Hepatectomy In Rat Liver Research Articles

Effect of carvacrol on IL-6/STAT3 pathway after partial hepatectomy in rat liver.

Carvacrol is a component in the essential oil of Lamiaceae family. Beside its antihepatotoxic and hepatoprotective properties it is known to be a contribution to liver regeneration. This study aimed to investigate the effects of carvacrol in rat liver regeneration after 70 % partial hepatectomy (PHx) through interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) and mitogen-activated protein kinases (MAPK) pathways. In our study six groups were formed from 3-month old Wistar albino male rats. Group I and II were Sham operated; Group III and IV received 1 mL 0.9 % NaCl; and Group V and VI were the groups that were treated with 73 mg/kg carvacrol. Group III, IV, V, and VI animals underwent 68-70 % PHx. Dissection was performed 24 and 48 after surgical procedure. Serum Alanine transaminase (ALT) levels were determined. The liver regeneration rate (RR) was calculated. Histopathological analysis was performed by Hematoxylin(et)Eosin staining and the proliferation by proliferating cell nuclear antigen (PCNA) immunohistochemical staining in liver samples. In the liver, certain genes expressions in IL-6/STAT3 and MAPK pathways with RT-PCR analysis and protein expression with Western blot analysis were measured. Carvacrol showed a positive effect on hepatocyte proliferation and liver regeneration 24 and 48 hours after PHx (Tab. 1, Fig. 7, Ref. 39).

Apoptosis induced by boric anhydrite (B2O3) after partial hepatectomy in rat liver.

Boron is one of the important elements that have a cell-growth suppressing effect. The apoptotic effects of B(2)O(3) that were investigated in rats on liver regeneration following 70 % partial hepatectomy (PH). Wistar albino male rats were used and divided into 4 groups (n = 7). The Saline control groups were given only a single dose of saline; the B(2)O(3)-treated groups that were only given a single dose of 1800 mg.kg(-1) B(2)O(3) by means of intraperitoneal injections following hepatectomy. Three and 6 hours after surgical procedures, all the groups were dissected and liver tissue samples were taken from the groups for NF-κB for caspase-3 gene and protein levels investigation by RT-PCR and TaqMan Protein Assay and histological analyses by TUNEL assay. B(2)O(3)-treated animals were examined and it was observed that NF-κB levels were decreased; however, caspase-3 gene expression and protein levels were increased significantly. This study demonstrated that B(2)O(3) induces caspase-3 and inhibits NF-κB at the early stage of liver regeneration (Fig. 4, Ref. 26).

Hepatocyte senescence induced by radiation and partial hepatectomy in rat liver

Purpose: Exposure to radiation primes the liver for extensive replacement of the resident parenchymal cells by transplanted hepatocytes. The mechanisms underlying this repopulation remain to be clarified. In these studies, we examined the possible occurrence of cell senescence in vivo following radiation-associated preconditioning of the host liver.Materials and methods: Fischer 344 rats underwent external-beam, computed-tomography-based partial liver irradiation. A single dose of 25 Gy was delivered to the right liver lobes (40% of liver mass). An additional group of animals received a 1/3 partial hepatectomy (removal of the left anterior lobe) four days after irradiation. Non-irradiated groups served as controls. All rats were sacrificed four weeks after the initial treatment.Results: The irradiated livers displayed several markers of cell senescence, including expression of senescence-associated-β-galactosidase (SA-β-gal), increase in cell size, and up-regulation of cyclin-dependent kinase inhibitors (CDK-I) p16 and p21. Furthermore, quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis revealed activation of the senescence-associated secretory phenotype (SASP), including the cytokines interleukin 6 (IL6) and 1α (IL1α). The senescence-related changes were more prominent in rats undergoing partial hepatectomy (PH) following irradiation (IR).Conclusions: We conclude that priming with radiation for liver repopulation results in the induction of cell senescence and the up-regulation of a senescence-associated secretory phenotype. The latter can contribute to the extensive growth of transplanted cells in this system.

Pretreatment with recombinant human interleukin 2 (rhIL-2) Up-regulates PCNA-positive cells after partial hepatectomy in rat liver

We prepared recombinant human interleukin-2 (rhIL-2) and studied its pretreated influence on liver regeneration and the blood profile in partially (67%) hepatectomized (PH) male Sprague-Dawley rats. Rats were injected in the tail vein with rhIL-2 three times per day for 3 consecutive days and 67% underwent a partial hepatectomy (PH). Five days after the PH, liver tissue and blood samples were analyzed for liver regeneration and hematological changes. The weight of the liver in the rhIL-2 pretreated groups increased in a dose-dependent manner; with the highest treatment (24 × 104 IU/kg), the maximum liver weight of 88.6% was exhibited. The control group showed a gradual increase to 76.3% of the original liver weight. A histological analysis of the liver showed an increase in proliferating cell nuclear antigen (PCNA)-positive cells in rhIL-2 pretreated rat livers. The rate of hepatocyte proliferation also increased significantly in primary cultured rat liver cells following rhIL-2 treatment. These results suggest that pretreatment with rhIL-2 may play adjuvant roles in liver regeneration after PH.

Changes of TGF-a, HGF, PCNA and IGFBP-1s mRNA after partial hepatectomy in rat liver

Changes of TGF-a, HGF, PCNA and IGFBP-1s mRNA after partial hepatectomy in rat liver