We described three cases of macular holes with an epiretinal membrane that developed after triamcinolone-assisted vitrectomy for proliferative diabetic retinopathy (PDR). In case 1, vitrectomy was performed for vitreous hemorrhage and tractional retinal detachment. In case 2 and 3, vitrectomy was performed for vitreous hemorrhage. Triamcinolone acetonide was used to visualize the vitreous during surgery and the posterior vitreous cortex was completely removed. Macular hole occurred 18, 9, and 1 months after the initial vitrectomy in cases 1, 2, and 3, respectively. In all cases, additional surgery was performed and closure of the macular hole was achieved. Macular hole with epiretinal membrane occurred after triamcinolone-assisted vitrectomy for PDR in three cases. Hole closure was achieved after additional vitreous surgery. Epiretinal membrane and macular hole might occur even in cases in which the posterior vitreous cortex has been removed completely during triamcinolone-assisted vitrectomy for PDR.