Abstract Background and Aims Septic shock is one of the most frequent causes in Intensive Care Unit (ICU) admission and is related to a very high risk of mortality. About one third of patients with sepsis develops acute kidney injury (AKI) which contributes to a worsening prognosis. AKI due to sepsis is the result of a dysregulated host immune response to infection, with the production of inflammatory mediators and cytokines that cause hemodynamic alterations, endothelial damage, apoptosis and, finally, immunoparalysis. The Jafron HA380 cartridge has been specifically designed for use in clinical conditions characterized by cytokine storm such as sepsis. Given the growing application of these hemoadsorption device in cases of septic AKI in ICU, an unsolved problem is whether these polymers adsorb drugs, including antibiotics. In vitro experiments were conducted to determine its adsorption capacity towards Linezolid (LZD) antibiotic. Method In vitro circulation was performed using a dedicated testing platform Galileo. A customized cartridge was built assembling mini-module components scaled in dimension towards HA380 and filled with 75 g of HA380 beads (25% of the regular size cartridge). Peristaltic pump was set at 250 mL/min. Four circulations were performed varying the initial mass of Linezolid in the tested solution (200, 400, 600, 1200 mg). Samples were collected after the first passage (FP) through the cartridge and every 5 or 10 minutes and Linezolid concentrations were measured. Adsorption was assessed considering the Removal Ratio (RR) and its kinetics. Results In vitro circulation confirms the affinity of beads material in binding Linezolid molecules. The kinetics shows an instantaneous and rapid adsorption in the very first part of the experiments. After that the adsorption rate decreases due to the decrease of antibiotic available in the solution. Among all the experiments, after the first passage of the solution through the cartridge, a RR higher than 70% has been achieved. After 100 passages of the solution into the cartridge, the adsorption reached about the 100% of RR. None of the amounts of Linezolid tested led to the cartridge saturation. A linear behavior between the mass injected in the solution and the mass adsorbed can be ascribable to the linear phase of Langmuir isotherm (see Fig.). Conclusion HA380 adsorbs significant amounts of Linezolid. The linear trend of the isotherm reveals a direct proportionality between binding sites of the sorbent material and the Linezolid tested. Further investigation using a higher quantity of Linezolid is necessary to reach the saturation of the cartridge and to understand the sorbent material capacity. These preliminary results highlight that there is an interaction that could affect the clinical outcome.