To address the challenges associated with airflow organization in chicken coops within the Chinese poultry industry, this study conducted on-site measurements and utilized Computational Fluid Dynamics (CFD) simulations. The validity of the simulation results was confirmed through root mean square error analysis in comparison to experimental data. Subsequently, the study assessed the influence of different chicken rack arrangements within the coops, both when wet curtains were closed and opened. The findings reveal that, when wet curtains are closed, the internal coop layout significantly impacts chicken welfare, especially concerning layout changes relative to coop length. When wet curtains are open, the impact of coop layout and a 10-meter reduction in coop length on chickens is approximately equivalent. The arrangement of chicken racks within the coop has a profound effect on airflow organization. Therefore, when designing coops, careful attention to chicken rack placement is essential to ensure proper airflow organization.