Standardization and quantification are vital in every comparative study in medicine and biology. The instrumentation and methods used for achieving these purposes in our investigations into various aspects of low-power lasers and injuries to peripheral nerves (PN) are described. The system enables quantified and localized manoeuvers and measurements in handy, small animal models and can be easily adapted to other procedures. The sciatic nerve of rats was used as the in vivo model. Haemostatic forceps, fitted with calibrated strain gauges, were used to inflict pore-planed, quantified and localized crush injuries upon the nerve. The electrical activity in the nerve before, during and after various treatments was measured in vivo using a multiple electrode rack that enabled stimulation and recording of action potential (AP). Examples of tests using the recommended instrumentation and methods are described. These tests enable us, due to the use of standard tools and methods, to assess and compare the efficiency of red, green and infra-red lasers in nerve stimulation and regeneration. Some of the highly significant effects are mentioned.