Background: The aim is to define predictive factors for the onset of biliary stones (BS) after liver transplantation (LT). Methods: We studied the onset of BS in 390 LTs from 2004 to 2014. The study was limited to 317 LTs with duct to duct biliary anastomosis on a t-tube and 47 hepatico-jejunal anastomoses. Data concerning the donor, the transplant and the recipient were collected. We performed an univariate analysis, and then a multivariate analysis. P= .05 was considered statistically significant. Results: BS occurred in 14/364 grafts (3,8%). Predictive factors for BS in univariate analysis were high Donor Body mass Index, hepatocellular disease (vs tumors), hepatic artery thrombosis and biliary stenosis. Only biliary stenosis was statistically significant in the multivariate analysis (p=.0001, OR=17.077, 95% CI 4,222-69,068). A second analysis involved 51/364 grafts with biliary stenosis, according with the onset or not of BS. Predictive factors for the onset of BS in univariate analysis were recipient female sex and ischemia time > 10 hrs; in a multivariate analysis including also donor sodiemia and hepatic artery thrombosis, female sex (p=0,018, OR 11.297, 95% CI 1,516-84,155) and ischemia time > 10 hrs (p=.028, OR 10,147, 95% CI 1,288-79,961) remained statistically significant. Conclusion: Biliary stenosis is confirmed as a main risk factor for the onset of BS after LT. In grafts with biliary stenosis predictive factors for BS are recipient female sex and ischemia time > 10 hrs.