through inhibition of photosystem II. In this work, we demonstrate the herbicidal effect of this lead compound and construct multiple libraries to test the impact of the different substituents of the central scaffold in order to study the structure-activity relationships. Among these analogues, the highest activities were found for medium- to long-chain acyl groups and apolar secondary amino acid residues. Finally, we provide first insights into the herbicidal mechanisms and present preliminary field-trial and ecotoxicological results for TA12-Pro, the most active analogue in our library. Together, this research shows the potential of 3-acyltetramic acids for herbicide development.

Uji Sifat Campuran Bahan Aktif Atrazin, Nikosulfuron, Mesotrion pada Beberapa Jenis Gulma

The aim of this research was to know effectiveness and characteristic of mixing herbicide active ingredients atrazine, mesotrion, and nikosulfuron on several weed specieses. The trial conducted in the green house at South Lampung from December 2021 until February 2022. This Research arranged in a Randomized Completely Design. Treatment consists of four types of herbicides with six level of dosage active ingredient, namely of single herbicides is atrazin 200 g/l (0, 25, 50, 100, 200, and 400 g ha-1), nikosulfuron 20 g/l (0, 2.5, 5.0, 10, 20, and 40 g ha-1), mesotrion 40 g/l (0, 5, 10, 20, 40 and 80 g ha-1), and herbicides combination of atrazine, nikosulfuron, and mesotrion 200/20/40 OD (0, 32.5, 65, 130, 260, and 520 g ha-1). The target weed were 3 type of broadleaves weeds (Ageratum conyzoides, Euphorbia hirta, and Richardia brasiliensis), 3 type of grasses weeds (Digitaria ciliaris, Eleusine indica, and Rottboellia cochinchinensis), and sedges weed (Cyperus rotundus). Multiplicative Survival Model method used in this research because atrazin, nikosulfuron, and mesotrion have different mode of action. Results showed that an active ingredient mixture of atrazin 200 g/l, nikosulfuron 20 g/l, and mesotrion 40 g/l has LD50 expectation value of 155.96 g ha-1 and LD50 treatment of 74.27 g ha-1 with the co-toxicity value was 2.10 (co-toxicity >1), it means that the characteristic oh the herbiscides mixture is synergist or not antagonist. Keywords: herbicide mixture, atrazine, mesotrion, nikosulfuron, LD50, Multiplicative Survival Model

Application of Highly Sensitive Immunosensor Based on Optical Waveguide Light-Mode Spectroscopy (OWLS) Technique for the Detection of the Herbicide Active Ingredient Glyphosate.

The herbicide active ingredient glyphosate is the most widely applied herbicidal substance worldwide. Currently it is the market-leading pesticide, and its use is projected to further grow 4.5-fold between 2022 and 2029. Today, glyphosate use exceeds one megaton per year worldwide, which represents a serious environmental burden. A factor in the overall boost in the global use of glyphosate has been the spread of glyphosate-tolerant genetically modified (GM) crops that allow post-emergence applications of the herbicide on these transgenic crops. In turn, cultivation of glyphosate-tolerant GM crops represented 56% of the glyphosate use in 2019. Due to its extremely high application rate, xenobiotic behaviour and a water solubility (11.6 mg/mL at 25 °C) unusually high among pesticide active ingredients, glyphosate has become a ubiquitous water pollutant and a primary drinking water contaminant worldwide, presenting a threat to water quality. The goal of our research was to develop a rapid and sensitive method for detecting this herbicide active ingredient. For this purpose, we applied the novel analytical biosensor technique optical waveguide light-mode spectroscopy (OWLS) to the label-free detection of glyphosate in a competitive immunoassay format using glyphosate-specific polyclonal antibodies. After immobilising the antigen conjugate in the form of a glyphosate conjugated to human serum albumin for indirect measurement, the sensor chip was used in a flow-injection analyser system. For the measurements, an antibody stock solution was diluted to 2.5 µg/mL. During the measurement, standard solutions were mixed with the appropriate concentration of antibodies and incubated for 1 min before injection. The linear detection range and the EC50 value of the competitive detection method were between 0.01 and 100 ng/mL and 0.60 ng/mL, respectively. After investigating the indirect method, we tested the cross-reactivity of the antibody with glyphosate and structurally related compounds.

Evaluation of different weed management strategies including non‐chemical tools and cinmethylin for control of multiple herbicide‐resistant Alopecurus myosuroides

AbstractThe development of resistance in Alopecurus myosuroides populations to different herbicide active ingredients in Europe exacerbates efforts toward its chemical control. In Europe, cinmethylin provides an additional mode of action for the control of A. myosuroides. The objective of this study was to evaluate three resistance weed management (RWM) strategies, including cinmethylin and other pre‐emergence herbicides, for the control of multiple herbicide–resistant A. myosuroides. The RWM strategies used in this study differed in the extent of preventive cultural practices (tillage, crop rotation, sowing date and stale seedbed). Two field trials were conducted at sites with ACCase‐ and ALS‐resistant A. myosuroides biotypes in Germany between 2017 and 2020. Cinmethylin was included in the herbicide regime for A. myosuroides control. The combination of initial inversion tillage, delayed sowing, crop rotation diversification, and stale seedbed reduced the density of A. myosuroides bup to 90% compared to a strategy with pre‐emergence herbicides used as main components in control. At both sites, the amount of viable A. myosuroides seeds in the soil seed bank was reduced by over 90% after a 3‐year trial period in plots with initial inversion tillage. Molecular analyses revealed that the omission of ACCase and ALS inhibitors did not reduce the proportion of resistant A. myosuroides biotypes. Long‐term control of resistant A. myosuroides must be based on a combination of cultural practices that reduce the soil seed bank and suppress the occurrence of A. myosuroides and the use of still‐effective active ingredients for preventing the renewed entry of seeds into the soil.

Application of a Fluorescence-Based Instrument Prototype for Chlorophyll Measurements and Its Utility in an Herbicide Algal Ecotoxicity Assay

Project Aquafluosense was designed to develop prototypes for a modular fluorescence-based instrumental setup for in situ measurement of major water quality parameters. A fluorometer was developed for algal density estimation based on the fluorescent excitation of chlorophyll. The appropriate type of sample holder microplate was determined, along with the need for dark acclimation, prior to the measurements during the instrument’s development. Model species of green (Raphidocelis subcapitata) and blue-green alga (Microcystis aeruginosa) were applied in forms of pure monocultures and their mixtures, and improved analytical limits of detection were achieved (3.70 × 103 cell/mL and 1.13 × 105 for R. subcapitata and M. aeruginosa, respectively). The fluorescence-based determination of algal density was validated by conventional methods, such as cell counting in a Bürker chamber, optical density measurement, and chlorophyll extraction with ethanol. The signals obtained by the fluorometer correlated well with the conventional methods. Pearson r coefficients (applied where the correlation was linear) were ≥0.988 and Spearman ρ coefficients (applied where the correlation was not linear) were >0.976, indicating a strong and positive correlation. The applicability of the developed fluorometer was demonstrated in a growth inhibition ecotoxicity assay on R. subcapitata using the herbicide active ingredient isoxaflutole. During the assay, light intensity (continuous, 104.9 ± 14.9 µE/m2/s), temperature (22 ± 2 °C), pH of algal media (pH = 6–7 for Zehnder and Allen media, as well), and intensity of stirring (continuous, 100 rpm) were controlled. The results indicated that the FluoroMeter Module is applicable for screening algal toxicity: the observed ratio of fluorescence decrease determined by fluorescence induction provided significantly lower toxicity values (EC50: 0.015 ± 0.001 µg/mL) compared to values determined by the optical density (EC50: 0.034 ± 0.004 µg/mL) and chlorophyll a content (EC50: 0.033 ± 0.000 µg/mL).

Cytotoxicity Mechanisms of Eight Major Herbicide Active Ingredients in Comparison to Their Commercial Formulations.

Commercial pesticide formulations contain co-formulants, which are generally considered as having no toxic effects in mammals. This study aims to compare the toxicity of 8 major herbicide active ingredients-namely glyphosate, dicamba, 2,4-D, fluroxypyr, quizalofop-p-ethyl, pendimethalin, propyzamide and metazachlor-with a typical commercial formulation of each active ingredient. Cytotoxicity and oxidative stress capability was assessed in human hepatoma HepG2 cells. Using an MTT assay, formulations of glyphosate (Roundup Probio), fluroxypyr (Hurler), quizalofop-p-ethyl (Targa Super) and dicamba (Hunter) were more toxic than the active ingredient alone. Metazachlor and its formulation Sultan had similar cytotoxicity profiles. Cytotoxicity profiles were comparable in immortalised human fibroblasts. Toxilight necrosis assays showed the formulation of metazachlor (Sultan50C) resulted in significant membrane disruption compared to the active ingredient. Generation of reactive oxygen species was detected for glyphosate, fluroxypyr, pendimethalin, quizalofop-p-ethyl, the formulation of 2,4-D (Anti-Liserons), and dicamba and its formulation Hunter. Further testing of quizalofop-p-ethyl and its formulation Targa Super in the ToxTracker assay system revealed that both products induced oxidative stress and an unfolded protein response. In conclusion, these results show that most herbicide formulations tested in this study are more toxic than their active ingredients in human tissue culture cell model systems. The results add to a growing body of evidence, which implies that commercial herbicide formulations and not just their active ingredients should be evaluated in regulatory risk assessment of pesticides.

Herbicide use in farming and other jobs in relation to non-Hodgkin’s lymphoma (NHL) risk

ObjectivesGiven mixed evidence for carcinogenicity of current-use herbicides, we studied the relationship between occupational herbicide use and risk of non-Hodgkin’s lymphoma (NHL) in a large, pooled study.MethodsWe pooled data from...

Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate.

An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds.

The amino acid substitution Phe-255-Ile in the psbA gene confers resistance to hexazinone in hair fescue (Festuca filiformis) plants from lowbush blueberry fields

AbstractCultivation of lowbush blueberry (Vaccinium angustifolium Aiton), an important crop in the eastern part of North America, is unique, as it is carried out over the course of two consecutive growing seasons. Pest management, particularly weed management, is impacted by this biennial cultural practice. The choice of methods to control weeds is narrow, and such a system relies heavily on herbicides for weed management. Availability of unique herbicide active ingredients for weed management is limited, and available herbicides are used repeatedly, so the risk of developing resistance is acute. Hair fescue (Festuca filiformis Pourr.), a perennial grass weed, has evolved resistance to hexazinone, a photosystem II inhibitor frequently used in lowbush blueberry production. We show that substitution of phenylalanine to isoleucine at position 255 is responsible for a decreased sensitivity to hexazinone by a factor of 6.12. Early diagnosis of resistance based on the detection of the mutation will alert growers to use alternative control methods and thus help to increase the sustainability of the cropping system.

Efficacy of Herbicide Active Ingredients against Aquatic Weeds

This publication answers some common questions and provides efficacy information for all herbicide active ingredients labeled for aquatic use in Florida. Written by Stephen F. Enloe, Ben Sperry, and James Leary, and published by the UF/IFAS Agronomy Department, revised May 2022.

Comparative Toxicity of Herbicide Active Ingredients, Safener Additives, and Commercial Formulations to the Nontarget Alga Raphidocelis Subcapitata.

Chloroacetanilide herbicides are used worldwide to control weeds that affect crops such as corn, soybeans, and cotton. These herbicides are frequently paired with a "safener," which prevents herbicidal damage to the crop without diminishing weed control. Formulated herbicide products that include safeners and other ingredients are infrequently assessed for toxicity. Our goal was to understand the potential toxicity of safeners and herbicide + safener formulations relative to the toxicity of associated active ingredients. We quantified the concentration of safeners in commercially available formulations and tested effects on nontarget algae, Raphidocelis subcapitata, when exposed to individual herbicide active ingredients, safeners, and commercial formulations. The median effective concentrations (EC50s) causing 50% reduction in population growth for the herbicide active ingredients S-metolachlor and acetochlor were 0.046 and 0.003 ppm, respectively. The safeners benoxacor, AD-67, furilazole, and dichlormid were all substantially less toxic than the herbicides and were not toxic at environmentally relevant concentrations. The commercial formulations Dual II Magnum®, Me-Too-Lachlor II®, Harness®, and Surpass EC® all resulted in EC50 values that fell within the 95% confidence interval of the associated active ingredient herbicide. Interestingly, a significant increase in cell size was observed when algae were exposed to all the formulations, herbicides (acetochlor and S-metolachlor), and safener (dichlormid). The safener furilazole caused a significant decrease in cell size, whereas benoxacor and AD-67 had no observed effect on algae cell size. Significant algae cell size effects all occurred at or above the EC50 concentrations for each chemical, suggesting that other morphological effects may be occurring. Importantly, safeners in commercial formulations appeared not to impact toxicity to R. subcapitata compared with the active ingredient alone.Environ Toxicol Chem 2022;41:1466-1476. © 2022 SETAC.

Improved efficiency of an herbicide combining bentazone and terbuthylazine – can weeds be controlled with better environmental safety?

Herbicides are used in agriculture to control the negative impacts of weeds and improve agricultural yields. Authorized herbicidal active ingredients (AIs) were combined in multiple ways to produce distinct formulations targeting diverse weeds.

Estimated herbicide use in the commercial forest sector in South Africa

ABSTRACT This paper examines herbicide use in South African commercial plantations in 2017/18 across three climate zones (cool temperate − CT, warm temperate − WT and subtropical − ST) for three genera (eucalypts, pines and acacias) grown for two end-products (sawn timber and pulpwood). Herbicide information was obtained from 46 timber plantations owned by six forestry companies, comprising 343 872 ha surveyed. The herbicide survey was divided into three climate zones due to increased vegetation growth (and hence more herbicide use) on more productive ST sites compared to moderately productive WT sites, with the lowest vegetation growth on CT sites (lower productivity). The survey was further divided into three distinct vegetation management phases associated with plantation forestry (pre-establishment, re-establishment and post-establishment) to compare herbicide use between genera grown on an annual basis. A total of 188 288 kg (or 0.55 kg ha−1) of herbicide active ingredient (a.i.) was applied in the area surveyed. Glyphosate-based products accounted for 97% of all the herbicides applied, and metazachlor and triclopyr butoxy ethyl ester accounted for 2%. Overall, herbicide use per hectare on an annual basis was highest in the ST zone, followed by the WT and CT zones. For both the CT and WT zones, the general trend was that the pre-establishment phase received noticeably more herbicides, followed by the re- and post-establishment phases (prerepost). This trend remained similar for the genera grown (prerepost), with hardwoods receiving more herbicides than softwoods. In contrast, there was little difference in herbicide use between the re- and pre-establishment phases for the ST zone, with the post-establishment phase being noticeably less. Besides providing benchmark data related to herbicide use in South African plantations, future research should investigate the potential for a reduction in herbicide use in those regions, vegetation management phases and management regimes where it is highest.

In-silico investigation of the efficiency of microbial dioxygenases in degradation of sulfonylurea group herbicides

Sulfonylurea herbicides are widely used to control weeds due to their broad-spectrum activity and low animal toxicity. However, they have negative effects on environment. Microbial dioxygenases are known to degrade aromatic compounds. In this study, three types of dioxygenases (catechol-2,3-dioxygenases, iron dependent homoprotocatechuate 2,3-dioxygenase and manganese dependent homoprotocatechuate 2,3-dioxygenase) were chosen to study molecular interaction with chlorsulfuron and metsulfuron-methyl, respectively. Flexible protein-ligand molecular docking studies were conducted to investigate the efficiencies of these microbial enzymes in degrading the herbicidal residues. Our results indicate homoprotocatechuate 2,3-dioxygenases had more binding affinity than catechol-2,3-dioxygenases, and thus could be claimed to have good efficiency in bioremediation. Further analysis indicated that the higher binding affinity was contributed by the aliphatic residues present at the interacting sites within the glyoxalase domain and its close proximity. Our results provide an important framework for conducting further studies regarding the characterization and analysis of other enzymes involved in the degradation of herbicidal active ingredients. Our study can be considered as a reliable basis for identification and application of microbial enzymes for successful bioremediation of herbicide compounds. This will be very helpful as the number of such toxic compounds continue to increase in the environment.

Efficacy of five herbicide treatments for control ofPyrus calleryana

AbstractCallery pear (Pyrus calleryanaDecne.) is rapidly spreading in the United States, gaining attention in the last two decades as a serious invasive pest. Recommended control methods include foliar, basal bark, cut stump, and hack-and-squirt application of herbicides, but there are few published studies with replicated data on efficacy. Four readily available herbicidal active ingredients and a combination of two active ingredients were tested for control efficacy againstP. calleryanain old-field areas and loblolly pine (Pinus taedaL.) understory. Basal bark applications (triclopyr, triclopyr + aminopyralid), foliar applications (glyphosate, imazapyr), and a soil application (hexazinone) effectively killedP. calleryanawith the exception of hexazinone at one site, where rainfall may not have been optimal. Foliar application of glyphosate provided the most consistent control. Our results demonstrate efficacy of registered herbicide formulations forP. calleryanacontrol in two geographic locations and two habitat types. The need for development of integrated pest management programs forP. calleryanais discussed.

A survey study of the level of pesticide residues in Egyptian exports of vegetables for the period 2015-2019, based on the RASFF databas

Egypt is one of the countries that depend on the export of vegetables and fruits to European Union countries. It follows the procedures imposed by those countries from the organized RASFF that sets special conditions for the quality of those products. Some of the restrictions set by RASFF are concerned with pesticide residues in vegetables and fruits. In this study, data on Egyptian exports of vegetables tracked from the RASFF database organized from 2015 to 2019. The results showed that, about 52 samples banned from the Egyptian exports of vegetables during the period from 2015 to 2019 were due to the high levels of pesticide residues viz: the maximum residues limits .The highest frequent samples under study recorded in the year 2015 at 46.15%, followed by year 2016 and 2017 reaching 21.5%. While the lowest recorded, years were 2018-2019 (5.77 and 3.85 %), respectively. Twenty-four active ingredients recorded in the samples under study. The most detected pesticide groups were insecticides (50%) followed by fungicides (37.5%). All samples were free from any detectable residues of herbicidal active ingredients. While recording, propargite was the highest incidence of samples with 14.46% followed by chlorpyrifos, methomyl, and flusilazole, with percentage frequencies of 12.05%, 10.84%, and 9.64%, respectively. Fourteen out of twenty-four active ingredients registered in the state of Egypt, with the percentage of 58.33 %. The percentage of not registered pesticides was 33.33 % and 8.32% of restricted pesticides use ,of the findings as well as the pesticides revoked by Egyptian authorization.

Efektivitas dan selektivitas beberapa bahan aktif herbisida untuk mengendalikan gulma pada dua varietas tanaman kacang panjang (Vigna sesquipedalis L.)

Field experiments on the effectiveness and selectivity of herbicides use several active ingredients there were Sulfentrazone, Ethyl Pirazosulfuron, and Oxifluorfen with 2 varieties there were Peleton and Kanton tavi. The purpose of the study was to discover the most effective and selective herbicide active ingredients. The study was conducted from February to May 2019. This study used a randomized block design with 7 treatments, namely A (Kontrol), B (Oksifluorfen, peleton), C (Etil pirazosulfuron, peleton), D (Sulfentrazon, peleton), E (Oksifluorfen, kanton tavi), F (Etil pirazosulfuron, kanton tavi), G (Sulfentrazon, kanton tavi). Each treatment was repeated 4 times. The data were analyzed used analysis of variance (ANOVA), further testing used the DMRT test with a 5 % level. The results showed that the application of herbicides made from sulfentrazone, ethyl pirazosulfuron, oxifluorfen in peleton varieties and kanton tavi can suppress weed growth in the research area. The best weed control results were shown by herbicide treatment with sulfentrazone as active ingredient in the kanton tavi variant. The best long bean production results were shown by herbicide treatment with sulfentrazone in peleton varieties. The application of herbicides with active oxifluorfen made no symptoms of phytotoxicity, but decreased the yield of kanton tavi varieties and peleton varieties.

Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis

Glyphosate (GLY) is an herbicidal active ingredient and both in vitro and in vivo studies suggest that GLY alters ovarian function. To determine if a chronic GLY exposure model affected steroidogenesis or folliculogenesis in vivo, postnatal day 42 C57BL6 female mice were orally delivered vehicle control (saline) or GLY (2 mg/Kg) from a pipette tip five days per week for either five or ten weeks. Mice were euthanized at the pro-estrus stage of the estrous cycle. GLY exposure did not impact body weight gain, organ weights, or healthy follicle numbers. In addition, GLY exposure did not affect abundance of ovarian mRNA encoding kit ligand (Kitlg), KIT proto-oncogene receptor tyrosine kinase (c-Kit), insulin receptor (Insr), insulin receptor substrate (Irs1 or Irs2) and protein thymoma viral proto-oncogene 1 (AKT) or phosphorylated AKT. Ovarian mRNA or protein abundance of Star, 3β-hydroxysteroid dehydrogenase (Hsd3b1), Cyp11a1 or Cyp19a were also not altered by GLY. Circulating 17β-estradiol and progesterone concentration were unaffected by GLY exposure. In conclusion, chronic GLY exposure for five or ten weeks did not affect the ovarian endpoints examined herein.