Hepatic Vitellogenin Synthesis Research Articles

Hypoxic and temporal variation in the endocrine disrupting toxicity of perfluorobutanesulfonate in marine medaka (Oryzias melastigma)

Perfluorobutanesulfonate (PFBS) is an emerging pollutant capable of potently disrupting the sex and thyroid endocrine systems of teleosts. However, the hypoxic and temporal variation in PFBS endocrine disrupting toxicity remain largely unknown. In the present study, adult marine medaka were exposed to environmentally realistic concentrations of PFBS (0 and 10 µg/L) under normoxia or hypoxia conditions for 7 days, aiming to explore the interactive behavior between PFBS and hypoxia. In addition, PFBS singular exposure was extended till 21 days under normoxia to elucidate the time-course progression in PFBS toxicity. The results showed that hypoxia inhibited the growth and caused the suspension of egg spawn regardless of PFBS exposure. With regard to the sex endocrine system, 7-day PFBS exposure led to an acute stimulation of transcriptional profiles in females, which, subsequently, recovered after the 21-day exposure. The potency of hypoxia to disturb the sex hormones was much stronger than PFBS. A remarkable increase in estradiol concentration was noted in medaka blood after hypoxia exposure. Changes in sex endocrinology of coexposed fish were largely determined by hypoxia, which drove the formation of an estrogenic environment. PFBS further enhanced the endocrine disrupting effects of hypoxia. However, the hepatic synthesis of vitellogenin and choriogenin, two commonly used sensitive biomarkers of estrogenic activity, failed to initiate in response to the estrogen stimulus. Compared to sex endocrine system, disturbances in thyroidal axis by PFBS or hypoxia were relatively mild. Overall, the present findings will advance our toxicological understanding about PFBS pollutant under the interference of hypoxia.

Effects of sex hormone rescue on gametogenesis in allotriploid crucian carp

Sex hormones play an important role in gametogenesis. During the breeding season, sex hormone levels in the sterile allotriploid crucian carp (3nAT) were significantly lower than those in the fertile diploid red crucian carp (2nRCC). Sex hormone rescue was performed to study the relationship between sex hormones and 3nAT sterility. For three months before breeding season, 3nAT females and males were fed the sex hormones estradiol and testosterone, respectively. During breeding season, hormone-rescued 3nAT fish (re-3nAT) showed normal reproductive behavior, and levels of estradiol in female re-3nAT and testosterone in male re-3nAT were similar to those of the diploid control (2nRCC). The vtg gene, which is associated with the hepatic synthesis of vitellogenin in the female, and the genes spef2, tekt1, and ift27, which are associated with the formation of the sperm flagellum, were significantly upregulated in re-3nAT as compared to 3nAT. Under artificially assisted reproduction, re-3nAT individuals produced a few eggs of different sizes and sperm with abnormal structures. However, few individuals had insemination ability. Neither zygotes produced by self-crossing re-3nAT nor those produced by crossing re-3nAT with the haploid gametes produced by 2nRCC exhibited normal embryonic development. Thus, although sex hormone treatment led to the production of abnormal gametes by re-3nAT, viable offspring did not develop, meaning that re-3nAT remained sterile. These results indicated that sex hormones affected gamete maturation but were not the determinant of triploid sterility.

Does hepatotoxicity interfere with endocrine activity in zebrafish (Danio rerio)?

Vitellogenin (VTG), a well-established biomarker for the diagnosis of endocrine activity in fish, is used in multiple OECD test guidelines (TG) to identify activities of chemicals on hormonal pathways. However, the synthesis of VTG may not only be modified by typical endocrine-related pathways, but also through non-endocrine-mediated processes. In particular, hepatotoxicity, i.e. toxicant-induced impairment of liver structure and function, might influence VTG as a biomarker, since VTG is synthesized in hepatocytes. An intimate understanding of the interplay between endocrine-related and non-endocrine-related pathways influencing VTG production is crucial for the avoidance of erroneous diagnoses in hazard assessment for regulatory purposes of chemical compounds. In order to investigate whether hepatotoxicity may interfere with hepatic VTG synthesis, adult zebrafish (Danio rerio) were exposed to three well-known hepatotoxicants, acetaminophen, isoniazid and acetylsalicylic acid, according to OECD TG 230. Various hepatotoxicity- and endocrine system-related endpoints were recorded: mRNA expression of selected endocrine- and hepatotoxicity-related marker genes in the liver; VTG levels in head/tail homogenates; and liver histopathology. All three test compounds induced significant, but mild single cell necrosis of hepatocytes and transcriptional changes of hepatotoxicity-related marker genes, thus confirming hepatotoxic effects. A positive correlation between hepatotoxicity and reduced hepatic VTG synthesis was not observed, with the single exception of a weak increase in female zebrafish exposed to APAP. This suggests that – in studies conducted according to OECD TG 229 or 230 − it is unlikely that hepatotoxic chemicals will interfere with the hepatic capacity for VTG synthesis.

Effects of increasing temperature due to aquatic climate change on the self-fertility and the sexual development of the hermaphrodite fish, Kryptolebias marmoratus.

In order to assess the effects of increasing temperature on the reproductive performance of fish, different thermal conditions (i.e., 25.0, 26.5, 27.5, 28.5, 30.0°C) were used in this study and the self-fertilizing hermaphrodite fish, Kryptolebias marmoratus, was exposed to these different thermal conditions. During an exposure period of 30 to 150days, the gonadosomatic index (GSI), gonadal development, the levels of plasma 17β-estradial (E2) and testosterone (T), hepatic vitellogenin (VTG) mRNA abundance, and the number of self-fertilized eggs were analyzed. This study confirmed that a high water temperature above 27.5°C led to the suppression of self-fertility of hermaphroditic fish from 30days after exposure. The oocyte quality and maturation would be affected by the disruption of hepatic VTG synthesis at a high water temperature of 30°C, which resulted in the reduced the self-fertility in K. marmoratus. Consequently, this study suggests that elevated water temperature due to aquatic climate change prior to sexual maturation and the onset of spawning can lead to the reproductive dysfunction of hermaphroditic K. marmoratus.

Immunolocalization of hepatic estrogen and progesterone receptors in the female lizard Uromastyx acanthinura

The hormonal regulation of hepatic synthesis of vitellogenin during the annual reproductive cycle was performed for the first time in the deserticole, oviparous, diurnal and herbivorous Uromastyx acanthinura, a lizard belonging to the Agamidae family. In order to elucidate what kind of estrogen receptor is involved in this process, an immunohistochemical study was performed. Changes were obtained in the labeling and cellular distribution of the estrogen and progesterone receptors according to the period of the reproductive cycle and the experimental administration of 17β-estradiol. Only the ERβ subtype was present; it was found in all phases of the cycle with a variable localization: nuclear and cytosolic during vitellogenesis, mainly cytosolic in the female with egg retention (luteal phase) and strictly cytosolic in females at sexual rest. The progesterone receptors were present only at the luteal phase and during sexual rest and disappeared completely from females after 17β-estradiol treatment in sexual rest. Our data suggested that mediation of action of the 17β-estradiol in the vitellogenin synthesis in the lizard U. acanthinura occured via ERβ. PRA and PRB could both be necessary for the negative effect of progesterone on the hepatic synthesis of vitellogenin.

Effects of Lactobacillus rhamnosus on zebrafish oocyte maturation: an FTIR imaging and biochemical analysis

The aim of this study was to verify the effects of probiotic Lactobacillus rhamnosus on zebrafish oocyte maturation using FPA (focal plane array) FTIR imaging together with specific biochemical assays (SDS-PAGE, real-time PCR and enzymatic assay). Oocyte growth is prevalently due to a vitellogenic process which consists of the hepatic synthesis of vitellogenin and its selective uptake during maturation. The administration of L. rhamnosus IMC 501 for 10 days induced chemical changes to oocyte composition, promoting the maturation process. Some interesting biochemical features, linked to protein secondary structure (amide I band) and to phospholipidic and glucidic patterns, were detailed by vibrational analysis. The spectroscopic results were supported by the early increase of the lysosomal enzyme involved in the final oocyte maturation, the cathepsin L. This enzyme increases during follicle maturation, with the highest levels in class IV oocytes. In treated females, class III oocytes showed higher cathepsin L gene expression and enzymatic activity, with levels comparable to class IV oocytes isolated from controls; this can be related to the proteolytic cleavage of the higher molecular mass yolk protein components, as evidenced by SDS-PAGE.

Intracellular, not membrane, estrogen receptors control vitellogenin synthesis in the rainbow trout

The synthesis of vitellogenin, via estrogens, by the liver of female oviparous vertebrates provides the precursor for yolk proteins in developing oocytes. There are two distinct estrogenic transduction pathways in vertebrates that could control vitellogenin synthesis. One provides direct genomic (i.e., nuclear) control in which estrogens bind to estrogen receptors (ERs) that function as transcription factors within the cell nucleus. The other involves a non-genomic pathway initiated by estrogens binding to membrane-bound ERs at the cell surface. The objective of this paper was to determine which ER transduction pathway regulates hepatic vitellogenin synthesis in rainbow trout. For this study an estrogenic molecule, 17α-ethynylestradiol (EE2), was conjugated to a peptide moiety (PEP) to make 17α-ethynylestradiol-peptide (EE2-PEP) to bind to membrane-bound ERs. This was compared with EE2 that is capable of crossing the cell membrane and binding to intracellular ERs. An in vivo experiment using male rainbow trout injected with either EE2-PEP or EE2 demonstrated that only EE2 stimulated a significant increase in plasma vitellogenin concentrations. This was further confirmed by treating male rainbow trout hepatocytes in primary culture for 24 h with PEP, EE2-PEP or EE2. Only the EE2 treatment resulted in significantly higher vitellogenin expression in trout hepatocytes. These results demonstrate that estrogens must gain entry into hepatocytes to bind to intracellular ERs in order to stimulate vitellogenin synthesis. While this study cannot conclude that a membrane ER system is absent in the rainbow trout liver, it has established that the liver synthesis of vitellogenin in rainbow trout is regulated by intracellular ERs.

Stressors, glucocorticoids and ovarian function in teleosts

The purpose of this overview is to re-examine the postulated direct and indirect actions of glucocorticoids on ovarian function in teleosts. The re-examination is undertaken in light of recent advances in the understanding of the stress response itself, the mode of action of the hypothalamus-pituitary gland-ovarian (HPO) axis, the mechanisms of control of oestrogen-dependent hepatic vitellogenin (VtG) secretion and the apparent roles of corticotrophin-releasing hormone (CRH) and CRH-related factors in the regulation of feeding activity. Many of the results of different studies, particularly whole-animal studies, are conflicting, and little is known as to whether the hormone acts directly on various components of the HPO axis or indirectly by virtue of redirection of energy resources away from ovarian growth to provide a source of metabolic resources for other organ systems involved in the physiological stress response. In vitro studies provide some new insights into the direct actions of glucocorticoid on hepatic VtG synthesis and ovarian follicle steroidogenesis, but even here, in some studies the cellular sites of action of these hormones is not altogether clear. The overview emphasizes the complexity of the stress response, the complexity of the regulation of glucocorticoid-dependent gene expression and the extensive interactive nature of the HPO with other hypothalamus-pituitary gland-peripheral endocrine gland axes, such as the thyroid (HPT), 'somatic' (GH-IGF) and interrenal tissue (HPI) axes.

PTHrP potentiating estradiol-induced vitellogenesis in sea bream ( Sparus auratus, L.)

In fish, vitellogenin is an important nutritional precursor protein produced solely in the liver and released into the blood where it binds calcium. In the gilthead sea bream ( Sparus auratus) 17β-Estradiol (E 2) plays an important role in the synthesis of vitellogenin, but also the pituitary hormones prolactin (PRL) and growth hormone (GH) can stimulate vitellogenin induction in fish. Considering the emerging involvement of PTHrP in fish calcium metabolism and the importance of calcium regulation in reproduction, we investigated the possible role of PTHrP in vitellogenesis. E 2-naïve and E 2-primed sea bream hepatocytes were used in an in vitro primary hepatocyte culture and stimulated with a recombinant sea bream PTHrP (sbPTHrP) to establish the contribution of sbPTHrP alone or in combination with E 2 to the regulation of hepatic vitellogenin synthesis. Hepatocytes stimulated solely with sbPTHrP were not affected in their vitellogenesis. However, in hepatocytes stimulated with E 2 in combination with sbPTHrP a higher vitellogenin production was seen than with E 2 alone. It is concluded that sbPTHrP has a potentiating effect on estradiol stimulation of vitellogenin production by sea bream hepatocytes. The sea bream provides a unique model where vitellogenesis regulation can be studied on E 2-naïve liver cells, both in vivo and in vitro.

Androgen action on hepatic vitellogenin synthesis in the eel, Anguilla japonica is suppressed by an androgen receptor antagonist

Involvement of additional hormones other than estrogen in the control of vitellogenin (Vg) synthesis has been suggested in fish. However, no satisfactory explanation on the mechanism of the action of these hormones has been reported. In this study, we have exploited the possibility of androgen receptor mediation during the androgen action on the pathway of Vg synthesis. Hepatocytes were prepared from sexually immature Japanese eel Anguilla japonica and treated with estradiol-17β (E 2), 17α-methyltestosterone (MT), growth hormone, tamoxifen or flutamide, or in combination of these. Spent culture media were analysed by SDS-PAGE for Vg detection. Results from the chemical treatments demonstrated the necessity of E 2 as the primary factor for Vg synthesis and requirement of additional hormones for the full expression of Vg. The effects of E 2 and MT were effectively blocked by tamoxifen, an estrogen receptor antagonist and flutamide, an androgen receptor antagonist, respectively, indicating ER-mediated estrogen action and AR-mediated androgen action on Vg synthesis in this species.

Synergistic role for pituitary growth hormone in the regulation of hepatic estrogen and progesterone receptors and vitellogenesis in female freshwater turtles, Chrysemys picta

Previous studies using the freshwater turtle Chrysemys picta have demonstrated that estradiol, progesterone (P), testosterone (T), and growth hormone (GH) regulate hepatic vitellogenin synthesis, suggesting a multihormonal regulation of vitellogenesis in the turtle. In this study we further investigated the interaction between estradiol-17β (E) and growth hormone in the regulation of vitellogenin (vtg) in hypophysectomized post-reproductive female turtles ( C. picta). Northern blot analysis was used to monitor the changes in vtg mRNA, ER mRNA, and PR mRNA expression; Western blot to determine changes in PR isoform expression and a homologous ELISA for measurement of plasma vtg. Compared to sham-operated controls, hypophysectomy did not reduce the hepatic levels of any parameters below the seasonal norm. Changes in these parameters in hypophysectomized animals after administration of GH alone, estrogen alone, or GH in combination with estrogen were well correlated. The effect of estrogen alone was greater than that of GH, and elevated all end-points analyzed. With the exception of plasma vitellogenin, the effect of GH plus estrogen was significantly greater than either hormone alone. In contrast to changes in ER mRNA, vtg mRNA, and vtg protein, the effect of estrogen and GH plus estrogen treatments on PRA mRNA and PRC mRNA, although significant, was relatively modest. However, changes in PRA and PRB protein were large (5- to 10-fold), and of similar magnitude to the changes in ER mRNA, vtg mRNA, and plasma vtg. Further, PRA and PRB protein levels appeared to be differentially affected. Thus, in sham and normal animals, only PRB was detected, and the levels were similar. After GH administration, PRB increased 4- to 5-fold, and PRA became detectable. Estrogen increased both isoforms of PR equally (approximately 6-fold), and some synergism was apparent when the two hormones were administered together, PR levels being the highest in this group (about an 8-fold increase). The results suggest that although estrogen is the primary regulatory factor involved in activation of vitellogenin synthesis, basal levels of all primary components of signaling pathways involved in vitellogenesis measured here may be maintained in the absence of either estrogen or GH, and that GH alone can activate transcription of some of these important transcription factors.

Multihormonal Control of Vitellogenesis in Lower Vertebrates

The comparative approach on how and when vitellogenesis occurs in the diverse reproductive strategies displayed by aquatic and terrestrial lower vertebrates is presented in this chapter; moreover, attention has been paid to the multihormonal control of hepatic vitellogenin synthesis as it is related to seasonal changes and to vitellogenin use by growing oocytes. The hormonal mechanisms regulating vitellogenin synthesis are also considered, and the effects of environmental estrogens on the feminization process in wildlife and humans have been reported. It is then considered how fundamental nonmammalian models appear to be, for vitellogenesis research, addressed to clarifying the yolkless egg and the evolution of eutherian viviparity.

Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption

The oocyte is the starting point for a new generation. Most of the machinery for DNA and protein synthesis needed for the developing embryo is made autonomously by the fertilized oocyte. However, in fish and in many other oviparous vertebrates, the major constituents of the egg, i.e. yolk and eggshell proteins, are synthesized in the liver and transported to the oocyte for uptake. Vitellogenesis, the process of yolk protein (vitellogenin) synthesis, transport, and uptake into the oocyte, and zonagenesis, the synthesis of eggshell zona radiata proteins, their transport and deposition by the maturing oocyte, are important aspects of oogenesis. The many molecular events involved in these processes require tight, coordinated regulation that is under strict endocrine control, with the female sex steroid hormone estradiol-17β in a central role. The ability of many synthetic chemical compounds to mimic this estrogen can lead to unscheduled hepatic synthesis of vitellogenin and zona radiata proteins, with potentially detrimental effects to the adult, the egg, the developing embryo and, hence, to the recruitment to the fish population. This has led to the development of specific and sensitive assays for these proteins in fish, and the application of vitellogenin and zona radiata proteins as informative biomarkers for endocrine disrupting effects of chemicals and effluents using fish as test organisms. The genes encoding these important reproductive proteins are conserved in the animal kingdom and are products of several hundred million years of evolution.

Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons

During their 3–4 first years of life, triploid sea bass Dicentrarchus labrax grew in a similar fashion to diploids in fork length but more slowly than diploids (P<0·05) in body weight, even when the diploids reached full sexual maturity. However, from 48–53 months of age triploids exhibited non‐significantly higher instantaneous growth rates, and thus when fish were 4 years or older, differences in weight with diploids were no longer apparent, suggesting that triploidy could be of benefit in the culture of large (>1 kg) sea bass. The condition factor was reduced in both ploidies during the spawning season which took place in winter when the temperature was low. These observations suggest that any growth advantage in triploids, which were functionally sterile, may be offset by unfavourable environmental conditions. Thus, the potential gain of triploid fish, because they do not direct energy to gonadal growth, could not overcome the effects of low temperature on somatic growth, which coincided with the spawning season. This suggests that the low growth of this species during winter is more a consequence of low temperature than of the energetic cost associated with reproduction. On the other hand, the lower hepatosomatic index in triploid females in contrast to diploid females might be indicative of the lack of gonadal oestradiol‐mediated hepatic synthesis of vitellogenin. Also, erythrocyte and haematocrit measurements showed an increased nuclear and cellular volume in triploids, but with similar cell numbers to those of diploids, respectively.

Gonadotropins and reproductive function in the anuran amphibian, Rana esculenta.

In this study, the measurement both of peripheral gonadotropins (FSH and LH) and of sex steroids in male and female wild anuran, Rana esculenta, was performed during the annual reproductive cycle; moreover, the role of gonadotropins in the vitellogenic process and in ovarian steroidogenesis was investigated through in vitro experiments. LH plasma changes in males showed high values during autumn-winter months and during the mating period, when high androgen levels were found. Conversely, for the first time in male vertebrates, a clear correspondence between plasma FSH and estradiol-17beta (E2) was shown. In females, FSH peak values were found at the beginning of the mating period in parallel with those of plasma vitellogenin (VTG) and E2; in contrast, high LH levels went together with ovarian weight (gonadosomatic index), which is considered a good marker for the plasma sequestration of VTG by growing oocytes. The in vivo results are corroborated by in vitro studies showing the direct effects of both FSH and LH in inducing hepatic VTG synthesis and release in the culture media. Lastly, although it is not yet known whether or not FSH and LH have separate functions in amphibians, it was clearly shown that they induce ovarian steroid production. These results are discussed in terms of the high seasonality previously demonstrated in this wild frog.

Multihormonal control of vitellogenin mRNA expression in the liver of frog, Rana esculenta

In Rana esculenta in an in vitro system, hepatic vitellogenin synthesis can be induced by growth hormone in both sexes. In this study: (1) the ability of this hormone to induce transcription of the VTG gene was determined, and (2) this ability was compared with that of estradiol-17β. The results indicate that growth hormone stimulates VTG mRNA transcription both in vivo and in vitro, in both sexes. The levels of mRNA are related to protein levels in the medium. In addition, seasonal variation occurs in the VTG gene transcription under growth hormone and estradiol-17β; indeed the more active inducer was growth hormone during the reproductive period and estradiol-17β during the prereproductive phase.

In-vitro effects of mammalian and amphibian prolactins on hepatic vitellogenin synthesis in Rana esculenta

Male and female Rana esculenta liver was induced in an in-vitro system by homologous and Rana catesbeiana pituitary to synthesize and release vitellogenin, a lipoglycophosphoprotein precursor of yolk proteins, lipovitellins and phosvitins, in oviparous vertebrates. In the present experiments, the action of prolactin on hepatic vitellogenin synthesis and release was investigated, using ovine prolactin and Rana catesbeiana prolactin. The effects of prolactin on hepatic vitellogenin synthesis displayed different trends related to sex; male liver was found to be more responsive than female liver to both ovine and frog prolactin; more-over, the response to prolactin was dose-related (r = 0.998; P < 0.05) in male but not in female liver. In both sexes, a high degree of seasonality in the responsiveness of the liver was found, since the vitellogenin levels induced by prolactin during the winter phase were significantly (P < 0.001) higher than those produced during the summer phase. Thus, there was no significant difference between the action of ovine and frog prolactin on vitellogenin synthesis; in fact, mammalian prolactins are structurally similar with regard to nucleotide and amino acid sequences. The direct action of prolactin on hepatic vitellogenin synthesis in the frog Rana esculenta is discussed, on the basis of the role played by prolactin as an important growth modulatory hormone in fetal and adult tissues.

Regulation of hepatic vitellogenin synthesis in the little skate (Raja erinacea): Use of a homologous enzyme‐linked immunosorbent assay

AbstractAn enzyme‐linked immunosorbent assay (ELISA) was developed to detect vitellogenin in skates. The antibody raised against the yolk protein lipovitellin was shown to recognize a sexspecific, estrogen‐inducible 205 kD protein in plasma, previously identified in skate as vitellogenin. The detection limit of the assay was 31.3 ng/ml, similar to that of radioimmunoassays for vitellogenin detection in other species. Using the assay, vitellogenin levels were shown to be highest (241.8 ± 26.5 ug/ml) in the ovarian follicular phase, decreasing approximately 1‐2 days before ovulation to a low (173.1 ± 22.9 ug/ml) during the luteal phase. Vitellogenin levels increased again approximately 1‐2 days before oviposition to 259.8 ± 38.8 ug/ml.The effects of estrogen and progesterone on hepatic vitellogenin production in normal and hypophysectomized male skates were also investigated. Plasma vitellogenin was not detectable in either intact or hypophysectomised male skates, but increased with estradiol treatment (intact: 48.2 ± 4.9 ug/ml vs. hypophysectomised 252 ± 10.6 ug/ml at day 8). Progesterone treatment significantly (P &lt; .01) attenuated the effect of estrogen on plasma vitellogenin levels in both intact (17 ± 2.1 ug/ml) and hypophysectomized (44.0 ± 9.4 ug/ml) male skates. © 1993 Wiley‐Liss, Inc.

Inhibition of ovarian microsomal aromatase and follicular oestradiol secretion by imidazole fungicides in Rainbow trout

The knowledge of critical sites of interaction between pollutants and fish is a major means of investigation in order to obtain early advance knowledge of adverse alterations and ecotoxicological risks. In fish, the ovarian secretion of oestradiol-17β has been demonstrated to govern hepatic synthesis of vitellogenin, a lipoprotein corresponding to the major part of embryonic trophic reserves. Oestradiol synthesis is catalysed by aromatase, a cytochrome P450-dependent enzymatic activity. Effects of different imidazole fungicides, well known as potent inhibitors of cytochrome P450s, were tested in vitro. A concentration-dependent inhibition of the microsomal aromatase was observed with an inhibiting potency order as follows: clotrimazole (Inhibition 50%: 5–10 −7 M) > prochloraz and imazalil (I 50%: 5–10 −6 M) > ketoconazole (I 50%: 5–10 −4 M ). Secretion of oestradiol from cultured follicles was reduced after exposure to 10 −6 M prochloraz in the culture medium.

Hormonal control of in vitro vitellogenin synthesis in Rana esculenta liver: Effects of mammalian and amphibian growth hormone

Estradiol 17-β is known to induce hepatic synthesis and secretion of vitellogenin in all species studied and in Rana esculenta, previous experiments demonstrated the involvement of pituitary in these processes; indeed, in addition to estradiol 17-β, homologous pituitary homogenate directly stimulated male and female liver to produce vitellogenin in tissue cultures. Therefore, the effect of ovine growth hormone (o-GH) and Rana catesbeiana growth hormone (f-GH) on hepatic vitellogenin synthesis was investigated. In the present in vitro experiments, both o-GH and f-GH positively stimulated vitellogenin synthesis, in female and male liver, in a dose-related fashion. No significant differences were found in VTG levels induced by o-GH and f-GH. The GH stimulatory effects, found during the different phases of the reproductive cycle, displayed different trends related to season and sex.